Treatment of experimental autoimmune encephalomyelitis by feeding myelin basic protein conjugated to cholera toxin B subunit.

Oral administration of autoantigens can prevent and partially suppress autoimmune diseases in a number of experimental models, Depending on the dose of antigen fed, this approach appears to involve distinct yet reversible and short-lasting mechanisms (anergy/deletion and suppression) and usually requires repeated feeding of large (suppression) to massive (anergy/deletion) amounts of autoantigens to be effective. Most importantly, this approach is relatively less effective in animals already systemically sensitized to the fed antigen, such as in animals already harboring autoreactive T cells and, thus, presumably also in humans suffering from an autoimmune disorder. We have previously shown that feeding a single dose of minute amounts of antigens conjugated to cholera toxin B subunit (CTB) can effectively suppress delayed-type hypersensitivity reactions in systemically immune animals. We now report that feeding small amounts of myelin basic protein (MBP) conjugated to CTB either before or after disease induction protected rats from experimental autoimmune encephalomyelitis. Such treatment was as effective in suppressing interleukin 2 production and proliferative responses of lymph node cells to MBP as treatment involving repeated feeding with much larger (50- to 100-fold) doses of free MBP. Different from the latter treatment, which led to decreased production of interferon-gamma in lymph nodes, low-dose oral CTB-MBP treatment was associated with increased interferon-gamma production. Most importantly, low-dose oral CTB-MBP treatment greatly reduced the level of leukocyte infiltration into spinal cord tissue compared with treatment with repeated feeding of large doses of MBP. These results suggest that the protection from experimental autoimmune encephalomyelitis achieved by feeding CTB-conjugated myelin autoantigen involves immunomodulating mechanisms that are distinct from those implicated by conventional protocols of oral tolerance induction.

Transcytosis of cholera toxin subunits across model human intestinal epithelia.

Cholera toxin (CT) elicits a massive secretory response from intestinal epithelia by binding apical receptors (ganglioside GM1) and ultimately activating basolateral effectors (adenylate cyclase). The mechanism of signal transduction from apical to basolateral membrane, however, remains undefined. We have previously shown that CT action on the polarized human intestinal epithelial cell line T84 requires endocytosis and processing in multiple intracellular compartments. Our aim in the present study was to test the hypothesis that CT may actually move to its site of action on the basolateral membrane by vesicular traffic. After binding apical receptors, CT entered basolaterally directed transcytotic vesicles. Both CT B subunits and to a lesser extent CT A subunits were delivered intact to the serosal surface of the basolateral membrane. The toxin did not traverse the monolayer by diffusion through intercellular junctions. Transcytosis of CT B subunits displayed nearly identical time course and temperature dependency with that of CT-induced Cl- secretion--suggesting the two may be related. These data identify a mechanism that may explain the link between the toxin's apical receptor and basolateral effector.

Saxton's rural hand books. containing The Horse, The Hog, The Honey bee, The Pests of the farm, Domestic fows, The Cow.

Horses; their varieties, breeding, and management in health and disease -- The hog; his originand varieties : management with a view to profit, and treatment under disease -- The hive and the honey-bee; with plain directions for obtaining a considerable annual income from this branch of rural economy -- Domestic fowl and ornamental poultry; their natural history, origin, and treatment in health and disease -- The cow: dairy husbandry and cattle breeding / by M. M. Milburn.

Home pork making : a complete guide for the farmer, the country butcher and the suburban dweller, in all that pertains to hog slaughtering, curing, preserving and storing pork product /

Includes index.

More profit from hogs : information on the advantages of insulating hog houses and illustrations showing the various breeds of profit-making hogs.

Mode of access: Internet.

A great convention of drunkards held in the state of Agitation, in the county of Spking[!]Water near Total-tee, the county seat : Also a great hog convention held in the state of Cool Reflection soon after the burning of the great destillery at Oak Orchard.

Mode of access: Internet.

Discours et aduis sur les flus de ventre douloureux, soit qu'il yait du sang ou point, sur le trousse-gallant, dict cholera morbus ... ; Avec addition d'un appendice de la goutte, & de la sciatique en particulier ... /

Mode of access: Internet.

Cholera-morbus /

Tesis Univ. Río de Janeiro.

Do cholera-murbus : etymologia, definiçâo e historico /

Tesis Univ. Río de Janeiro.

Instructions sanitaires sur les moyens préservatifs du cholera-morbus /

Mode of access: Internet.

Recherches sur la conduite à tenir dans le traitement du cholera algide ou asiatique /

Mode of access: Internet.

Papers relative to the disease called cholera spasmodica in India : now prevailing in the north of Europe /

Mode of access: Internet.

Apuntes sobre el cholera-morbus, y su curacion con la mikania-huaco o guaco /

Mode of access: Internet.

Sketches of the most prevalent diseases of India : comprising, a treatise on the epidemic cholera of the East, statistical and topographical reports of the diseases in the different divisions of the army under the Madras presidency ... : iullustrated by tables and plates /

Mode of access: Internet.