Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168

Cellulases participate in a number of biological events, such as plant cell wall remodelling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited by both their low catalytic efficiency and stability. In the present study, we conducted a detailed functional and structural characterization of the thermostable BsCe15A (Bacillus subtilis cellulase 5A), which consists of a GH5 (glycoside hydrolase 5) catalytic domain fused to a CBM3 (family 3 carbohydrate-binding module). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif, and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion was demonstrated to have a hyper-stabilizing effect on BsCel5A, and by using deletion constructs and X-ray crystallography we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.

Apical surgery: endoscopic findings at the resection level of 168 consecutively treated roots

Endoscopic evaluation of the cut root face after root-end resection during apical surgery.

Ms. hebr. oct. 168 - Kerakh Frankfurt : Skizzen zur Geschichte der Juden in Frankfurt am Main

Vorbesitzer: Eljāqīm Carmoly

Cross-sectional study of 168 patients with hepatorenal tyrosinaemia and implications for clinical practice

BackgroundHepatorenal tyrosinaemia (Tyr 1) is a rare inborn error of tyrosine metabolism. Without treatment, patients are at high risk of developing acute liver failure, renal dysfunction and in the long run hepatocellular carcinoma. The aim of our study was to collect cross-sectional data.MethodsVia questionnaires we collected retrospective data of 168 patients with Tyr 1 from 21 centres (Europe, Turkey and Israel) about diagnosis, treatment, monitoring and outcome. In a subsequent consensus workshop, we discussed data and clinical implications.ResultsEarly treatment by NTBC accompanied by diet is essential to prevent serious complications such as liver failure, hepatocellular carcinoma and renal disease. As patients may remain initially asymptomatic or develop uncharacteristic clinical symptoms in the first months of life newborn mass screening using succinylacetone (SA) as a screening parameter in dried blood is mandatory for early diagnosis. NTBC-treatment has to be combined with natural protein restriction supplemented with essential amino acids. NTBC dosage should be reduced to the minimal dose allowing metabolic control, once daily dosing may be an option in older children and adults in order to increase compliance. Metabolic control is judged by SA (below detection limit) in dried blood or urine, plasma tyrosine (<400 ¿M) and NTBC-levels in the therapeutic range (20¿40 ¿M). Side effects of NTBC are mild and often transient.Indications for liver transplantation are hepatocellular carcinoma or failure to respond to NTBC. Follow-up procedures should include liver and kidney function tests, tumor markers and imaging, ophthalmological examination, blood count, psychomotor and intelligence testing as well as therapeutic monitoring (SA, tyrosine, NTBC in blood).ConclusionBased on the data from 21 centres treating 168 patients we were able to characterize current practice and clinical experience in Tyr 1. This information could form the basis for clinical practice recommendations, however further prospective data are required to underpin some of the recommendations.

Die hebräische Presse im Jahre 1912 : (Fortsetzung von Seite 167/168)

Josef Lin

Ms. Barth. 168 - Theologische Sammelhandschrift

Vorbesitzer: Brune-Kütze-Legat; Bartholomaeusstift Frankfurt am Main

Ms. Praed. 168 - Funiculus triplex

Vorbesitzer: Dominikanerkloster Frankfurt am Main

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 168 - Nachdrückliche Bitte von Herrn Dr. Kastner um Verschwiegenheit wegen des vorausgegangenen Bittschreibens

Abschrift liegt vor

Ms.Ff.F.Stoltze 3. 168 - Brief von Theobald Kerner an Friedrich Stoltze

Beschwerde wegen der Anrede "Hofrath", Übersendung eines Textes für einen Autographensammler, Übersendung eines Rings für Molly Stoltze zur Verlobung, Else Körner

Na 1 Nachlass Max Horkheimer, 446 - Korrespondenzen mit der UNESCO (p. V 168, 145-206)

Drucksachen und Schriftwechsel der UNESCO (Paris, Hamburg, Gauting), 1957-1965; 69 Briefe zwischen Dr. Gilda Mara, UNESCO und Max Horkheimer, 1959-1965; 12 Briefe zwischen Dr. Helga Timm, UNESCO und Max Horkheimer, 1957-1958;

Na 1 Nachlass Max Horkheimer, 520 - Korrespondenzen mit Walter Benjamin (Kopien) (p. VI 5A, 168 - 325)

4 Briefe zwischen Walter Benjamin und Cecilia Razovsky; 6 Briefe zwischen Cecilia Razovsky und Max Horkheimer; 1 Brief von Cecilia Razovsky an den American Consul, 17.11.1939; 1 Brief von Antonio Sols an Max Horkheimer, 30.10.1940; 1 Brief von Max Horkheimer an Antonio Sols, 11.10.1940; 1 Brief (Abschrift, Auszug) an A. R. L. Gurland von Frau Gurland, 11.10.1940; 3 Briefe zwischen dem Emergency Rescue Committee (New York) und Max Horkheimer, August 1940; 1 Brief von Max Horkheimer an Pastor del Rio, 17.07.1940; 1 Brief von Max Horkheimer an J. Wavrinek, 17.07.1940; 1 Brief von Theodor W. Adorno an Gershom Scholem, 16.07.1940; 1 Brief von Max Horkheimer an Hans Schaudinger, 13.07.1940; 1 Brief von Max Horkheimer an George S. Messersmith, 08.07.1940; 1 Brief von Max Horkheimer an Adolph Kates, 05.07.1940; 1 Brief von Max Horkheimer an Adolph Kates, 29.06.1940; 2 Briefe zwischen dem Refugee Section of the American Friends Service Committee (Philadelphia) und Max Horkheimer, Mai 1940; 2 Briefe von Egon Wissing an Max Horkheimer, 1940; 1 Brief von Max Horkheimer an die Economy Blue Print Co. (New York), 25.05.1940; 1 Brief von Werner Kraft an Max Horkheimer, 30.04.1940;

Über den altjüdischen Kalender, zunächst in seiner Beziehung zur neutestamentlichen Geschichte : eine kronologisch-kritische Untersuchung (zugleich ein Beitrag zur Evangelien-Harmonistik), nebst einem Anhang von Tafeln zur bequemen Berechnung altjüdischer Daten für den Zeitraum der Jahre 168 vor bis 72 nach Kr.

von Johannes von Gumpach

Na 1 Nachlass Max Horkheimer, 693 - "German Economy, Politics and Culture, 1900-1933" (p. IX 168 1.a)-d))

"German Economy, Politics and Culture, 1900-1933. A Research Project of the International Institute of Social Research" (1940):; 1. Umriß des Forschungsprojekts, a) Fassung vom 29.7.1940, Typoskript, 55 Blatt, b)-d) Fassung vom 6.6.1940: b) Typoskript, 55 Blatt, c) Typoskript mit handschriftlichen Korrekturen, 60 Blatt, d) Typoskript, 60 Blatt;

Ms. lat. oct. 168 - Quaestiones et disputationes theologicae

Vorbesitzer: Bernhard Waldschmidt;