Physical linkage to drug resistance genes results in conservation of var genes among west pacific Plasmodium falciparum isolates

The multicopy var gene family encoding the variant surface antigen Plasmodium falciparum erythrocyte membrane protein 1 is highly diverse, with little overlap between different P. falciparum isolates. We report 5 var genes (varS1-varS5) that are shared at relatively high frequency among 63 genetically diverse P. falciparum isolates collected from 5 islands in the West Pacific region. The varS1, varS2, and varS3 genes were localized to the internal region on chromosome 4, similar to 200 kb from pfdhfr-ts, whereas varS4 and varS5 were mapped to an internal region of chromosome 7, within 100 kb of pfcrt. The presence of varS2 and varS3 were significantly correlated with the pyrimethamine-resistant pfdhfr genotype, whereas varS4 was strongly correlated with the chloroquine-resistant pfcrt genotype. Thus, the conservation of these var genes is the result of their physical linkage with drug-resistant genes in combination with the antimalarial drug pressure in the region.

Supervisão e colaboração no ensino das ciências : estudo de caso realizado numa escola do ensino básico em Díli

O sistema educativo de Timor-Leste tem assistido, desde o momento da independência do país, a uma reorganização e a um desenvolvimento no sentido de aperfeiçoar os níveis de formação e de qualificação dos cidadãos timorenses. A intencionalidade é a de permitir o progresso social, económico, tecnológico e cultural de que o país precisa. Neste quadro, a formação inicial e contínua de professores assume um papel fulcral para o cumprimento das metas estabelecidas, sendo este o contexto principal onde a minha atividade profissional se desenvolveu. Este estudo situou-se na supervisão e colaboração de práticas letivas com uma professora de ensino básico, do sexto ano, no ensino das ciências, tendo por base os pressupostos teóricos da educação em ciências. O estudo que foi desenvolvido numa Escola Básica Central, em Díli, Timor- Leste, tem por base os pressupostos inerentes à supervisão, designadamente: os encontros de pré observação, observação e pós-observação, sustentados pela colaboração da investigadora no processo de desenvolvimento profissional da professora participante Os principais objetivos do estudo centraram-se em identificar e compreender quais as conceções da professora participante no estudo relativamente ao ensino das ciências e, simultaneamente, a implementação de práticas de ensino relacionadas com a didática das ciências, assumindo como referencial que as conceções em ciências que os professores possuem têm implicações no modo como ensinam (Praia, Cachapuz & Pérez, 2002). Mais especificamente, pretendeu-se analisar e compreender a forma como a professora responsável pelo ensino da disciplina de estudo do meio no numa turma do 6º ano desenvolve as suas atividades letivas . A disciplina de estudo do meio contém conteúdos da área das ciências da natureza e das ciências sociais, no entanto, este estudo desenvolveu-se apenas no âmbito das ciências naturais, por se tratar da minha área específica de formação e aquela que escolhi para aprofundar os meus conhecimentos. A investigação desenvolvida assumiu o paradigma qualitativo/interpretativo e enquadrou-se num design de estudo de caso. Para Coutinho & Chaves (2002), um aluno, um professor, uma turma, uma escola, a prática de um professor ou uma política educativa são apenas alguns exemplos de variáveis educativas para as quais o estudo de caso é a metodologia que melhor se aplica. A recolha de informação para a investigação realizou-se no decorrer dos encontros de preparação das atividades letivas, da observação de aulas e do registo realizado, dos encontros pós-observação, das entrevistas e da análise documental de interesse sobre o tema.

Pronounced genetic population structure in a potentially vagile fish species (Pristipomoides multidens, Teleostei; Perciformes; Lutjanidae) from the East Indies triangle

The East Indies triangle, bordered by the Phillipines, Malay Peninsula and New Guinea, has a high level of tropical marine species biodiversity. Pristipomoides multidens is a large, long-lived, fecund snapper species that is distributed throughout the East Indies and Indo-Pacific. Samples were analysed from central and eastern Indonesia and northern Australia to test for genetic discontinuities in population structure. Fish (n = 377) were collected from the Indonesian islands of Bali, Sumbawa, Flores, West Timor, Tanimbar and Tual along with 131 fish from two northern Australian locations (Arafura and Timor Seas) from a previous study. Genetic variation in the control region of the mitochondrial genome was assayed using restriction fragment length polymorphism and direct sequencing. Haplotype diversity was high (0.67-0.82), as was intraspecific sequence divergence (range 0-5.8%). FST between pairs of populations ranged from 0 to 0.2753. Genetic subdivision was apparent on a small spatial scale; FST was 0.16 over 191 km (Bali/Sumbawa) and 0.17 over 491 km (Bali/Flores). Constraints to dispersal that contribute to, and maintain, the observed degree of genetic subdivision are experienced presumably by all life history stages of this tropical marine finfish. The constraints may include (1) little or no movement of eggs or larvae, (2) little or no home range or migratory movement of adults and (3) loss of larval cohorts due to transport of larvae away from suitable habitat by prevailing currents

Plus ça change: (Post)Colonial Timor-Leste as a Return to (Pre)Colonial Realities in the Novels of Luis Cardoso

This article examines the novels of the East Timorese writer Luís Cardoso, and argues that their representations of a colonial past should not be simply interpreted as memorializations of Timor-Leste’s suffering at the hands of foreign aggressors. It proposes that underlying their revisiting of the past is a call for acknowledgement of the agency of East Timorese in the history of violent conflict that has troubled the nation, and that only this can guarantee true reconciliation, justice and national independence.

Magnesium/Calcium ratios and sea surface temperature estimation for sediments of the Timor Sea

We use a multiproxy approach to monitor changes in the vertical profile of the Indonesian Throughflow as well as monsoonal wind and precipitation patterns in the Timor Sea on glacial-interglacial, precessional, and suborbital timescales. We focus on an interval of extreme climate change and sea level variation: marine isotope (MIS) 6 to MIS 5e. Paleoproductivity fluctuations in the Timor Sea follow a precessional beat related to the intensity of the Australian (NW) monsoon. Paired Mg/Ca and d18O measurements of surface- and thermocline-dwelling planktonic foraminifers (G. ruber and P. obliquiloculata) indicate an increase of >4°C in both surface and thermocline water temperatures during Termination II. Tropical sea surface temperature changed synchronously with ice volume (benthic d18O) during deglaciation, implying a direct coupling of high- and low-latitude climate via atmospheric and/or upper ocean circulation. Substantial cooling and freshening of thermocline waters occurred toward the end of Termination II and during MIS 5e, indicating a change in the vertical profile of the Indonesian Throughflow from surface- to thermocline-dominated flow.

Age determination and age tie-points of sediment cores from the Timor Sea

The evolution of the Australian monsoon in relation to high-latitude temperature fluctuations over the last termination remains highly enigmatic. Here we integrate high-resolution riverine runoff and dust proxy data from X-ray fluorescence scanner measurements in four well-dated sediment cores, forming a NE-SW transect across the Timor Sea. Our records reveal that the development of the Australian monsoon closely followed the deglacial warming history of Antarctica. A minimum in riverine runoff documents dry conditions throughout the region during the Antarctic Cold Reversal (15-12.9 ka). Massive intensification of the monsoon coincided with Southern Hemisphere warming and intensified greenhouse forcing over Australia during the atmospheric CO2 rise at 12.9-10 ka. We relate the earlier onset of the monsoon in the Timor Strait (13.4 ka) to regional changes in landmass exposure during deglacial sea-level rise. A return to dryer conditions occurred between 8.1 and 7.3 ka following the early Holocene runoff maximum.

Analysis of ferromanganiferous sedimentary rocks from Timor

Seven manganese nodules, eight ferromanganiferous shales from the Cretaceous Wai Bua Formation of Timor, and a pelagic limestone with four ferromanganese enriched layers from the Middle Eocene of Timor have been analysed. The nodules are compared with modern deep-sea nodules, and the ferromanganiferous shales are contrasted with relatively shallow marine manganiferous shales. The conclusion is reached that these rocks from Timor were probably deposited in a bathypelagic environment. There is a total absence of any indication that volcanic material has contributed to these deposits. The chemical composition of the ferromanganiferous rocks are discussed and some indications of biogenic influences are noted. The Middle Eocene pelagic limestone is compared with a similar modern sediment described from the Easter Island Rise in the Pacific.