983 resultados para 678.01


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利用多信息量测试系统分别测试了反射式GaAs光电阴极激活后在0(无光照),33和100 lx白光照射情况下阴极的光电流衰减变化曲线,计算得到其寿命分别为320,160和75 min,阴极稳定性随光照强度的增加而降低,测试了只有光照(100 lx)而无光电流流过阴极时阴极的寿命为100 min.通过比较发现光照比光电流对阴极稳定性的影响更大.还测试了阴极在33 lx光照下量子效率曲线随时间的衰减,发现阴极低能光子的量子效率下降速度更快,导致量子效率曲线形状不断发生变化.基于修正后的反射式阴极量子效率公式对这种变化进行了理论分析,发现与光电子的谷间散射和阴极衰减过程中表面势垒形状的变化有关.

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为研究氮化硅薄膜发光材料的制备工艺及其光致发光机制,实验采用射频磁控反应溅射技术与热退火处理制备了纳米硅镶嵌氮化硅薄膜材料.利用红外光谱(IR)、X射线衍射谱(XRD)、能谱(EDS)和光致发光谱(PL),对不同工艺条件下薄膜样品的成分、结构和发光特性进行研究,发现在制备的富硅氮化硅薄膜材料中形成了纳米硅颗粒,并计算出其平均尺寸.在510 nm光激发下,观察到纳米硅发光峰,对样品发光机制进行了讨论,认为其较强的发光起因于缺陷态和纳米硅发光.

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应用多种光谱手段研究了分子束外延生长在半绝缘的(001,GaAs衬底上的低氮含量的GaNAs中三元合金态的光学特性.变温PL谱揭示了合金态的本征特性以及其与氮的杂质态的根本区别,而脉冲激发的光荧光谱则进一步显示了合金态的本征光学特性.最后还研究了GaNAs的吸收光谱特征.

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本文论述用于兰州重离子加速器冷却存储环(HIRFL-CSR)控制系统的前端总线系统控制器FBC-01的硬件设计。该控制器是基于0.8mmBGA封装的AT91RM9200(ARM9)处理器,运行嵌入式LINUX操作系统。控制器可以连接标准的VGA显示器、键盘、鼠标,具有通用的10M/100M以太网接口、USB接口、RS-232接口、485接口、CANBUS接口。可以带SD卡、CF卡存储器。该控制器采用现场可编程的FPGA器件设计背板接口,并采用具有64mA高驱动能力的总线驱动器,不仅符合VME规范的电气要求,而且具有灵活的接口信号定义可编程能力,是HIRFL-CSR控制系统的关键部件。

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采用XRD、FESEM-EDS、ICP及EIS等方法研究了Ti0.17Zr0.08V0.34Nb0.01Cr0.1Ni0.3氢化物电极合金微观结构和电化学性能。X射线衍射分析表明:该合金由体心立方结构(bcc)的V基固溶体主相和少量六方结构的C14型Laves相组成;FESEM及EDS分析表明:V基固溶体主相形成树枝晶,C14型Laves相呈网格状围绕着树枝晶的晶界,元素在两相中的分布呈现镜像关系。电化学性能测试结果表明:该合金的氢化物电极在303 ̄343K较宽的温度区间内,表现出较高的电化学容量,在303K和343K时,电化学容量分别为337.0mAh·g-1和327.9mAh·g-1。在303K循环100周后,容量为282.7mAh·g-1。ICP分析结果表明,氢化物电极在充放电循环过程中,V及Zr元素向KOH电解质中的溶出较为严重。EIS研究表明,金属氢化物电极表面电化学反应的电荷转移电阻(RT)随循环次数的增加而增加,相应的交换电流密度则随循环次数的增加而降低。氢化物电极循环过程中RT的增大以及V和Zr元素的溶解,可能是导致电极容量衰减的主要原因。

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介绍了GH -0 1双组分、室温固化环氧树脂超低温胶粘剂的某些性能 ,说明这种胶粘剂能粘接多种材料 ,尤其是在液氮温度下仍有良好的粘接性能 ,并且真空放气量小 ,是在超低温环境下使用的良好胶粘剂

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本文报导了用于热缩电缆附件中间连接的一种新型填充段电应力疏散胶的配方,研究了电性能调节剂,增粘剂,填料,氨酸橡胶等对产品性能的影响.

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LPS-induced TNF-alpha factor (LITAF) is a novel transcriptional factor that was first discovered in LPS-stimulated human macrophage cell line THP-1. LITAF can bind to TNF-a promoter to regulate its expression. The first scallop LITAF (named as CfLITAF) was cloned from Zhikong scallop Chlamys farreri by Expressed Sequence Tag (EST) and Polymerase Chain Reaction (PCR) techniques. The cDNA of CfLITAF was of 1240 bp and consisted of a 5' untranslated region (UTR) of 112 bp, a 3' UTR of 678 bp and an open reading frame (ORF) of 450 bp encoding a polypeptide of 149 amino acids with an estimated molecular mass of 16.08 kDa and theoretical isoelectric point of 6.77. A typical conserved LITAF-domain was identified in CfLITAF by SMART analysis. Homology analysis of the deduced amino acid sequence of CfLITAF with other known sequences by using the BLAST program revealed that CfLITAF was homologous to the LITAF from human and rat (Identity = 46%), cattle, horse, mouse and chicken (Identity = 48%), western clawed frog (Identity = 42%), and zebrafish (Identity = 50%). The mRNA expression of CfLITAF in different tissues including haemocytes, muscle, mantle, heart, gill and gonad, and the temporal expression in haemocytes challenged by LPS or peptidoglycan (PGN) were measured by Real-time RT-PCR. CfLITAF mRNA transcripts could be detected in all tissues examined and be up-regulated in haemocytes after LPS challenge. No significant changes were observed after PGN stimulation. All these data indicated the existence of LITAF in scallop and also provided clue on the presence of TNF-alpha-like molecules in invertebrates. (C) 2007 Elsevier Ltd. All rights reserved.

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海湾扇贝Argopecten irradian Lamarck于1982年从美国引种到中国,由于具有较快的生长速度和很高的经济效益,海湾扇贝成为中国最主要的养殖贝类之一。近年来海湾扇贝养殖遇到了死亡率高等问题,深入开展海湾扇贝功能基因的研究,尤其是免疫相关基因及其机制研究并在此基础上寻找扇贝疾病防治的有效方法对海湾扇贝的健康养殖十分重要。 对于贝类免疫系统来说,其血细胞在先天性免疫防御中起着重要的作用。当受到外界病原侵染时,贝类血细胞的一个重要免疫反应就是吞噬作用。在吞噬病原过程中,受到病原侵染的贝类还会产生其他多种免疫反应,这些免疫反应将消耗大量的能量(ATP),产能的呼吸链会加速运转,由此也会引发与呼吸链相耦联的活性氧(ROS)的大量产生。这些活性氧具有极强的反应特性,能破坏病原微生物的结构和功能分子,实现对入侵病原的杀灭。利用活性氧对被吞噬的病原进行杀灭,这是吞噬作用消除病原抵御侵染的重要机制。但由于活性氧分子反应的非特异性,它们也会破坏宿主机体细胞内的功能蛋白分子、不饱和脂肪酸分子和核酸等,对细胞造成严重的伤害,进而导致机体生理机能的损伤和免疫系统的破坏。所以,及时消除病原感染机体内过量产生的ROS,维持相关细胞的正常代谢,对提高机体抵抗力和免疫力具有重要的作用。O2-是生物体内产生的第一种活性氧分子,其他的活性氧分子也是由它衍生而来,消除过量O2-是消除过量活性氧危害的第一步也是关键一步。生物体内,超氧化物歧化酶(SOD)是催化O2-发生歧化反应,消除O2-的关键酶。 首先,本文通过RACE方法获得了海湾扇贝SOD家族全部三种基因的cDNA全长并对其进行了序列的生物信息学分析,海湾扇贝AiCuZnSOD全长cDNA为1047个碱基,其中开放阅读框为459个碱基,编码152个氨基酸,与栉孔扇贝Chlamys farreri的CuZnSOD相似度为77.5%,与长牡蛎Crassostrea gigas的相似度为75%,与人的相似度为74.7%。AiMnSOD全长cDNA为1207个碱基,其中开放阅读框为678个碱基,编码226个氨基酸,序列比对结果发现AiMnSOD的氨基酸序列与虾夷扇贝Mizuhopecten yessoensis和皱纹盘鲍Haliotis discus hannai的相似度分别为85%和78.4%,与哺乳动物相似度也在68%~72%之间。AiECSOD全长cDNA为893个碱基,其中开放阅读框为657个碱基,编码218个氨基酸。AiECSOD与其它物种ECSOD相似度比较低。与线虫Brugia pahangi的相似度为27.9%,与疟蚊Anopheles gambiae的相似度为31.4%,与斑马鱼Danio rerio的相似度为27.8%,与人的相似度也只有28.6%,与同是贝类的长牡蛎ECSOD也只有28.1%的相似性。主要原因是AiECSOD的信号肽和肝磷脂结合区域在各物种中无同源性。 其次,采用qRT-PCR(quantitative real time PCR)方法分析三种SOD基因在不同组织中的表达情况,结果表明三种SOD基因的组织表达有所差异。AiCuZnSOD基因在鳃中表达水平最高,其次是血细胞和性腺,在外套膜、闭壳肌和肝胰脏表达水平较低。AiMnSOD基因在鳃中表达水平最高,其次是外套膜,在血细胞、性腺,而在肝胰脏和闭壳肌表达较弱。AiECSOD基因在血细胞中表达水平最高,其次是肝胰脏,在鳃、闭壳肌表达水平较低,而性腺和外套膜没有检测到。同时,采用qRT-PCR对鳗弧菌Vibrio angullarum感染后海湾扇贝血细胞中三种SOD基因mRNA表达变化进行了检测。AiCuZnSOD表达量在各个时间段没有显著差异(P > 0.05)。AiMnSOD的表达量在1.5 h时略有下降,在3 h时达到最高表达量,是空白组(0h)的3倍(P < 0.01),从6 h到24 h表达量逐渐下降,24 h时表达量是空白组的1.6倍,24 h到48 h又稍有升高。AiECSOD的表达量在1.5 h时有所下降,是空白组的0.3倍(P < 0.05),随后逐渐升高,在12 h时达到最高表达量,是空白组(0h)的4.5倍(P < 0.01),从24 h到48 h表达量逐渐下降并恢复到空白组的水平。在对照组,各个时间点没有显著差异(P > 0.05)。在鳗弧菌感染后,海湾扇贝三种SOD的表达并不一致,且差异比较显著。AiCuZnSOD被认为是构成性表达基因,其受外界刺激的影响最小,AiMnSOD和AiECSOD受刺激后表达上调比较明显。 第三,采用Genome-walking的方法得到了海湾扇贝三种SOD基因的基因组全长和近端启动子序列并对其进行了相关分析。AiCuZnSOD的基因组序列全长为4279bp,包含有4个外显子和3个内含子。AiMnSOD的基因组序列全长为10692bp,包含有4个外显子和3个内含子。AiECSOD的基因组序列全长为5276bp,包含有5个外显子和4个内含子。三种基因外显子和内含子的结合处序列遵循-AT/GT-原则。我们把海湾扇贝SOD家族的三个基因的近端启动子进行了比较分析。发现三种SOD在靠近起始密码子的位置都有Oct-1结合位点。三种SOD共有的转录位点有:Oct-1、C/EBPalp、Oct2.1、Sp-1和GATA-1。AiCuZnSOD和AiMnSOD共有的转录位点有:ICSBP、Ftz、TATA-box、C/EBPbeta和Antp。AiCuZnSOD和AiECSOD共有的转录位点有:AP-1和NFκB。AiMnSOD和AiECSOD共有的转录位点有:GR和ER。AiCuZnSOD独有的位点有:SRF、YY-1和NF-1。AiMnSOD独有的位点有:HNF-1、Hb、MEB、NF-muE1、Pit-1a和Eve。AiECSOD独有的位点有:CREB、RATA-alph、Kruppel-like和AP-3。 此外,通过构建原核表达载体,本研究对AiCuZnSOD和AiECSOD基因进行了体外重组表达,并对纯化的重组蛋白进行了酶活分析。酶活分析表明,重组AiCuZnSOD蛋白有较高的酶活和稳定性。 最后,我们对海湾扇贝三种SOD基因的部分区域,包括启动子、编码区,部分内含子区域进行了SNP检测,并对SOD基因部分SNP位点多态性和鳗弧菌敏感性进行了相关分析。三种SOD基因中,我们共发现了59个SNP位点,其中AiECSOD的SNP位点最多,特别是在启动子区,AiCuZnSOD和AiMnSOD多态性较低。其中AiCuZnSOD启动子区的-1739 T-C 位点的基因型和等位基因,AiECSOD启动子区的-498 A-T和-267 G-A等位基因频率,AiECSOD的第一个外显子38 Thr-Lys的多态性在敏感和抗菌群体中存在显著差异(P < 0.05)。

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A novel manganese superoxide dismutase (MnSOD) was cloned from bay scallop Argopecten irradians by 3' and 5' rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of MnSOD was of 1207 bp with a 678 bp open reading frame encoding 226 amino acids. The deduced amino acid sequence contained a putative signal peptide of 26 amino acids. Sequence comparison showed that the MnSOD of A. irradians shared high identity with MnSOD in invertebrates and vertebrates, such as MnSOD from abalone Haliotis discus discus (ABG88843) and frog Xenopus laevis (AAQ63483). Furthermore, the 3D structure of bay scallop MnSOD was predicted by SWISS-MODEL Protein Modelling Server and compared with those of other MnSODs. The overall structure of bay scallop MnSOD was similar to those of zebrafish Danio rerio, fruit fly Drosophila melanogaster, Chinese shrimp Fenneropenaeus chinensis, human Homo sapiens, and had the highest similarity to scallop Mizuhopecten yessoensis and abalone H. discus discus. A quantitative real-time PCR (qRT-PCR) assay was developed to detect the mRNA expression of MnSOD in different tissues and the temporal expression in haemocytes following challenge with the bacterium Vibrio anguillarum. A higher-level of mRNA expression of MnSOD was detected in gill and mantle. The expression of MnSOD reached the highest level at 3 h post-injection with V. anguillarum and then slightly recovered from 6 to 48 h. The results indicated that bay scallop MnSOD was a constitutive and inducible protein and thus could play an important role in the immune responses against V anguillarum infection. (c) 2008 Elsevier Ltd. All rights reserved.

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O Município de Machadinho d´Oeste, em Rondônia foi escolhido para um estudo de longo prazo. Já são 10 anos de acompanhamento de mais de 450 pequenas propriedades rurais e cerca de 250 variáveis biofísicas, sócio-econômicas e ambientais. A duração deste projeto é de 100 anos. Os estudos pedológicos da área do "Projeto de Assentamento Machadinho" teve como objetivos identificar os solos das Glebas e implementar uma base de dados do meio físico em escala municipal, através do uso de técnicas de geoprocessamento (SIG´s); bem com o caracterizar e avaliar química, física e morfologicamente os perfis de solos representativos das principais unidades identificadas, a fim de promover o desenvolvimento agrícola da região e fornecer subsídios para estudos em áreas semelhantes na Amazônia.

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Identificacao dos sintomas de fitotoxicidade; Decisao quanto a semeadura; Importancia da manutencao da tecnologia do tratamentodesementes de soja com fungicidas; Outros problemas que tem ocorrido simultaneamente nas lavouras.