Cyclic AMP production and insulin releasing activity of synthetic fragment peptides of glucose-dependent insulinotropic polypeptide

Synthetic fragment peptides of glucose-dependent insulinotropic polypeptide (GIP) were evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. In GIP receptor transfected CHL cells, GIP(4-42) and GIP(17-30) dose-dependently inhibited GIP-stimulated cAMP production (40 +/- 8%; p <0.01 and 15 +/- 6%; p <0.05, respectively), while GIP(1-16) exerted very weak agonist effects on cAMP production. In the clonal pancreatic beta-cell line, BRIN-BD11, GIP(1-16) demonstrated weak insulin releasing activity compared with native GIP. In contrast, GIP(4-42) and GIP (17-30) weakly antagonized the insulin releasing activity of the native peptide (23 +/- 6%; p <0.05 and 11 +/- 3%, respectively). These data demonstrate the critical role of the N-terminus and the involvement of regions of the C-terminal domain in generating full biological potency of GIP.

The status of Nathusius' pipistrelle (Pipistrellus nathusii Keyserling &amp; Blasius, 1839) in the British Isles

There are now 91 records of individual Nathusius' pipistrelle Pipistrellus nathusii examined 'in the hand' in the U.K., and bat detector records from a further 36 localities in Britain and Ireland. These records are distributed throughout all months of the year with clear peaks in spring and autumn suggesting migratory movements into and out of the British Isles in autumn and spring, respectively. Three maternity colonies have been located and the species must now also be regarded as a resident breeding species in Britain and Ireland.