Multi-distributed wireless sensors for monitoring a long distance transport in a reefer container

The study of the temperature gradients in cold stores and containers is a critical issue in the food industry for the quality assurance of products during transport and for minimising losses. This work presents an analysis of the temperatures during the refrigerated transport of 4,320 kg of blueberries in a reefer (set point temperature at ?1ºC) on a container ship from Montevideo (Uruguay) to Verona (Italy). The monitoring was performed by using semi-passive RFID loggers (TurboTag cards). The objective was to carry out a multi-distributed supervision using low-cost, wireless and autonomous sensors for the characterisation of the distribution and spatial gradients of temperatures during a long distance transport. Data analysis shows spatial (phase space) and temporal sequencing diagrams and reveals a significant heterogeneity of temperature at different locations in the container, which highlights the ineffectiveness of a temperature control system based on a single sensor, as is usually done.

Snapshot isolation for Neo4j

NoSQL data stores are becoming more and more popular. Graph databases are one of this kind of data stores. In this paper we present an overview of the implementation of snapshot isolation for Neo4j, a very popular graph database.

Monitorización inteligente de redes de comunicación

El avance tecnológico de los últimos años ha aumentado la necesidad de guardar enormes cantidades de datos de forma masiva, llegando a una situación de desorden en el proceso de almacenamiento de datos, a su desactualización y a complicar su análisis. Esta situación causó un gran interés para las organizaciones en la búsqueda de un enfoque para obtener información relevante de estos grandes almacenes de datos. Surge así lo que se define como inteligencia de negocio, un conjunto de herramientas, procedimientos y estrategias para llevar a cabo la “extracción de conocimiento”, término con el que se refiere comúnmente a la extracción de información útil para la propia organización. Concretamente en este proyecto, se ha utilizado el enfoque Knowledge Discovery in Databases (KDD), que permite lograr la identificación de patrones y un manejo eficiente de las anomalías que puedan aparecer en una red de comunicaciones. Este enfoque comprende desde la selección de los datos primarios hasta su análisis final para la determinación de patrones. El núcleo de todo el enfoque KDD es la minería de datos, que contiene la tecnología necesaria para la identificación de los patrones mencionados y la extracción de conocimiento. Para ello, se utilizará la herramienta RapidMiner en su versión libre y gratuita, debido a que es más completa y de manejo más sencillo que otras herramientas como KNIME o WEKA. La gestión de una red engloba todo el proceso de despliegue y mantenimiento. Es en este procedimiento donde se recogen y monitorizan todas las anomalías ocasionadas en la red, las cuales pueden almacenarse en un repositorio. El objetivo de este proyecto es realizar un planteamiento teórico y varios experimentos que permitan identificar patrones en registros de anomalías de red. Se ha estudiado el repositorio de MAWI Lab, en el que se han almacenado anomalías diarias. Se trata de buscar indicios característicos anuales detectando patrones. Los diferentes experimentos y procedimientos de este estudio pretenden demostrar la utilidad de la inteligencia de negocio a la hora de extraer información a partir de un almacén de datos masivo, para su posterior análisis o futuros estudios. ABSTRACT. The technological progresses in the recent years required to store a big amount of information in repositories. This information is often in disorder, outdated and needs a complex analysis. This situation has caused a relevant interest in investigating methodologies to obtain important information from these huge data stores. Business intelligence was born as a set of tools, procedures and strategies to implement the "knowledge extraction". Specifically in this project, Knowledge Discovery in Databases (KDD) approach has been used. KDD is one of the most important processes of business intelligence to achieve the identification of patterns and the efficient management of the anomalies in a communications network. This approach includes all necessary stages from the selection of the raw data until the analysis to determine the patterns. The core process of the whole KDD approach is the Data Mining process, which analyzes the information needed to identify the patterns and to extract the knowledge. In this project we use the RapidMiner tool to carry out the Data Mining process, because this tool has more features and is easier to use than other tools like WEKA or KNIME. Network management includes the deployment, supervision and maintenance tasks. Network management process is where all anomalies are collected, monitored, and can be stored in a repository. The goal of this project is to construct a theoretical approach, to implement a prototype and to carry out several experiments that allow identifying patterns in some anomalies records. MAWI Lab repository has been selected to be studied, which contains daily anomalies. The different experiments show the utility of the business intelligence to extract information from big data warehouse.

Un nuevo equipamiento territorial : los parques comerciales : criterios y parámetros de diseño : análisis de la experiencia española

La revolución industrial, en todas sus etapas, supuso un cambio que afectó de lleno a la actividad comercial en la ciudad, produciéndose paulatinamente, una separación radical entre los lugares de producción de bienes manufacturados y los de intercambio. Estos últimos, van especializándose, desde las primeras agrupaciones de comercios en pequeños mercados, hasta una de las formas más evolucionadas de superficie comercial en la actualidad, los Parques Comerciales. En base a que actualmente, no hay estudios concluyentes sobre la manera de abordar estos nuevos espacios, se ha considerado el estudio de 17 Parques Comerciales representativos españoles, de este se han obtenido datos según su planeamiento, de los cuales se ha postulado una división de los Parques Comerciales en los que tienen ordenación exclusiva y los que se han desarrollado sin ordenación exclusiva, de la misma manera, se han dividido cada uno de ellos en los que se considera parcela de aparcamiento y en los que la parcela de aparcamiento está incluida en la parcela a edificar, de este manera se han obtenido unos parámetros de dimensionamiento representativos para cada uno de los casos considerados, que van a servir de orientación para el responsable de futuros planeamientos, tales como usos, parámetros de ordenación, distribución urbanística, aparcamientos, etc. También se realiza una clasificación de los Parques Comerciales en función de las dimensiones de las "medianas comerciales" que los corforman. La Tesis postula una nueva definición de Parque Comercial más completa y adaptada a la importancia de este formato comercial, así como unas recomendaciones para su diseño y desarrollo en base a los parámetros urbanísticos obtenidos. Se desarrolla en la Tesis el horizonte previsible de los Parques Comerciales y las posibles nuevas líenas de investigación. The industrial revolution, in all its stages, marked a change that would have a major impact on commercial activity in cities, gradually producing a radical separation between the production sites of manufacturated goods and those of trading goods. The latter are becoming increasingly specialized, from the first trade associations in small markets, up to what is now one of the most highly evolved forms of retail spaces, the Commercial Parks. On the grounds that there are currently no conclusive studies on the manner in which to address these new spaces, a study of 17 prominent Spanish Commercial Parks has been considered, from which data has been obtained based on the way in which they were planned. It has been proposed that these Commercial Parks be divided into those that have been developed without exclusive planning. Likewise, each of these has been divided into those considered as parking lots and those in which the parking area is included in the building plot. In this manner, representative sizing parameters have been obtained for each of the cases considered, which will act as guidelines for the person in charge of future proposals, such as uses, development parameters, town planning, parking, etc. The Commercial Parks have also been classified according to the sizes of the "mid-sized stores" that comprise them. The Thesis proposes a new definition of the Commercial Park, which is more comprehensive and better adapted to the importance of this commercial format, as well as recommendations for design and development based on the urban planning parameters obtained. The Thesis explores the foresseable horizon of Commercial Parks and potential new lines of research.

Análisis y reducción del riesgo de explosión por incendio en los almacenes de pirotecnia

Un incendio en el interior de un almacén de pirotecnia puede provocar la iniciación más o menos simultánea de los artículos existentes en su interior, con su consecuente onda de choque potenciada por la presión de los propios gases generados en su interior. La finalidad de esta tesis doctoral consiste en analizar los fenómenos que se desarrollan como consecuencia de un incendio interior en los almacenes de pirotecnia y establecer posibles medidas eficaces de prevención y protección con objeto de reducir el riesgo de explosión.. Inicialmente se llevó a cabo un análisis del riesgo de explosión, evaluando las consecuencias en caso de producirse un fuego interior. Los resultados fueron catastróficos. Finalmente, y con objeto de minimizar la posibilidad de producirse un incendio en el interior de un almacén, se evaluaron diferentes sistemas de detección y extinción automática de incendios para almacenes pirotécnicos, realizándose pruebas a escala real. En particular, la presente tesis consta de los siguientes trabajos: 1) Evaluación de la sobrepresión generada por la explosión de artificios pirotécnicos en el interior de almacenes, mediante pruebas de explosión de artículos pirotécnicos, con el fin de evaluar las posibles consecuencias en caso de materializarse una explosión, y disponer de información que permita conocer el grado de resistencia de las estructuras de los almacenes, así como el diseño adecuado de las mismas para soportar los efectos de tales explosiones. 2) Evaluación del efecto de un incendio en el interior de un almacén de pirotecnia mediante pruebas a escala real, con el fin de evaluar la posibilidad de la iniciación simultánea de los artículos pirotécnicos almacenados en su interior, y su consecuente explosión, así como las consecuencias en caso de materializarse la explosión. 3) Análisis de sistemas de detección y extinción automática de incendios para almacenes de pirotecnia, mediante la realización de pruebas a escala real para verificar la eficiencia y eficacia de tales sistemas y agentes para sofocar un incendio pirotécnico de forma tal que se evite la posible explosión asociada. 4) Evaluación de nuevos agentes extintores utilizados en los sistemas de extinción automática de incendios, que actualmente se están utilizando en el sector pirotécnico nacional, mediante la realización de pruebas a escala real. Los resultados obtenidos han permitido al Ministerio de Industria, Energía y Turismo actualizar la reglamentación vigente aplicable (Instrucción Técnica Complementaria número 17 del Reglamento de artículos pirotécnicos y cartuchería, aprobado por el Real Decreto 563/2010, de 7 de mayo). ABSTRACT A fire inside a pyrotechnics magazine can cause the more or less simultaneous initiation of the stored articles, with the wave of shock that is produced promoted by the pressure of the gases generated in its interior. The purpose of this PhD thesis is to analyze the phenomena that develop as a result of a fire inside the pyrotechnics stores and establish possible effective prevention and protection measures to reduce the risk of explosionAn analysis of the risk of explosion and fire consequences in a pyrotechnic magazine, testing to real scale, was initially carried out. The results were catastrophic. Finally, and in order to minimize the possibility of a fire inside a pyrotechnic magazine, different systems of detection and automatic extinction of fires in pyrotechnic magazines were evaluated, testing to real scale. Particularly, this thesis has the following parts: 1) Evaluation of the pressure generated by the pyrotechnic explosion in a magazine, with explosión tests of pyrotechnic articles, in order to evaluate the possible consequences in case of materialize an explosion, and provide information to know the resistance grade of the magazine structures, as well as the right design of those to withstand the effects of such explosions. 2) Evaluation of the effect of a fire inside a pyrotechnic magazine by full-scale tests, in order to evaluate the possibility of simultaneous initiation of the pyrotechnic articles stored inside, and the resulting explosion, as well as the consequences in case of materialize an explosion. 3) Analysis and evaluation of detection and automatic fire extinguishing in pyrotechnic magazines, by full-scale tests in order to verify the efficiency and effectiveness of such systems and agents to quell a pyrotechnic fire and avoid the posible mass explosion. 4) Evaluation of new extinguishing agent used in automatic fire extinguishers, which are currently being used in the national fireworks industry, by full-scale tests. The results obtained have allowed the Ministry of Industry, Energy and Tourism, update the current regulation (Complementary Technical Instruction 17 of the Regulation of Pyrotecnic Articles and Ammunition, approved by the Royal Decree 563/2010, of May 7).

Sistema de e-procurement entre un almacén y sus tiendas asociadas con mensajería SOAP

El propósito de este proyecto es el desarrollo de un sistema de aprovisionamiento electrónico para gestionar los pedidos de las tiendas al almacén mediante mensajería SOAP. El sistema consiste en dos aplicaciones Web, la primera instalada en el almacén y otra instalada en las tiendas asociadas a dicho almacén. Ambas aplicaciones se desarrollarán en Java y JSP utilizando el Framework Spring e Hibernate para la persistencia en base de datos. La mensajería entre las aplicaciones se realizará con mensajes SOAP enviados a servicios Web publicados en ambas aplicaciones. En la primera parte del trabajo se realizará una explicación del Framework de Spring e Hibernate focalizando sobre todo en los módulos utilizados en el trabajo. También se realizará una explicación acerca de la mensajería SOAP y los servicios Web. En la segunda parte se realizarán las dos aplicaciones del sistema. La aplicación de gestión de la tienda permitirá a los usuarios realizar pedidos al almacén, recibir las mercancías y consultar el histórico de pedidos realizados. Además tendrá publicados dos servicios web para recibir las expediciones de los pedidos y los productos nuevos o modificados en el almacén. La aplicación de gestión del almacén permitirá a los usuarios crear / modificar productos, expedir los pedidos recibidos de las tiendas y consultar el histórico de pedidos recibidos. Además tendrá publicados dos servicios web para recibir los pedidos y las recepciones de mercancías desde las tiendas. En esta aplicación también se implementará una tarea programada que se ejecutará cada tres minutos y que sincronizará con las tiendas los productos nuevos o modificados en el almacén mediante mensajes SOAP. SUMMARY The aim of this project is the development of an e-procurement system to manage orders from shops to the storehouse using SOAP messaging. The system consists of two Web applications, the first one is installed in the storehouse and the other is installed in the shops associated to that storehouse. Both applications will be developed in Java and JSP using the Spring Framework and Hibernate for database persistence. The messaging between applications is performed with SOAP messages sent to Web services published in both applications. In the first part of the project an explanation of the Spring Framework and Hibernate will be performed, especially focusing on modules used in the project. An explanation about SOAP messaging and Web services will be carried out too. In the second part of the project the two system applications will be performed. The store management application will allow the users to make purchase orders to the storehouse, receive items and consult the order history carried out. In addition it will have two Web Services published in order to receive the shipping orders and the new or modified products in the storehouse. The management application of the storehouse will allow the users to create and modify products, send the orders received from stores and consult the orders history received. Besides, it will have two Web Services published to receive the orders and receipts from stores. A scheduled task run every three minutes will also be performed in this application. It will synchronize the new or modified products with stores using SOAP messaging.

Soluciones constructivas frente al ruido

En el presente proyecto, se han abordado las distintas soluciones constructivas para tratar las principales exigencias en edificación: aislamiento acústico, acondicionamiento acústico y ruido y vibraciones de las instalaciones. Estas soluciones constructivas, se enmarcan dentro de un conjunto de leyes vigentes en España, provenientes de la trasposición de la legislación Europea. Basándose en el Documento Básico HR Protección frente al ruido, donde se encuentran las soluciones constructivas, comentadas anteriormente, se ha llevado a cabo un estudio exhaustivo de las mismas, con la finalidad de crear una herramienta de consulta de fácil y rápido acceso para cualquier persona. En especial, se enfoca a una finalidad didáctica accesible al personal universitario. Se pretende que cualquier persona, con base mínima en la materia, pueda acceder y entender la herramienta creada. Para lograr a esta finalidad, se ha analizado otra documentación, como la Guía de aplicación del DB HR, donde se explica de forma más detallada el Documento Básico HR Protección frente al ruido. Además, se han consultado diferentes normativas españolas, para conseguir que la información aportada al usuario sea completa y no sea necesario acudir a diferentes documentos externos. De esta manera, un usuario podrá realizar un proyecto para: aislar, acondicionar o controlar el ruido y vibraciones de las instalaciones, en una edificación, acudiendo a esta herramienta. Con el objeto de que sea un proyecto enmarcado en un contexto didáctico, con fácil acceso y tratando que sea lo más intuitivo posible, se buscó una plataforma de acceso gratuito y disponible para distintos equipos y sistemas operativos. El programa empleado ha sido tomado del paquete Office creado por Microsoft. El programa se denomina OneNote. Se trata de un programa para realizar, compartir y gestionar notas de forma rápida y sencilla. Los archivos creados en él, se almacenan en OneDrive, por lo tanto, el usuario puede acceder a ellos desde cualquier parte, en cualquier momento y plataforma disponible. ABSTRACT. The aforementioned project approaches the diverse solutions available to accomplish the chief edification requirements in the Spanish Legal System, remarkably influenced by the European Law, in Construction Planning. The key elements of those solutions are acoustic isolation, acoustic conditioning and noise and vibration of installations. Basing most part of our research in the academic report “Documento Básico HR Protección frente al ruido”, which addresses effective solutions about the mentioned queries, but also implements a useful tool that supplies access to the broad public to obtain a wide variety of answers regarding those issues. Its main aim is to improve the knowledge about that subject, however also the access and usage to that system, of every person, although its previous experience. In order to achieve that purpose, the paper also combines an analysis of the study “Guía de aplicación del DB HR” where different elements of the report “Documento Básico HR Protección frente al ruido” are examined in further detail. It is important to remark that all the information provided comply with the current legislation present in Spain at the time this paper was written, so the reader may work on a project of isolation, conditioning or control of noise and vibrations of installations without the requirement of applying external resources. Conclusively, with the intention of giving the paper an educational usage, with intuitive and easy access, the reader is able to join to the platform OneNote by Microsoft Office, software available in utmost part of operating systems and devices. This program allows us to produce, share and manage notes swiftly and efficiently, but also it stores them in The Cloud called OneDrive, so the user can get access to them anywhere and anytime.

As lojas sociais como estruturas de apoio aos indivíduos em situação de vulnerabilidade económica

Dissertação de mestrado na área de Educação Social e Intervenção Comunitária

50 Years Ago We Marched: What began on the grounds of Lincoln University, ended on the steps of the Alabama State Capitol

LINCOLN UNIVERSITY - On March 25, 1965, a bus loaded with Lincoln University students and staff arrived in Montgomery, Ala. to join the Selma march for racial and voting equality. Although the Civil Rights Act of 1964 was in force, African-Americans continued to feel the effects of segregation. The 1960s was a decade of social unrest and change. In the Deep South, specifically Alabama, racial segregation was a cultural norm resistant to change. Governor George Wallace never concealed his personal viewpoints and political stance of the white majority, declaring “Segregation now, segregation tomorrow, segregation forever.” The march was aimed at obtaining African-Americans their constitutionally protected right to vote. However, Alabama’s deep-rooted culture of racial bias began to be challenged by a shift in American attitudes towards equality. Both black and whites wanted to end discrimination by using passive resistance, a movement utilized by Dr. Martin Luther King Jr. That passive resistance was often met with violence, sometimes at the hands of law enforcement and local citizens. The Selma to Montgomery march was a result of a protest for voting equality. The Student Nonviolent Coordinating Committee (SNCC) and the Southern Christian Leadership Counsel (SCLC) among other students marched along the streets to bring awareness to the voter registration campaign, which was organized to end discrimination in voting based on race. Violent acts of police officers and others were some of the everyday challenges protesters were facing. Forty-one participants from Lincoln University arrived in Montgomery to take part in the 1965 march for equality. Students from Lincoln University’s Journalism 383 class spent part of their 2015 spring semester researching the historical event. Here are their stories: Peter Kellogg “We’ve been watching the television, reading about it in the newspapers,” said Peter Kellogg during a February 2015 telephone interview. “Everyone knew the civil rights movement was going on, and it was important that we give him (Robert Newton) some assistance … and Newton said we needed to get involve and do something,” Kellogg, a lecturer in the 1960s at Lincoln University, discussed how the bus trip originated. “That’s why the bus happened,” Kellogg said. “Because of what he (Newton) did - that’s why Lincoln students went and participated.” “People were excited and the people along the sidewalk were supportive,” Kellogg said. However, the mood flipped from excited to scared and feeling intimidated. “It seems though every office building there was a guy in a blue uniform with binoculars standing in the crowd with troops and police. And if looks could kill me, we could have all been dead.” He says the hatred and intimidation was intense. Kellogg, being white, was an immediate target among many white people. He didn’t realize how dangerous the event in Alabama was until he and the others in the bus heard about the death of Viola Liuzzo. The married mother of five from Detroit was shot and killed by members of the Ku Klux Klan while shuttling activists to the Montgomery airport. “We found out about her death on the ride back,” Kellogg recalled. “Because it was a loss of life, and it shows the violence … we could have been exposed to that danger!” After returning to LU, Kellogg’s outlook on life took a dramatic turn. Kellogg noted King’s belief that a person should be willing to die for important causes. “The idea is that life is about something larger and more important than your own immediate gratification, and career success or personal achievements,” Kellogg said. “The civil rights movement … it made me, it made my life more significant because it was about something important.” The civil rights movement influenced Kellogg to change his career path and to become a black history lecturer. Until this day, he has no regrets and believes that his choices made him as a better individual. The bus ride to Alabama, he says, began with the actions of just one student. Robert Newton Robert Newton was the initiator, recruiter and leader of the Lincoln University movement to join Dr. Martin Luther King’s march in Selma. “In the 60s much of the civil rights activists came out of college,” said Newton during a recent phone interview. Many of the events that involved segregation compelled college students to fight for equality. “We had selected boycotts of merchants, when blacks were not allowed to try on clothes,” Newton said. “You could buy clothes at department stores, but no blacks could work at the department stores as sales people. If you bought clothes there you couldn’t try them on, you had to buy them first and take them home and try them on.” Newton said the students risked their lives to be a part of history and influence change. He not only recognized the historic event of his fellow Lincolnites, but also recognized other college students and historical black colleges and universities who played a vital role in history. “You had the S.N.C.C organization, in terms of voting rights and other things, including a lot of participation and working off the bureau,” Newton said. Other schools and places such as UNT, Greenville and Howard University and other historically black schools had groups that came out as leaders. Newton believes that much has changed from 50 years ago. “I think we’ve certainly come a long way from what I’ve seen from the standpoint of growing up outside of Birmingham, Alabama,” Newton said. He believes that college campuses today are more organized in their approach to social causes. “The campus appears to be some more integrated amongst students in terms of organizations and friendships.” Barbara Flint Dr. Barbara Flint grew up in the southern part of Arkansas and came to Lincoln University in 1961. She describes her experience at Lincoln as “being at Lincoln when the world was changing.“ She was an active member of Lincoln’s History Club, which focused on current events and issues and influenced her decision to join the Selma march. “The first idea was to raise some money and then we started talking about ‘why can’t we go?’ I very much wanted to be a living witness in history.” Reflecting on the march and journey to Montgomery, Flint describes it as being filled with tension. “We were very conscious of the fact that once we got on the road past Tennessee we didn’t know what was going to happen,” said Flint during a February 2015 phone interview. “Many of the students had not been beyond Missouri, so they didn’t have that sense of what happens in the South. Having lived there you knew the balance as well as what is likely to happen and what is not likely to happen. As my father use to say, ‘you have to know how to stay on that line of balance.’” Upon arriving in Alabama she remembers the feeling of excitement and relief from everyone on the bus. “We were tired and very happy to be there and we were trying to figure out where we were going to join and get into the march,” Flint said. “There were so many people coming in and then we were also trying to stay together; that was one of the things that really stuck out for me, not just for us but the people who were coming in. You didn’t want to lose sight of the people you came with.” Flint says she was keenly aware of her surroundings. For her, it was more than just marching forward. “I can still hear those helicopters now,” Flint recalled. “Every time the helicopters would come over the sound would make people jump and look up - I think that demonstrated the extent of the tenseness that was there at the time because the helicopters kept coming over every few minutes.” She said that the marchers sang “we are not afraid,” but that fear remained with every step. “Just having been there and being a witness and marching you realize that I’m one of those drops that’s going to make up this flood and with this flood things will move,” said Flint. As a student at Lincoln in 1965, Flint says the Selma experience undoubtedly changed her life. “You can’t expect to do exactly what you came to Lincoln to do,” Flint says. “That march - along with all the other marchers and the action that was taking place - directly changed the paths that I and many other people at Lincoln would take.” She says current students and new generations need to reflect on their personal role in society. “Decide what needs to be done and ask yourself ‘how can I best contribute to it?’” Flint said. She notes technology and social media can be used to reach audiences in ways unavailable to her generation in 1965. “So you don’t always have to wait for someone else to step out there and say ‘let’s march,’ you can express your vision and your views and you have the means to do so (so) others can follow you. Jaci Newsom Jaci Newsom came to Lincoln in 1965 from Atlanta. She came to Lincoln to major in sociology and being in Jefferson City was largely different from what she had grown up with. “To be able to come into a restaurant, sit down and be served a nice meal was eye-opening to me,” said Newsom during a recent interview. She eventually became accustomed to the relaxed attitude of Missouri and was shocked by the situation she encountered on an out-of-town trip. “I took a bus trip from Atlanta to Pensacola and I encountered the worse racism that I have ever seen. I was at bus stop, I went in to be served and they would not serve me. There was a policeman sitting there at the table and he told me that privately owned places could select not to serve you.” Newsom describes her experience of marching in Montgomery as being one with a purpose. “We felt as though we achieved something - we felt a sense of unity,” Newsom said. “We were very excited (because) we were going to hear from Martin Luther King. To actually be in the presence of him and the other civil rights workers there was just such enthusiasm and excitement yet there was also some apprehension of what we might encounter.” Many of the marchers showed their inspiration and determination while pressing forward towards the grounds of the Alabama Capitol building. Newsom recalled that the marchers were singing the lyrics “ain’t gonna let nobody turn me around” and “we shall overcome.” “ I started seeing people just like me,” Newsom said. “I don’t recall any of the scowling, the hitting, the things I would see on TV later. I just saw a sea of humanity marching towards the Capitol. I don’t remember what Martin Luther King said but it was always the same message: keep the faith; we’re going to get where we’re going and let us remember what our purpose is.” Newsom offers advice on what individuals can do to make their society a more productive and peaceful place. “We have come a long way and we have ways to change things that we did not have before,” Newsom said. “You need to work in positive ways to change.” Referencing the recent unrest in Ferguson, Mo., she believes that people become destructive as a way to show and vent anger. Her generation, she says, was raised to react in lawful ways – and believe in hope. “We have faith to do things in a way that was lawful and it makes me sad what people do when they feel without hope, and there is hope,” Newsom says. “Non-violence does work - we need to include everyone to make this world a better place.” Newsom graduated from Lincoln in 1969 and describes her experience at Lincoln as, “I grew up and did more growing at Lincoln than I think I did for the rest of my life.”

COMUNICAÇÃO NO PONTO-DE-VENDA: ASPECTOS SENSORIAIS NA AMBIENTAÇÃO DAS MEGALIVRARIAS

Esta pesquisa tem como objetivo investigar e discutir a ambientação como elemento de comunicação aplicado ao ponto-de-venda livraria e observar e analisar como as atuais lojas se adaptaram a uma realidade de mercado, que visa envolver o cliente em uma experiência prazerosa, tendo em vista a concorrência do comércio virtual e a crescente venda de livros em supermercados. Pretende-se, neste trabalho, compreender a utilização dos diversos elementos sensoriais que envolvem: tato, olfato, paladar, visão e audição no ponto-de-venda físico. O objeto de estudo é a Livraria Cultura do Conjunto Nacional, sua relevância ocorre pelo fato da livraria ser a maior loja em metros quadrados do país. Por tratar-se de um fenômeno contemporâneo inserido no contexto da vida real, a metodologia utilizada é o Estudo de Caso Único e os procedimentos metodológicos são observação direta do consumidor no ponto-de-venda, pesquisa bibliográfica, entrevistas com freqüentadores da loja e com profissionais do setor. Pode-se concluir que as características hedônicas do consumidor contemporâneo que busca experiências prazerosas associada a sinergia da comunicação integrada de marketing favorecem para os resultados positivos apresentados pela livraria.(AU)

COMUNICAÇÃO NO PONTO-DE-VENDA: ASPECTOS SENSORIAIS NA AMBIENTAÇÃO DAS MEGALIVRARIAS

Esta pesquisa tem como objetivo investigar e discutir a ambientação como elemento de comunicação aplicado ao ponto-de-venda livraria e observar e analisar como as atuais lojas se adaptaram a uma realidade de mercado, que visa envolver o cliente em uma experiência prazerosa, tendo em vista a concorrência do comércio virtual e a crescente venda de livros em supermercados. Pretende-se, neste trabalho, compreender a utilização dos diversos elementos sensoriais que envolvem: tato, olfato, paladar, visão e audição no ponto-de-venda físico. O objeto de estudo é a Livraria Cultura do Conjunto Nacional, sua relevância ocorre pelo fato da livraria ser a maior loja em metros quadrados do país. Por tratar-se de um fenômeno contemporâneo inserido no contexto da vida real, a metodologia utilizada é o Estudo de Caso Único e os procedimentos metodológicos são observação direta do consumidor no ponto-de-venda, pesquisa bibliográfica, entrevistas com freqüentadores da loja e com profissionais do setor. Pode-se concluir que as características hedônicas do consumidor contemporâneo que busca experiências prazerosas associada a sinergia da comunicação integrada de marketing favorecem para os resultados positivos apresentados pela livraria.(AU)

Specific association of the gene product of PKD2 with the TRPC1 channel

The function(s) of the genes (PKD1 and PKD2) responsible for the majority of cases of autosomal dominant polycystic kidney disease is unknown. While PKD1 encodes a large integral membrane protein containing several structural motifs found in known proteins involved in cell–cell or cell–matrix interactions, PKD2 has homology to PKD1 and the major subunit of the voltage-activated Ca2+ channels. We now describe sequence homology between PKD2 and various members of the mammalian transient receptor potential channel (TRPC) proteins, thought to be activated by G protein-coupled receptor activation and/or depletion of internal Ca2+ stores. We show that PKD2 can directly associate with TRPC1 but not TRPC3 in transfected cells and in vitro. This association is mediated by two distinct domains in PKD2. One domain involves a minimal region of 73 amino acids in the C-terminal cytoplasmic tail of PKD2 shown previously to constitute an interacting domain with PKD1. However, distinct residues within this region mediate specific interactions with TRPC1 or PKD1. The C-terminal domain is sufficient but not necessary for the PKD2–TRPC1 association. A more N-terminal domain located within transmembrane segments S2 and S5, including a putative pore helical region between S5 and S6, is also responsible for the association. Given the ability of the TRPC to form functional homo- and heteromultimeric complexes, these data provide evidence that PKD2 may be functionally related to TRPC proteins and suggest a possible role of PKD2 in modulating Ca2+ entry in response to G protein-coupled receptor activation and/or store depletion.

Evidence of a role for cyclic ADP-ribose in long-term synaptic depression in hippocampus

Ca2+ released from presynaptic and postsynaptic intracellular stores plays important roles in activity-dependent synaptic plasticity, including long-term depression (LTD) of synaptic strength. At Schaffer collateral–CA1 synapses in the hippocampus, presynaptic ryanodine receptor-gated stores appear to mobilize some of the Ca2+ necessary to induce LTD. Cyclic ADP-ribose (cADPR) has recently been proposed as an endogenous activator of ryanodine receptors in sea urchin eggs and several mammalian cell types. Here, we provide evidence that cADPR-mediated signaling pathways play a key role in inducing LTD. We show that biochemical production of cGMP increases cADPR concentration in hippocampal slices in vitro, and that blockade of cGMP-dependent protein kinase, cADPR receptors, or ryanodine-sensitive Ca2+ stores each prevent the induction of LTD at Schaffer collateral–CA1 synapses. A lack of effect of postsynaptic infusion of either cADPR antagonist indicates a probable presynaptic site of action.

Coordinate regulation of gonadotropin-releasing hormone neuronal firing patterns by cytosolic calcium and store depletion

Elevation of cytosolic free Ca2+ concentration ([Ca2+]i) in excitable cells often acts as a negative feedback signal on firing of action potentials and the associated voltage-gated Ca2+ influx. Increased [Ca2+]i stimulates Ca2+-sensitive K+ channels (IK-Ca), and this, in turn, hyperpolarizes the cell and inhibits Ca2+ influx. However, in some cells expressing IK-Ca the elevation in [Ca2+]i by depletion of intracellular stores facilitates voltage-gated Ca2+ influx. This phenomenon was studied in hypothalamic GT1 neuronal cells during store depletion caused by activation of gonadotropin-releasing hormone (GnRH) receptors and inhibition of endoplasmic reticulum (Ca2+)ATPase with thapsigargin. GnRH induced a rapid spike increase in [Ca2+]i accompanied by transient hyperpolarization, followed by a sustained [Ca2+]i plateau during which the depolarized cells fired with higher frequency. The transient hyperpolarization was caused by the initial spike in [Ca2+]i and was mediated by apamin-sensitive IK-Ca channels, which also were operative during the subsequent depolarization phase. Agonist-induced depolarization and increased firing were independent of [Ca2+]i and were not mediated by inhibition of K+ current, but by facilitation of a voltage-insensitive, Ca2+-conducting inward current. Store depletion by thapsigargin also activated this inward depolarizing current and increased the firing frequency. Thus, the pattern of firing in GT1 neurons is regulated coordinately by apamin-sensitive SK current and store depletion-activated Ca2+ current. This dual control of pacemaker activity facilitates voltage-gated Ca2+ influx at elevated [Ca2+]i levels, but also protects cells from Ca2+ overload. This process may also provide a general mechanism for the integration of voltage-gated Ca2+ influx into receptor-controlled Ca2+ mobilization.

Activation of nicotinic receptor-induced postsynaptic responses to luteinizing hormone-releasing hormone in bullfrog sympathetic ganglia via a Na+-dependent mechanism

Nicotine at very low doses (5–30 nM) induced large amounts of luteinizing hormone-releasing hormone (LHRH) release, which was monitored as slow membrane depolarizations in the ganglionic neurons of bullfrog sympathetic ganglia. A nicotinic antagonist, d-tubocurarine chloride, completely and reversibly blocked the nicotine-induced LHRH release, but it did not block the nerve-firing-evoked LHRH release. Thus, nicotine activated nicotinic acetylcholine receptors and produced LHRH release via a mechanism that is different from the mechanism for evoked release. Moreover, this release was not caused by Ca2+ influx through either the nicotinic receptors or the voltage-gated Ca2+ channels because the release was increased moderately when the extracellular solution was changed into a Ca2+-free solution that also contained Mg2+ (4 mM) and Cd2+ (200 μM). The release did not depend on Ca2+ release from the intraterminal Ca2+ stores either because fura-2 fluorimetry showed extremely low Ca2+ elevation (≈30 nM) in response to nicotine (30 nM). Moreover, nicotine evoked LHRH release when [Ca2+] elevation in the terminals was prevented by loading the terminals with 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid and fura-2. Instead, the nicotine-induced release required extracellular Na+ because substitution of extracellular NaCl with N-methyl-d-glucamine chloride completely blocked the release. The Na+-dependent mechanism was not via Na+ influx through the voltage-gated Na+ channels because the release was not affected by tetrodotoxin (1–50 μM) plus Cd2+ (200 μM). Thus, nicotine at very low concentrations induced LHRH release via a Na+-dependent, Ca2+-independent mechanism.