NMR structures of three single-residue variants of the human prion protein

The NMR structures of three single-amino acid variants of the C-terminal domain of the human prion protein, hPrP(121–230), are presented. In hPrP(M166V) and hPrP(R220K) the substitution is with the corresponding residue in murine PrP, and in hPrP(S170N) it is with the corresponding Syrian hamster residue. All three substitutions are in the surface region of the structure of the cellular form of PrP (PrPC) that is formed by the C-terminal part of helix 3, with residues 218–230, and a loop of residues 166–172. This molecular region shows high species variability and has been implicated in specific interactions with a so far not further characterized “protein X,” and it is related to the species barrier for transmission of prion diseases. As expected, the three variant hPrP(121–230) structures have the same global architecture as the previously determined wild-type bovine, human, murine, and Syrian hamster prion proteins, but with the present study two localized “conformational markers” could be related with single amino acid exchanges. These are the length and quality of definition of helix 3, and the NMR-observability of the residues in the loop 166–172. Poor definition of the C-terminal part of helix 3 is characteristic for murine PrP and has now been observed also for hPrP(R220K), and NMR observation of the complete loop 166–172 has so far been unique for Syrian hamster PrP and is now also documented for hPrP(S170N).

Nova Maris Caspii et regionis Usbeck, cum provincijs adjacentibus vera delineatio

in qua itinera Regia et alia notabiliora accurate denotantur per A. Maas ; reductio mappæ haijus a majore in hanc monirem formam facta est per Iohannem Petrum van Ghelen.

Institutiones in Linguam Hispanicam, admodum faciles, quales antehac numquam visae ... /

Mode of access: Internet.

Die landes-kultur-gesetzgebung des Preussischen staates; eine systematisch geordnete sammlung aller auf dieselbe bezug habenden gesetzlichen bestimmungen ... Nebst einem historisch-kritischen und praktischen kommentar über die betreffenden gesetze,

1. bd. Die allgemeine einleitung und die sammlung der verordnungen und reskripte.--2. bd. 1.-2. abth. Der kommentar.

Beantwortung auf die französischer Sprache erschienenen Schmähschrift, betitelt Anmerkungen über die Erklärung der Wiener Petersburger und Berliner Höfe, die Zergliederung der Republick Pohlen betreffend : gedruckt London, 1773 ... /

Signatures: )(⁸, A-I⁸, K⁴.

Ritusanhara, id est Tempestatum cyclus; carmen sanskritum, Kâlidâso adscriptum,

Sanskrit title at head of title-page.

Bhartriharis sententiae et carmen quod Chauri nomine circumfertur eroticum /

Mode of access: Internet.

Die Genesis, historisch-kritisch erläutert,

Mode of access: Internet.

Gamla Finland, eller Det fordna wiborgska gouvernementet.

No more published.

Österreich's Ehrenspiegel /

"A series of biographies by Franz Ziska, with portraits by J.D. Böhm." cf. Brit. Mus. Cat.

Ueber den wissenschaftlichen werth und die bedeutsamkeit der in den Ostseeländern vorkommenden arabischen münzen.

Mode of access: Internet.

Epistolae secretae ad principem suum Augustum ...

At head of title: Arcana seculi decimi sexti Huberti Langueti ...

Singularia iuris publici Germanici Imperii...

Mode of access: Internet.

Tradução do conto de Peter Handke “Das Umfallen der Kegel von einer bäuerlichen Kegelbahn”

O conto aqui traduzido é um exemplo acabado de desconstrucionismo, sobretudo conseguido através da menorização do papel do narrador, constantemente desautorizado pelas personagens, que o interrompem e o contradizem. Assim, alguns segmentos aparentemente incongruentes não o são de facto. Na tarefa de tradução deste conto, mantivemos as características de descontrução do texto de partida, pelo que quaisquer aparentes incoerências são inerentes ao texto original e não erros de tradução.

Reise nach den Inseln Teneriffa, Trinidad, St. Thomas, St. Crux und Porto-Rico; auf Befehl der französischen Regierung, vom 30. Sept. 1796 bis zum 7. Juni 1789, unter der Leitung des Capitain Baudin unternommen, von Peter Le Dru. Mit Bemerkungen begleitet, nebst einer allgemeinen Ubersicht des ganzen westindischen Archipels, vorzüglich in Rücksicht der Kolonialwaaren, von E. A. W. Zimmermann.

Bd.1 (1811)