Reproduction of Meloidogyne enterolobii in lettuce cultivars of the american group

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Metodologias de aplicação de sulfonamida heterocíclica no controle de Meloidogyne javanica e Pratylenchus brachyurus em soja

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Inter-relações entre nematoides, fungo e a cultura da seringueira

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Helminth communities of Leptodactylus latrans (Anura: Leptodactylidae) from the Atlantic rainforest, south-eastern Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria

Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Menthe piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil (PMO) at sub-Minimum Inhibitory Concentrations (sub-MICs) strongly interfered with acyl homoserine lactone (AHL) regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the QS inhibitory activity exhibited by PMO to menthol. Assessment of ability of menthol to interfere with QS systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors, and biofilm formation indicating broad-spectrum anti-QS activity. Using two Escherichia colt biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs QS systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum QS inhibitor.

New record of Pelecitus sp (Nematoda, Onchocercidae) as a parasite of Athene cunicularia (Strigiformes, Strigidae) in southeastern Brazil

The aim of this study was to report the burrowing owl Athene cunicularia as a new host for the filarid nematode Pelecitus sp. in southeastern Brazil for the first time, as well as reporting the occurrence of this nematode species in the body cavity, near the cervical air sac and lung region. This study contributes towards knowledge of parasitism in Brazilian wild birds and an anatomical region of the host as an infection site for Pelecitus sp.

Resistência de porta-enxertos de cucurbitáceas a nematóides e compatibilidade da enxertia em melão

Nematodes severely attack net melon plants under protected cultivation conditions. The objective of this research was to select rootstocks with resistance to Meloidogyne incognita and M. javanica. The experiment was carried out under greenhouse conditions from October 2010 to April 2011 in Jaboticabal, Sao Paulo state, Brazil. Thirty-three cucurbitaceous genotypes were investigated as rootstocks; melons: CNPH 01-930 (Cucumis melo var. flexuosus), CNPH 01-962, 01-963 CNPH (Cucumis melo var. conomon), cvs. Gaucho Redondo, Gaucho Comprido, Redondo Amarelo, Gulfcoast, Chilton, Bonus no. 2, Fantasy; watermelons: cv. Charleston Gray, Progenie da Coreia (Citrullus lanatus); pumpkins: cvs. Mra. Ma, Ornamental, Howden, Mammoth, Kururu, Goianinha (Cucurbita moschata); gourd: Abobora de Porco, cvs. Maranhao, Brasileirinha (Lagenaria siceraria); squash: cv. Pataca Gigante (Cucurbita maxima); cucumber: cvs. Caipira, Branco Meio Comprido, Curumim (Cucumis sativus); loofah: Metro, Semente Branca, Semente Preta (Luffa cylindrica); wax gourd (Benincasa hispida); pumpkin rootstock: Hybrid cv. Keij; snake gourd (Trichosanthes cucumerins) and musk cucumber (Sicana odorifera). To evaluate the resistance, seedlings were transplanted to pots and the root inoculated with 3,000 eggs and second stage juveniles of M. incognita and M. javanica. Fifty days after the inoculation, the plants were evaluated for nematode resistance by means of the reproduction factor. The grafting compatibility between net melon cvs. Bonus no. 2 and Fantasy and the rootstocks previously characterized as resistant were evaluated by means of 60 graftings. CNPH 01-962, CNPH 01-963 and melon 'Gaucho Redondo', were considered resistant to M. incognita. Melon 'Redondo Amarelo', watermelon 'Charleston Gray', watermelon Progenie da Coreia, Trichosanthes cucumerins were considered resistant to M. javanica. Benincasa hispida was resistant to M. javanica and M. incognita. The compatibility between net melons and resistant rootstocks was higher than 98%.

Enxertia e sua influencia na produção de pepino e reprodução de Meloidogyne javanica e M. incognita

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Susceptibility of helminth species from horses against different chemical compounds in Brazil

By means of parasitological necropsies, the present study aimed to evaluate, in six experiments, the degree of susceptibility or resistance of different helminth species which naturally infect horses to ivermectin 0.2mg/kg, abamectin 0.2mg/kg, moxidectin 0.4mg/kg, trichlorfon 35mg/kg, ivermectin 0.2mg/kg+praziquantel 2.5mg/kg, abamectin 0.2mg/kg+praziquantel 2.5mg/kg and ivermectin 0.2mg/kg+6.6 mg/kg pyrantel. At experimental day zero, the horses were allocated to treatment groups based on average counts of strongylid eggs per gram of feces (EPG) obtained on days -3, -2 and -1. Oxyuris sp. infections were confirmed as positive or negative. All the animals in the six experiments were naturally infected by this helminth species. Each group (control or treated) consisted of six animals. All the assessed Habronema muscae populations analyzed were susceptible to ivermectin, abamectin and moxidectin. Of the six Trichostrongylus axei populations, four were susceptible to ivermectin, abamectin, moxidectin, trichlorfon and ivermectin+praziquantel, and two were resistant to abamectin+praziquantel and ivermectin+pyrantel. Both Strongyloides westeri populations analyzed were susceptible to ivermectin, abamectin, moxidectin and abamectin+praziquantel. For O. equi, resistance was found in four different populations treated with ivermectin, abamectin, moxidectin, trichlorfon and ivermectin+praziquantel. Only combinations of abamectin+praziquantel and ivermectin+pyrantel were effective against this parasite species. All the large strongyles diagnosed in the present study (Strongyus edentatus, Strongyus vulgaris and Triodontophorus serratus) were susceptible to all the chemicals tested, with the exception of trichlorfon. Of the Cyathostominae populations, one was diagnosed as resistant to ivermectin and another to trichlorfon. The remaining populations from this nematode group were considered to be sensitive to ivermectin, abamectin, moxidectin, ivermectin+praziquantel, abamectin+praziquantel and ivermectin+pyrantel. New studies should be performed in different regions to evaluate the efficacy of trichlorfon in others field populations of helminthes.

Dinâmica espaço-temporal da ocorrência de nematoides em seringueira

Pós-graduação em Ciência Florestal - FCA

Reação de araçazeiros a Meloidogyne enterolobii e enxertia da goiabeira ‘Paluma’ em portaenxertos resistentes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ocorrência de Meloidogyne graminis em grama no estado de São Paulo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo genético de populações de soja com fonte de resistência à Heterodera glycines (raça 3) oriundas de topocruzamentos tipo alimento x tipo grão

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Macronutrientes em pepino enxertado em áreas com nematoides de galhas

Macronutrients in grafted cucumber in areas with gall nematodes. Grafting is an often recommended technique for cucumber culture in areas infested with gall nematodes. This study aimed to evaluate the content of macronutrients in grafted cucumber in two rootstocks on soil infested with Meloidogyne incognita race 2 or Meloidogyne javanica, since they may decrease the effectiveness of water and nutrient absorption. One evaluated 9 treatments (factorial 3x3: nongrafted hybrid cucumber Tsuyataro, grafted on squash hybrid Shelper or grafted on squash hybrid Excitte Ikki x without inoculation, inoculation with M. incognita or M. javanica), in a randomized blocks design, with 4 replicates and 6 plants per plot. The characteristics evaluated were: macronutrient content at 72 days after transplantation and macronutrient content at the end of the cycle (99 days after transplantation). The nutrient contents evaluated didn’t differ according to the nematode species (with or without inoculation), however, there’re differences with regard to the rootstocks, with higher values of phosphorus and potassium in grafted plants and higher values of magnesium, calcium, and sulfur in nongrafted plants at the end of cycle.

Inter-relação de Meloidogyne enterolobii e Pratylenchus brachyurus em variedades de goiabeiras

Pós-graduação em Agronomia (Produção Vegetal) - FCAV