Atributos químicos no solo e produção de Cynodon dactylon cv. Tierra Verde sob doses de biofertilizante orgânico

The concentration of nutrients in plant is related to the soil, fertilization, climate, season, cultivar and cultural practices. Aiming to evaluate the soil chemical properties, the dry matter production of shoots and roots, nutrient content in the plant and the chemical composition of the grass Tierra Verde subjected to levels of organic biofertilizer as nitrogen source, an experiment was conducted in a greenhouse at the Faculty of Veterinary Medicine , UNESP, Araçatuba-SP, January-September 2010. Treatments were arranged in a completely randomized design with six fertilized biofertilizer doses (0, 33, 66, 132, 264, 528 m3 ha-1) and five repetitions for three cuts. We used the model split plot, with doses of biofertilizer considered as main treatments and cuts as sub-plots. We obtained a linear response in the production of dry mass of shoots and roots to the dose of 528 m3 ha-1 of organic biofertilizer. Nitrogen fertilization influenced the soil chemical properties and levels of organic matter, sulfur, boron and manganese, and in foliar levels of phosphorus, potassium and copper. The chemical composition was not altered by the influence of organic biofertilizer doses applied to the soil.

Effect of antioxidants during bovine in vitro fertilization procedures on spermatozoa and embryo development

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Expression of α6 integrin subunit in bovine oocyte and its potential role during fertilization

Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.

Disponibilidade de n e k no solo e sua absorção pela videira de vinho fertirrigada no semiárido

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Avaliação da qualidade de alimentos biofortificados produzidos em sistemas agroecológicos do trópico úmido

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Acúmulo de nutrientes e resposta da alface à adubação fosfatada

Nutrient absorption and response of lettuce to phosphorus fertilization. The objective of this study was to evaluate the response of lettuce (cultivar Verônica) to different levels of phosphorus fertilization. The experiment was conducted from 25/09/2003 (sowing) to 03/12/2003 (harvesting) at the Fazenda Experimental São Manuel, UNESP/FCA, Botucatu/SP, under protective structures. The experiment was conducted in a randomized block design, with five treatments (0, 200, 400, 600 and 800kg.ha-1 of P2 O5, in the form of triple superphosphate) and five replications. Plants were grown in 13L plastic pots containing Latossolo Vermelho Distrófico Típico. A quadratic response was observed for the fresh mass of the plant shoots and leaf area, with maximum equivalent levels of 733 and 756kg.ha-1 of P2 O5 , respectively, as well as for potassium, calcium, magnesium, sulfur, boron, copper, iron, manganese and zinc accumulation. Linear increases were observed with the treatments of P2 O5 for dry mass of the shoots, leaf number, plant height and nitrogen and phosphorus accumulation.

Coffee processing residues as a soil potassium amendment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise Espacial Multivariada Aplicada na Avaliação da Fertilidade do Solo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento da grama Tifton 419 submetida a distintos manejos de adubação e irrigação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of potassium silicate on epidemic components of powdery mildew on melon

The effects of silicon (Si) supplied in the form of potassium silicate (PS) were evaluated on epidemic components of powdery mildew of melon under greenhouse conditions. The PS was applied to the roots or to leaves. In the first experiment, epidemic components were evaluated after inoculation with Podosphaera xanthii. In the second experiment, the disease progress rate was evaluated on plants subjected to natural infection. The area under the disease progress curve was reduced by 65% and 73% in the foliar and root treatments, respectively, compared to control plants, as a consequence of reductions in infection efficiency, colony expansion rate, colony area, conidial production and disease progress rate. However, root application of PS was more effective than foliar application in reducing most of the epidemic components, except for infection efficiency. This can be explained by the high Si concentration in leaf tissues with root application, in contrast to the foliar treatment where Si was only deposited on the external leaf surfaces. The effects of PS reported in this study demonstrated that powdery mildew of melon can be controlled, and that the best results can be achieved when PS is supplied to the roots.

Determination of relative in vivo osteoconductivity of modified potassium fluorrichterite glass-ceramics compared with 45S5 bioglass

Potassium fluorrichterite (KNaCaMg5Si8O22F2) glass-ceramics were modified by either increasing the concentration of calcium (GC5) or by the addition of P2O5 (GP2). Rods (2 x 4 mm) of stoichiometric fluorrichterite (GST), modified compositions (GC5 and GP2) and 45S5 bioglass, which was used as the reference material, were prepared using a conventional lost-wax technique. Osteoconductivity was investigated by implantation into healing defects in the midshaft of rabbit femora. Specimens were harvested at 4 and 12 weeks following implantation and tissue response was investigated using computed microtomography (mu CT) and histological analyses. The results showed greatest bone to implant contact in the 45S5 bioglass reference material at 4 and 12 weeks following implantation, however, GST, GC5 and GP2 all showed direct bone tissue contact with evidence of new bone formation and cell proliferation along the implant surface into the medullary space. There was no evidence of bone necrosis or fibrous tissue encapsulation around the test specimens. Of the modified potassium fluorrichterite compositions, GP2 showed the greatest promise as a bone substitute material due to its osteoconductive potential and superior mechanical properties.

Magmatism and fenitization in the Cretaceous potassium-alkaline-carbonatitic complex of Ipanema So Paulo State, Brazil

The Ipanema alkaline-carbonatitic complex is part of the Meso-Cenozoic alkaline magmatism located within the southeastern part of the Brazilian Platform. Drill-core and field sampling have indicated the occurrence of glimmerites, with subordinate shonkinites (mela-syenites), clinopyroxene-bearing glimmerites, diorites and syenites. The glimmerites are cross-cut by lamprophyric dykes and calciocarbonatites. Fenitization has deeply affected the country rocks, originating dioritic and syenitic rocks. The Ipanema rocks show a distinct potassic affinity. The initial Sr-Nd- isotopic composition of the Ipanema rocks ((87)Sr/(86)Sr = 0.70661-0.70754 and (143)Nd/(144)Nd = 0.51169-0.51181) is similar to that of tholeiitic and potassium-rich-alkaline rocks of the Eastern Paraguay. Stable isotope data for the Ipanema calciocarbonatite suggest interaction with fluids at temperatures typical of hydrothermal stages, as hypothesized for other carbonatite complexes from southeastern Brazil. The chemical differences between the lamprophyre, glimmerites, carbonatites, apatitites and magnetitites, and the absence of marked REE enrichment in the evolved lithologies, all indicate that fractional crystallization and accumulus of liquidus phases in a magma reservoir, likely coupled with liquid immiscibility processes, may have played an important role in the genesis of the Ipanema rocks.

Diverse levels of an inwardly rectifying potassium conductance generate heterogeneous neuronal behavior in a population of dorsal cochlear nucleus pyramidal neurons

Leao RM, Li S, Doiron B, Tzounopoulos T. Diverse levels of an inwardly rectifying potassium conductance generate heterogeneous neuronal behavior in a population of dorsal cochlear nucleus pyramidal neurons. J Neurophysiol 107: 3008-3019, 2012. First published February 29, 2012; doi:10.1152/jn.00660.2011.-Homeostatic mechanisms maintain homogeneous neuronal behavior among neurons that exhibit substantial variability in the expression levels of their ionic conductances. In contrast, the mechanisms, which generate heterogeneous neuronal behavior across a neuronal population, remain poorly understood. We addressed this problem in the dorsal cochlear nucleus, where principal neurons exist in two qualitatively distinct states: spontaneously active or not spontaneously active. Our studies reveal that distinct activity states are generated by the differential levels of a Ba2+-sensitive, inwardly rectifying potassium conductance (K-ir). Variability in K-ir maximal conductance causes variations in the resting membrane potential (RMP). Low K-ir conductance depolarizes RMP to voltages above the threshold for activating subthreshold-persistent sodium channels (Na-p). Once Na-p channels are activated, the RMP becomes unstable, and spontaneous firing is triggered. Our results provide a biophysical mechanism for generating neural heterogeneity, which may play a role in the encoding of sensory information.

Kaurenoic Acid from Sphagneticola trilobata Inhibits Inflammatory Pain: Effect on Cytokine Production and Activation of the NO-Cyclic GMP-Protein Kinase G-ATP-Sensitive Potassium Channel Signaling Pathway

Kaurenoic acid [ent-kaur-16-en-19-oic acid (1)] is a diterpene present in several plants including Sphagneticola trilobata. The only documented evidence for its antinociceptive effect is that it inhibits the writhing response induced by acetic acid in mice. Therefore, the analgesic effect of 1 in different models of pain and its mechanisms in mice were investigated further. Intraperitoneal and oral treatment with 1 dose-dependently inhibited inflammatory nociception induced by acetic acid. Oral treatment with 1 also inhibited overt nociception-like behavior induced by phenyl-p-benzoquinone, complete Freund's adjuvant (CFA), and both phases of the formalin test. Compound 1 also inhibited acute carrageenin- and PGE(2)-induced and chronic CFA-induced inflammatory mechanical hyperalgesia. Mechanistically, 1 inhibited the production of the hyperalgesic cytokines TNF-alpha and IL-1 beta. Furthermore, the analgesic effect of 1 was inhibited by L-NAME, ODQ, KT5823, and glybenclamide treatment, demonstrating that such activity also depends on activation of the NO-cyclic GMP-protein kinase G-ATP-sensitive potassium channel signaling pathway, respectively. These results demonstrate that 1 exhibits an analgesic effect in a consistent manner and that its mechanisms involve the inhibition of cytokine production and activation of the NO-cyclic GMP-protein lcinase G-ATP-sensitive potassium channel signaling pathway.

Nutrição mineral da cana‑de‑açúcar irrigada com efluente de esgoto tratado, em área com aplicação de fosfogesso

O objetivo deste trabalho foi quantificar o aporte e a remoção de nutrientes em sistemas de cultivo  de cana‑de‑açúcar irrigados, ou não, com efluente de estação de tratamento de esgoto (EETE), com e sem  adição  de fosfogesso,  bem  como  avaliar  os  efeitos  desses sistemas  de  cultivo  no  estado  nutricional  das  plantas. Foram avaliados tratamentos sem irrigação e com irrigação a 100 e 150% da necessidade hídrica da  cultura. Os tratamentos com fosfogesso foram aplicados em área de terceiro corte, irrigada com EETE desde  o plantio. As  avaliações foram realizadas em duas safras. Os tratamentos não afetaram os rendimentos de  colmos. O tratamento com EETE e fosfogesso apresentou efeito sinérgico sobre o conteúdo de nitrogênio e de  enxofre nas plantas. O EETE beneficiou a nutrição das plantas quanto ao fósforo, mas não causou melhorias  na nutrição com potássio e enxofre. A nutrição com ferro, zinco e manganês não foi influenciada pelo aporte  desses micronutrientes pelo EETE. O  fósforo e o nitrogênio aportados na irrigação com EETE devem ser  considerados na recomendação de adubação. Porém, potássio, enxofre, ferro, zinco e manganês do efluente não  são fontes eficientes desses nutrientes para as plantas.