Temporal Analysis of Lipopolysaccharide-Induced Sickness Behavior in Virgin and Lactating Female Rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Leg Amputation for an Extensive, Severe and Intractable Sickle Cell Anemia Ulcer in a Brazilian Patient

A 35-year-old African Brazilian patient had sickle cell anemia complicated with recurrent vasoocclusive (VOC) crises and refractory painful leg ulcers for 16 years. The ulcers started over both medial malleoli and expanded gradually. The ulcer on the left leg spread from the foot to the knee circumferentially and was refractory to all forms of therapy within the frame work of multi-disciplinary care. The patient agreed to a below the knee amputation of the left leg. He felt much better after the amputation but developed severe neuropathic phantom pain that was well controlled medically. He could differentiate the sickle cell anemia and ulcer pain from the neuropathic pain. About 6 months after the amputation he had dengue fever with fatal outcome. This is the first report of treatment of refractory sickle cell anemia leg ulcer with amputation and probably the first report of a Brazilian patient with sickle cell anemia and dengue fever.

A Saint Louis encephalitis and Rocio virus serosurvey in Brazilian horses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Streptococcal acute pharyngitis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

New drugs with antiprotozoal activity from marine algae: a review

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atividade larvicida dos óleos essenciais de Syzygium aromaticum e Citrus sinensis em populações de Aedes aegypti

Pós-graduação em Biociências - FCLAS

Acute respiratory infection and influenza-like illness viral etiologies in Brazilian adults

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A distribuição do dengue no estado de São Paulo e suas relações com o clima

This study linked the outbreak of dengue fever occurred in the state of São Paulo in 2007 and the sharp decline in cases reported in the year following the change of El Niño to La Niña. Therefore, raised temperature data for the months of January, February and March 2007 and 2008, recorded in 99 countie, which have allowed maps mean isotherms, maximum and minimum for each of the summers...

Estudo fotodinâmico da clorofila a, azul de metileno e rosa bengala como agente inseticida contra Aedes aegypti

Photodynamic therapy, term introduced by von Tapeiner in 1900, can be defined as the administration of a non toxic drug, i.e., a dye, known as photosensitizer (FS), which subsequently will be illuminated with light of specific wavelength. PDT is based on the interaction among FS, oxygen and light, which through photochemical reactions cause cell death. The FS molecules must have a high probability to form the singlet state after the excitation, which can induce chemical changes in the neighborhood in two ways, called reactions type I and type II. The type II reaction is based on the exchange of energy to molecular oxygen, exciting it to its state of higher energy (singlet), which is highly reactive. The proposed mechanisms for cell death are linked to damage to the DNA, mitochondria and to the cytoplasmic membrane. Several pre‐clinical and clinical trials have been carried out and the PDT is already used in many countries for treatment mainly against certain types of cancer. The therapy also has been gaining strength in antimicrobial control, since the microorganisms have appeared increasingly resistant to current antibiotics. Another attempt to use the PDT is for the inactivation of macro‐organisms, such as micro‐crustaceans and mosquitoes. To this end I tested whether the photosensitizers methylene blue, rose Bengal and the chlorophyll a has insecticidal activity against the yellow fever and dengue vector mosquito, Aedes aegypti. Since these diseases have no effective treatments, its control is linked to the vector control, which has shown resistance to chemical pesticides used. Based on this, this work shows its importance, because it is a new type of mosquito control since all the photosensitizers used are low cost, do not generate toxic products at the concentrations used and showed good results in mortality. The best photosensitizer was rose Bengal... (Complete abstract click electronic access below)

Endocardite infecciosa em pequenos animais

Infective endocarditis is a process in which an infection attacks the heart endothelial surface, and is commonly caused by bacterial colonization, which is called bacterial endocarditis. It is a condition rarely found in dogs and cats, and is more prevalent in male dogs of large size. It mainly affects the left side of the heart, affecting the mitral and aortic valves with greater frequency. The circulation of the bacterium in the bloodstream is what gives rise endocarditis, and is caused by any non-aseptic process that serves as a gateway for bacterium in the body, as from a skin lesion, even as an invasive procedure, such as, catheterization and surgery. The ante-mortem diagnosis is difficult because the clinical signs of endocarditis are varied and common to other diseases, summing up the signs of infection (fever, lethargy, weight loss), and presence of heart murmur and may show signs of congestive heart failure. Thus, the diagnosis is most often through autopsy. To arrive at a diagnosis should be used, besides the history and physical examination, some laboratory tests, especially blood cultures and echocardiography. Treatment is accomplished through the use of antibiotics for long period of time, it is very important to follow the results of susceptibility after its outcome is revealed. The prognosis for bacterial endocarditis ranges from guarded to poor, and can be assessed mainly by the echocardiography. There are few studies in veterinary about the bacterial endocarditis, and the majority is case reports

Antidepressivo em ratos com insuficiência aórtica pode modificar a expressão gênica de ocitocina e vasopressina centrais?

Aortic regurgitation (AR) leads to a left ventricle dilation and hypertrophy in response to a chronic volume overload. It is still very frequent in developing countries, for instance Brazil, and often as secondary to rheumatic fever. Usually, chronic AR is generally well tolerated for many years, when with the heart dilated the patient searches for treatment. Bidirectional association with depression and cardiovascular disease has been described. Selective serotonin reuptake inhibitors (SSRI) are widely prescribed to treat several affective disorders, especially for cardiovascular patients since they decrease arrhythmia probability. These SSRI improves cardiac function in rats submitted to stress protocols. Preliminary study from our laboratory showed that following 4 weeks of treatment with one SSRI (paroxetine) in subchronic AR rats there was a decreased in daily sodium intake and an improvement in systolic function. An increase in the central oxytocinergic transmission may be involved in this peripheral improvement to the heart. The investigations about the mechanisms underlying this improvement are necessary. Therefore the aims of this project is investigate the effects of 4 weeks of treatment of paroxetine, a SSRI, in rats with a subchronic AR over the central central gene expression of oxytocin and vasopressin using a reverse transcription polymerase chain reaction (RT-PCR)

Efeito de temperaturas alternadas sobre desenvolvimento de Aedes (Stegomyia) aegyoti (L.) (Diptera: Culicidae)

Aedes (Stegomyia) aegypti is a species of mosquitoes known to be the vector of diseases such as dengue and yellow fever, and a better understanding of aspects of their biology can help in the establishment of control strategies for the same. Several previous studies showed that temperature significantly affects the development of immature stages of insects. In general, higher temperatures (up to a threshold) accelerate the development of insects, and lower temperature retards the same. This rule also applies to mosquitoes, including Ae. aegypti. But not still know the effects of daily variation of temperature on the developmental stages of mosquitoes. And this detail is very important, since in natural breeding or artificial, The mosquitoes usually face temperature variations over a single day, which should interfere with its development until the emergence of the adult forms. For this reason, the objective of this study is to analyze the effect of alternating temperatures on the development of Ae. aegypti. To conduct the study, adults were collected active in the neighborhood Bela Vista Campus of UNESP - Rio Claro, SP, using a sweep net or using ovitraps for immatures, and the active search for breeding. Individuals collected were kept under experimental conditions in the laboratory. The adult samples were identified to species level, were considered for the experiments, only samples of Ae. aegypti. The insects were housed in plastic cages, suitable for creating flies. These were fed with sugar solution and blood meal on alternate days. The eggs obtained were used in the experiment with four different temperature regimes. The data collected were analyzed by evaluating whether the different treatments influenced the development of immature to adult, performing the Kruskal-Wallis test and the statistical software BioEstat. Statistical analysis of the sex ratio... (Complete abstract click electronic access below)

A dengue como ferramenta de interdisciplinaridade e popularização científica entre estudantes de ensino fundamental

The majority of the population knows their role in dengue control, preventing the accumulation of water in containers as potential breeding sites of Aedes aegypti, the main vector of dengue. Also known chemical control, widely used by the institutions responsible for the control of endemic diseases. On the other hand, unaware that an alternative measure such biological control through a microbial biopesticide, safe, harmless to health and the environment, can be adopted. Thus, this work reported for elementary school students, simply and clearly, the biological control of mosquitoes through bacterial biopesticide produced locally. Moreover, promoted the interdisciplinary teaching of science and mathematics through the data tabulation and charting on interviews conducted by students, about the level of knowledge of the population about dengue and other related variables.