Análise do potencial de aprendizagem em ambiente de sala de espera. Proposta de protótipo

Sempre que temos necessidade de nos deslocarmos a uma instituição de saúde para uma consulta ou para um exame clínico, somos sistematicamente obrigados a esperar num local para o efeito onde se encontram, por norma, outros utentes e/ou pacientes em espera. Este local é tradicionalmente denominado como sala de espera, na qual são normalmente disponibilizadas revistas e jornais com o intuito de proporcionar aos utentes alguma distração enquanto aguardam pela consulta. Este tempo de espera costuma provocar e despoletar um “turbilhão” de sensações durante esse período, que podem mesmo chegar a ser antagónicas. Estes espaços de espera têm evoluído verificando-se a inclusão de elementos tecnológicos, com o intuito de tornar o “ato de espera” menos pesado e mais agradável para os seus utentes. A inclusão de televisões evidenciou uma evolução no processo de descontração do paciente em espera, passando de um espaço onde inicialmente a informação e/ou meios de distração se baseava apenas em revistas ou jornais. De acordo com a evidente evolução tecnológica e a banalização do acesso à Internet, pretendeu-se investigar a possibilidade de proporcionar, aos utentes em espera, uma aprendizagem (ainda que momentânea) através da transmissão de informação útil sobre a área da saúde (informação de carácter geral ou específico). Para o efeito, analisou-se a possibilidade deste tipo de espaços poderem usufruir de equipamentos tecnológicos como, por exemplo, computadores ou ecrãs informativos interativos e aferir as facilidades de acesso à Internet. Neste âmbito, procurou-se ainda evidenciar e demonstrar a importância do espaço da sala de espera, analisando quais as suas principais funções e como são atualmente utilizadas. A análise a este tipo de espaço e às suas caraterísticas permitiu efetuar uma caracterização de possíveis tipologias de sala de espera. Para além disso, efetuou-se um estudo para analisar de que forma se poderia dinamizar este espaço, tendo sempre presente o paciente em espera como centro de atenções, visto que é ele o principal utilizador deste tipo de espaço. Posteriormente idealizou-se e desenvolveu-se um protótipo, com o intuito de investigar a possibilidade de proporcionar a transmissão de conhecimento adicional (“educar”) através da transmissão de informação útil aos pacientes (por exemplo, sobre tratamentos e/ou doenças), difundindo-a nos ecrãs disponíveis na sala de espera. Por fim, avaliou-se o seu potencial para validar se é possível e útil aprender durante a espera.

Monitorização e caracterização de atividade física

A monitorização da atividade física é um tema que tem adquirido cada vez mais importância. Tal deve-se ao crescente sedentarismo da população em geral e adquirindo níveis muito elevados de importância devido a vários fatores como por exemplo o enorme crescimento tecnológico e menor tempo de lazer. Cada vez mais a população tem a tendência de substituir atividades como uma simples caminhada para o trabalho ou escola por algum tipo de tecnologia que reduz o consumo energético do corpo, sendo paradigmático o uso (excessivo) de viaturas automóveis. Em consequência da escassez de atividade física, doenças como a obesidade e problemas cardíacos têm vindo a aumentar nas várias faixas etárias, mas assume uma particular relevância em crianças. Nas últimas décadas têm aumentado as iniciativas de investigação com o objetivo de compreender os fatores que afetam a prática de atividade física para posteriormente a potenciar. Existem diversos métodos contudo, destaca-se preferencialmente os de observação direta, com observadores presentes. No entanto estes apresentam algumas limitações. Consequentemente são necessários esforços de investigação adicionais e novas técnicas ou metodologias. Nesta dissertação pretende-se contribuir ativamente para a investigação na área da promoção de atividade física através da utilização de vídeo, com uma análise realizada sobre dois pontos principais. Primeiro são analisadas métodos do estado de arte que requerem a presença de observadores e de que forma a captura de vídeos pode ser utilizada como alternativa ou complemento. De seguida, é realizado um estudo e avançada uma proposta inicial para utilizar mecanismos de processamento e classificação automática da atividade em alternativa ao observador humano.

Characterization of Nitric Oxide Reductase (NOR) from pseudomonas nautica, a study on biologic nitric oxide reduction

Dissertation submitted to obtain the phD degree in Biochemistry, specialty in Physical- Biochemistry, by the Faculdade de Ciências e Tecnologia from the Universidade Nova de Lisboa

Characterization of molybdenum and tungsten formate dehydrogenases from sulfate reducing bacteria

Dissertação apresentada para a obtenção do Grau de Doutor em Bioquímica, especialidade Bioquímica-Física pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection

In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively.

Phenotypic and molecular characterization of Salmonella Enteritidis strains isolated in São Paulo, Brazil

In São Paulo State, Brazil, the epidemic increase in isolation of Salmonella Enteritidis has been observed since 1994. A total of 105 S. Enteritidis strains (72 from human and 33 from non-human sources) isolated during the period 1975-1995, previously characterized by phage typing, was analyzed by antimicrobial susceptibility, plasmid profile, and ribotyping. Over 70% of the strains were susceptible to all antimicrobial agents tested, however, multiple resistance to antimicrobials was observed among the studied strains, mainly those from hospitalized patients. Phage type 8 (PT-8) was predominant among the strains isolated during the period of 1975-1992, but in the following years, PT-4 was the most frequent phage type identified. Seven different plasmid profiles were detected and 96% of the isolates harbored a plasmid of approximately 36 MDa. Ribotyping discriminated fourteen ribotypes (R1 to R14) among the strains examined. By analysis of dendrogram the strains were included in three groups with similarity level of 60%. The obtained results indicate that, a single ribotype (R11), determined for PT-4 strains isolated from 1993, characterizes the epidemic clone of S. Enteritidis in our region.

Characterization of the portuguese SSS into the Europe: a contribution

Nowadays, Short Sea Shipping (SSS) is an essential part in European multi-modal transport system, representing approximately thirty-seven per cent of intra-Community transactions in tonnes per kilometre (tkm). Since 2001, the European Shortsea Network (ESN) in partnership with the short-sea Promotion Centres (SPC) of each Member State of the European Union (EU) have managed to make significant progress in the promotion and development of this mode of transport. This paper aims to assess and analyse the SSS of containerised goods in Portugal and its articulation with other EU routes and also other transport modes. The current SSS infrastructure, how the sector is organized, as well as the future perspectives for the sector are also analysed for the case of Portugal. The analyses are based on a survey that was carried out on the logistics operators, navigation agents, freight forwarders, and the leading imports and exports manufacturers in Portugal.

Functional and molecular characterization of SR45, a plant-specific factor involved in sugar and stress signaling in Arabidopsis thaliana

Dissertation presented to obtain the Ph.D degree in Molecular Biology

Barnacle Allergy: Allergen Characterization and Cross-Reactivity with Mites

Background: Barnacles are a type of seafood with worldwide distribution and abundant along the shores of temperate seas. They are particularly appreciated and regularly consumed in Portugal as well as in Spain, France and South America, but barnacle allergy is a rare condition of which there is only one reference in the indexed literature. The molecular allergens and possible cross-reactivity phenomena implicated (namely with mites) have not been established. Objective: To demonstrate the IgE-mediated allergy to barnacle and to identify the proteins implicated as well as possible cross-reactivity phenomena with mites. Methods: We report the clinical and laboratory data of five patients with documented IgE-mediated allergy to barnacle. The diagnosis was based on a suggestive clinical history combined with positive skin prick tests (SPT) to barnacle – prick to prick method. Two barnacle extracts were prepared (raw and cooked barnacle) and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting were performed. An immunoblotting inhibition assay with Dermatophagoides pteronyssinus was also done in order to evaluate cross-reactivity. Results: All patients had mite-related asthma and the allergic rhinoconjunctivitis; they all experienced mucocutaneous symptoms. All of them had positive SPT to barnacle, and the immunoblotting showed several allergenic fractions with a wide molecular weight range (19 – 94 kDa). The D. pteronyssinus extract inhibited several IgE-binding protein fractions in the barnacle extract. Conclusions: We describe five patients with IgE-mediated barnacle allergy. We also describe a group of IgEbinding+ proteins between 30 and 75 kDa as the allergenic fractions of this type of Crustacea. Cross-reactivity with D. pteronyssinus was demonstrated in two cases.

A Real Time Vision System for Autonomous Systems: Characterization during a Middle Size Match

in RoboCup 2007: Robot Soccer World Cup XI

Purification and Preliminary Characterization of Tetraheme Cytochrome and Adenylylsulf te Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254

Bioinorganic Chemistry and Applications Volume 3 (2005), Issue 1-2, Pages 81-91

Characterization of Cryptococcus neoformans isolated from urban environmental sources in Goiânia, Goiás State, Brazil

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis as the most frequent clinical presentation in immunocompromised patients, mainly in people infected by HIV. This fungus is an environmental encapsulated yeast, commonly found in soil enriched with avian droppings and plant material. A total of 290 samples of pigeon and the other avian droppings, soil, ornamental trees and vegetable material associated with Eucalyptus trees were collected to study environmental sources of Cryptococcus species in Goiânia, Goiás State. The determination of varieties, serotypes and the susceptibility in vitro to fluconazole, itraconazole and amphotericin B of C. neoformans isolates were performed. C. neoformans var. grubii (serotype A) was found in 20.3% (36/177) of pigeon dropping samples and in 14.3% (5/35) of samples of Eucalyptus. None of the environmental isolates of C. neoformans showed in vitro resistance to three antifungal agents. The knowledge of major route for human cryptococcal infection (inhalation of infectious particles from saprophytic sources) and a total of 60 C. neoformans isolates obtained from AIDS patients with cryptococcal meningitis between October 2001 and April 2002 justify the study of the habitats of these yeasts as probable sources of cryptococcosis in this city.

A technological innovation plan for enterprises in the Azores

The INOTEC-Empresa – the Technological Innovation Plan for Enterprises in the Autonomous Region of the Azores (RAA) - was developed in 2006-2007, at the request of the Regional Government, with the main aim of promoting innovation within small and medium enterprises. The methodological approach used in the development of the INOTEC – Empresa Plan was designed to obtain a comprehensive view of regional actors and included a document review, participation of the various actors through interviews, a collection of statements from RAA – Região Autónoma dos Açores – entrepreneurs, academics, public leaders and other key players, together with an analysis of their views and a survey of the innovation dynamics of the most relevant Azorean enterprises. The INOTEC-Empresa – the Technological Innovation Plan for Enterprises – comprises seven programmes aimed at promoting innovation in the Region. This paper focuses on the Programmes for Qualification of Human Resources and the Development of Scientific and Technological Capacities for Innovation. Some socio-economic data and the metrics selected to assess and benchmark the implementation of the Plan will als