Symmetrical 1-pyrrolidineacetamide showing anti-HIV activity through a new binding site on HIV-1 integrase

Aim: To characterize the functional and pharmacological features of a symmetrical 1-pyrrolidineacetamide, N,N'-(methylene-di-4,1-phenylene) bis-1-pyrrolidineacetamide, as a new anti-HIV compound which could competitively inhibit HIV-1 integrase (IN) bindi

雌性猕猴尿中生殖激素的变化

　用人血清激素放射免疫测定试剂盒,测定了4 只未孕雌性猕猴8 个月经周期尿中促黄体生成素 (LH) 、孕酮(P) 和雌二醇( E2) 的含量,结合对猕猴繁殖行为学的观察,研究了各激素分泌之间的关系及其与繁殖行 为的内在联系。结果表明:4 只雌性猕猴8 个月经周期的平均长度为(26. 25 ±2. 10) d ,行经时间(2. 25 ±0. 66) d , LH 峰值出现在(12. 75 ±0. 91) d ,平均峰值为(86. 30 ±20. 11) miu/ mL ;P 峰值出现在(19. 75 ±1. 87) d ,平均峰值为 (7. 47 ±1. 78) ng/ mL ;E2 峰值出现在(11. 75 ±1. 81) d ,平均峰值为(679. 11 ±90. 66) pg/ mL 。研究表明,人血清激 素放射免疫测定试剂盒可以测定出猕猴尿中生殖激素的变化趋势;LH、P、E2 的变化情况基本上能反映出猕猴月 经周期中卵泡形成、排卵及黄体形成的时间。

Characteristics of the microbial communities in the integrated vertical-flow constructed wetlands

Microorganisms play an important role in removing pollutants from constructed wetlands. We investigated the microbial characteristics in a novel integrated vertical-flow constructed wetland (IVCW), which has been in operation in Wuhan, China since 1998. We used phospholipid fatty acid (PLFA) and amoA gene to analyze the structure and diversity of the microbial community within the IVCW. PLFA results suggested that the amount of bacterial PLFA was significantly higher than that of fungal PLFA, but the total microbial biomass represented by PLFA index was low in the system. Microbial spatial distribution showed significantly higher bacterial (both G(+) and G(-)) and fungal biomass in the surface than in the subsurface layers. The ratios of monounsaturated to branched PLFA demonstrated that an anaerobic layer sandwiched by two aerobic layers existed in the IVCW, consistent with the redox potential results. Analysis of the amoA revealed the presence of Nitrosomonas-like sequences in the surface substrate of the downflow chamber and apparent diversities of ammonia-oxidizing bacteria in the system. These results suggest that microorganisms, despite their relatively low biomass, have inhabited the IVCW, and the results will offer some valuable information on microbe to system designers and managers.

Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis

Fifteen polymorphic microsatellite loci were developed for the Chinese soft-shelled turtle (Pelodiscus sinensis) from the (GT)(n) microsatellite-enriched genomic library, using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. The polymorphism of all 15 loci ranged from two to seven alleles with observed heterozygosities ranging from 0.03 to 0.98 (mean 0.43) in one population of 40 individuals. These novel loci will be helpful for understanding the population structure at genetic level and marker-assisted breeding of this vulnerable species.

Identification of a gene, ccr-1 (sll1242), required for chill-light tolerance and growth at 15 degrees C in Synechocystis sp PCC 6803

Synechocystis sp. PCC 6803 exposed to chill (5 degrees C)-light (100 mu mol photons m(-2) s(-1)) stress loses its ability to reinitiate growth. From a random insertion mutant library of Synechocystis sp. PCC 6803, a sll1242 mutant showing increased sensitivity to chill plus light was isolated. Mutant reconstruction and complementation with the wild-type gene confirmed the role of sll1242 in maintaining chill-light tolerance. At 15 degrees C, the autotrophic and mixotrophic growth of the mutant were both inhibited, paralleled by decreased photosynthetic activity. The expression of sll1242 was upregulated in Synechocystis sp. PCC 6803 after transfer from 30 to 15 degrees C at a photosynthetic photon flux density of 30 mu mol photons m(-2) S-1. sll1242, named ccr (cyanobacterial cold resistance gene)- 1, may be required for cold acclimation of cyanobacteria in light.

在挪威研究波浪能

在挪威研究波浪能余志（中国科学院广州能源研究所510070）1引言应挪威理工学院（NTH）J.Falnes教授的邀请并得到挪威研究基金会（NFR）和国家自然科学基金会的资助，我于1994年5月10日至11月3日在NTH物理系进行了为期六个月的访问与...

改进有限单元法动力特性方法的探讨

采样定理告诉人们进行离散逼近离散尺寸的极限是问题中所涉及最高频率波长的1/2.1/2波长是一理想值,在目前的有限元分析中,通常保证精度的空间离散尺寸远小于这一理想值.文中分析了这一现象的可能原因,同时给出了一种基于有限元的在一定条件下能够改进空间离散效率的动力模拟方法