Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection
Data(s) |
01/01/2003
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Resumo |
In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively. |
Formato |
text/html |
Identificador |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000100003 |
Idioma(s) |
en |
Publicador |
Instituto de Medicina Tropical |
Fonte |
Revista do Instituto de Medicina Tropical de São Paulo v.45 n.1 2003 |
Palavras-Chave | #Dengue virus #Dual infection #RT/PCR #E/NS1 gene sequences #Phylogenetic analysis |
Tipo |
journal article |