Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification

A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. (c) 2008 Elsevier B.V. All rights reserved.

Neutron and proton diffusion in fusion reactions for the synthesis of superheavy nuclei

The restriction of the one dimensional (1D) master equation (ME) with the mass number of the projectile-like fragment as a variable is studied, and a two-dimensional (2D) master equation with the neutron and proton numbers as independent variables is set up, and solved numerically. Our study showed that the 2D ME can describe the fusion process well in all projectile-target combinations. Therefore the possible channels to synthesize super-heavy nuclei can be studied correctly in wider possibilities. The available condition for employing 1D ME is pointed out.

The beta decay of N-21

The beta-delayed neutron and gamma energy spectra taken from the decay of neutron-rich nucleus N-21 were measured by using the beta - gamma and beta - n coincidence detection method. Thirteen new neutron groups ranging from 0.28MeV to 4.98 MeV and with a total branching ratio of 88.7 +/- 4.2% were observed and presented. One gamma transition with an energy of 1222 keV emitted from the excited state of O-21, and four gamma transitions with energies of 1674, 2397, 2780, and 3175 keV emitted from the excited states of O-20 were identified in the 3 decay chain of N-21. The beta decay half-life for N-21 is determined to be 82.9 +/- 1.9 ms. The uncertainty of half-life is much smaller than the previous result.

丰中子奇异核17B的实验研究

在兰州重离子加速器国家实验室(HIRFL)放射性次级束流线(RIBLL)上，用束流透射法测量了丰中子奇异核17B与C靶反应的总截面.假定17B具有15B(核芯)＋2n结构，采用Gauss＋HO形式的密度分布和零力程Glauber模型进行计算的结果可以很好地拟合实验数据，并得出17B的密度分布有一个很大的弥散，表明17B是双中子晕核.

Configuration of the valence neutrons of B-17

The reaction cross section of B-17 on C-12 target at (43.7 +/- 2.4) MeV/u has been measured at the Radioactive Ion Beam Line in Lanzhou (RIBLL). The root-mean-square matter radius (R-rms) was deduced to be (2.92 +/- 0.10) fm, while the R-rms of the core and the valence neutron distribution are 2.28 fm and 5.98 fm respectively. Assuming a "core plus 2n" structure in B-17, the mixed configuration of (2s(1/2)) and (1d(5/2)) of the valence neutrons is studied and the s-wave spectroscopic factor is found to be (80 +/- 21)%.

Slow highly charged ion O4+ induced electron emission from clean solid surfaces

The. total electron emission yields following the interaction of slow highly charged ions (SHCI) O4+ with different material surfaces (W, Au, Si and SiO2) have been measured. It is found that the electron emission yield gamma increases proportionally with the projectile velocity v ranging from 5.36 x 10(5)m/s to 10.7 x 10(5)m/s. The total emission yield is dependent on the target materials, and it turns out to follow the relationship gamma(Au) > gamma(Si)> gamma(W). The result shows that the electron emission yields are mainly determined by the electron stopping power of the target when the projectile potential energy is taken as a constant, which is in good agreement with the former studies

beta-delayed neutron decay of N-21

The beta-delayed neutron and gamma spectra of neutron-rich nucleus N-21 using beta-gamma and beta-n coincidence measurements were presented in this paper. Thirteen new neutron groups ranging from 0.28 MeV to 4.98 MeV and with a total branching ratio 88.7 +/- 4.2% were observed. One gamma transition among the excited states of O-21 and foury transitions among the excited states of O-20 were identified in the beta decay chain of N-21. The ungated half-life of 83.8 +/- 2.1 ms was also determined for N-21.

Efficiency calibration of neutron detector array for beta decay of exotic nuclei

The research of the in-beam efficiency calibration of Neutron Detector Array of Peking University using N-17 and C-16 beams was introduced in this paper. The efficiency of neutron wall and ball are comparable to the foreign similar devices and neutrons can be detected from low to high energies in high efficiency.

Isospin effect of projectile fragment yields

Isospin asymmetry is very important in the nuclear equation of state (EOS), isotope yield from the projectile fragments can give information of the reaction process. In this paper projectile fragment yields are measured in the collision Ar-36,Ar-40 + Ni-64 at incident energy 50 MeV/u with different isospin asymmetry project Ar-36,Ar-40, data analysis, particle identification and event selection are described. Isotope yields are compared in these two reactions, and axe also compared with the empirical parametrization of fragmentation cross-section calculated by EPAX.

The driving potential and cross sections for synthesizing super heavy nuclei with hot fusion

The dinuclear model of the formation mechanism of a superheavy compound nucleus assumes that when all nucleons of the projectile have been transferred in to the target nucleus the compound nucleus is formed. The nucleon transfer is determined by the driving potential. For some reaction channels, the relation between nucleon transfer and the evolution path of the neutron/proton ratio is rather complicated. In principle, both the dynamical equation and the driving potential should be a twodimensional explicit function of the neutron and proton. For the sake of simplicity we calculated the driving potential by choosing the path of the nucleon transfer which is related to the nutron/proton ratio, and the calculated evaporation residue cross-sections to synthesize the superheavy nuclei are much closer to the experimental data

Angular dispersion and deflection function for heavy ion elastic scattering

The differential cross sections for elastic scattering products of F-17 on Pb-208 have been measured. The angular dispersion plots of ln(d sigma/d theta) versus theta(2) are obtained from the angular distribution of the elastic scattering differential cross sections. Systematical analysis on the angular dispersion for the available experimental data indicates that there is an angular dispersion turning angle at forward angular range within the grazing angle. This turning angle can be clarified as nuclear rainbow in classical deflection function. The exotic behaviour of the nuclear rainbow angle offers a new probe to investigate the halo and skin phenomena.

Measurement of total reaction cross section for neutron-rich nucleus Li-11 on Si-28 target at medium energy

The neutron-rich nucleus Li-11 is separated by the radioactive ion beam line RIBLL at HIRFL from the breakup of 50MeV/u C-13 on Be target. The total reaction cross sections for Li-11 at energies range from 25 to 45MeV/u on Si target have been measured by using the transmission method. The experimental data at high and low energies can be fitted well by Glauber model using two Gauss density distribution. The matter radius of Li-11 was also deduced.

Possible way to synthesize superheavy element Z=117

Within the framework of the dinuclear system model, the production of superheavy element Z = 117 in possible projectile-target combinations is analysed systematically. The calculated results show that the production cross sections are strongly dependent on the reaction systems. Optimal combinations, corresponding excitation energies and evaporation channels are proposed, such as the isotopes Bk-248,Bk-249 in Ca-48 induced reactions in 3n evaporation channels and the reactions Sc-45+Cm-246,Cm-248 in 3n and 4n channels, and the system V-51+Pu-244 in 3n channel.

A transverse field gas ionization chamber performed at RIBLL

A transverse field gas ionization chamber as Delta E detector at the Radioactive Ion Beam Line in Lanzhou (RIBLL) is described. A high detection efficiency and long plateau are achieved with the mixed gas Ar(80%)+CO2(20%). The energy resolution is 3.25% for 4.94MeV alpha particle. This ionization chamber has been tested in the experiment with 50MeV/u Ni-58 bombarding Ta at RIBLL. All the fragments can be identified clearly by the ionization chamber.

Elastic scattering angular dispersion of weakly bound nucleus F-17

The differential cross-sections for elastic scattering of F-17 and O-17 on Pb-208 have been measured at Radioactive Ion Beam Line at Lanzhou (RIBLL). The variation of the logarithms of differential cross-sections with the square of scattering angles, viz. angulax dispersion plot, shows clearly that there exists a turning point in the range of small scattering angles (6 degrees-20 degrees) for F-17 due to its exotic structure, while no turning point was observed for O-17. The experimental results have been compared with previous data of other groups. Systematical analysis on the available data seems to conclude that there is an exotic behavior of elastic scattering angular dispersion of weakly bound nuclei with halo or skin structure as compared with that of the stable nuclei. Therefore the fact that the turning point of the elastic scattering angular dispersion plot appears at small angle for weakly bound nuclei can be used as a new probe to investigate the halo and skin phenomenon.