Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni.

We have constructed plasmids to be used for in vitro signature-tagged mutagenesis (STM) of Campylobacter jejuni and used these to generate STM libraries in three different strains. Statistical analysis of the transposon insertion sites in the C. jejuni NCTC 11168 chromosome and the plasmids of strain 81-176 indicated that their distribution was not uniform. Visual inspection of the distribution suggested that deviation from uniformity was not due to preferential integration of the transposon into a limited number of hot spots but rather that there was a bias towards insertions around the origin. We screened pools of mutants from the STM libraries for their ability to colonize the ceca of 2-week-old chickens harboring a standardized gut flora. We observed high-frequency random loss of colonization proficient mutants. When cohoused birds were individually inoculated with different tagged mutants, random loss of colonization-proficient mutants was similarly observed, as was extensive bird-to-bird transmission of mutants. This indicates that the nature of campylobacter colonization in chickens is complex and dynamic, and we hypothesize that bottlenecks in the colonization process and between-bird transmission account for these observations.

Antiguo Oriente: Cuadernos del Centro de Estudios de Historia del Antiguo Oriente, 2006, n° 4 (número completo)

How old is the Kingdom of Edom? A review of new evidence and recent discussion / Eveline Van Der Steen ; Piotr Bienkowski -- A problem of pedubasts? / Dan´El Kahn -- Le ciel selon l´Hymne Orphique à Ouranos et selon des textes funéraires égyptiens (PT, CT, BD): une brève comparaison préliminaire / Amanda–Alice Marvelia -- An epigraphic reanalysis of Two Stelae from Firs Intermediate Period Dendera in the Cairo Museum / Tracy Musacchio -- Mass production in Mesopotamia / Morris Silver -- Iron Age “negative” pottery: a reassessment / Juan Manuel Tebes -- The Cordage from the 2001- Season of the excavations at Berenike (Egyptian Red Sea Coast): preliminary results / André J. Veldmeijer -- Article review. Carr, David M., Writing of the Tablet of the Heart: origins of scripture and literature / Itamar Singer -- Reseñas bibliográficas -- Política editorial

Una visión argentina de España a principios del siglo XX : los caminos peninsulares de Ernesto Mario Barreda

Número monográfico: El viaje y sus discursos

Las Prophetiae Merlini de Geoffrey de Monmouth en los Baladros castellanos: estado de la cuestión

Resumen: La Historia regum Britanniae (circa 1139) del obispo galés Geoffrey de Monmouth, texto destacado en el corpus latino medieval a causa de sus proyecciones tanto historiográficas como ficcionales, tiene como objeto referir los actos de todos los monarcas célticos de la Gran Bretaña anteriores a la conquista germánica. En este propósito, sus capítulos 112 a 117 obran como una mise en abîme puesto que las Prophetiae Merlini que allí se leen, atribuidas al famoso mago pero inspiradas en la literatura apocalíptica vétero y neotestamentaria, reproducen proféticamente las grandes líneas de contenido de la propia crónica, e incluso anticipan la restauración definitiva de los celtas derrotados. Aunque Wace, Chrétien de Troyes y la tradición romancística francesa posterior, deudora de la Historia en muchos puntos, desecharon el texto a causa de su agudo hermetismo, el mismo sí se difundió por el continente y reapareció asombrosamente interpolado, en versión castellana, dentro de dos tardíos ejemplares del género caballeresco hispánico, el Baladro del sabio Merlín con sus profecías (Burgos, 1498) y la primera parte de la Demanda del santo Grial (Sevilla, 1535). La presente comunicación tiene como objetivos reseñar los actuales conocimientos de la crítica acerca de esta versión castellana de las Prophetiae y señalar los problemas aún pendientes de resolución; serán consideradas las contribuciones fundamentales relativas al original latino y, también, a las versiones francesas conservadas, dada la posibilidad de que nuestra traducción tenga una fuente gala

Altruism with Social Roots: An Emerging Literature

Revised: 2007-01.-- Published as an article in: Revista Desarrollo y Sociedad (2006), Semestre II, pp. 245-260.

A short in-frame deletion in NTRK1 tyrosine kinase domain caused by a novel splice site mutation in a patient with congenital insensitivity to pain with anhidrosis

Background: Congenital insensitivity to pain with anhidrosis (CIPA) is a rare autosomal recessive genetic disease characterized by the lack of reaction to noxious stimuli and anhidrosis. It is caused by mutations in the NTRK1 gene, which encodes the high affinity tyrosine kinase receptor I for Neurotrophic Growth Factor (NGF). -- Case Presentation: We present the case of a female patient diagnosed with CIPA at the age of 8 months. The patient is currently 6 years old and her psychomotor development conforms to her age (RMN, SPECT and psychological study are in the range of normality). PCR amplification of DNA, followed by direct sequencing, was used to investigate the presence of NTRK1 gene mutations. Reverse transcriptase (RT)-PCR amplification of RNA, followed by cloning and sequencing of isolated RT-PCR products was used to characterize the effect of the mutations on NTRK1 mRNA splicing. The clinical diagnosis of CIPA was confirmed by the detection of two splice-site mutations in NTRK1, revealing that the patient was a compound heterozygote at this gene. One of these alterations, c.574+1G > A, is located at the splice donor site of intron 5. We also found a second mutation, c.2206-2 A > G, not previously reported in the literature, which is located at the splice acceptor site of intron 16. Each parent was confirmed to be a carrier for one of the mutations by DNA sequencing analysis. It has been proposed that the c.574+1G > A mutation would cause exon 5 skipping during NTRK1 mRNA splicing. We could confirm this prediction and, more importantly, we provide evidence that the novel c.2206-2A > G mutation also disrupts normal NTRK1 splicing, leading to the use of an alternative splice acceptor site within exon 17. As a consequence, this mutation would result in the production of a mutant NTRK1 protein with a seven aminoacid in-frame deletion in its tyrosine kinase domain. --Conclusions: We present the first description of a CIPA-associated NTRK1 mutation causing a short interstitial deletion in the tyrosine kinase domain of the receptor. The possible phenotypical implications of this mutation are discussed.

Hydrologic aspects of freshening upper Old Tampa Bay, Florida

Upper Old Tampa Bay, a 17-square mile area of Old Tampa Bay, Florida, has been proposed for conversion to a fresh-water lake. The amount of runoff to the proposed lake and its chemical quality are both adequate to freshen and sustain a fresh-water lake in this part of the bay. During 1950-66 runoff to the proposed lake, including discharge from Lake Tarpon, would have averaged 134 mgd (million gallons per day) and would have displaced the volume of the proposed lake at normal pool stage (2.5 feet above mean sea level) about 1.7 times per year. Without discharge from Lake Tarpon, the volume of the proposed lake would have been displaced 1.2 times. If the lake level was initially at a normal pool stage during a critically dry year, such as 1956, the proposed lake would have declined 0.25 to 0.5 foot below the minimum design level, (1.5 feet above mean sea level). (44 page document)

Old Timers Reunion 1928

Leonard Carpenter Panama Canal Collection. Photographs: Views of Panama and the Canal. [Box 1] from the Special Collections & Area Studies Department, George A. Smathers Libraries, University of Florida.

Panama Railroad Station, Balboa, Canal Zone - Old Timers arriving Jan. 18, 1928

Leonard Carpenter Panama Canal Collection. Photographs: Views of Panama and the Canal. [Box 1] from the Special Collections & Area Studies Department, George A. Smathers Libraries, University of Florida.