Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)

Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the “type” locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA<sub>3sub> fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by CMA<sub>3sub>, were located distally on the long arm of pair 7 (7q). DAPI<sub>+sub> heterochromatin was predominantly centromeric, with some small pericentromeric bands. Although the C-banding patterns of other Bolitoglossa species are so far unknown, cytogenetic studies conducted in other Plethodontid salamanders have demonstrated that pericentromeric heterochromatin is a useful cytological marker for identifying interspecific homeologies. Species diversification is usually accompanied by chromosomal changes. Therefore, the cytogenetic characterization of Bolitoglossa populations from the middle and western Brazilian Amazon Basin could identify differences which may lead to the identification of new species.

Variabilidade das sub-populações de espermatozóides avaliados pela cinética em sistema computadorizado e combinação de sondas fluorescentes como praâmetro quantitativo do sêmen congelado de ovinos

Pós-graduação em Medicina Veterinária - FMVZ

Impactos Ambientais da Mineração de Argila para Cerâmica Vermelha Na Sub-bacia do Ribeirão Jacutinga – Rio Claro e Corumbataí (SP)

O presente estudo teve como objetivo caracterizar os impactos decorridos da mineração de argila para cerâmica vermelha por 10 produtores localizados na Sub-bacia do Ribeirão Jacutinga. A extração da matéria-prima proveniente do manto de alteração da Formação Corumbataí constitui uma forma de mineração de pequeno porte, a qual acumula impactos por um longo período. Para caracterização dos impactos fez-se uso de Sistema de Informação Geográfica para análise de imagens orbitais (TM-Landsat) e dados obtidos em base 1:10.000 e observação in situ. Os principais impactos detectados foram: abertura de cavas em locais de várzea, formação de lagos em cavas abandonadas, modificação da paisagem, falta de ações de recuperação de áreas lavradas e corte de vegetação nativa. Foram identificadas as áreas de maior fragilidade ambiental na sub-bacia, as quais estão associadas a zonas de maior declividade ou a locais de extração. A exploração mineral trouxe significativas alterações no quadro natural desta sub-bacia e o conhecimento sobre as limitações ambientais, aptidões para exploração e formas de mitigação dos impactos, mostram-se de extrema necessidade para uma ocupação mais racional do espaço, respeitando as limitações impostas pelo meio físico e prezando por ações sustentáveis.

Mapeamento do Uso do Solo Nas Sub-bacias dos Córregos São José e Água do Ventura, Bauru (SP)

O presente trabalho teve como objetivo, mapear o uso da terra das sub-bacias dos córregos São José e Água do Ventura no ano 2000, e verificar, como esta ocupação vem atuando nos processos de assoreamento dos corpos de água. O estudo teve como base, um mosaico em escala nominal aproximada de 1:10.000, produzido a partir de fotografias aéreas verticais do ano 2000, em escala nominal aproximada 1:30.000. Os resultados permitiram constatar que a pastagem foi a maior ocupação do solo, com 430,89 ha, correspondente a 65,21% da área estudada. Devido ao manejo inadequado do solo, é a cobertura vegetal que mais produz sedimentos por ocasião das chuvas, merecendo cuidados especiais para evitar os processos erosivos do solo. Foi constatado também, que os serviços de adequação e melhoria de estradas rurais, executados nas estradas municipais BRU-030 (antiga Bauru/Piratininga) e variante da BRU-030, atingiram seus objetivos, pois, praticamente, acabaram com a erosão das estradas, permitindo tráfego o ano inteiro, reduzindo o custo de manutenção das estradas e aumentando o abastecimento do lençol freático, além de outros benefícios.

Industrialization in Sub-Saharan Africa and import substitution policy

This article aims to contribute to the understanding of the process of import substitution in Sub-Saharan Africa. The process of industrialization in Sub-Saharan Africa occurred in two phases: a first step, even very early during the colonial regime began around the 1920s and ended in the late forties; a second phase of industrialization began in the late fifties and gained momentum in the sixties, when import substitution was implemented more widely. Although these countries were the last to embark on the strategy of import substitution, they followed the same steps of Latin American countries, and as the structural domestic and external constraints were too strong, the failure of the policy of import substitution arrived early and the negative impact on these economies had a greater magnitude.

Germinação em temperatura sub-ótima de embriões de Inga vera subsp. affinis obtidos sob diferentes condições ambientais

Physiological responses like the thermal requirements for germination may be influenced during seed formation by environmental conditions such as water availability and temperature. Here we analyzed germination of embryos of Ingavera subsp. affinis from different sources in response to various temperature regimes, including calculation of degree days and accumulated rainfall during development and maturation. Embryos were taken from ripe fruits collected on trees located in São Paulo and Minas Gerais, and were analyzed as to germination, water content and dry matter content. The results showed that the origin of the embryos was related to variation in response under sub-optimal temperatures. Depending on the origin, I.vera subsp. affinis embryos were able to germinate even at low temperatures (10 to 15 ºC), with better performance attained from 20 to 25 ºC. This variation may be associated, among other factors, to environmental conditions during maturation and to the maturation stage at seed dispersal.

Balanço hídrico na sub-bacia hidrográfica do Boi Branco – SP para gestão sustentável da agricultura irrigada

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Modelagem dinâmica temporal para a avaliação da disponibilidade hídrica e seus efeitos na sustentabilidade do sistema da sub-bacia do córrego do Boi Branco - SP

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Characterization of monocarboxylate transporters (MCTs) expression in soft tissue sarcomas: distinct prognostic impact of MCT1 sub-cellular localization

Background: Soft tissue sarcomas (STSs) are a group of neoplasms, which, despite current therapeutic advances, still confer a poor outcome to half of the patients. As other solid tumors, STSs exhibit high glucose consumption rates, associated with worse prognosis and therapeutic response. As highly glycolytic tumors, we hypothesized that sarcomas should present an increased expression of lactate transporters (MCTs).Methods: Immunohistochemical expression of MCT1, MCT2, MCT4 and CD147 was assessed in a series of 86 STSs and the expression profiles were associated with patients' clinical-pathological parameters.Results: MCT1, MCT4 and CD147 were mainly observed in the plasma membrane of cancer cells (around 60% for MCTs and 40% for CD147), while MCT2 was conspicuously found in the cytoplasm (94.2%). Importantly, we observed MCT1 nuclear expression (32.6%). MCT1 and MCT4, alone or co-expressed with CD147 in the plasma membrane, were associated with poor prognostic variables including high tumor grade, disease progression and shorter overall survival. Conversely, we found MCT1 nuclear expression to be associated with low grade tumors and longer overall survival.Conclusions: The present work represents the first report of MCTs characterization in STSs. We showed the original finding of MCT1 expression in the nucleus. Importantly, opposite biological roles should be behind the dual sub-cellular localization of MCT1, as plasma membrane expression of MCT1 is associated with worse patients' prognosis, while nuclear expression is associated with better prognosis.

Double-lumen catheter in the right jugular vein induces two sub-endothelial abscesses in an unusual place, the transition between the superior vena cava and the right atrium: a case report

Endocarditis is a type of infection that is common in internal medicine wards and in haemodialysis clinics. The location that is most affected are the heart valves. Herein, we report a case of an uncommon abscess, a sub-endothelial abscess between the transition of the superior vena cava and the right atrium. There were several emboli to the lung and foot, and the agent was related to Staphylococcus aureus and a double-lumen catheter. Usually, this type of abscess is located in valves, either the tricuspid valve if related to catheters or injection drug use or the mitral valve if related to other causes. An exhaustive review was made, but we found no information about the location of this abscess and the rarity of the event motivating the report of infection.

Avaliação espaço-temporal da distribuição de fósforo na água e nos sedimentos de fundo das sub-bacias dos rios Cuiabá e São Lourenço e em algumas baias do Pantanal Matogrossense

Pós-graduação em Química - IQ

Avaliação da resposta imune sistêmica e compartimentalizada em pacientes portadoras de cãncer de ovário: sub-titulo / (se houver)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O caminho novo: o Vale Histórico da Serra da Bocaina - opulência e decadência da sub-região paraibana paulista (reintegração de um espaço geográfico 'deprimido')

A pesquisa resgata o trecho paulista do “Caminho Novo”, antiga estrada geral de São Paulo, ligação terrestre com o Rio de Janeiro, a partir do século XVIII. No chamado Vale Histórico da Serra da Bocaina, cujo relevo se apresenta muito movimentado, a cultura do café penetrou em território paulista. Esta sub-região do Vale do Paraíba tornou-se uma das mais prósperas do país. A fase posterior, de queda na produção cafeeira, transformou-a numa região “deprimida” e estagnada. Atualmente, existem esforços para, através dos diversos ramos do turismo, reativar e dinamizar esta região vale-paraibana paulista, de modo a reintegrá-la ao pujante sistema econômico de São Paulo.

Caracterização hidroquímica dos sistemas aquíferos Serra Geral e Guarani da sub-bacia do Rio Pardo e municípios adjacentes, no sudoeste do estado de São Paulo

The main purpose of this work is to evaluate the chemical composition of the groundwaters from Guarani Aquifer System and Serra Geral Aquifer System in the Rio Pardo basin, located at the southwestern of São Paulo state. To the hydrochemical characterization, physicalchemical analyses from 32 sampled wells were used. These results were plotted in Piper diagrams allowing the classification of groundwater as calcium or calcium-magnesium bicarbonate type and sodium bicarbonate type for both aquifer systems. Distribution of hydrochemical facies over the study area was obtained using Stiff diagrams. The groundwater can be classified, representing in this sequence its hydrochemical evolution. The recognized mechanisms responsible for groundwater evolution are dissolution of minerals magnesium, such as olivine, present in the basalt, dissolution of feldspars and removal of the carbonate cement of the sandstones mineral framework. Ionic exchange may represent an important processes in the groundwater evolution, responsible for the increase in the sodium concentration and decrease of calcium

Efeito da prática do futebol no desempenho em agilidade, velocidade e coordenação de meninos na categoria dentinho ou sub 11 (10 e 11 anos de idade)

In Brazil, the practice of Soccer is encouraged very early. It is common remark that parents enroll their sons into soccer schools, promoting the involvement of these children in formal practice. However, there are not many studies that assess the effects of this practice in the physical capacities of children. Thus, the question that guides this study is: children who practice soccer systematically present better development of physical, especially agility, coordination and speed? The objective of this study was to compare the performance in velocity tests, agility and coordination of boys of 10 and 11 years, category dentinho or Sub-11, practitioners of soccer in schools and non practicing. The study included male children, born in 1997 and 1998, formed two groups: group of practitioners of soccer and group of non practicing. To belong to the group of practitioners, the child was regularly engaged for at least 1 year, with 1 hour daily and 2 to 3 times per week. The participants of the group of non practicing were not involved in regular practice of soccer and were paired in weight and stature with participants of the group of practitioners of soccer. Each participant was individually assessed in neutral environment and without foreign interference. For the assessment of the agility, was utilized the (shuttle run) test. The race test 30 meters stopped was employed for the assessment of the speed. The test of fiddling between the cones was employed to measure the coordination. The time spent to perform 1 attempt to each test was used for the comparison between groups. These data were treated, initially, by descriptive statistics (mean and standard deviation) and, subsequently, were compared by means of Anova, for independent samples. The significance level was maintained at 5%. Through analysis of the results the conclusion is that the regular practice ...(Complete abstract click electronic access below)