980 resultados para Elisa S. Moncarz
Resumo:
Tesis (Maestría en Artes con Especialidad en Educación) U.A.N.L.
Resumo:
Tesis (Maestría en Ciencias con Especialidad en Entomología Médica) U.A.N.L.
Resumo:
Tesis (Maestría en Ciencias con acentuación en Microbiología) UANL, 2014.
Resumo:
UANL
Resumo:
Resumen basado en el de la autora
Resumo:
En el universo urbano, confluye un sinnúmero de sentires, tradiciones y saberes de lo que significa habitar la ciudad latinoamericana. La literatura escrita por mujeres, en diálogo abierto con dichas expresiones nos da la posibilidad de explorar esos contenidos y reinterpretar la realidad. Es posible que sea necesario emular formas combativas y propuestas transformadoras de la realidad. En Colombia, la obra narrativa de Elisa Mújica nos permite indagar en el contexto espacio-temporal narrado, as como en las relaciones de poder establecidas en ciudades urbanas como Bogotá: la ciudad es el escenario donde mujer y ciudad son dos sujetos protagónicos que dialogan estrechamente y están asidos por la misma cuerda: el devenir. En la presente investigación, el lector hallará un recorrido por la recepción de la obra de Elisa Mújica, as como también por los diferentes lugares, espacios e imaginarios urbanos presentes en la novela, los que a su vez en diálogo con el mundo interior de su protagonista, posibilitarán el hallazgo de la concienciación y de los efectos que se deriven de ese encuentro. La investigación efectúa un análisis cultural, crítico y semiótico, que indaga en la relación establecida entre mujer y ciudad (Bogotá, años 1930-1980) y desde allí, plantea la urgente necesidad de asumir la concienciación en ese tiempo y espacio habitados. En este sentido, la concienciación constituye un fenómeno social cuestionador del imaginario social patriarcal y desde la literatura, (concretamente, desde la narrativa) en un intento por contribuir a la transformación de ese imaginario, se proponen nuevos tipos de relaciones de género.
Resumo:
La presente investigación trata de los cambios de la Gestión Curricular en el Centro Infantil del Buen Vivir (CIBV) “Elisa Mariño de Carvajal” de la ciudad de Guaranda en los períodos 2011-2013. Para ello se ha elaborado cuatro capítulos con las siguientes temáticas: En el primer capítulo, se explica las características y fundamentos rectores del CIBV; en el segundo, se detallan los procesos de desarrollo de la primera infancia; el tercero contiene los instrumentos para la investigación; el cuarto capítulo se presenta los resultados obtenidos; y por último, está investigación pretende proponer un Plan de Mejora a la Gestión Curricular para que la Coordinadora, Educadoras y padres/madres de familia brinden una atención de calidad a las niñas/os de 1 a 3 años de edad. Este trabajo comprende un análisis profundo de la primera infancia, la cual debe ser protegida, estimulada y atendida de la mejor manera. Las niñas y niños de 0 a 3 años experimentan un proceso de cambios; biológicos, psicológicos y sociales, por ello es de vital importancia que los programas de atención a la primera infancia como los Centros Infantiles del Buen Vivir (CIBV), brinden las garantías necesarias para su óptimo desarrollo.
Resumo:
Raised levels of chylomicrons and chylomicron remnants, which circulate following a meal, have been implicated in the development of atherosclerosis. Apolipoprotein (apo) B-48 is exclusively associated with chylomicron particles and provides a specific direct measurement of the number of intestinally derived lipoproteins in the circulation. The quantification of apo B-48 in biological samples is difficult due to the very low concentration in plasma, structural similarity to the N-terminal 48% of apo B-100 and lack of an appropriate standard for apo B-48. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), followed by coomassie blue staining, has been used for many years to measure apo B-48 levels in triacylglycerol (TAG)-rich lipoprotein samples. The raising of antiserum to apo B-48 has led to development of more sensitive and specific methods including immunoblotting and enzyme-linked immunosorbant assays (ELISAs). This has enabled direct measurement of apo B-48 in plasma without the need for separation into TAG-rich lipoproteins. A high degree of variability was observed in the apo B-48 concentrations reported in the literature both within and between the SDS-PAGE, immunoblotting and ELISA methods. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
Resumo:
We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200µm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.
Resumo:
Background: Oxidative modification of low-density lipoprotein (LDL) plays a key role in the pathogenesis of atherosclerosis. LDL(-) is present in blood plasma of healthy subjects and at higher concentrations in diseases with high cardiovascular risk, such as familial hypercholesterolemia or diabetes. Methods: We developed and validated a sandwich ELISA for LDL(-) in human plasma using two monoclonal antibodies against LDL(-) that do not bind to native LDL, extensively copper-oxidized LDL or malondialdehyde-modified LDL. The characteristics of assay performance, such as limits of detection and quantification, accuracy, inter- and intra-assay precision were evaluated. The linearity, interferences and stability tests were also performed. Results: The calibration range of the assay is 0.625-20.0 mU/L at 1: 2000 sample dilution. ELISA validation showed intra- and inter- assay precision and recovery within the required limits for immunoassays. The limits of detection and quantification were 0.423 mU/L and 0.517 mU/L LDL(-), respectively. The intra- and inter- assay coefficient of variation ranged from 9.5% to 11.5% and from 11.3% to 18.9%, respectively. Recovery of LDL(-) ranged from 92.8% to 105.1%. Conclusions: This ELISA represents a very practical tool for measuring LDL(-) in human blood for widespread research and clinical sample use. Clin Chem Lab Med 2008; 46: 1769-75.
Resumo:
In the present study, the validation of an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of canine brucellosis is described. Two different antigenic extracts, obtained by heat or ultrasonic homogenization of microbial antigens from a wild isolate of Brucella canis bacteria, were compared by ELISA and Western blot (WB). A total of 145 canine sera were used to define sensitivity, specificity and accuracy of the ELISA as follows: (1) sera from 34 animals with natural B. canis infection, confirmed by blood culture and PCR, as well as 51 sera samples from healthy dogs with negative results by the agar-gel immunodiffusion (ACID) test for canine brucellosis, were used as the control panel for B. cants infection; and (2) to scrutinize the possibility of cross reactions with other common dog infections in the same geographical area in Brazil, 60 sera samples from dogs harboring known infections by Leptospira sp., Ehrlichia canis, canine distemper virus (CDV), Neospora caninum, Babesia canis and Leishmania chagasi (10 in each group) were included in the study. The ELISA using heat soluble bacterial extract (HE-antigen) as antigen showed the best values of sensitivity (91.18%), specificity (100%) and accuracy (96.47%). In the WB analyses, the HE-antigen showed no cross-reactivity with sera from dogs with different infections, while the B. canis sonicate had various protein bands identified by those sera. The performance of the ELISA standardized with the heat soluble B. canis antigen indicates that this assay can be used as a reliable and practical method to confirm infection by this microorganism, as well as a tool for seroepidemiological studies. (C) 2010 Elsevier Ltd. All rights reserved.
Resumo:
Bill & Melinda Gates Foundation[51308]