Análise proteômica dos alérgenos imunodominantes do veneno da vespa social Polybia paulista

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Intraoral carcinosarcoma on the floor of the mouth mimicking a benign lesion

Carcinosarcoma is a rare malignant disease with aggressive behaviour rarely producing oral manifestations. This article reports a case of an intraoral carcinosarcoma affecting a 71-year-old black male; the diagnosis was made by histopathological and immunohistochemical analyses. Computed tomography scanning showed metastatic masses in the lungs. The patient was underwent a chemotherapy protocol regimen, but died as a consequence of the disease within 10 months of diagnosis. Distinctive characteristics of this presentation were the location of the lesion (floor of the mouth) and its clinical features resembling a benign lesion. A brief review of intraoral carcinosarcoma cases in the literature is also presented.

Coping personality type and environmental enrichment affect aggression at weaning in pigs

This study investigated the effects of different environmental treatments and personality types on aggression at mixing of newly weaned domestic piglets. From birth to weaning, 16 litters were housed with their dams in either barren (B) or larger, substrate-enriched (E) environments. At 15 days old, piglets were classified as 'high' (HR) or low resistant' (LR) in a manual restraint test (backtest), which is thought to identify proactive (HR) and reactive (LR) stress coping strategies that may reflect different personality types. At 30 days old, 128 piglets were weaned, relocated and mixed into 32 pens comprising two HR and two LR unfamiliar pigs, balanced for sex and weaning weight. Eight B and eight E groups changed environmental condition whereas the others remained in the same type of environment. Number and duration of fights. fight outcomes and unilateral fighting were scored for 5 h post-mixing and skin lesions were counted before and 5 h, 1 day and 2 days after mixing. On the day following weaning, fighting and also exploratory and oral manipulative behaviours were measured for 6 h. Generalized Linear Mixed Model analyses suggested interactions between pre-weaning environment, post-weaning environment and personality type. Overall, pre-weaning E pigs had longer fights at weaning and mixing (P=0.01) and fought for longer on the next day (P=0.02) than pre-weaning B pigs, and inflicted more skin lesions (P=0.02). Post-weaning enrichment did not affect fighting at mixing but reduced the time spent fighting the next day (P=0.03). Personality had subtle and environment-dependent effects on fighting, and influenced the "structure" rather than the amount of aggressive behaviour. HR pigs, for instance, bullied (i.e. chased surrendering pigs) more often (P=0.009) and their fighting behaviour was less affected by their relative body weight than that of LR pigs. Post-weaning E pigs showed relatively higher levels of exploratory behaviour (P=0.02) and less oral manipulative behaviour (P=0.04) than post-weaning B pigs. In particular, switching from a good quality environment (E) to a worse quality one (B) at weaning decreased exploratory behaviour on the next day, especially for LR pigs, who also tended to fight with and orally manipulate their pen mates more in that condition, and seemed to be more affected by a deterioration of the environment. Overall, pre-weaning enrichment increased aggression after weaning whereas post-weaning enrichment reduced it, and personality type related to some aspects of fighting behaviour. (C) 2011 Elsevier B.V. All rights reserved.

Are there risk factors in alpine skiing? A controlled multicentre survey of 1278 skiers

OBJECTIVE: To analyse risk factors in alpine skiing. DESIGN: A controlled multicentre survey of injured and non-injured alpine skiers. SETTING: One tertiary and two secondary trauma centres in Bern, Switzerland. PATIENTS AND METHODS: All injured skiers admitted from November 2007 to April 2008 were analysed using a completed questionnaire incorporating 15 parameters. The same questionnaire was distributed to non-injured controls. Multiple logistic regression was performed. Patterns of combined risk factors were calculated by inference trees. A total of 782 patients and 496 controls were interviewed. RESULTS: Parameters that were significant for the patients were: high readiness for risk (p = 0.0365, OR 1.84, 95% CI 1.04 to 3.27); low readiness for speed (p = 0.0008, OR 0.29, 95% CI 0.14 to 0.60); no aggressive behaviour on slopes (p<0.0001, OR 0.19, 95% CI 0.09 to 0.37); new skiing equipment (p = 0.0228, OR 59, 95% CI 0.37 to 0.93); warm-up performed (p = 0.0015, OR 1.79, 95% CI 1.25 to 2.57); old snow compared with fresh snow (p = 0.0155, OR 0.31, 95% CI 0.12 to 0.80); old snow compared with artificial snow (p = 0.0037, OR 0.21, 95% CI 0.07 to 0.60); powder snow compared with slushy snow (p = 0.0035, OR 0.25, 95% CI 0.10 to 0.63); drug consumption (p = 0.0044, OR 5.92, 95% CI 1.74 to 20.11); and alcohol abstinence (p<0.0001, OR 0.14, 95% CI 0.05 to 0.34). Three groups at risk were detected: (1) warm-up 3-12 min, visual analogue scale (VAS)(speed) >4 and bad weather/visibility; (2) VAS(speed) 4-7, icy slopes and not wearing a helmet; (3) warm-up >12 min and new skiing equipment. CONCLUSIONS: Low speed, high readiness for risk, new skiing equipment, old and powder snow, and drug consumption are significant risk factors when skiing. Future work should aim to identify more precisely specific groups at risk and develop recommendations--for example, a snow weather index at valley stations.

Crecimiento y recubrimiento de Azorella monantha Clos (Apiaceae) en los altos Andes Centrales de Mendoza, Argentina

Azorella monantha Clos (Apiaceae) es una planta en cojín que se desarrolla desde 2900 a 4000 m s.n.m. en los Andes Centrales de Mendoza, Argentina. Su población comprende individuos de diferentes formas y tamaño. Estos cojines muestran alta ocurrencia en sitios llanos y base de laderas donde hasta se superponen formando carpetas o alfombras más o menos continuas. Ellos presentan el fenómeno de recubrimiento al cubrir todos los obstáculos que aparecen en la expansión de sus crecimientos como bloques de piedras, ripio, plantas y hasta sus propios individuos. Estos hechos de recubrimientos señalan la alta capacidad de estos cojines de ocupar los espacios disponibles. En el área estudiada su crecimiento en diámetro lateral fue de 1,15 a 1, 90 cm/año (promedio de 1, 52 cm/año). Observaciones y mediciones de los cojines revelan el carácter agresivo de los mismos sobre los obstáculos y plantas invasoras que temporalmente ocupan los sitios de los espacios vacíos abiertos en sus superficies. Con esto último se demuestra su carácter totalmente opuesto al de una verdadera nodriza como se le atribuye a él y a otros cojines andinos.

The influence of sulfuric environments on concretes elaborated with sulfate resistant cements and mineral admixtures. Part 1: Concrete exposed to Sodium Sulfate (Na2SO4) = Estudio de la influencia de los medios con presencia de sulfatos en hormigones con cementos sulforresistentes y adiciones minerales. Parte 1: Hormigones expuestos a sulfato sódico (Na2SO4)

The study of sulfate attack in concrete is considered vital for the preservation of the structural integrity of constructions. Its aggressive behaviour causes degradation of the cement matrix which changes the initial properties of the material. In this article, the sulfate resistance of concrete is studied. To that goal, four different concrete mixes were made with sulphur resistant cement. The concretes were tested for compressive strength, transport capacity of sulfates and microstructural properties. An experimental program was proposed in which the concrete samples were submerged in sodium sulphate (Na2SO4) solution. The obtained results were compared with reference values of concretes cured in calcium hydroxide [Ca(OH)2]. According to the results the concrete with ground granulated blast-furnace slag presented the best behavior when exposed to sodium sulphate (Na2SO4) solution. El estudio del ataque de sulfatos en el hormigón se considera de gran importancia para la conservación de la integridad estructural de las construcciones. Su agresividad se basa en la degradación de la matriz cementicia modificando las características iniciales de diseño. En el presente trabajo se estudia la resistencia del hormigón al ataque de sulfatos provenientes de sulfato sódico (Na2SO4). Para llevar a cabo la investigación se diseñaron cuatro dosificaciones de hormigón empleando cementos sulforresistentes y adiciones minerales. Se llevó a cabo una propuesta experimental donde las muestras de hormigón se sumergieron en disolución de sulfato sódico (Na2SO4) de concentración 1M. Posteriormente se realizaron ensayos de resistencia mecánica, capacidad de transporte de sulfatos y propiedades microestructurales, a distintas edades. Los resultados obtenidos se compararon con valores de referencia de mezclas de hormigón curadas expuestas a hi-dróxido cálcico [Ca(OH)2]. De acuerdo a los resultados obtenidos, el hormigón con escoria de alto horno presentó las mejores características de durabilidad frente a sulfatos provenientes de sulfato sódico

A eficácia e segurança dos psicofármacos em crianças e adolescentes

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Europe’s energy security – is the Energy Union the answer? EPC Commentary

From the Introduction. The EU has seen a renewed interest in strengthening its common energy policy and promoting energy security. The greatest trigger has been Russia’s unpredictable, aggressive behaviour in Ukraine in the past year. Lack of trust in a long-standing economic partner and concerns about relying on a trade partner that does not adhere to international norms have left their mark.

Masking odour when regrouping rabbit does: Effect on aggression, stress and lesions.

Regrouping female rabbits (Oryctolagus cuniculus) in group housing systems is a common management practice in Swiss rabbit breeding which may, however, induce agonistic interactions resulting in social stress and severe lesions. On farms using artificial insemination, does are usually kept singly for 12 days after parturition to avoid pseudopregnancy and fighting for nests. The integration of new group members usually occurs after this isolation phase. This study was conducted with 128 gravid does of the Hycole hybrid, housed in pens covering a floor area of 5.7 m2 that was bedded with straw and furnished with elevated areas, hiding places and eight compartments with nest boxes. In the experiment, the fur of 16 groups of 8 does each was sprayed with either alcohol or vinegar to mask the pre-existing group odours, or with water (control groups) shortly before regrouping. Lesion scores, stress parameters (body temperature and blood glucose level) and behaviour were assessed before and after the isolation phase. Effects of treatment and time on all collected parameters were analysed using mixed models. On the second day after regrouping 43% of the does showed new lesions. In the first five days after regrouping, new lesions occurred in 60% of the does; 32% had severe lesions. After regrouping, more agonistic interactions were observed and body temperature and blood glucose levels were higher than before regrouping (P<0.001 each). Body temperature increased less in groups treated with vinegar compared to the other two treatments on the first day after regrouping (P=0.017). In all other parameters no influence of the treatment with alcohol or vinegar was found. These findings suggest that masking the group odours with alcohol or vinegar had little effect on lesions, stress and agonistic interactions. Therefore, alternative management procedures need to be developed to reduce lesions and stress caused by aggressive behaviour.

Experience overrides colour in lizard contests

We examined the role of conspicuous coloration in male-male contests for two species of Australian dragon lizards, Ctenophortis decresii and C. vadnappa, in which conspicuous coloration has a demonstrated predation cost. We conducted contests in which the overall conspicuousness of male coloration was manipulated using paints that matched the spectral reflectance of the lizards, as well as natural (control) contests. There was little evidence for an influence of colour on contest outcome or aggression levels for either species when all experiments were considered. However, we found a significant effect of trial order and experience on contest outcome and aggression levels (the same pair of males was used for both types of contest), despite a 2-3 week interval between contests. When we examined only the first trial between unfamiliar males, we found that male C. vadnappa that had been painted to appear more conspicuous consistently won. Comparison with the natural trials suggests that the aspect of colour manipulation that was responsible for this result was the 'hue' of the throat: males with yellower throats consistently beat males with bluer throats in both natural and painted trials. The difference in coloration of flank markings also predicted the difference in aggression scores between contestants in the natural trials. These results suggest that although colour is important in opponent assessment and in determining contest outcome in C vadnappa, previous agonistic experience can override the effects of colour and have a long-lasting influence on aggressive behaviour.

The clinical effectiveness of a multisensory therapy on clients with developmental disability

Many clients in Hong Kong with developmental disabilities stay in mental hospitals because of mental disorders and behavioural problems. There is a need to identify strategies that promote psychological well-being and reduce problem behaviours in this group of clients. This study evaluates the impact of multisensory therapy on participants’ emotional state, level of relaxation, challenging behaviour, stereotypic self-stimulating behaviour (SSB) and adaptive behaviour (AB). Using an experimental design, 89 participants were recruited from a developmental disability unit in a hospital in Hong Kong and randomly assigned to either an experimental (n = 48) or a control group (n = 41). Multisensory therapy sessions (n = 36) were conducted with experimental group and activity sessions (n = 36) were conducted with controls for 12 weeks. Multisensory therapy promoted participants’ positive emotions and relaxation. However, there was no evidence that multisensory therapy was superior to activity therapy in reducing aggressive behaviour and stereotypic self-stimulating behaviour or promoting adaptive behaviour. The key variables that influence clients’ behaviours in the multisensory therapy may be related to the relationship with the carer, constant environment, relaxation and freedom from demands rather than sensory input. Multisensory therapy could be used to provide leisure and promote psychological well-being, rather than for reducing problem behaviour.

The development of a short scale measuring aggressiveness and anger in competitive athletes

Objectives: The study of aggression and anger in competitive sport relies on accurate and economical measurement via observation, interview and questionnaire. Unfortunately, extant questionnaires have been criticised for having poor validity, are not sport specific, or reflect mood states rather than trait qualities. Therefore, a measure of trait anger and aggressiveness in competitive athletes was developed. Method: A list of statements representing aggressiveness and anger was generated and distributed to competitive athletes from diverse sports. Exploratory and confirmatory analyses were used to verify the theoretically predicted factor structure. Correlations with an extant measure of aggression and anger were used to ascertain concurrent validity. Discriminant validity was tested by comparing males with females, and aggressive with non-aggressive footballers. Results: A 12-item scale (Competitive Aggressiveness and Anger Scale, CAAS) consisting of two subscales was derived using principal component factor analysis with oblimin rotation. Confirmatory factor analysis using structural equation modelling confirmed the overall structure. Test-retest correlation, construct and discriminant validities were good, supporting the utility of the scale as a measure of athlete trait aggressiveness and anger. Conclusions: The CAAS appears to be a useful measure of athletic anger and aggressiveness. Its brevity and ability to discriminate aggressive from non-aggressive athletes should prove useful for future research concerning aggressive behaviour in competitive athletes. © 2006 Elsevier Ltd. All rights reserved.

Anger rumination and self-reported aggression amongst British and Hong Kong Chinese athletes: a cross cultural comparison

Previous work has determined relationships between provocation, anger rumination, and aggression in British athletes (Maxwell, 2004); however, the reliability of these findings and their generality across diverse cultures has not been examined. Therefore, a comparison of British and Hong Kong (HK) Chinese athletes’ propensity for rumination and aggression was undertaken. Provocation and thoughts of revenge were significantly associated with the self-reported aggression of both British and HK Chinese athletes. Frequency of aggression was similar across cultures except for a tendency for British male contact sport athletes to report greater frequency of aggressive behaviour. HK Chinese athletes tended to report higher frequencies of thoughts relating to understanding the causes of anger and higher incidence of perceived provocation. It was concluded that the pattern of aggressive behaviour was similar across the two cultures when opportunities for aggression are infrequent, but that HK Chinese athletes may inhibit aggressive responding even when opportunities are frequent.

Cyberbullying: estudo da prevalência de comportamentos de cyberbullying e sua relação com vivências de vergonha e estados emocionais negativos

Objectivos: O presente estudo tem como principal objectivo avaliar a prevalência dos comportamentos de cyberbullying, analisando a influência de variáveis sóciodemográficas, e, compreender a sua relação com as vivências de vergonha interna e externa e com os estados emocionais negativos, particularmente a depressão, a ansiedade e o stress. Método: Para a recolha de dados recorreu-se a uma mostra de adolescentes (N=131) a frequentar o 3º ciclo do ensino básico, com idades compreendidas entre os 12 e os 18 anos (M= 13,76; DP= 1,25). O protocolo de avaliação foi constituído por um questionário psicossocial desenvolvido especificamente para este estudo e por um conjunto de medidas fidedignas para avaliar o cyberbullying (CBQ e CBQ-V), a vergonha interna (ISS), vergonha externa (OAS) e os estados emocionais negativos (DASS-21). Resultados: Os nossos dados revelaram que 76 adolescentes (58%) exerceram um qualquer comportamento de cyberbullying (com um predomínio dos rapazes), enquanto 50 adolescentes (38,2%) já foram vítimas de um qualquer comportamento de cyberbullying (com igual proporção entre rapazes e raparigas). Manter lutas e discussões online, usando insultos mediante mensagens electrónicas foi o comportamento praticado mais frequente (30,5%), enquanto o ser removido intencionalmente de um grupo online foi o comportamento sofrido mais frequente (16,1%). A idade e os anos de reprovações mostraram uma associação positiva com os comportamentos de agressão por cyberbullying. Foi ainda analisada a sobreposição entre a execução e a vitimização de comportamentos de cyberbullying, tendo sido discriminados quatro grupos de adolescentes: só agressores (adolescentes que apenas exerceram comportamentos de agressão), só vítimas (apenas sofreram comportamentos de cyberbulling), vítimas e agressores (adolescentes que são simultaneamente agressores e vítimas), e nem vítimas nem agressores (adolescentes que não exerceram nem sofreram qualquer comportamento de cyberbullying). Os resultados evidenciaram que quanto maior a frequência de comportamentos de agressão por cyberbullying, maior a vergonha interna e maior os níveis de stress demonstrados. Por sua vez, quanto maior a frequência de vitimização por cyberbullying, maior a vergonha interna e externa, bem como maior os níveis de ansiedade e stress. Conclusão: Devido a complexidade do fenómeno cyberbullying e seu recente surgimento, serão necessários mais estudos, particularmente longitudinais, para compreender a relação antecedente e/ou consequentes aos comportamentos de cyberbullying entre estados emocionais negativos e as experiências de vergonha. / Objectives: The present study has as main objective to assess the prevalence of cyberbullying behaviours, analyzing the influence of socio demographic variables, and, understand its relationship to the experiences of internal and external shame and negative emotional states, particularly depression, anxiety and stress. Method: For data collection we used a sample of adolescents (N=131) attending the 3rd cycle of basic education, aged between 12 and 18 years (M=13,76; SD=1,25). The evaluation protocol consisted of a psychosocial questionnaire developed specifically for this study and a set of reliable measure to assess cyberbullying (CBQ and CBQ-V), internal shame (ISS), external shame (OAS) and the emotional states negative (DASS-21). Results: Our data indicate that 76 adolescents (58%) exerted any conduct of a cyberbullying (with a predominance of boys), while 50 adolescents (38,2%) had been victims of cyberbullying behaviour of any one (with an equal ratio of boys and girls). Keep fighting and discussions online, through e-mails using insults behaviour was practiced more often (30,5%), while being intentionally removed a group of online behaviour is seen more frequently (16,1%). The age and years of failures were positively associated with the behaviours of aggression by cyberbullying. Was further examined the overlap between enforcement and victimization of cyberbullying behaviours, having been discriminated four groups of adolescents: only aggressors (adolescents who have had only aggressive behaviour), only victims (only suffered cyberbullying behaviours), victims and aggressors (adolescents who are both perpetrators and victims), and neither victims nor aggressors (adolescents who did not exercise any behaviour or suffered cyberbullying). Results showed that the higher the frequency of aggression by cyberbullying behaviour, the greater shame and internal stress levels demonstrated. In turn, the higher the frequency of cyberbullying victimization, the greater the shame internal and external, as well as higher levels of anxiety and stress. Conclusion: Due to the complexity of the phenomenon cyberbullying and its recent emergence, further studies are needed, particularly longitudinal, to understand the relationship between antecedent and/or consequential to cyberbullying behaviours between negative emotional states and experiences of shame.

Retour post-isolement en milieu psychiatrique : développement, implantation et évaluation d’une intervention en soins aigus

L’isolement avec ou sans contention (IC) en milieu psychiatrique touche près d’un patient sur quatre au Québec (Dumais, Larue, Drapeau, Ménard, & Giguère-Allard, 2011). Il est pourtant largement documenté que cette pratique porte préjudice aux patients, aux infirmières et à l’organisation (Stewart, Van der Merwe, Bowers, Simpson, & Jones, 2010). Cette mesure posant un problème éthique fait l’objet de politiques visant à la restreindre, voire à l’éliminer. Les études sur l’expérience de l’isolement du patient de même que sur la perception des infirmières identifient le besoin d'un retour sur cet évènement. Plusieurs équipes de chercheurs proposent un retour post-isolement (REPI) intégrant à la fois l’équipe traitante, plus particulièrement les infirmières, et le patient comme intervention afin de diminuer l’incidence de l’IC. Le REPI vise l’échange émotionnel, l’analyse des étapes ayant mené à la prise de décision d’IC et la projection des interventions futures. Le but de cette étude était de développer, implanter et évaluer le REPI auprès des intervenants et des patients d’une unité de soins psychiatriques aigus afin d’améliorer leur expérience de soins. Les questions de recherche étaient : 1) Quel est le contexte d’implantation du REPI? 2) Quels sont les éléments facilitants et les obstacles à l’implantation du REPI selon les patients et les intervenants? 3) Quelle est la perception des patients et des intervenants des modalités et retombées du REPI?; et 4) L’implantation du REPI est-elle associée à une diminution de la prévalence et de la durée des épisodes d’IC? Cette étude de cas instrumentale (Stake, 1995, 2008) était ancrée dans une approche participative. Le cas était celui de l’unité de soins psychiatriques aigus pour premier épisode psychotique où a été implanté le REPI. En premier lieu, le développement du REPI a d’abord fait l’objet d’une documentation du contexte par une immersion dans le milieu (n=56 heures) et des entretiens individuels avec un échantillonnage de convenance (n=3 patients, n=14 intervenants). Un comité d’experts (l’étudiante-chercheuse, six infirmières du milieu et un patient partenaire) a par la suite développé le REPI qui comporte deux volets : avec le patient et en équipe. L’évaluation des retombées a été effectuée par des entretiens individuels (n= 3 patients, n= 12 intervenants) et l’examen de la prévalence et de la durée des IC six mois avant et après l’implantation du REPI. Les données qualitatives ont été examinées selon une analyse thématique (Miles, Huberman, & Saldana, 2014), tandis que les données quantitatives ont fait l’objet de tests descriptifs et non-paramétriques. Les résultats proposent que le contexte d’implantation est défini par des normes implicites et explicites où l’utilisation de l’IC peut générer un cercle vicieux de comportements agressifs nourris par un profond sentiment d’injustice de la part des patients. Ceux-ci ont l’impression qu’ils doivent se conformer aux attentes du personnel et aux règles de l’unité. Les participants ont exprimé le besoin de créer des opportunités pour une communication authentique qui pourrait avoir lieu lors du REPI, bien que sa pratique soit variable d’un intervenant à un autre. Les résultats suggèrent que le principal élément ayant facilité l’implantation du REPI est l’approche participative de l’étude, alors que les obstacles rencontrés relèvent surtout de la complexité de la mise en œuvre du REPI en équipe. Lors du REPI avec le patient, les infirmières ont pu explorer ses sentiments et son point de vue, ce qui a favorisé la reconstruction de la relation thérapeutique. Quant au REPI avec l’équipe de soins, il a été perçu comme une opportunité d’apprentissage, ce qui a permis d’ajuster le plan d’intervention des patients. Suite à l’implantation du REPI, les résultats ont d’ailleurs montré une réduction significative de l’utilisation de l’isolement et du temps passé en isolement. Les résultats de cette thèse soulignent la possibilité d’outrepasser le malaise initial perçu tant par le patient que par l’infirmière en systématisant le REPI. De plus, cette étude met l’accent sur le besoin d’une présence authentique pour atteindre un partage significatif dans la relation thérapeutique, ce qui est la pierre d’assise de la pratique infirmière en santé mentale. Cette étude contribue aux connaissances sur la prévention des comportements agressifs en milieu psychiatrique en documentant le contexte dans lequel se situe l’IC, en proposant un REPI comportant deux volets de REPI et en explorant ses retombées. Nos résultats soutiennent le potentiel du développement d’une prévention tertiaire qui intègre à la fois la perspective des patients et des intervenants.