Anatomia de folhas, brácteas e escapos de Paepalanthus Sect. Diphyomene Ruhland (Eriocaulaceae, Poales)

As Eriocaulaceae brasileiras encontram-se distribuídas principalmente nos campos rupestres que se estendem de Minas Gerais à Bahia, na Cadeia do Espinhaço. Paepalanthus Mart. é o maior gênero da família e apresenta cerca de 450 espécies, sendo que Paepalanthus sect. Diphyomene Ruhl. possui cerca de 20 espécies que já pertenceram a diversas categorias taxonômicas. Com o objetivo de correlacionar a anatomia e a taxonomia desta seção, além de levantar caracteres diagnósticos para suas espécies, foi estudada a anatomia de folhas, brácteas e escapos. Folhas e brácteas de Paepalanthus sect. Diphyomene apresentam diferenças na forma e no espessamento das células epidérmicas; no espessamento do mesofilo; na disposição dos feixes vasculares; presença ou ausência de hipoderme; diferente forma e composição de parede das células da extensão de bainha do feixe vascular; forma e constituição da margem e presença ou ausência de parênquima aqüífero na face adaxial. No escapo, foram observadas diferenças quanto ao número e contorno de costelas e extensão da medula. Assim, a proposta de divisão de P. sect. Diphyomene em duas categorias é corroborada. São caracteres diagnósticos para as espécies: presença de parênquima aquífero na face adaxial e ausência de extensão de bainha do feixe vascular em folhas de Paepalanthus urbanianus; presença de feixes vasculares distribuídos em ordem decrescente de tamanho em direção à margem em folhas e brácteas e contorno triangular do escapo em P. flaccidus, presença de nove costelas em escapos de P. acanthophyllus e dez em P. macer. Foi elaborada uma chave de identificação das espécies de Paepalanthus sect. Diphyomene com base nas características anatômicas levantadas

Estudo químico e avaliação da atividade antimicrobiana de fungos endofíticos associados à Paepalanthus chiquitensis (eriocaulaceae) e efeitos de moduladores químicos epigenéticos no cultivo de Pochonia chlamydosporia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anatomia floral e ontogenia de espécies dímeras de Paepalanthus Mart. (Eriocaulaceae, Poales) e seu significado evolutivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo químico dos escapos de Paepalanthus geniculatus Kunth

Eriocaulaceae species are popularly known as sempre-vivas. This family comprising approximately 1.200 species divided into 10 genera, with high endemic levels. Paepalanthus genus has high incidence in the Espinhaço Range region and the report of biological activities. Despite the large number of previous studies with Eriocaulaceae species, Paepalanthus geniculatus has no studies about their scapes, which demonstrates the need for new research to identify their chemical and biological composition. The aim of this work was to study the chemical composition of P. geniculatus ethanolic extract seeking the isolation and identification of metabolites and evaluate the radical scavenging activity of the extract and isolated substances. P. geniculatus were collected in Serra do Cipó-MG in 2013 (Voucher: SANO 3193) and the scapes were dried, crushed and the powder was percolated with ethanol. With the etanolic extract it is carried out a fractionation by gel permeation chromatography, yielding fractions analyzed by TLC assay. The fraction J89 (45,6 mg) was purified by semipreparative HPLC-PDA, resulting in the isolation of substance S1. The fractionation was also performed by medium pressure liquid chromatography, yielding fractions analyzed by TLC assay. The fraction number 4 (92,6 mg) were purified by semipreparative HPLC-PDA resulting in the isolation of substances S2 and S3. The substances S1 and S3 were analyzed by mono and twodimensional NMR, resulting in the identification of 3,4-dihydroxybenzoic acid (protocatechuic acid) and the flavonoid 6-hydroxyquercetin-7-O-β-Dglucopyranoside. The substance S2 were identified by comparison with standards, were it was possible to determine the presence of the flavonoid 6-hydroxy-7- methoxyquercetin-3-O-β-D-glucopyranoside. The evaluation of radical scavenging activity for the extract and the isolated substances using DPPH, showed consistent activity to S1...

Estudo químico dos escapos de Paepalanthus geniculatus Kunth

Eriocaulaceae species are popularly known as sempre-vivas. This family comprising approximately 1.200 species divided into 10 genera, with high endemic levels. Paepalanthus genus has high incidence in the Espinhaço Range region and the report of biological activities. Despite the large number of previous studies with Eriocaulaceae species, Paepalanthus geniculatus has no studies about their scapes, which demonstrates the need for new research to identify their chemical and biological composition. The aim of this work was to study the chemical composition of P. geniculatus ethanolic extract seeking the isolation and identification of metabolites and evaluate the radical scavenging activity of the extract and isolated substances. P. geniculatus were collected in Serra do Cipó-MG in 2013 (Voucher: SANO 3193) and the scapes were dried, crushed and the powder was percolated with ethanol. With the etanolic extract it is carried out a fractionation by gel permeation chromatography, yielding fractions analyzed by TLC assay. The fraction J89 (45,6 mg) was purified by semipreparative HPLC-PDA, resulting in the isolation of substance S1. The fractionation was also performed by medium pressure liquid chromatography, yielding fractions analyzed by TLC assay. The fraction number 4 (92,6 mg) were purified by semipreparative HPLC-PDA resulting in the isolation of substances S2 and S3. The substances S1 and S3 were analyzed by mono and twodimensional NMR, resulting in the identification of 3,4-dihydroxybenzoic acid (protocatechuic acid) and the flavonoid 6-hydroxyquercetin-7-O-β-Dglucopyranoside. The substance S2 were identified by comparison with standards, were it was possible to determine the presence of the flavonoid 6-hydroxy-7- methoxyquercetin-3-O-β-D-glucopyranoside. The evaluation of radical scavenging activity for the extract and the isolated substances using DPPH, showed consistent activity to S1...

Paepalanthus sphaeroides, a new species of Eriocaulaceae from the Atlantic Forest, Brazil

We describe and illustrate Paepalanthus sphaeroides (Eriocaulaceae, Paepalanthoideae) from the Mantiqueira Range in South-eastern Brazil and compare it with the morphologically most similar species: Paepalanthus aequalis and Paepalanthus eriophaeus. Paepalanthus sphaeroides has unique membranaceous sheaths tightly adpressed to the scapes, patent involucral bracts slightly surpassing the capitula, and sepals of the staminate flowers fused from the base to the middle as distinctive characteristics. Comments on morphological variation, geographical distribution, ecology, conservation status, as well as a distribution map, line drawings, and photos are provided.

Ol-Prx 3, a member of an additional class of homeobox genes, is unimodally expressed in several domains of the developing and adult central nervous system of the medaka (Oryzias latipes)

Large-scale genetic screens for mutations affecting early neurogenesis of vertebrates have recently been performed with an aquarium fish, the zebrafish. Later stages of neural morphogenesis have attracted less attention in small fish species, partly because of the lack of molecular markers of developing structures that may facilitate the detection of discrete structural alterations. In this context, we report the characterization of Ol-Prx 3 (Oryzias latipes-Prx 3). This gene was isolated in the course of a large-scale screen for brain cDNAs containing a highly conserved DNA binding region, the homeobox helix-three. Sequence analysis revealed that this gene belongs to another class of homeobox genes, together with a previously isolated mouse ortholog, called OG-12 [Rovescalli, A. C., Asoh, S. & Nirenberg, M. (1996) Proc. Natl. Acad. Sci. USA 93, 10691–10696] and with the human SHOX gene [Rao, E., Weiss, B., Fukami, M., Rump, A., Niesler, B., et al. (1997) Nat. Genet. 16, 54–62], thought to be involved in the short-stature phenotype of Turner syndrome patients. These three genes exhibit a moderate level of identity in the homeobox with the other genes of the paired-related (PRX) gene family. Ol-Prx 3, as well as the PRX genes, are expressed in various cartilaginous structures of head and limbs. These genes might thus be involved in common regulatory pathways during the morphogenesis of these structures. Moreover, this paper reports a complex and monophasic pattern of Ol-Prx 3 expression in the central nervous system, which differs markedly from the patterns reported for the PRX genes, Prx 3 excluded: this gene begins to be expressed in a variety of central nervous system territories at late neurula stage. Strikingly, it remains turned on in some of the derivatives of each territory during the entire life of the fish. We hope this work will thus help identify common features for the PRX 3 family of homeobox genes.

Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: A useful model to monitor germ cells in a live vertebrate

We have generated transgenic medaka (teleost, Oryzias latipes), which allow us to monitor germ cells by green fluorescent protein (GFP) fluorescence in live specimens. Two medaka strains, himedaka (orange–red variety) and inbred QurtE, were used. The transgenic lines were achieved by microinjection of a construct containing the putative promoter region and 3′ region of the medaka vasa gene (olvas). The intensity of GFP fluorescence increases dramatically in primordial germ cells (PGCs) located in the ventrolateral region of the posterior intestine around stage 25 (the onset of blood circulation). Whole-mount in situ hybridization and monitoring of ectopically located cells by GFP fluorescence suggested that (i) the increase in zygotic olvas expression occurs after PGC specification and (ii) PGCs can maintain their cell characteristics ectopically after stages 20–25. Around the day of hatching, the QurtE strain clearly exhibits sexual dimorphisms in the number of GFP fluorescent germ cells, a finding consistent with the appearance of leucophores, a sex-specific marker of QurtE. The GFP expression persists throughout the later stages in the mature ovary and testis. Thus, these transgenic medaka represent a live vertebrate model to investigate how germ cells migrate to form sexually dimorphic gonads, as well as a potential assay system for environmental substances that may affect gonad development. The use of a transgenic construct as a selective marker to efficiently isolate germ-line-transmitting founders during embryogenesis is also discussed.

Silver toxicity across salinity gradients: the role of dissolved silver chloride species (AgCl x ) in Atlantic killifish (Fundulus heteroclitus) and medaka (Oryzias latipes) early life-stage toxicity.

The influence of salinity on Ag toxicity was investigated in Atlantic killifish (Fundulus heteroclitus) early life-stages. Embryo mortality was significantly reduced as salinity increased and Ag(+) was converted to AgCl(solid). However, as salinity continued to rise (>5 ‰), toxicity increased to a level at least as high as observed for Ag(+) in deionized water. Rather than correlating with Ag(+), Fundulus embryo toxicity was better explained (R(2) = 0.96) by total dissolved Ag (Ag(+), AgCl2 (-), AgCl3 (2-), AgCl4 (3-)). Complementary experiments were conducted with medaka (Oryzias latipes) embryos to determine if this pattern was consistent among evolutionarily divergent euryhaline species. Contrary to Fundulus data, medaka toxicity data were best explained by Ag(+) concentrations (R(2) = 0.94), suggesting that differing ionoregulatory physiology may drive observed differences. Fundulus larvae were also tested, and toxicity did increase at higher salinities, but did not track predicted silver speciation. Alternatively, toxicity began to increase only at salinities above the isosmotic point, suggesting that shifts in osmoregulatory strategy at higher salinities might be an important factor. Na(+) dysregulation was confirmed as the mechanism of toxicity in Ag-exposed Fundulus larvae at both low and high salinities. While Ag uptake was highest at low salinities for both Fundulus embryos and larvae, uptake was not predictive of toxicity.

Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos.

This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001-10 µM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from stage 10 (6-7 hpf) to 10 days post fertilization (dpf). Of the forms of mercury in this study, the organic form (MeHg) proved the most toxic followed by inorganic mercury (HgCl2), both producing embryo developmental toxicity. Altered phenotypes included pericardial edema with elongated or tube heart, reduction of eye pigmentation, and failure of swim bladder inflation. Both α-HgS and β-HgS were less toxic than MeHg and HgCl2. Total RNA was extracted from survivors three days after exposure to MeHg (0.1 µM), HgCl2 (1 µM), α-HgS (10 µM), or β-HgS (10 µM) to examine toxicity-related gene expression. MeHg and HgCl2 markedly induced metallothionein (MT) and heme oxygenase-1 (Ho-1), while α-HgS and β-HgS failed to induce either gene. Chemical forms of mercury compounds proved to be a major determinant in their developmental toxicity.

Manejo de las tres principales plagas del sorgo (Sorghum bicolor L. Moench), gusano cogollero (Spodoptera frugiperda), mosquita del sorgo (Stenodiplosis sorghicola) y chinche pata de hoja (Leptoglossus zonatus) en época de postrera en la zona de ranchería, Chinandega 2003

El presente trabajo se realizó en la finca Santa Isabel ubicada en el municipio de Ranchería en el Km.20 carretera Chinandega–Somotillo en el departamento de Chinandega. Con el objetivo de evaluar el efecto de cuatro tratamientos sobre las poblaciones de Gusano Cogollero Spodoptera frugiperda (J. E. Smith), Mosquita del sorgo Stenodiplosis sorghicola (Coquillet) y Chinche pata de hoja Leptoglossus zonatus (Dallas), durante la época de postrera en los meses de Septiembre a Diciembre del 2003. Los tratamientos evaluados fueron Barrera viva de gandul-sorgo, Nim aceite, Beauveria bassiana en polvo y Diazinón, comparados con una parcela sin aplicación (testigo). Se utilizaron umbrales de 40 % de daño para el cogollero, dos individuos por panoja para la mosquita del sorgo y un individuo por panoja para el chinche pata de hoja, los muestreos se realizaron cada semana, iniciando desde la siembra hasta la cosecha. Se seleccionaron cinco sitios al azar en cada tratamiento y se registro el número de plagas observadas. Se realizaron dos aplicaciones, una primera aplicación en etapa vegetativa para los desfoliadores y una segunda aplicación en etapa reproductiva cuando mosquita del sorgo alcanzo el umbral establecido. No se encontró diferencia estadística entre los tratamientos, sin embargo en el tratamiento Nim aceite se presentaron menor número de plagas. Los mayores rendimiento se presentaron en el tratamiento Nim aceite y en el tratamiento Barrera viva (gandul-sorgo) con 2,370.6 Kg. /Ha y 2,234.7 Kg./Ha respectivamente. El análisis económico indica que el tratamiento Barrera viva (gandul- sorgo) presentó mayor rentabilidad con 170.97%. La especie de insecto plaga predominante en la zona de Ranchería, Chinandega durante la época de estudio fue Mocis latipes (gusano medidor) así mismo se presento la enfermedad conocida como moho del grano causada por un complejo de hongos Fusarium moniliforme sheldon y Curvularia lunata. En época de postrera 2003

Two genera and species of Isopoda new to the fauna of the northern Arabian Sea

The paper is based on the new records of two genera - Lanocira Hansen and Paranthura Bate and Westwood and species L. gardineri Stebbing and P. latipes Barnard from the rocky intertidal zone of Karachi coast. Synonymes, diagnoses and geographical distribution of the genera are given. A list of known species of the genus Lanocira is provided. Both the species are described and illustrated in detail.

云南青jiang鱼类的分类和分布(jiang形目:青jiang科)

记录了采自云南的青jiang属1新亚种及1新纪录种。中华青jiang, 新亚种O. latipes sinensis subsp. nov.与日本青jiang指名亚种O. latipes的主要区别: 新亚种第一肋骨连接第二脊椎骨上, 平均脊椎数为29.2±0.6, 胸鳍条多数为9, 染色体为2n=46。小青jiangO. minutillus新纪录种, 分布于西双版纳。还报导了青jiang属的地理分布, 介绍了属的特征。新种模式标本存中国科学院昆明动物研究所鱼类标本室。图3表2参5

鲤科鱼类结缔组织生长因子基因变异和分子进化

结缔组织生长因子(CTGF)在细胞生长、分化和凋亡及个体生长发育中具有重要的调控作用.研究CTGF基因在不同鱼类中的变异及进化,对于理解物种分化和基因差异具有重要意义.文中通过PCR扩增、克隆和测序,获得了19种鲤科鱼类(Cyprinidae)CTGF基因序列,并重建了鲤科鱼类系统发育关系,用于估计各分支的进化速率.以鳉科(Cyprinodontidae)的青鳉(Oryzias latipes)作为外类群,系统树均以较高的节点支持率支持鲤科鱼类中雅罗鱼系(Leuciscini)和鲃系(Barbini)的划

Medaka vasa is required for migration but not survival of primordial germ cells

Vasa is essential for germline development. However, the precise processes in which vasa involves vary considerably in diverse animal phyla. Here we show that vasa is required for primordial germ cell (PGC) migration in the medakafish. vasa knockdown by two morpholinos led to the PGC migration defect that was rescued by coinjection of Vasa RNA. Interestingly, Vasa knockdown did not alter the PGC number, identity, proliferation and motility even at ectopic locations. We established a cell culture system for tracing PGCs at the single cell level in vitro. In this culture system, control and morpholino-injected gastrulae produced the same PGC number and the same time course of PGC survival. importantly, vasa-depleted PGCs in culture had similar motility and locomotion to normal PGCs. Expression patterns of wt1a, sdf1b and cxcT4b in migratory tissues remained unchanged by Vasa knockdown. By chimera formation we show that PGCs from vasa-depleted blastulae failed to migrate properly in the normal environment, whereas control PGCs migrated normally in vasa-disrupted embryos. Furthermore, ectopic PGCs in vasa-depleted embryos also retained all the PGC properties examined. Taken together, medaka vasa is cell-autonomously required for PGC migration, but dispensable to PGC proliferation, motility, identity and survival. (C) 2009 Elsevier Ireland Ltd. All rights reserved.