Influência da adição de fator de crescimento fibroblástico 10 (FGF10), durante a maturação oocitária, na produção in vitro de embriões bovinos

The Brazilian livestock stands out for having the world largest commercial herd of cattle and leads meat exportation and production of bovine embryos. The in vitro production (IVP) of embryos is considered an effective option to overcome problems such as infertility in cows with high economic value and also for genetic improvement of cattle. The in vitro oocyte maturation is an essential step to the success of IVP, but is still considered poor when compared to in vivo maturation. Recent studies have suggeested an important role of Fibroblast Growth Factor 10 (FGF10) on the in vitro maturation of oocytes, which favored the expression of genes related to oocyte maturation and cumulus cell expansion. Aware that maturity stage influences the final production of blastocysts, we aimed study to verify if the addition of FGF10 into the maturation medium is able to affect positively the IVP of bovine embryos. Hence, FGF10 was added to maturation in five different concentrations: 0.5 ng/mL (group 0.5), 2.5 ng/mL (group 2.5), 5 ng/mL (group 5), 10 ng/mL (group 10) and 50 ng/mL (group 50). Additionally, two other maturation groups were used, group BSA (Bovine Serum Albumin, 4 mg/mL) and group FCS (Fetal Calf Serum, 10%). The rates of cleavage, morula and blastocyst were analyzed by Analysis of Variance (ANOVA), differences of P<0.05 were considered significant. Cleavage rates did not differ between the seven groups. On the other hand, morula rate on FCS group was higher than groups BSA, 0.5, 10 and 50 (P<0.05), but did not differ among groups treated with intermediate doses of FGF10 (2.5 and 5). FCS group presented higher blastocyst rate compared to all other groups that were well below the FCS group (P<0.0001). Therefore, the use of FGF10 during oocyte maturation did not affect positively embryo development on the IVP of bovine embryos

Diagnóstico antropogeomorfológico em bacia hidrográfica urbana: o caso do Córrego do Jardim São João, Araras (SP)

Man can transform the nature according to their needs through technique which enables agriculture, cattle raising, constructions of buildings for housing, transport routes, etc. This action has brought significant transformations to the environment, and relief is one of the most changed elements, mainly at urban areas, which concentrates most of the Brazilian population. In these areas geomorphologic systems have undergone alterations in their flow of matter and energy by the action of man. Thus, it is possible o understand the action of man as geomorphologic action, nowadays, the tecnógeno, since the human action can interfere qualitatively and quantitatively in the input and output of these systems. The anthropic geomorphology has contributed to the analyses of the effects due to the urbanization at the form and at the geomorphologic process in the urban watersheds, indicating the transformations at the shape of the relief and the alterations due to the acceleration of the flow of matter and energy. From these questions, this study had the main diagnose the anthropogenic changes of the relief at the basin of Corrego do Jardim São João, Araras (SP), comparing two scenes, 1997 and 2006, through geomorphological mapping evolutionary in the area. The geomorphological feature altered at the basin are directly linked to the use of soil, mainly at the urban use, which caused effects in the relief at the studied area, making possible to define periods of the urban evolution and impacts caused by the urbanization. Among the changes it was found the decrease of the topographic breaks and at the riverbed channels, as the increase of the accumulation of the plain and riverbed terrace. The elaboration of a letter of the legal restrictions of the basin make possible to evaluate some of the environmental urban problems that occur, which are due to the occupation of the relief in the areas of permanent preservation that brings significant impacts to...

Caracterização da comunidade de mamíferos de médio e grande porte na Fazenda Nova América, Cabrália Paulista – SP

Após a expansão das fronteiras agrícola e pecuária e com o atual crescimento da indústria de papel e celulose, mosaicos compostos por remanescentes de floresta secundária intercalados com áreas de cultivo de eucalipto se tornaram uma paisagem freqüente no Estado de São Paulo. A queda na biodiversidade em qualquer monocultura é notável quando comparada aos fragmentos de florestas naturais, entretanto estudos sobre a dinâmica das espécies inseridas nesta nova condição ainda são escassos. O objetivo deste estudo é caracterizar a comunidade de mamíferos de médio e grande porte na Fazenda Nova América, localizada no município de Cabrália Paulista-SP, de forma a subsidiar ações voltadas ao manejo de habitat e conservação. O principal método de coleta foi o registro de pegadas em transectos lineares, para o estabelecimento da riqueza, abundância e estimativa da diversidade. Para isso, foram realizadas 4 campanhas de campo onde eram percorridos 5 trajetos de 2 km e largura variável, distando, no mínimo, 500 metros entre si. Foram realizadas três repetições em cada trajeto. Como metodologia complementar foram utilizadas parcelas de areia, observação direta de animais, de outros vestígios, entrevistas e captura fotográfica. Foram obtidos registros de 24 espécies de mamíferos, distribuídos em 12 famílias e 6 ordens. A observação de rastros em transectos lineares se mostrou eficiente, amostrando cerca de 80% das espécies presentes na área. A espécie mais encontrada foi o cachorro-domato (Cerdocyon thous), presente em todos os ambientes. As espécies com maior abundância na fazenda foram as generalistas. As áreas de Eucalipto apresentaram uma riqueza e uma abundância de espécies menor que os fragmentos de mata, visto que a monocultura acaba limitando os recursos disponíveis. Mas quando o cultivo ocorre de forma sustentável, pode-se formar um novo ambiente para a fauna e flora locais

Anormalidades fetais e placentárias decorrentes da produção in vitro de embriões bovinos

In vitro production of bovine embryo (IVP) has become a remarkable assisted reproduction biotechnology in Brazil, once it is considered an important tool for the genetic improvement of the herd. However, many abnormalities are associated with IVP technologies, such as higher incidences of embryo loss, abortions, hydrallantois and dystocia, prolonged gestation, increased birth weight and high perinatal mortality. Collectively, these abnormalities are known as “Large Offspring Syndrome”, which has limited the large-scale use of IVP technologies in cattle industry

Crise da cafeicultura e seus efeitos socioeconômicos no município de Dracena (SP)

The coffee crisis in the 1980s reached deeply municipality of Dracena, considering that the coffee had significant social and economic importance to the area. In this context, the research aimed to analyze the socioeconomic consequences that this crisis has provided small rural proprietors of the county. To achieve this goal we seek to articulate the theoretical reference, with data from secondary sources, interviews with the main responsible for the agricultural sector and an application questionnaire to 21 small rural proprietors of the county. It was found that, due to the dependence relationship with the coffee, the economy of the city suffered from the attack of said culture during the 1980s. Rural producers who cultivated such crops as the main, had to readjust productively to remain in the countryside, but the economic and social development that coffee was brought in earlier times was not rescued. As a consequence, there has been an intensification of evasion demographic of rural municipality, a process that had begun in the 1970s and was strongly reinforced by the crisis of the coffee culture. We note that from the 2000s, the situation became better for small rural proprietors in order that public policies implemented now serve, in part, the needs of those with greater efficiency. This situation can be evidenced by a slight reduction in the number of rural people dracenense. Despite improvements recorded since the 2000s, the situation is still not ideal, given that many small proprietors surveyed expressed dissatisfaction. In this context, we note that the performance and joint of federal, state and municipal government are essential so that public policies are efficient, thus providing decent conditions for social reproduction of small rural proprietors and their dependents in the countryside

Zoneamento geoambiental do município e região de Franca-SP

O município de Franca-SP situa-se na Província Geomorfológica das Cuestas Basálticas no nordeste paulista, a qual possui características ambientais próprias. De uma forma geral, a região de Franca enfrenta problemas ambientais como a suscetibilidade à erosão linear e laminar, aos movimentos de massa e ao assoreamento dos canais de drenagem. Estes problemas, por sua vez, induzem a situações socioeconômicas, como o incremento da pobreza, aglomeração urbana e, uma emergente demanda da sociedade por mudanças no âmbito da gestão local. Assim, devem ser efetuados estudos detalhados sobre o meio, a fim de minimizar e/ou evitar tais problemas. Desta forma, propos-se a realização do Zoneamento Geoambiental, o que implica no levantamento, análise, interpretação e correlação dos diferentes componentes ambientais, visando dar subsídio a órgãos públicos e proprietários rurais, tanto na fase de planejamento como na implantação de culturas agrícolas, pecuária, reflorestamento e uso da água superficial (lençol freático), de maneira racional e ambientalmente sustentável, garantindo assim a ocupação adequada do solo e a melhor conservação dos recursos naturais. O trabalho iniciou-se com a organização e reinterpretação dos dados pré-existentes (topográficos, geológicos, pedológicos, geomorfológicos, morfoestruturais, de coberturas de alteração intempérica e sócio-econômicos) e geração e/ou reorganização de novos dados e propostas para a minimização dos impactos sócio-ambientais (rede de drenagem, hipsométricos, morfométricos, tectônicos, geológicos, geomorfológicos, climáticos e bióticos) com a utilização de técnicas de fotointerpretação de diversos sensores remotos e de campo. Deste modo, a correlação de todas as informações geoambientais relativas ao município proporcionou a delimitação das suas zonas e subzonas geoambientais... (Resumo completo, clicar acesso eletrônico abaixo)

Tratamento orgânico de resíduos de rúmen bovino

The organic wastes need to be adequately managed, in order to avoid the environmental pollution and damage to the public health. So, this work aimed to study the composting process using two methods: manual and mechanized, for the treatment of bovine ruminal waste. This residue is generated in large proportions during the bovine slaughter process, and it can lead environmental degradation and contamination, or even damage to the public health, when not treated. For the initial adjustment of the composting parameters, it was incorporated the residue of coffee husks. The manual composting system was done by the manually aerated piles, while the mechanized composting system was done in a reactor coupled to a compressor that enabled the aeration of the system. The proportions used in both systems were: 90% bovine rumen (R) and 10% coffe husks (CC); 85% bovine rumen and 15% coffe husks; 80% bovine rumen and 20% coffe husks. The parameters determined during the monitoring of the composting process were: temperature, pH, moisture, organic matter, ash, organic carbon, Kjeldahl nitrogen and C/N ratio. The results obtained during the monitoring of the piles and reactors presented similar behavior, except for the parameters Kjeldahl nitrogen and C/N ratio. When compared to the “Instrução Normativa no 25 de 23/07/2009 do Ministério da Agricultura, Pecuária e Abastecimento”, the organic produced composts with the best results were: pile 2 (85% R; 15% CC) and reactors 1 (90% R; 10% CC) e 2 (85% R; 15% CC)

Produção e aplicação de adubo orgânico, proveniente da compostagem de resíduos da FCT/UNESP

This work presents the objective of producing organic fertilizer from the waste generated at FCT/UNESP and its use in the growing sunflower. For this, there were collected wastes of pruning/weeding and organic waste from the canteen. It were made two types of treatments, one conventional and the other mechanized, using three mass ratios: ratio 1 (30% wastes of pruning/weeding (RP) + 70% organic waste (RC)), ratio 2 (50% RP + 50% RC) and ratio 3 (70% RP + 30% RC). The conventional system was done windrow with turning manual and the mechanized system was developed in reactors by injecting compressed air. The compounds produced were analyzed in terms of pH, organic matter, organic carbon, mineral residue, nitrogen, phosphorus and potassium, which could be compared to the values stipulated by the Instrução Normativa n° 25 de 23/07/2009 do Ministério da Agricultura, Pecuária e Abastecimento. The compounds produced were used on growing sunflowers in 14 different conditions for the systems manual and mechanized, totaling 26 treatments with the following proportions... (Complete abstract click electronic access below)

Explorando as consequências das preferências alimentares dos brasileiros sobre o uso da terra e emissões de CO2

O consumo de carne bovina pela população brasileira está relacionado a aspectos que sejam sociais ou de saúde. Além disso, pode afetar também o nosso meio ambiente, aumentando impactos ambientais. Em se tratando do Brasil, temos uma grande demanda deste alimento, influenciada pelos hábitos alimentares da população, exigindo, por conseguinte, uma grande produção. Através da construção de cenários e modelagem ambiental, este trabalho avaliou algumas das consequências ambientais da pecuária de corte no Brasil. Para tanto, usamos estimativas de consumo da população, produção da carne bovina e também de valores que relacionem o uso da terra com as emissões de gás carbônico para avaliar os problemas ambientais. Contabilizamos as emissões de gases de efeito estufa associadas as mudanças de uso da terra, fermentação entérica e o manejo de dejetos do gado bovino. Obtive que em alguns cenários extremistas como o Con2a as emissões foram no total de 1,77 PgCO2e anualmente. Enquanto no cenário nacional obtive um total de 1,2 PgCO2e das emissões anuais associadas a todos os seus setores. Em nossos resultados notamos a forte influência do nível de consumo de carne e do sistema de manejo nas emissões de gases efeito estufa dos cenários estudados

Mapeamento geoestatístico da vulnerabilidade do Aquífero Guarani no município de Ribeirão Bonito - SP

The Guaraní aquifer has relevant importance both as a source of water for several urban centres and the development of agriculture and livestock. In recharge areas the aquifer is free and, therefore, subject to contamination of effluents and tailings deposited on soils that cover it. Thus, it becomes crucial not only its protection at all levels, as the knowledge of its degree of natural vulnerability. The present work used geostatistics modeling techniques to study the natural vulnerability of the Guaraní aquifer in the city of Rio Bonito, State of São Paulo, southeastern Brazil, where the Guarani aquifer is exposed. These techniques, extensively used in evaluation studies of mineral deposits and oil tanks, can be adapted to produce a spatial classification or a regionalisation of probabilistic indices of vulnerability. By ordinary kriging method maps of vulnerability classification were obtained. To determine the vulnerability of the aquifer was employed the Aquifer Vulnerability Index (AVI), which requires knowledge of unsaturated zone thickness and permeability. The final product was a map with probabilistic index of vulnerability of the Guaraní aquifer, which presented values between 0 to 0.33 years, framing the area studied in AVI class extremely high vulnerability

Dinâmica folicular ovariana e bases para a aplicação das biotécnicas da reprodução em bovinos

In the last years Brazil has achieved a leading position in worldwide cattle breeding. This country is consolidating the practice of an intensive and technical level cattle breeding, due to greater emphasis on reproductive efficiency and genetic improvement. The attention given to reproductive efficiency became to enhance the genetic improvement process, by exploiting the full reproductive potential of the animals. The biotechniques applied to reproduction arised to expand the reproductive potential for greater economic return. Are the most important reproductive biotechniques the FTAI and in vivo and in vitro embryo production for embryo transfer. Different hormonal treatments are consolidating to interfere with normal physiology in order to achieve the desired goals. The FTAI works to increase the reproductive rates of cattle through pre-booked artificial insemination in all the females in reproduction, without the need for estrous detection. The in vivo and in vitro embryo production aims to distribute the genetics of males and females genetically improved by transferring their embryos to genetically inferior females. Superovulation treatments designed to prevent the mechanism of dominance, which results in many follicles ovulating simultaneously for in vivo embryo production. To the in vitro embryo production, the technique of ovarian puncture guided by transvaginal ultrasound allows to aspirate a number of quality oocytes. The application of biotech is based on knowledge of the female and ovarian reproductive physiology and knowledge of their acting in different breeds and environments.

As transformações do espaço geográfico e sua relação com os processos erosivos de uma área do noroeste de Regente Feijó/SP

O presente trabalho discute a relação sociedade e natureza a partir do estudo de caso dos processos erosivos identificados em um recorte espacial, localizado no Noroeste do município de Regente Feijó/SP. As dinâmicas socioeconômicas que se processaram no Oeste Paulista e, consequentemente, no município de Regente Feijó, ao longo das décadas de ocupação, são evidências das lógicas de apropriação ancoradas em pressupostos de exploração massiva dos recursos naturais, tendo em vista a produção de gêneros agrícolas valorizados no mercado internacional, tais como o café, o algodão e a cana-de-açúcar. Além disso, a pecuária também se constituiu como importante base econômica regional e, somada aos demais usos agrícolas, contribuiu para o depauperamento dos solos, o assoreamento dos cursos d’água e o surgimento de erosões (laminares e lineares) nas áreas rurais e urbanas. Neste sentido, além da discussão envolvendo os problemas desencadeados pelo agravamento dos processos erosivos aos pequenos produtores rurais e aos moradores do Distrito de Espigão, é evidenciada a importância das práticas conservacionistas como forma de contingenciamento de tais fenômenos

Análise de patologias de estradas vicinais para proposta de intervenção mínima visando a sua perenização

The unpaved back roads, despite their simplicity, are of paramount importance to the municipalities that their economy basis are in agri-livestock and a large portion of their population lives in rural areas, as it is from these pathways that flow of production is performed and ensuring access of the rural population to urban centers is allowed. Aiming the importance of these rural roads, this work studies the case of pathologies found in local roads of São Luiz do Paraitinga, located in the Vale do Paraíba upstate São Paulo, due to lack of a periodic maintenance program the roads not withstood the heavy rains late in the year 2009 and early 2010, generating major impacts on society and the economy of the city. From surveys conducted in the field, it was analyzed the various pathologies found (pathology drainage, slope and platform) checking also the nature of their occurrence, therefore propose solutions for intervention in rural roads, to decrease the susceptibility of these roads during the rainy season

Aplicação de técnicas alternativas para recuperação de foco eosivo em área rural situada no município de Álvares Machado – SP Presidente Prudente 2012

When handling soil conservation practices isn’t applied correctly, they cause problems at the level of structure, cohesion and fertility in soils and lack of water availability. In the case of this monograph, conducted in a rural property located at Álvares Machado City, identified that most areas are intended for use activities of beef cattle and dairy and subsistence agriculture, which historically addition to withdraw native flora, resulting in problems such as compaction and increase soil exposure to climatic agents, generating a serious frame of erosion problems. In the case of water from rainfall, the soil exposed, causes the “splash” effect and therefore when it becomes runoff carries the spalled particles mainly for low land areas. Without the vegetation, especially trees or shrubs species, the velocity of runoff increases, initiating the appearance of furrows, which consequently may become a kind of ravine erosion and gully, which makes invalid any area in your surroundings... (Complete abstract click electronic access below)

Alterações hepáticas em cães intoxicados experimentalmente por sementes de Crotalaria spectabilis

Pós-graduação em Medicina Veterinária - FCAV