An overview of the higher level classification of Pucciniomycotina based on combined analyses of nuclear large and small subunit rDNA sequences

Mycologia, Vol. 98, nº6

Análise da capacidade vital e da máxima capacidade de insuflação: relação com a função bulbar nos doentes com Esclerose Lateral Amiotrófica

Introdução: A Esclerose Lateral Amiotrófica (ELA) é considerada uma das doenças neuromusculares (DNM) com características mais limitantes e fatais, sendo caracterizada por fraqueza muscular progressiva. Objectivo: Analisar a evolução da Capacidade Vital e da Máxima Capacidade Inspiratória em doentes com ELA e a sua relação causal com a função bulbar. Procedimentos: A partir de uma população alvo de 203 pacientes com ELA, foram incluídos no estudo aqueles que tinham entre 2 a 4 testes de função respiratória considerados válidos (CV <2000ml) perfazendo um total de 22 indivíduos. As medidas CV e MCI foram analisadas. Resultados: A CV diminuiu ao longo do tempo (media±desvio padrão no 1ºmomento de avaliação =1779,5±692,3; media±desvio padrão no 4ºmomento de avaliação =1108,6±475,7). O comportamento da MCI foi mais estável ao longo dos 4 momentos de avaliação. Avaliou-se a correlação entre as duas variáveis, destacando-se a relação existente entre as duas nos doentes bulbares (coeficiente = 1). Quando avaliada a diferença entre a CV e a MCI, verificamos que o nível de significância no grupo (n=22) aumentou ao longo do tempo. Ao comparamos esta diferença por subgrupos, registou-se uma diferença significativa apenas nos doentes bulbares (1ºmomento – p=0,008 e último momento de avaliação – p 0). Conclusão: Nos doentes com disfunção bulbar a CV diminui ao longo do tempo. A relação entre MCI e CV é um bom factor preditivo da evolução e prognóstico da doença e de diagnóstico do envolvimento da musculatura bulbar.

ALSMon: sistema para monitorizar ALS

Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Informática e de Computadores

Seroepidemiology of hepatitis a and b in two urban communities of Rio de Janeiro, Brazil

Hepatitis B markers were determined in 397 individuals from Niterói and 680 from Nova Iguaçu and prevalences of 9.1% (1.0% of HBsAg and 8.1% of anti HBs) and 11.1% (1.8% of HBsAg and 9.3% of antiHBs) were found, respectively. The comparative prevalence of both markers in relation to age showed a higher prevalence of HBsAg in the group 21-50 years old. Considering the antiHBs antibody, it was demostrated a gradual increase with age, reaching 14.9% in Niterói and 29.1% in Nova Iguaçu in individuals over 51 years old. For hepatitis A, in 259 samples from Niterói, equally distributed by age groups, an overall prevalence of 74.5% of anti-HAV antibodies was found. This prevalence increases gradually reaching 90.0% at age over thirty. In 254 samples from Nova Iguaçu analysed, a prevalence of 90.5% of antibodies was encountered when the same criteria of distribution of samples were used. This level of prevalence reached 90.0% already in the age over ten years old. The tests were performed by enzyme immunoassay with reagents prepared in our laboratory.

Sistema de gestão de transporte de envios parcelares

No ambiente empresarial actual, cada vez mais competitivo e exigente, é um factor fundamental para o sucesso das empresas a sua capacidade de atingir e melhorar os níveis de satisfação exigidos pelos clientes. Para identificar as melhorias a implementar, as empresas devem ser capazes de monitorizar e controlar todas as suas actividades e processos. O acompanhamento realizado às actividades delegadas a empresas externas, como por exemplo o transporte de mercadorias, é dificultado quando os prestadores destes serviços não possuem ferramentas de apoio que disponibilizem informação necessária para o efeito. A necessidade de colmatar esta dificuldade na recolha da informação durante a distribuição de uma encomenda na empresa Caetano Parts, uma empresa de revenda de peças de substituição automóvel, levou ao desenvolvimento de uma ferramenta que permite fazer o seguimento de uma encomenda em todas as suas fases, permitindo ao responsável pelas operações acompanhar o estado da encomenda desde o instante em que a encomenda é colocada, passando pelo seu processamento dentro das instalações, até à sua entrega ao cliente. O sistema desenvolvido é composto por dois componentes, o front-end e o back-end. O front-end é composto por uma aplicação web, e por uma aplicação Android para dispositivos móveis. A aplicação web disponibiliza a gestão da base de dados, o acompanhamento do estado da encomenda e a análise das operações. A aplicação Android é disponibilizada às empresas responsáveis pelo transporte das encomendas e possibilita a actualização online da informação acerca do processo de entrega. O back-end é composto pela unidade de armazenamento e processamento da informação e encontra-se alojado num servidor com ligação à internet, disponibilizando uma interface com o serviço móvel do tipo serviço web. A concepção, desenvolvimento e descrição das funcionalidades desta ferramenta são abordadas ao longo do trabalho. Os testes realizados ao longo do desenvolvimento validaram o correcto funcionamento da ferramenta, estando pronta para a realização de um teste piloto.

Predictive value of serologic tests in the diagnosis and follow-up of patients with paracoccidioidomycosis

A serologic study was undertaken in a group of 43 patients with active paracoccidioidomycosis who were treated in the same form (ketoconazole), for identical periods of time (6 months), and folio wed-up for various periods posttherapy. The tests employed were agar gel immunodiffusion (AGID) and complement fixation (FC). Also studied were 50 sera from patients with proven histoplasmosis and pulmonary aspergilloma, 30 patients with culturaly proven tuberculosis as well as 92 specimens from healthy individuals, residents in the endemic area for paracoccidioidomycosis. A single lot of yeast filtrate antigen was used throughout the study. The value of each test was measured according to GALEN and GAMBINO6. Both tests were highly sensitive, 89 and 93% respectively. Regarding their specificity, the AGID was totally specific while the CF exhibited 96.6% and 97% specificity in front of tuberculosis patients and healthy individuals respectively and 82% in comparison with patients with other mycoses. The concept of predictive value, that is, the certainty one has in accepting a positive test as diagnostic of paracoccidioidomycosis, favored the AGID procedure (100%) over the CF test. The latter could sort out with 93% certainty a patient with paracoccidioidomycosis among a group of healthy individuals and with 97.5% in the case of TB patients; when the group in question was composed by individuals with other deep mycoses, such certainty was lower (81%). The above results indicate that both the AGID and the CF tests furnish results of high confidence; one should not relay, however, in the CF alone as a means to establish the specific diagnosis of paracoccidioidomycosis.

Desenvolvimento de recursos educativos em dispositivos móveis - RealHiBook

Nos últimos anos têm existido bastantes pesquisas e um grande investimento quer na área da educação à distância quer ao nível das tecnologias móveis. A dada altura percebeu-se que cada vez mais poderiam ser utilizados dispositivos móveis no contexto da educação e de que estes já seriam capazes de responder às exigências necessárias para que fosse possível criar ou melhorar cenários com recursos educativos. Este trabalho tem como objetivo propor um modelo que facilite a aprendizagem em dispositivos móveis. Nesse sentido, decidiu-se que seria uma mais-valia adicionar a este projeto, a utilização da Realidade Aumentada como potenciador de melhorias no processo de aprendizagem. A Realidade Aumentada, a par do que acontece com a educação à distância e com as tecnologias móveis, é uma área alvo de constantes investigações e inovação. Apresenta como principal capacidade a interação do utilizador com vários modelos virtuais incluídos numa cena real. Esta capacidade proporciona experiências que podem contribuir para o aumento da motivação e da perceção por parte dos alunos. A forma como o utilizador interage com as aplicações móveis é vista com extrema importância, principalmente na área da educação. Aliar uma boa interação à utilização da Realidade Aumentada, é parte integrante deste trabalho. Foram estudados exemplos de interações e formas como o utilizador vive a experiência durante a utilização de aplicações que partilham o mesmo tipo de tecnologias utilizadas neste projeto. O objetivo final foca-se na criação de uma aplicação, capaz de fornecer uma experiência enriquecedora no âmbito do ensino, ligado ao tema história e que consiga fundamentar a criação de um modelo que facilite a aprendizagem utilizando plataformas móveis. Os objetivos são corroborados por um conjunto de testes efetuados à aplicação e pelos resultados obtidos através de um questionário feito a um grupo de pessoas que testaram o protótipo.

Passive haemagglutination test for human neurocysticercosis immunodiagnosis: II - Comparison of two standardized procedures for the passive haemagglutination reagent in the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluids

A comparison of two different standardized reagent procedures for the passive haemagglutination test (PHA) in the detection of specific antibody to Cysticercus cellulosae in cerebrospinal fluid (CSF) was carried out. The formaldehyde-treated group O Rh-human red blood cells (HuRBC) and glutaraldehyde-treated sheep red blood cells (SRBC) were the supplies for the reagents preparation and, in the tests, they were designated as PHA-1 and PHA-2, respectively. For both reagents the cells were coated with the cysticerci total saline extract (TS) antigen. PHA-1 and PHA-2 were assessed in a total of 204 CSF from patients with neurocysticercosis, from non-related infections and from healthy individuals. The positivity and specificity indices obtained were respectively 81.7% and 94.4% for PHA-1 and for PHA-2, 88.7% and 96.6%. Since no significant differences were observed between the results provided by two reagents, at level of significance of 0.05, either processes of cell sensitization can alternatively be used according to the own laboratory convenience.

Application of a control banding tool for risk level assessment and control of nanoparticles exposure in welding operations

Scope of study: welding operations result in harmful emissions of nanoparticles; the aim of emissions monitorisation is to evaluate exposure levels and to derive protection measures in order to protect exposed workers; however, the traditional approach of comparing measured concentrations with exposure limits cannot be used; but risk levels can be quantified by using Control Banding Strategies.

Design and implementation of an autonomous, proactive, and reactive software infrastructure to help improving the management level of projects

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Engenharia Electrotécnica e de Computadores

Trends of T. cruzi infection based on data from blood bank screening

Between October 1988 and April 1989 a cross-sectional survey was carried out in six out of eight blood banks of Goiânia, Central Brazil. Subjects attending for first-time blood donation in the mornings of the study period (n = 1358) were interviewed and screened for T. cruzi infection as a part of a major study among blood donors. Tests to anti-T. cruzi antibodies were performed, simultaneously, by indirect hem agglutination test (IHA) and complement fixation test (CFT). A subject was considered seropositive when any one of the two tests showed a positive result. Information on age, sex, place of birth, migration and socio-economic level was recorded. Results from this survey were compared with seroprevalence rates obtained in previous studies in an attempt to analyse trend of T. cruzi infection in an endemic urban area. The overall seroprevalence of T. cruzi infection among first-time donors was found to be 3.5% (95% confidence interval 2.5%-4.5% ). The seroprevalence rate increased with age up to 45 years and then decreased. Migrants from rural areas had higher seroprevalence rates than subjects from urban counties (1.8%-16.2% vs. 0%-3.6%). A four fold decrease in prevalence rates was observed when these rates were compared with those of fifteen years ago. Two possible hypotheses to explain this difference were suggested: 1. a cohort effect related with the decrease of transmission in rural areas and/or 2. a differential proportion of people of rural origin among blood donors between the two periods. The potential usefulness of blood banks as a source of epidemiological information to monitor trends of T. cruzi infection in an urban adult population was stressed.

Comparison of indirect immunofluorescence test for measles antibodies with haemagglutination inhibition and plaque neutralization tests

Indirect Immunofluorescence (IFA), Plaque Reduction Neutralization (PRN) and Haemagglutination Inhibition (HI) tests for measles antibodies were carried out in 197 sera obtained from umbilical cord and vaccinated children. The IFA was also applied to blood samples collected with filter paper. IFA results demonstrated that the test is relatively simple to perform, with good reproducibility for different antigen lots. Good correlation was obtained between IFA, PRN and HI antibody titers. Better correlation was demonstrated with IFA and PRN than with HI and PRN tests. Sensitivity of IFA in detecting antibody was less effective than PRN, however more effective than HI using rhesus monkey red blood cells. PRN antibody titers over 100 were detected by IFA but not by HI (9.7% with negative results). IFA may be of considerable practical use and able to substitute HI in Seroepidemiological surveys and to evaluate vaccine efficacy. It also can be simplified by employing filter paper collected samples.

KM and politics at the highest level: an exploratory analysis

This paper analyzes Knowledge Management (KM) as a political activity, made by the great political leaders of the world. We try to inspect if at the macro political level KM is made, and how. The research is interesting because given that we live in a Knowledge society, in the Information Era, it is more or less obvious that the political leaders should also do KM. However we don’t know of any previous study on KM and world leaders and this paper wants to be a first step to fill that gap. As a methodology we use literature review: given this one is a first preliminary study we use data we found in the Internet and other databases like EBSCO. We divide the analysis in two main parts: theoretical ideas first, and an application second. The second part is it self divided in two segments: the past and the present times. We find that rather not surprisingly, KM always was and is pervasive in the activity of the world leaders, and has become more and more diverse has power itself became to be more and more disseminated in the world. The study has the limitation of relying on insights and texts and not on interviews. But we believe it is very interesting to make this kind of analysis and such studies may help improving the democracies in the world.

Including context in a routing algorithm for the internet of things

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Engenharia Informática

Enzyme-linked immunosorbent assay (ELISA) for measles antibody: a comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests

An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copo-sitivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.