Uncertainty Estimation for Performance Evaluation of a Confocal Microscope as Metrology Equipment

Both in industry and research, the quality control of micrometric manufactured parts is based on the measurement of parameters whose traceability is sometimes difficult to guarantee. In some of these parts, the confocal microscopy shows great aptitudes to characterize a measurand qualitatively and quantitatively. The confocal microscopy allows the acquisition of 2D and 3D images that are easily manipulated. Nowadays, this equipment is manufactured by many different brands, each of them claiming a resolution probably not in accord to their real performance. The Laser Center (Technical University of Madrid) has a confocal microscope to verify the dimensions of the micro mechanizing in their own research projects. The present study pretends to confirm that the magnitudes obtained are true and reliable. To achieve this, a methodology for confocal microscope calibration is proposed, as well as an experimental phase for dimensionally valuing the equipment by 4 different standard positions, with its seven magnifications and the six objective lenses that the equipment currently has, in the x–y and z axis. From the results the uncertainty will be estimated along with an effect analysis of the different magnifications in each of the objective lenses.

Metodología de análisis acústico de sitios arqueológicos de Mesoamérica

El presente trabajo busca llenar un vacío existente en cuanto a una metodología general en los estudios Arqueoacústicos para la zona Mesoamericana. El resultado mas importante de ésta tesis es la propuesta de un procedimiento y método para los cuales se detalla el conjunto de operaciones utilizadas en las mediciones particulares involucradas, parámetros y análisis relevantes (de los cuales destacan el decaimiento de energía, tiempos de reverberación, intensidades de sonido directo y total, claridad e inteligibilidad), tipos de simulaciones e incertidumbres y actividades técnicas propias de la Ingeniería Acústica que se proponen para la efectiva y útil integración de la dimensión sonora en el quehacer arqueológico. Una importante consideración de la propuesta metodológica aquí presentada, es la consideración, por un lado, de los resultados obtenidos experimentalmente del trabajo in situ en cada uno de los sitios arqueológicos de interés, y por otro los resultados obtenidos de las simulaciones y modelaciones por computadora de los mismos para su comparación y contraste. La propuesta de sistematización está dividida en tres momentos investigativos, como son la coordinación y trabajo logístico en sitios arqueológicos, su reconocimiento y prospección acústica; el trabajo de campo; y el análisis de los resultados obtenidos. Asimismo, dicha propuesta metodológica, se presenta dividida en tres objetos de estudio fundamentales en el quehacer arqueoacústico: los Fenómenos Sonoros encontrados en sitios arqueológicos; los objetos de generación de sonido, tengan éstos aplicaciones musicales o de otra índole; y finalmente los espacios y recintos arquitectónicos, ya sean cerrados o abiertos, y el estudio de su funcionalidad. El acercamiento a la descripción de cada una de dichas ramas, se realiza mediante la presentación de casos de estudio, para los que se contrastan dos áreas culturales representativas e interrelacionadas de Mesoamérica, la zona del Bajío y la zona Maya. Dicho contraste es llevado a cabo mediante el análisis acústico de 4 zonas arqueológicas que son Plazuelas, Peralta y Cañada de la Virgen, pertenecientes a la zona del Bajio, y Chichen Itzá perteneciente a la zona Maya. De particular importancia para el presente trabajo, es la funcionalidad de los espacios estudiados, específicamente de la de los patios hundidos, característicos de la arquitectura del Bajío, y las plazas públicas que constituyen una estructura integral en muchos sitios arqueológicos, centrales para la comprensión de las conexiones entre el fenómeno sonoro y el comportamiento de una cultura en particular. Asimismo, el análisis de los instrumentos de generación sonora ha permitido realizar inferencias sobre papel del sonido en el comportamiento humano de las culturas en cuestión, completando los modelos acústicos y posibilitando el situar las características de las fuentes sonoras en los espacios resonantes. Los resultados particulares de ésta tesis, han permitido establecer las características acústicas de dichos espacios e instrumentos, así como formular y validar las hipótesis sobre su usos y funciones como espacios para eventos públicos y sociales, así como para representaciones culturales multitudinarios. ABSTRACT The present work, aims to fill a void existing in terms of a general methodology in Archaeoacoustic studies for the Mesoamerican region. The most important result of this thesis is the proposal of a procedure and method for archaeoacoustical studies and its relevant analysis and parameters (of which stand out the energy decay, reverberation times, intensities, clarity and intelligibility), simulations and uncertainty considerations for the effective and useful integration of the sound dimension in the archaeological realm. An important methodological consideration in the present work is the comparison and contrast of the results obtained experimentally on site, and the results of simulations and computer modeling. The proposed systematization is divided into three distinct moments: logistics; fieldwork; and result analysis. Likewise, the presented methodological proposal is divided into three fundamental objects of study: Sound phenomena found in archaeological sites; sound generation objects and instruments; and architectural enclosures and the study of their functionality. Presenting case studies is the approach to the description of each of these branches. Two interrelated Mesoamerican cultural areas are considered, the Bajío and the Mayan. Their contrast is carried out by the acoustic analysis of four archaeological sites: Plazuelas, Peralta and Cañada de la Virgen, belonging to the area of the Bajío, and Chichen Itza belonging to the Maya area. Of particular relevance to this paper, is the acoustic functionality of the public spaces present in many archaeological sites, specifically that of the sunken patios, characteristic of the architecture of the Bajío. The analysis of the sound generation instruments has allowed certain inferences about the role of sound in human behavior of the cultures in question, thus completing the acoustic models. It has also enable the contrast of the characteristics of the sound sources and those of the resonant spaces. The results of this thesis have made it possible to develop and validate certain hypotheses about the uses and functions of certain spaces for public and social events as well as for mass cultural representations.

Diseño de didácticas digitales para la asignatura de sistemas operativos

In this paper the first stage of implementation of digital content is presented through a website, that includes OS issues. Currently students at the Technological University of the Valle of Toluca (UTVT) represents a high degree of learning difficulty in this area. Then academic staff develops this project, in collaboration with the Technological University of the Suroeste of Guanajuato (UTSOE), which aims to improve the teaching-learning and make teaching practices incorporating TIC provide students with a tool to promote their interest. Through the interactivity offered by the web, the student to achieve strengthening further their knowledge and actively participate, which has easy access to information, where distance and time is not a constraint, in addition to have an effective view of the concepts needed to acquire the skills and the ability to self-regulate their learning. For the development of project is used methodologies of the software engineering and technologies web for design.

Breakers of advanced glycation end products restore large artery properties in experimental diabetes

Glucose and other reducing sugars react with proteins by a nonenzymatic, posttranslational modification process called nonenzymatic glycation. The formation of advanced glycation end products (AGEs) on connective tissue and matrix components accounts largely for the increase in collagen crosslinking that accompanies normal aging and which occurs at an accelerated rate in diabetes, leading to an increase in arterial stiffness. A new class of AGE crosslink “breakers” reacts with and cleaves these covalent, AGE-derived protein crosslinks. Treatment of rats with streptozotocin-induced diabetes with the AGE-breaker ALT-711 for 1–3 weeks reversed the diabetes-induced increase of large artery stiffness as measured by systemic arterial compliance, aortic impedance, and carotid artery compliance and distensibility. These findings will have considerable implications for the treatment of patients with diabetes-related complications and aging.

Rapid ATM-dependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage

The p53 tumor-suppressor protein, a key regulator of cellular responses to genotoxic stress, is stabilized and activated after DNA damage. This process is associated with posttranslational modifications of p53, some of which are mediated by the ATM protein kinase. However, these modifications alone may not account in full for p53 stabilization. p53's stability and activity are negatively regulated by the oncoprotein MDM2, whose gene is activated by p53. Conceivably, p53 function may be modulated by modifications of MDM2 as well. We show here that after treatment of cells with ionizing radiation or a radiomimetic chemical, but not UV radiation, MDM2 is phosphorylated rapidly in an ATM-dependent manner. This phosphorylation is independent of p53 and the DNA-dependent protein kinase. Furthermore, MDM2 is directly phosphorylated by ATM in vitro. These findings suggest that in response to DNA strand breaks, ATM may promote p53 activity and stability by mediating simultaneous phosphorylation of both partners of the p53-MDM2 autoregulatory feedback loop.

Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin

Aldosterone and vasopressin are responsible for the final adjustment of sodium and water reabsorption in the kidney. In principal cells of the kidney cortical collecting duct (CCD), the integral response to aldosterone and the long-term functional effects of vasopressin depend on transcription. In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCDcl4) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. Limiting the analysis to tags that occurred twice or more in the data set, 14,654 different transcripts were identified, 3,642 of which do not match known mouse sequences. Statistical comparison (at P < 0.05 level) of the three SAGE libraries revealed 34 AITs (aldosterone-induced transcripts), 29 ARTs (aldosterone-repressed transcripts), 48 VITs (vasopressin-induced transcripts) and 11 VRTs (vasopressin-repressed transcripts). A selection of the differentially-expressed, hormone-specific transcripts (5 VITs, 2 AITs and 1 ART) has been validated in the mpkCCDcl4 cell line either by Northern blot hybridization or reverse transcription–PCR. The hepatocyte nuclear transcription factor HNF-3-α (VIT39), the receptor activity modifying protein RAMP3 (VIT48), and the glucocorticoid-induced leucine zipper protein (GILZ) (AIT28) are candidate proteins playing a role in physiological responses of this cell line to vasopressin and aldosterone.

Estrogen increases synaptic connectivity between single presynaptic inputs and multiple postsynaptic CA1 pyramidal cells: A serial electron-microscopic study

Dendritic spines are sites of the vast majority of excitatory synaptic input to hippocampal CA1 pyramidal cells. Estrogen has been shown to increase the density of dendritic spines on CA1 pyramidal cell dendrites in adult female rats. In parallel with increased spine density, estrogen has been shown also to increase the number of spine synapses formed with multiple synapse boutons (MSBs). These findings suggest that estrogen-induced dendritic spines form synaptic contacts with preexisting presynaptic boutons, transforming some previously single synapse boutons (SSBs) into MSBs. The goal of the current study was to determine whether estrogen-induced MSBs form multiple synapses with the same or different postsynaptic cells. To quantify same-cell vs. different-cell MSBs, we filled individual CA1 pyramidal cells with biocytin and serially reconstructed dendrites and dendritic spines of the labeled cells, as well as presynaptic boutons in synaptic contact with labeled and unlabeled (i.e., different-cell) spines. We found that the overwhelming majority of MSBs in estrogen-treated animals form synapses with more than one postsynaptic cell. Thus, in addition to increasing the density of excitatory synaptic input to individual CA1 pyramidal cells, estrogen also increases the divergence of input from individual presynaptic boutons to multiple postsynaptic CA1 pyramidal cells. These findings suggest the formation of new synaptic connections between previously unconnected hippocampal neurons.

Profilin I is essential for cell survival and cell division in early mouse development

Profilins are thought to play a central role in the regulation of de novo actin assembly by preventing spontaneous actin polymerization through the binding of actin monomers, and the adding of monomeric actin to the barbed actin-filament ends. Other cellular functions of profilin in membrane trafficking and lipid based signaling are also likely. Binding of profilins to signaling molecules such as Arp2/3 complex, Mena, VASP, N-WASP, dynamin I, and others, further implicates profilin and actin as regulators of diverse motile activities. In mouse, two profilins are expressed from two distinct genes. Profilin I is expressed at high levels in all tissues and throughout development, whereas profilin II is expressed in neuronal cells. To examine the function of profilin I in vivo, we generated a null profilin I (pfn1ko) allele in mice. Homozygous pfn1ko/ko mice are not viable. Pfn1ko/ko embryos died as early as the two-cell stage, and no pfn1ko/ko blastocysts were detectable. Adult pfn1ko/wt mice show a 50% reduction in profilin I expression with no apparent impairment of cell function. However, pfn1ko/wt embryos have reduced survival during embryogenesis compared with wild type. Although weakly expressed in early embryos, profilin II cannot compensate for lack of profilin I. Our results indicate that mouse profilin I is an essential protein that has dosage-dependent effects on cell division and survival during embryogenesis.