Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building

The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.

Subordinação discursiva no português à luz da gramática discursivo-funcional

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da prática de escritores contemporâneos guineenses

The aim of this final essay is to study the writing practice of contemporary writers from Guinea Bissau, one of the countries of the former Portuguese colony, where the literature has developed very late, due to the lack of socio-cultural conditions for the development of the this practice. This happened because the country was delay was under colonial exploitation for a long time, under the command of the portuguese government. Among the elements that explain this situation is the presence of a restrictive and longcolonial education policy.The interest of this research consists in issues relating to the practice of writing and identity, It is an exploratory study which aims to gather information and reflect a) on discourses of contemporary Guinean writers about their writing practice and b) journalistic and academic discourses about the writing practice of these writers. The search of the corpus is made mainly on the Internet and the time frame set for the talks is collected from 1990 to 2010. The issues that guide the research are: 1) How is the writing practice of these writers ?, 2) What is the purpose of writing in this socio-historical context?, 3) What are the consequences of this practice for the production of the identity of these individuals? The results have revealed that: the practice of writing have a important role in the production of Guineansubject identity, because it has been related to the identity and political movements; the use of native languages and Creole in these practices, is indicative of the attempt to build in writing, an identity for the self who writes and for the other represented. This identity speaks to the cultural manifestations of the African people

A relação conclusiva na língua portuguesa: funções resumo, conclusão e consequência

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Brasil, Moçambique e Angola: desvendando relações sociolinguísticas pelo prisma das formas de tratamento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Práticas culturais no ensino de português para estrangeiros

The article entitled Cultural Practices in Teaching Portuguese for Foreigners aims to disseminate teaching practices facing the language teaching to students from Guinea Bissau or whom the Portuguese of Portugal is the official language. We seek to problematize the course given to those students with the purpose of appropriating the " resources of expression" for approval in subjects who were enrolled in Brazil in the first half of undergraduate Letras course in a higher education institution.

The phylogeography of trypanosomes from South American alligatorids and African crocodilids is consistent with the geological history of South American river basins and the transoceanic dispersal of Crocodylus at the Miocene

Abstract Background Little is known about the diversity, phylogenetic relationships, and biogeography of trypanosomes infecting non-mammalian hosts. In this study, we investigated the influence of host species and biogeography on shaping the genetic diversity, phylogenetic relationship, and distribution of trypanosomes from South American alligatorids and African crocodilids. Methods Small Subunit rRNA (SSU rRNA) and glycosomal Glyceraldehyde Phosphate Dehydrogenase (gGAPDH) genes were employed for phylogenetic inferences. Trypanosomes from crocodilians were obtained by haemoculturing. Growth behaviour, morphology, and ultrastructural features complement the molecular description of two new species strongly supported by phylogenetic analyses. Results The inferred phylogenies disclosed a strongly supported crocodilian-restricted clade comprising three subclades. The subclade T. grayi comprised the African Trypanosoma grayi from Crocodylus niloticus and tsetse flies. The subclade T. ralphi comprised alligatorid trypanosomes represented by Trypanosoma ralphi n. sp. from Melanosuchus niger, Caiman crocodilus and Caiman yacare from Brazilian river basins. T. grayi and T. ralphi were sister subclades. The basal subclade T. terena comprised alligatorid trypanosomes represented by Trypanosoma terena n. sp. from Ca. yacare sharing hosts and basins with the distantly genetic related T. ralphi. This subclade also included the trypanosome from Ca. crocodilus from the Orinoco basin in Venezuela and, unexpectedly, a trypanosome from the African crocodilian Osteolaemus tetraspis. Conclusion The close relationship between South American and African trypanosomes is consistent with paleontological evidence of recent transoceanic dispersal of Crocodylus at the Miocene/Pliocene boundaries (4–5 mya), and host-switching of trypanosomes throughout the geological configuration of South American hydrographical basins shaping the evolutionary histories of the crocodilians and their trypanosomes.

The phylogeography of trypanosomes from South American alligatorids and African crocodilids is consistent with the geological history of South American river basins and the transoceanic dispersal of Crocodylus at the Miocene

Background: Little is known about the diversity, phylogenetic relationships, and biogeography of trypanosomes infecting non-mammalian hosts. In this study, we investigated the influence of host species and biogeography on shaping the genetic diversity, phylogenetic relationship, and distribution of trypanosomes from South American alligatorids and African crocodilids. Methods: Small Subunit rRNA (SSU rRNA) and glycosomal Glyceraldehyde Phosphate Dehydrogenase (gGAPDH) genes were employed for phylogenetic inferences. Trypanosomes from crocodilians were obtained by haemoculturing. Growth behaviour, morphology, and ultrastructural features complement the molecular description of two new species strongly supported by phylogenetic analyses. Results: The inferred phylogenies disclosed a strongly supported crocodilian-restricted clade comprising three subclades. The subclade T. grayi comprised the African Trypanosoma grayi from Crocodylus niloticus and tsetse flies. The subclade T. ralphi comprised alligatorid trypanosomes represented by Trypanosoma ralphi n. sp. From Melanosuchus niger, Caiman crocodilus and Caiman yacare from Brazilian river basins. T. grayi and T. ralphi were sister subclades. The basal subclade T. terena comprised alligatorid trypanosomes represented by Trypanosoma terena n. sp. from Ca. yacare sharing hosts and basins with the distantly genetic related T. ralphi. This subclade also included the trypanosome from Ca. crocodilus from the Orinoco basin in Venezuela and, unexpectedly, a trypanosome from the African crocodilian Osteolaemus tetraspis. Conclusion: The close relationship between South American and African trypanosomes is consistent with paleontological evidence of recent transoceanic dispersal of Crocodylus at the Miocene/Pliocene boundaries (4–5 mya), and host-switching of trypanosomes throughout the geological configuration of South American hydrographical basins shaping the evolutionary histories of the crocodilians and their trypanosomes.

Culture's Calling: Mobile Phones, Gender, and the Making of an African Migrant Village in Lisbon

In this article, I explore how immigrants from the West African nation of Guinea-Bissau living in Portugal use mobile phones in their daily lives in Lisbon. Whereas one might assume that mobile phones and other new information technologies facilitate transnational communication between Africa and Portugal, the ethnographic fieldwork that I conducted in Lisbon from 1999 to 2003 revealed a different scenario. Instead, mobile phones as imagined and used by the Guinean immigrants I met in Lisbon revealed less about transnationalism and globalization than they did about constructing community and identity in a new locale. As Guinean immigrants in Portugal reconfigured their relationship to their former colonizers and struggled to make their way in a new, multicultural Europe, they used their mobile phones to engage local networks, shape local identities, and transform Lisbon's sprawl into an African migrant village. Here, I highlight the gendered dimensions of this process and contend that Guinean men's and women's varied uses of mobile phones in Lisbon underscore contrasting experiences of migration, mobility, and belonging.

Culture's Calling: Mobile Phones, Gender, and the Making of an African Migrant Village in Lisbon

In this article, I explore how immigrants from the West African nation of Guinea-Bissau living in Portugal use mobile phones in their daily lives in Lisbon. Whereas one might assume that mobile phones and other new information technologies facilitate transnational communication between Africa and Portugal, the ethnographic fieldwork that I conducted in Lisbon from 1999 to 2003 revealed a different scenario. Instead, mobile phones as imagined and used by the Guinean immigrants I met in Lisbon revealed less about transnationalism and globalization than they did about constructing community and identity in a new locale. As Guinean immigrants in Portugal reconfigured their relationship to their former colonizers and struggled to make their way in a new, multicultural Europe, they used their mobile phones to engage local networks, shape local identities, and transform Lisbon's sprawl into an African migrant village. Here, I highlight the gendered dimensions of this process and contend that Guinean men's and women's varied uses of mobile phones in Lisbon underscore contrasting experiences of migration, mobility, and belonging.

Effectiveness of improved cookstoves to reduce indoor air pollution in developing countries. The case of the cassamance natural subregion, Western Africa

The Spanish NGO "Alianza por la Solidaridad" has installed improved cookstoves in 3000 households during 2012 and 2013 to improve energy efficiency reducing fuelwood consumption and to improve in-door air quality. The type of cookstoves were Noflaye Jeeg and Noflaye Jaboot and were installed in the Cassamance Natural Subregion covering part of Senegal, The Gambia and Guinea-Bissau. The Technical University of Madrid (UPM) has conducted a field study on a sample of these households to assess the effect of improved cookstoves on kitchen air quality. Measurements of carbon monoxide (CO) and fine particle matter (PM2.5) were taken for 24-hr period before and after the installation of improved cook-stoves. The 24-hr mean CO concentrations were lower than the World Health Organization (WHO) guidelines for Guinea-Bissau but higher for Senegal and Gambia, even after the installation of improved cookstoves. As for PM2.5 concentrations, 24-hr mean were always higher than these guidelines. However, improved cookstoves produced significant reductions on 24-hr mean CO and PM2.5 concentrations in Senegal and for mean and maximum PM2.5 concentration on Gambia. Although this variability needs to be explained by further research to determine which other factors could affect indoor air pollution, the study provided a better understanding of the problem and envisaged alternatives to be implemented in fu-ture phases of the NGO project.

Effectiveness of improved cookstoves to reduce indoor air pollution in developing countries. The case of the Cassamance Natural Subregion, Western Africa

The Spanish NGO "Alianza por la Solidaridad" has installed improved cookstoves in 3000 households during 2012 and 2013 to improve energy efficiency reducing fuelwood consumption and to improve indoor air quality. The type of cookstoves were Noflaye Jeeg and Noflaye Jaboot and were installed in the Cassamance Natural Subregion covering part of Senegal, The Gambia and Guinea-Bissau. The Technical University of Madrid (UPM) has conducted a field study on a sample of these households to assess the effect of improved cookstoves on kitchen air quality. Measurements of carbon monoxide (CO) and fine particle matter (PM2.5) were taken for 24-hr period before and after the installation of improved cookstoves. The 24-hr mean CO concentrations were lower than the World Health Organization (WHO) guidelines for Guinea-Bissau but higher for Senegal and Gambia, even after the installation of improved cookstoves. As for PM2.5 concentrations, 24-hr mean were always higher than these guidelines. However, improved cookstoves produced significant reductions on 24-hr mean CO and PM2.5 concentrations in Senegal and for mean and maximum PM2.5 concentration on Gambia. Although this variability needs to be explained by further research to determine which other factors could affect indoor air pollution, the study provided a better understanding of the problem and envisaged alternatives to be implemented in future phases of the NGO project.

West & North Africa, ca. 1710 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carte de la Barbarie, le [sic] la Nigritie et del la Guinée, par Guillaume de l'Isle de l'Academie Royale des Sciences. It was published by ex officina Nicolai Visscher, ca. 1710. Scale [ca.1:9,000,000]. Covers West and North Africa. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Africa Sinusoidal projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, routes and roads, territorial boundaries, shoreline features, and more. Relief shown pictorially. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

West & North Africa, ca. 1730 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carte de la Barbarie, le la Nigritie et de la Guinée, par Guill[au]me Del'Isle, de l'Academie Royale des Sciences. It was published by Chez Jean Cóvens et Corneille Mortier, Geographes ca. 1730. Scale [ca.1:9,250,000]. Covers West and North Africa. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Africa Sinusoidal projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, roads and routes, territorial boundaries, shoreline features, and more. Relief shown pictorially. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.