Avaliação in vitro da adesão e proliferação de células mesenquimais do ligamento periodontal humano em diferentes superfícies de titânio

In the last years, many scientific researches in implantology have been focused on alternatives that would provide higher speed and quality in the process of osseointegration. Different treatment methods can be used to modify the topographic and chemical properties of titanium surface in order to optimize the tissue-implant reactions by a positive tissue response. This study aimed to evaluate the adhesion and proliferation of mesenchymal cells from human periodontal ligament on two different titanium surfaces, using cell culture techniques. Grade II titanium discs received different surface treatments, forming two distinct groups: polished and cathodic cage plasma nitriding. Human periodontal ligament mesenchymal cells were cultured on titanium discs in 24-well cell culture plates, at a density of 2 x 104 cells per well, including wells with no discs as positive control. Data obtained by counting the cells that adhered to the titanium surfaces (polished group and cathodic cage group) and to the plastic surface (control group), in the 24, 48 and 72-hour periods after plating, were used to analyze cell adhesion and proliferation and to obtain the cell growing curve in the different groups. The data were submitted to nonparametric analysis and the differences between groups were compared by Kruskal-Wallis and Friedman statistical tests. No statistically significant differences were found in the cells counts between the groups (p>0.05). It was concluded that both treatments produced surfaces compatible with the adhesion and proliferation of human periodontal ligament mesenchymal cells

Estudo comportamental, hormonal e histopatológico de animais com perda de peso progressina (SEP) e da variável peso associada a uma nova classificação ontogenética em Callithrix jacchus

The use of non-human primates in scientific research has contributed significantly to the biomedical area and, in the case of Callithrix jacchus, has provided important evidence on physiological mechanisms that help explain its biology, making the species a valuable experimental model in different pathologies. However, raising non-human primates in captivity for long periods of time is accompanied by behavioral disorders and chronic diseases, as well as progressive weight loss in most of the animals. The Primatology Center of the Universidade Federal do Rio Grande do Norte (UFRN) has housed a colony of C. jacchus for nearly 30 years and during this period these animals have been weighed systematically to detect possible alterations in their clinical conditions. This procedure has generated a volume of data on the weight of animals at different age ranges. These data are of great importance in the study of this variable from different perspectives. Accordingly, this paper presents three studies using weight data collected over 15 years (1985-2000) as a way of verifying the health status and development of the animals. The first study produced the first article, which describes the histopathological findings of animals with probable diagnosis of permanent wasting marmoset syndrome (WMS). All the animals were carriers of trematode parasites (Platynosomum spp) and had obstruction in the hepatobiliary system; it is suggested that this agent is one of the etiological factors of the syndrome. In the second article, the analysis focused on comparing environmental profile and cortisol levels between the animals with normal weight curve evolution and those with WMS. We observed a marked decrease in locomotion, increased use of lower cage extracts and hypocortisolemia. The latter is likely associated to an adaptation of the mechanisms that make up the hypothalamus-hypophysis-adrenal axis, as observed in other mammals under conditions of chronic malnutrition. Finally, in the third study, the animals with weight alterations were excluded from the sample and, using computational tools (K-means and SOM) in a non-supervised way, we suggest found new ontogenetic development classes for C. jacchus. These were redimensioned from five to eight classes: infant I, infant II, infant III, juvenile I, juvenile II, sub-adult, young adult and elderly adult, in order to provide a more suitable classification for more detailed studies that require better control over the animal development

As competições sociais em grupos de Callithrix jacchus (Callitrichidae, Primates) são influenciadas pelo parentesco e pela convivência?

The Callitrichidae family is characterized by flexibility in its mating system, being possible to find monogamous, polyandrid and polygynic groups. Its social organization and mating system can be defined by the interaction between ecological and demographic factors plus the degree of relatedness among the individuals in the population. The objective of this work was to demonstrate the influence of relatedness and coexistence on the establishment and maintenance of social relations between Callithrix jacchus adult males. Four pairs of related adult males (CP), 4 pairs with coexistence between the animals in the pair (CC) and 4 pairs with no relatedness (SP) were studied. The pairs in the group CC had been kept in the same cage for at least 8 months before the experiment and the pairs in the group SP were put together at the beginning of the study. Each animal was observed 3 times/week for 2 months, in 15 min. sessions, through focal time sampling with instantaneous record each minute. In the first month, only the pair of males was kept in the cage (Phase I) and in the second month, a female was introduced into the cage (Phase II). The affiliative, agonistic and sexual interactions were registered. Affiliative interactions showed similar frequencies for all groups in phases. There was also no significant difference in the agonistic interactions of the CP, CC and SP males in Pase I, even considering that group SP exhibited higher levels of agonism. In Phase II, there was a statistically significant increase comparing to the others. The results demonstrate that relatedness and coexistence are of great importance for maintaining reprodutive and social stability inside the group. Nonetheless, in this study, only one of the males in the pair had sexual interations with the females, except for 2 pairs. This was assured through mate guarding and agonism directed to the potencial competitor. The most probable mating system would be functional monogamy, where the males would present low levels of competition, if there is relatedness and coexistence among them; on the other hand, a strong competition if there is no relatedness between the individuals. Even so, a polyandric system would also have to be considered

Relações sociais de callithrix jacchus machos: competição ou cooperação?

The group Callithrichidae is characterized by the flexibility in its mating system. This fact generates questions about the mechanisms that Callithrix jacchus males reach the breeding position in a group and how they prevent the subordinates reproduction, since the competition apparently happens with less intensity when compared to the females. Studies have shown the importance of social interactions and kinship among individuals in the distribution of mating opportunities in a group. This work aimed to evaluate if dyads of Callithrix jacchus males acted socially different due to kinship. We observed the agonistic and affiliative behaviours made by the males when associated in dyads (Phase 1) and when these dyads received a female (Phase 2), which was introduced in the pair`s cage for 45 minutes. In the phase 2, we also accounted for the sexual interactions between males and female in each trio. We used 16 males, 8 related (brothers-R) and 8 nonrelated (NR), as well as 8 females. Each phase lasted 4 weeks, with 3 observations/week, during 15 min/male in phase 1 and 2. In the first phase, two pairs of R males had a contested dominance and the other two, just like in the NR males, had an uncontested dominance. The NR dominant males were more aggressive than the subordinates. With the female`s entrance in the cage, all male pairs began to have an uncontested dominance. The NR males aggression frequency rose along with the subordinates` submission. In the R males, the frequency of grooming and physical contact was higher than in the NR males. So, we conclude that the low levels of agonism presented by the R males, along with the higher tolerance shown amongst them, lead us to believe that kinship attenuate the competition among these males

Índice de aromaticidade baseado na deslocalização, degenerescência e densidade (D3BIA) para acenos e moléculas homoaromáticas

The aromaticity index is an important tool for the investigation of aromatic molecules. This work consists on new applications of the aromaticity index developed by teacher Caio Lima Firme, so-called D3BIA (density, delocalization, degeneracy-based index of aromaticity). It was investigated its correlation with other well-known aromaticity indexes, such as HOMA (harmonic oscillator model of aromaticity), NICS (nucleus independent chemical shielding), PDI (para-delocalization index), magnetic susceptibility (), and energetic factor in the study of aromaticity of acenes and homoaromatic species based on bisnoradamantanyl cage. The density functional theory (DFT) was used for optimization calculations and for obtaining energetic factors associated with aromaticity and indexes HOMA and NICS. From quantum theory of atoms in molecules (QTAIM) it was obtained the indexes D3BIA, PDI and . For acenes, when the over-mentioned indexes were applied it was observed no correlation except for D3BIA and HOMA (R2=0.752). For bisnoradamantenyl dication and its derivatives, it was obtained a good correlation between D3BIA and NICS. Moreover, it was evaluated solely one of the factors used on D3BIA calculation, the delocalization index uniformity (DIU), so as to investigate its possible influence on stability of chemical species. Then, the DIU was compared with the formation Gibbs free energy of some pairs of carbocations, isomers or not, which each pair had small difference in point group symmetry and no difference among other well-known stability factors. The obtained results indicate that DIU is a new stability factor related to carbocations, that is, the more uniform the electron density delocalization, the more stable the is carbocation. The results of this work validate D3BIA and show its importance on the concept of aromaticity, indicating that it can be understood from degeneracy of atoms belonging the aromatic site, the electronic density in the aromatic site and the degree of uniformity of electron delocalization

Toward reliable estimates of seed removal by small mammals and birds in the Neotropics

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Demographic aspects of Chrysomya megacephala (Diptera, Calliphoridae) adults maintained under experimental conditions: reproductive rate estimates

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Método experimental para determinação das capacitancias parasitas do motor de indução trifásico acionado por inversor MLP

A proposta deste trabalho é apresentar uma nova metodologia para determinação experimental das capacitancias parasitas do motor de indução trifásico de rotor em gaiola. As capacitancias parasitas fazem parte do circuito equivalente do motor para estudos de interferência eletromagnética causada no motor de indução em modo comum quando ele for acionado por inversor controlado por modulação por largura de pulsos (MLP). Os procedimentos propostos para o desenvolvimento deste novo método consistem em: a) determinação dos parâmetros do circuito equivalente do motor de indução trifásico, em regime permanente, através de ensaio em laboratório; b) estabelecer configurações de ligações entre o inversor MLP e o motor para medições das grandezas de interesse que são as seguintes: tensões de modo comum e de eixo, correntes de fuga e de eixo, através de circuito de medição desenvolvido para este fim; c) calcular os valores das capacitancias parasitas entre estator e carcaça do motor; estator e rotor; rotor e carcaça e de rolamento utilizando a expressão matemática da definição de capacitancia; d) utilizar o software Pspice para simular o sistema motor de indução trifásico, alimentado por inversor MLP, com os circuitos equivalentes em baixas e altas frequências; e) obter as formas de onda características do fenômeno de modo comum.

Temperatura e enriquecimento ambiental sobre o bem-estar de coelhos em crescimento

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produtividade de ratos Wistar em diferentes sistemas de acasalamento

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Handling of female pacas (Agouti paca, Linnaeus, 1766) for ultrasound pregnancy detection

A paca é um roedor sul-americano cujo manejo, tranqüilização ou anestesia oferece um grande campo de pesquisa devido aos poucos artigos existentes. Doze pacas fêmeas adultas foram utilizadas, mantidas separadas em seis baias, sendo capturadas com um puçá de polipropileno e levadas a uma sala na qual se realizava a tricotomia abdominal. Após, os animais eram colocados em uma gaiola de ferro de compressão lateral. A sessão de ultra-sonografia era realizada em modo B com um transdutor setorial eletrônico de 5,0 e 7,5 MHz. Para a diminuição do estresse causado pelos procedimentos, a tranqüilização das fêmeas era realizada com diazepam e maleato de midazolam por via oral. Ambos demonstraram-se efetivos na tranqüilização das pacas previamente e durante as sessões, sendo que o maleato de midazolam proporcionou um melhor manejo dos animais.

Modelagem de um motor de indução trifásico operando com tensões desequilibradas por meio de redes neurais artificiais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da mobilização precose e da mobilizaçao sobre o reparo do tendão patelar em ratos: análise morfológica e morfométrica

Pós-graduação em Biologia Geral e Aplicada - IBB

Efeitos do atipamezol e ioimbina na recuperação da anestesia com dexmedetomidina e cetamina, em papagaios verdadeiros (Amazona aestiva)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Eficácia da soja geneticamente modificada MON 87701 × MON 89788 com a expressão da proteína Cry1Ac no controle de Anticarsia gemmatalis (Hübner) e Pseudoplusia includens (Walker) (Lepidoptera: Noctuidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)