Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building


Autoria(s): David, S.; Sutre, A. F.; Sanca, A.; Mane, A.; Henriques, V.; Portugal, C.; Sancho, L.; Cardoso, A.; Paixao, E.; Duarte, E. L.; Leite, Clarice Queico Fujimura; Salem, J. I.; Antunes, A.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/11/2012

Resumo

The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.

Formato

1535-1537

Identificador

http://dx.doi.org/10.5588/ijtld.11.0658

International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.

1027-3719

http://hdl.handle.net/11449/42034

10.5588/ijtld.11.0658

WOS:000310173900020

Idioma(s)

eng

Publicador

Int Union Against Tuberculosis Lung Disease (i U A T L D)

Relação

International Journal of Tuberculosis and Lung Disease

Direitos

openAccess

Palavras-Chave #Mycobacterium tuberculosis #rpoB #sputum smear microscopy #sodium hypochlorite #denaturing high-performance liquid chromatography (DHPLC) sequencing
Tipo

info:eu-repo/semantics/article