Evaluation of the protective effects of kolaviron on Kolanut-induced oxidative stress in developing rat brain

The brain is exposed throughout life to oxidative stress, and certain diseases of the brain and nervous system are thought to involve free radical processes and oxidative damage. This study is aimed at evaluating the effect of kolaviron on kolanut-induced oxidative stress in developing rat brain. Twenty-five adult pregnant Wistar rats weighing between 160 and 180g were used for the experiment. They were randomly divided into five groups of five animals each. The animals were fed with standard diets of mice cubes and water provided ad libitum. The control rats received water and cornoil, while the experimental animals received 200 mg/kg body weight of kolanut (kn), 200 mg/kg of kolaviron (kv), and 200 mg/kg body weight of vitamin E which served as a standard antioxidant with cornoil as vehicle orally in pre- and post-natal life. After birth, gross morphometry and behavioural changes of the pups of days 1, 7, 14, 21 and 28 postpartum were evaluated. Blood samples were collected from pups of day 21 for hematological, liver and renal function analyses, while the brains of pups of day 21 postpartum were preserved in phosphate buffer at a temperature of 4oC and pH 7.4 for biochemical analysis. There were significant alterations in the gross morphometry and behavioural parameters studied in the treated animals compared with the control at p< 0.05. There were elevated levels of RBC, WBC and platelets in the treated group compared with the control at p< 0.05. However, no significant change was observed in the PCV, Hb, liver and renal function parameters studied at p>0.05. A non-significant increase in levels of malondialdehyde, MDA, a bye-product of lipid peroxidation in the kolanut group was observed. However, administration of kolaviron and vitamin E non-significantly (p>0.05) reversed these changes. In conclusion, maternal consumption of kolanut induced mild oxidative stress and the administration of kolaviron and vitamin E decreased the rate at which kolanut induced oxidative stress in developing rat brain.

Synthesis, characterization, and preliminary application of new biocompatible nanogels useful as anticancer drug delivery systems

253 p.

Transformação de modelos ORM-UML

Dissertação de Mestrado, Engenharia Informática, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2014

Estudo do papel desempenhado pelo heterotetrâmero de anexina A2 em células de cancro da mama hipóxicas

Dissertação de Mestrado, Biologia Molecular e Microbiana, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2014

Liver injury from ampicillin-induced intestinal microbiota distresses in rats fed carbohydrate- and protein-rich diets

Purpose: To investigate the effect of ampicillin on rat intestinal microflora and liver in the presence of high carbohydrate and protein diets. Methods: Male Wistar albino rats were divided into four groups. The first group served as the control, the second group was treated with ampicillin (50 mg/kg for 3 weeks) and fed with a standard diet, while the third and fourth groups were treated with the same dose of ampicillin and fed with acarbohydrateand protein-rich diets, respectively, to observe the effect of diet on gut flora and liver. Fecal specimens were collected and used for qualitative determination of gut microbiota composition. Serum hepatospecific markers (AST, ALT and ALP) were estimated. The antioxidant status of liver tissues was estimated for GSH, MDA, GST, LDH and vitamin C l, in addition to sodium and potassium. Results: Administration of orogastric dose of ampicillin for 3 weeks induced inhibition of E.coli, yeasts, total anaerobes, and anaerobic lactobacilli with new growth of P. vulgaris and K. pneumonia. The levels of serum AST, ALT and ALP showed significant (p ˂ 0.05) increase to 163, 112.38 and 115.35 %, respectively in ampicillin-treated animals, compared to control. Also significant (p ˂ 0.05) increase in lipid peroxidation (120 %) and LDH (111 %) coupled with significant (p ˂ 0.05) decrease in glutathione (74.57 %), vitamin C (63.49 %) and glutathione S-transferase (41.51 %) were observed in ampicillintreated groups. No significant variation (p ˂ 0.05) in sodium and potassium levels were found between control and the treated group after 3 weeks of treatment. Conclusion: These results confirm that extended ampicillin therapy disrupts gut flora, which results in liver injury; hence, overuse of antibiotics should be avoid.

Development of paclitaxel-loaded liposomal systems with anti-her2 antibody for targeted therapy

Purpose: To develop liposome formulations containing monoclonal antibody anti-HER2 (MabHer2), and Paclitaxel (PTX). Methods: Seven different liposomal systems containing PTX, or MabHer2 or a combination of PTX and MabHer2 were made using lipid film hydration technique and sonication. The effects of liposome preparation conditions and extraction methods on antibody structure were investigated by polyacrylamide gel electrophoresis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The characteristics of the liposomes were determined by a zetasizer, while drug-loading efficiency was evaluated by high-performance liquid chromatography. The cytotoxic effect of the liposome formulations was evaluated on MDA-MB-453 (HER2+) and MCF-7 (HER2-) breast cancer cell lines by MTT assay. Results: The antibody was not significantly affected by the stress conditions and the method of extraction. The particle size of liposomes was < 200 nm while the amount of incorporated PTX was 97.6 % for liposome without cationic agent and 98.2 % for those with cationic agent. Recovery of MabHer2 was 94.38 % after extraction. Combined PTX/MabHer2 liposome was more toxic on HER2 overexpressing positive MDA-MB-453 cell line than PTX-loaded liposomes and MabHer2. MabHer2 and combined PTX/MabHer2 liposomes showed no toxic effects on HER2 overexpressing negative MCF-7 cells relative to cationic PTX-loaded liposomes. Conclusions: This results obtained show that PTX can be encapsulated successfully into liposoma systems and that owing to Her2 specific antibody, these systems can be delivered directly to the target cell.

Protective effect of rhamnopyranosyl vanilloyl isolated from Scrophularia ningpoensis Hemsl (Scrophulariaceae) root against acute liver injury in mice

Purpose: To investigate the protective effect of rhamnopyranosyl vanilloyl (RV) from Scrophularia ningpoensis root against tetrachloromethane (CCl4)-induced acute liver injury (ALI) in mice. Methods: RV was isolated from S. ningpoensis by column chromatography. ALI model of mice was established by intraperitoneal injection of CCl4. Liver index, liver function indices, as well as serum alanine transaminase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) were evaluated. Lipid peroxidation (LPO)-related indices, including malonaldehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Apoptotic proteins (Bcl-2, Bax and caspase-3) in liver tissue were determined by enzyme-linked immunosorbent assay (ELISA) and Western blot. Results: After treatment with RV (10, 20 or 40 mg/kg), liver index (5.65 - 5.21 vs. 6.68 %), ALT (90.18 - 79.68 vs. 112.47 U/L), AST (64.44 - 57.63 vs. 75.41 U/L) and TBIL (2.68 - 1.95 vs. 3.21 U/L) activities, as well as MDA (3.58 - 2.88 vs. 4.13 μmol/g), Bax and caspase-3 levels significantly (p < 0.05 or 0.01) decreased, compared with those in control group. After treatment with RV (10, 20 or 40 mg/kg), GSH (16.58 - 22.14 vs. 12.34 μmol/g), Bcl-2, SOD (86.45 - 107.61 vs. 68.43 U/mg) and GSH-Px (295.64 - 329.47 vs. 268.49 U/mg) levels or activities significantly (p < 0.05 or 0.01) increased, compared with those in control group. Conclusion: RV has protective effect against CCl4-induced ALI in mice, and the mechanisms involve the inhibition of LPO and apoptosis in liver cells. Thus, RV is a potential drug for the treatment of liver injury

Oral thearubigins do not protect against acetaminopheninduced hepatotoxicity in mice

Purpose: To investigate the potential protective effect of oral repeated doses of thearubigins against acetaminophen-induced hepatotoxicity in mice. Methods: Mice were randomly divided into six groups (n=8) and administered the following: Control group (saline), acetaminophen group (saline), N-acetylcysteine group (500 mg/kg/day), and thearubigins groups (60, 70, 100 mg/kg/day). The drugs were given orally by gavage for seven days. On day 7, 1 h after the last dose of treatment, the mice (except control group) were given a single dose of acetaminophen (n-acetyl-p-aminophenol, APAP) orally by gavage (350 mg/kg) and then sacrificed 4 h post-APAP intake. Blood was collected for biochemical measurements and their liver were subjected to biochemical and histopathological assessment. Results: The acetaminophen group showed significant increases (p < 0.001) in serum alanine aminotransferase level, hepatic cytochrome P2E1 level, and serum and hepatic malondialdehyde levels. Moreover it showed significant decrease (p < 0.001) in serum and hepatic glutathione levels. Morphologically, the liver sections showed cellular necrosis, vacuolization, and degeneration around the centrilobular veins. Pretreatment with N-acetylcysteine reversed all acetaminophen-induced changes (p < 0.001 for all biomarkers except for hepatic MDA (p = 0.014) while pretreatment with thearubigins failed to reverse any of them. Conclusion: Oral repeated doses of thearubigins failed to protect against acetaminophen-induced hepatotoxicity in mice and didn\'t affect hepatic cytochrome P2E1 level.

Serum levels of lycopene, beta-carotene, and retinol and their correlation with sperm DNA damage in normospermic and infertile men

Background: Oxidative stress in reproductive system leads to sperm DNA damage and sperm membrane lipid peroxidation and may play an important role in the pathogenesis of male infertility, especially in idiopathic cases. Antioxidants such as carotenoids function against free radical damages. Objective: The aim of this study was to determine the levels of lycopene, beta-carotene and retinol in serum and their relationship with sperm DNA damage and lipid peroxidation in infertile and normospermic males. Materials and Methods: Sixty two infertile men and 71 normospermic men participated in this study. Blood and semen samples were collected from all subjects. Sperm DNA damage was measured using TUNEL method. Carotenoids, retinol, and malonedildehyde in serum were also determined. Results: DNA fragmentation was higher in infertile group comparing to control group. Serum levels of lycopene, beta-carotene and, vitamin A in infertile men were significantly lower than normospermic men (p< 0.001, =0.005, and =0.003 respectively). While serum MDA was not significantly different between two groups, MDA in seminal plasma of infertile men was significantly higher than control group (p< 0.001). Conclusion: We concluded that lycopene, beta-carotene, and retinol can reduce sperm DNA fragmentation and lipid peroxidation through their antioxidant effect. Therefore the DNA fragmentation assay and determination of antioxidants factors such as lycopene, beta-carotene and retinol, along with sperm analysis can be useful in diagnosis and treatment of men with idiopathic infertility.

A atratividade das políticas públicas e das políticas das indústrias de tabaco: um estudo institucional

O objetivo do trabalho é interpretar as possibilidades de inserção de políticas públicas que visam à diversificação de cultivos agrícolas em municípios produtores de tabaco. Os procedimentos metodológicos empregados na condução da pesquisa caracterizam-se como um estudo descritivo, qualitativo e quantitativo. As técnicas de pesquisa empregadas foram a entrevista semiestruturada, observações e análise documental e bibliográfica. Os sujeitos da pesquisa de campo foram famílias produtoras de tabaco dos municípios de Agudo e Paraíso do Sul/RS, Brasil, selecionadas por conveniência. A análise dos dados qualitativos foi de conteúdo e a análise dos dados quantitativos ocorreu por meio da estatística descritiva, com uso do programa estatístico, com a apresentação dos dados em médias, frequências e cruzamento de médias. Como resultados, tem-se que os produtores de tabaco estão em propriedades de até 80 hectares, têm produção de alimentos para consumo próprio, porém sua principal fonte de renda é o tabaco. Na questão da intervenção pública, há dificuldade dos agricultores em reconhecer o Ministério do Desenvolvimento Agrário (MDA) como parceiro. Averiguou-se que o crédito específico para a fumicultura está sendo eliminado, apesar de não se verificar o mesmo com a produção. De toda forma, um resultado captado na pesquisa, foi de que as organizações financeiras foram consideradas parceiras do produtor, com uma frequência muito superior ao MDA. Conclui-se ser desafiadora a inserção das políticas públicas entre os produtores de tabaco destes municípios. Isso dependerá de uma ação mais efetiva do serviço público de assistência técnica e de extensão rural. A influência política das corporações de tabaco é ampla, atuam em patrocínios e projetos de responsabilidade social para transparecer uma imagem pública positiva, mas também fazem um lobby agressivo nos governos para impedir e/ou atrasar a implementação de normas restritivas de sua produção que possam comprometer seus lucros. Palavras-chave: Famílias; Tabaco; Políticas Públicas; Instituições.

Perfil gerencial de agroindústrias familiares processadoras de frutas do Acre.

O presente trabalho visou caracterizar o perfil gerencial de agroindústrias familiares do Acre. Para a caracterização pretendida foram avaliados aspectos gerenciais agrupados em cinco componentes: ?Mercado?, ?Planejamento?, ?Finanças?, ?Produção e ?Gestão de pessoas?, desdobrados em 47 variáveis. Basicamente, o estudo avaliou a presença ou ausência das variáveis escolhidas numa escala contínua de cinco níveis. A amostra foi composta por 12 agroindústrias, localizadas em oito municípios do Acre e um de Rondônia que faz fronteira com o Acre. Para a geração de dados primários, foram realizados trabalhos de campo com visitas às lideranças, seguido de entrevistas individuais com os gestores dos empreendimentos. Os escores obtidos foram analisados pela média e pelo desvio padrão. A temática agroindustrialização, principalmente sob o enfoque da pequena produção, tem sido objeto de ações governamentais e buscam, sobretudo, promover a agregação de valor aos produtos oriundos da agricultura familiar. Esse aspecto contribui para minimizar perdas da produção primária, gerar novas oportunidades de trabalho e criar condições para elevação de renda. Políticas do Governo Federal sob o protagonismo do Ministério do Desenvolvimento Agrário (MDA) têm estimulado a implantação e o fortalecimento de agroindústrias familiares em todo o país, fomentando ações estruturais, tais como: crédito para investimento e custeio, adequação à legislação, capacitação, tecnologia, promoção de negócios e acesso a mercados. Como resultado deste estudo, concluiu-se que o componente de melhor avaliação foi ?Finanças?. Já o componente ?Gestão de pessoas? obteve o menor escore entre todos. Percebe-se também que as agroindústrias estudadas carecem de uma reestruturação gerencial no sentido de ajustar-se a um padrão de gestão mais profissionalizada.

DESENVOLVIMENTO TERRITORIAL E ECONOMIA SOLIDÁRIA: CONFLUÊNCIAS E DISSONÂNCIAS NA POLÍTICA DE DESENVOLVIMENTO RURAL NO BRASIL

O presente trabalho se propõe a caracterizar as diferentes abordagens teóricas sobre os conceitos de desenvolvimento territorial e economia solidária no âmbito das ciências humanas, com vistas a identificar e analisar as concepções teóricas que fundamentam a política territorial adotada pelo Ministério do Desenvolvimento Agrário (MDA) no Brasil. Inicialmente apresentamos a trajetória das políticas territoriais do Estado brasileiro para caracterizar o atual cenário que conforma a implantação do Programa de Desenvolvimento Sustentável dos Territórios Rurais (PDSTR) instituído pelo MDA, uma vez que no plano discursivo, a adoção desse programa segue uma perspectiva antagônica as práticas governamentais historicamente caracterizadas como autoritárias e centralizadas. Posteriormente, empreendemos uma discussão conceitual sobre desenvolvimento territorial e economia solidária, visto que eles têm se constituído em referenciais centrais nos documentos oficiais que apresentam os princípios, as diretrizes e metodologias adotadas nas políticas de desenvolvimento rural brasileiras. Essa etapa corroborou na elucidação das confluências e dissonâncias existentes entre as proposições conceituais da política territorial do MDA e as concepções teóricas defendidas no plano acadêmico. Este estudo se sustenta numa ampla pesquisa documental, realizada através de consultas aos documentos eletrônicos referenciais da atual política territorial, revistas, mapeamentos e relatórios de avaliação do processo de implantação da política territorial em todo o país.

A TERRITORIALIZAÇÃO DA AGROECOLOGIA NO TERRITÓRIO RURAL DO VALE DO RIBEIRA, PARANÁ, BRASIL

O trabalho visa o entendimento sobre o processo de territorialização da agroecologia como elemento da política de desenvolvimento rural sustentável, no Território Rural do Vale do Ribeira, Estado do Paraná, Brasil. A partir de 2005, ocorre a implementação desse Território, proposta pelo Ministério do Desenvolvimento Agrário (MDA). Um dos objetivos desse programa é reduzir as desigualdades econômicas e sociais, atuando preferencialmente em espaços de elevada concentração de agricultores familiares, assentados da reforma agrária e acampados. Nesse sentido, torna-se importante analisar o processo de desenvolvimento da Agroecologia, através de entrevistas realizadas com os agricultores e a análise da proposta do MDA. A agroecologia é considerada um elemento importante de inovação em áreas rurais, colaborando no processo de desenvolvimento rural de forma mais sustentável. O desenvolvimento territorial como uma nova concepção de desenvolvimento requer a discussão dos conceitos de desenvolvimento, território e desenvolvimento territorial, o que vem auxiliar a entender o processo de territorialização da agroecologia no Território Rural do Vale do Ribeira.

Effectivity of Various β-Carotene Concentration on Quality of Frozen-Thawed Semen of Garut Rams

The purpose of this research was to evaluate the quality of frozen-thawed semen of Garut rams that cryopreserved with Tris extender containing the various β-carotene concentrations. Semen was collected from four mature Garut rams using artificial vagina once a week. Immediately after initial evaluation, semen was divided into four parts and diluted with Tris extender containing 5% glycerol + 0% (control), 0.001% (Kt0.001), 0.002% (Kt0.002), and 0.003% (Kt0.003) β-carotene, respectively. Semen was loaded in 0.25 ml mini straw with the concentration of 200 million motile sperm. Semen was equilibrated at 5ºC for three hours, then frozen and stored in liquid nitrogen container for 7 days. Quality of processed-semen including motility, live sperm, intact acrosomal cap (IAC), and intact plasma membrane (IPM) were evaluated after diluted, equilibrated, and thawed, respectively. Concentration of malondialdehide (MDA) semen after thawing were evaluated. Data were analyzed as completely randomized design with four treatments and nine replicates. Means values were compared by least significant difference test at 0.05 significant level. Results indicated that mean value of post thawing motility and live sperm for Kt0.002 (50.55% and 56.78%) were significantly higher (P<0.05) than Kt0.001 (46.11% and 52.89%), Kt0.003 (46.67% and 53.33%) and control (46.67% and 52.33%). Mean value of post thawing IAC and IPM for Kt0.002 (51.00% and 53.78%) were significantly higher (P<0.05) than control ( 47.11% and 48.44%), but not significantly different with Kt0.001 (49.00% and 50.00%), and Kt0.003 (48.89% and 49.67%). MDA concentration of frozen-thawed semen for Kt0.001 (3.37 mg/kg), Kt0.002 (3.80 mg/kg), and Kt0.003 (4.61 mg/kg) were significantly lower (P<0.05) than control (5.24 mg/kg). in conclusion, concentration of 0.002% β-carotene in Tris extender is the optimal dose in improving frozen semen quality of garut rams. (Animal Production 7(1): 6-13 (2005) Key Words : β-carotene, frozen-thawed semen, intact plasma membrane, MDA, Garut Rams