Population structure in high shore littorinids: contrast between riprap and rocky shores

World Congress of Malacology, Ponta Delgada, July 22-28, 2013.

Efeitos da hidrodinâmica em comunidades biológicas de praias de calhau rolado em áreas marinhas protegidas

Jornadas "Ciência nos Açores – que futuro?", Ponta Delgada, 7-8 de Junho de 2013.

Effect of oxidative stress upon absorption of glucose by the human placenta: in vitro studies with BeWo cells

Pregnancy is a dynamic state and the placenta is a temporary organ that, among other important functions, plays a crucial role in the transport of nutrients and metabolites between the mother and the fetus, which is essential for a successful pregnancy. Among these nutrients, glucose is considered a primary source of energy and, therefore, fundamental to insure proper fetus development. Several studies have shown that glucose uptake is dependent on several morphological and biochemical placental conditions. Oxidative stress results from the unbalance between reactive oxygen species (ROS) and antioxidants, in favor of the first. During pregnancy, ROS, and therefore oxidative stress, increase, due to increased tissue oxygenation. Moreover, the relation between ROS and some pathological conditions during pregnancy has been well established. For these reasons, it becomes essential to understand if oxidative stress can compromise the uptake of glucose by the placenta. To make this study possible, a trophoblastic cell line, the BeWo cell line, was used. Experiments regarding glucose uptake, either under normal or oxidative stress conditions, were conducted using tert-butylhydroperoxide (tBOOH) as an oxidative stress inducer, and 3H-2-deoxy-D-glucose (3H-DG) as a glucose analogue. Afterwards, studies regarding the involvement of glucose facilitative transporters (GLUT) and the phosphatidylinositol 3-kinases (PI3K) and protein kinase C (PKC) pathways were conducted, also under normal and oxidative stress conditions. A few antioxidants, endogenous and from diet, were also tested in order to study their possible reversible effect of the oxidative effect of tBOOH upon apical 3H-DG uptake. Finally, transepithelial studies gave interesting insights regarding the apical-to-basolateral transport of 3H-DG. Results showed that 3H-DG uptake, in BeWo cells, is roughly 50% GLUT-mediated and that tBOOH (100 μM; 24h) decreases apical 3H-DG uptake in BeWo cells by about 33%, by reducing both GLUT- (by 28%) and non-GLUT-mediated (by 40%) 3H-DG uptake. Uptake of 3H-DG and the effect of tBOOH upon 3H-DG uptake are not dependent on PKC and PI3K. Moreover, the effect of tBOOH is not associated with a reduction in GLUT1 mRNA levels. Resveratrol, quercetin and epigallocatechin-3-gallate, at 50 μM, reversed, by at least 45%, the effect of tBOOH upon 3H-DG uptake. Transwell studies show that the apical-to-basolateral transepithelial transport of 3H-DG is increased by tBOOH.In conclusion, our results show that tBOOH caused a marked decrease in both GLUT and non-GLUT-mediated apical uptake of 3H-DG by BeWo cells. Given the association of increased oxidative stress levels with several important pregnancy pathologies, and the important role of glucose for fetal development, the results of this study appear very interesting.

Modelling the distribution of cetaceans using opportunistic presence-only data in S. Miguel Island (Azores)

25th Annual Conference of the European Cetacean Society, Cadiz, Spain 21-23 March 2011.

Deep ocean species. The little that is known mostly comes from collected specimens. Comment on L.A. Rocha et al. Letter "Specimen collection: An essential tool" (23 May, 344: 814).

Deep Ocean Species. The little that is known mostly comes from collected specimens. L.A. Rocha et al. Letter "Specimen collection: An essential tool" (23 May, 344: 814) brilliantly discuss the importance of specimen collection and present the evolution of collecting since the mid-19th century until our present strict codes and conducts. However, it is also important to emphasize the fact that the vast majority of deep ocean macro-organisms are only known to us because of collection and this is a strong argument that should be present in our actions as scientists. If the deep is considered the least known of Earth’s habitats (1% or so according to recent estimates) then what awesome collection of yet to discover species are still there to be properly described? As the authors point citing (1), something around 86% of species remain unknown. Voucher specimens are fundamental for the reasons pointed out and perhaps the vast depths of the World’s oceans are the best example of that importance. The resumed report of 2010 Census of Marine Life (2) showed that among the millions of specimens collected in both familiar and seldom-explored waters, the Census found more than 6,000 potentially new species and completed formal descriptions of more than 1,200 of them. It also found that a number of rare species are in fact common. Voucher specimens are essential and, again agreeing with L.A. Rocha et al. Letter (see above), the modern approach for collecting will not be a cause for extinctions but instead a valuable tool for knowledge, description and even, as seen above, a way to find out that supposed rare species may not be that rare and even prove to reach abundant populations.

A comparison of bryophyte diversity in the Macaronesian Islands: island versus habitat approach

In this paper, we present a comparison of richness patterns and floristic similarity for bryophytes in the five most important altitudinal habitat types in the Macaronesian islands. We evaluate the importance of different factors discussed in the literature in predicting species diversity applying the traditional island approach and within the framework of the new habitat approach, including area, isolation, climatic factors, geological age and human influence. From the analysis of patterns of bryophyte species distribution for selected habitats across islands and archipelagos, we specifically test the hypothesis that (i) floristic similarity is primarily determined by climatic factors, but not by geographical distance due to high dispersal ability in this species group and (ii) bryophyte richness is best predicted by area, but not by geological age of the habitat due to very low endemicity or speciation rate and high colonization rate.

Bryophytes distribution along an altitudinal gradient of native forest in Pico island (Azores): preliminary results of epiphytic genera

MOVECLIM, Mid Course Meeting, 2-6 September 2013, Réunion (Mascarenes).

Biology, ecology and conservation of mobulid rays in the Azores

Dissertação de Mestrado, Estudos Integrados dos Oceanos, 12 de Dezembro de 2013, Universidade dos Açores.

Contribuição para o estudo das comunidades zooplanctónicas das lagoas dos Açores

Dissertação de Mestrado, Biodiversidade e Ecologia Insular, 4 de Abril de 2014, Universidade dos Açores.

Opções reais: Avaliação do projecto de investimento de três parques eólicos em ambiente onshore e offshore

Os Projectos de Investimento desempenham um importante papel no crescimento económico-social dos países, proporcionando emprego e desenvolvimento tecnológico. Na óptica dos projectos inovadores, concretamente no sector das energias renováveis, acarretam elevados investimentos, numa base temporal de longo prazo. Nestes casos as decisões estratégicas assumem um papel determinante, assim, o principal objectivo desta dissertação é a utilização das Opções Reais como métrica de avaliação dos projectos de investimento. A análise e avaliação dos projectos implica em si incerteza nas previsões, desta forma, as Opções Reais minimizam o risco associado à incerteza através da inclusão da flexibilidade no processo de avaliação. A primeira parte da dissertação consiste na contextualização energética mundial e nacional, ao nível da energia primária e das energias renováveis, com incidência na energia eólica. A segunda consiste na introdução teórica dos projectos de investimento e dos conceitos inerentes às Opções Financeiras e às Opções Reais. Por último, apresenta-se um caso de estudo de construção de três parques eólicos e as consequentes decisões de investimento concluindo que os modelos de avaliação das Opções Reais proporcionam alternativas e interdependência em investimentos futuros.

Deep-Sea ecosystem model of the Condor seamount

Dissertação de Mestrado, Estudos Integrados dos Oceanos, 25 de Julho 2013, Universidade dos Açores.

O complexo Myosotella 'myosotis/denticulata': uma perspetiva morfológica, molecular e ecológica

Dissertação de Mestrado, Biodiversidade e Ecologia Insular, 29 de Maio de 2013, Universidade dos Açores.

Role of the wider Azores region as a nursery ground for North Atlantic blue shark (Prionace glauca)

Tese de Doutoramento, Ciências do Mar (Ecologia Marinha)

Mapping multi-species habitat use for marine conservation planning

Tese de Doutoramento, Ciências do Mar (Biologia Marinha)

Assessing the conservation status of the strict endemic Desertas wolf spider, Hogna ingens (Araneae, Lycosidae)

The Desertas Islands (Madeira, Portugal) are the sole home of one of the largest and rarest wolf spiderspecies, Hogna ingens (Blackwall 1857) (Araneae, Lycosidae). Despite its size, it inhabits a single valleyin the North of the Deserta Grande Island, Vale da Castanheira, currently invaded by the herb Phalarisaquatica. This invasive species competes with the native flora and was subject to several eradicationexperiments, namely through fire and chemicals. The objectives of this work were to: (1) estimate thecurrent distribution and abundance of H. ingens and respective trends; (2) evaluate the impact of theinvasive plant and eradication methods on the spider population; (3) suggest future measures for therecovery of the species; and (4) evaluate its conservation status according to the IUCN criteria. The current distribution of H. ingens covers 23 ha, a recent reduction from its original 83 ha, correspond-ing to the entire Vale da Castanheira. A total of 4447 and 4086 adults and 71,832 and 24,635 juvenileswere estimated to live in the valley during 2011 and 2012, respectively. We found a significant negativeimpact of P. aquatica cover on the presence and abundance of H. ingens and that chemical treatmentspecifically directed towards the invasive plant species may be the only way to effectively recover thespider's habitat. We suggest (1) regular monitoring; (2) extend chemical treatments; (3) ex-situ conserva-tion with future reintroduction of adults. Based on the current area of occupancy (AOO) of H. ingens and itsrecent decline in both AOO and number of individuals, it was recently classified as Critically Endangeredby IUCN and we suggest its urgent inclusion in the Habitats Directive species lists.