The proteome of the infectious bronchitis virus Beau-R Virion

Infectious bronchitis is a highly contagious respiratory disease of poultry caused by the coronavirus IBV. It was thought that coronavirus virions were composed of three major viral structural proteins, until investigations of other coronaviruses showed that coronavirus virions also include viral non-structural and group specific proteins as well as host cell proteins. To study the proteome of IBV virions, virus was grown in embryonated chicken eggs and purified by sucrose gradient ultracentrifugation and analysed by mass spectrometry proteomic. Analysis of three preparations of purified IBV yielded the three expected structural proteins plus thirty-five additional virion-associated host proteins. Virion-associated host proteins had a diverse range of functional attributions, being involved in cytoskeleton formation, RNA binding and protein folding pathways. Some of these proteins were unique to this study, whilst others were found to be orthologous to proteins identified in SARS-CoV virions, and also virions from a number of other RNA and DNA viruses. Together these results demonstrate that coronaviruses have the capacity to incorporate a substantial variety of host protein, which may have implications for the disease process.

Sub-tropical urban environment affecting content and composition of non-structural carbohydrates of Lolium multiflorum ssp italicum cv. Lema

This study analyzed the relationship between environmental factors, especially air pollution and climatic conditions, and non-structural carbohydrates (NSC) in plants of Lolium multiflorum exposed during 10 consecutive periods of 28 days at a polluted site (Congonhas) and at a reference site in Sao Paulo city (Brazil). After exposure, NSC composition and leaf concentrations of Al, Fe. Cu, Zn, Pb and Cd were measured. The seasonal pattern of NSC accumulation was quite similar in both sites, but plants at Congonhas showed higher concentrations of these compounds, especially fructans of low and medium degree of polymerization. Regression analysis showed that NSC in plants growing at the polluted site were explained by variations on temperature and leaf concentration of Fe (positive effect), as well as relative humidity and particulate material (negative effect). NSC in the standardized grass culture, in addition to heavy metal accumulation, may indicate stressing conditions in a sub-tropical polluted environment. (C) 2008 Elsevier Ltd. All rights reserved.

Early Phase of Allergic Airway Inflammation in Diabetic Rats: Role of Insulin on the Signaling Pathways and Mediators

Background: Allergic lung inflammation is impaired in diabetic rats and is restored by insulin treatment. In the present study we investigated the effect of insulin on the signaling pathways triggered by allergic inflammation in the lung and the release of selected mediators. Methods: Diabetic male Wistar rats (alloxan, 42 mg/kg, i.v., 10 days) and matching controls were sensitized by s.c. injections of ovalbumin (OA) in aluminium hydroxide, 14 days before OA (1 mg/0.4 ml) or saline intratracheal challenge. A group of diabetic rats were treated with neutral protamine Hagedorn insulin (NPH, 4 IU, s.c.), 2 h before the OA challenge. Six hours after the challenge, bronchoalveolar lavage (BAL) was performed for mediator release and lung tissue was homogenized for Western blotting analysis of signaling pathways. Results: Relative to non-diabetic rats, the diabetic rats exhibited a significant reduction in OA-induced phosphorylation of the extracellular signal-regulated kinase (ERK, 59%), p38 (53%), protein kinase B (Akt, 46%), protein kinase C (PKC)-alpha (63%) and PKC-delta (38%) in lung homogenates following the antigen challenge. Activation of the NF-kappa B p65 subunit and phosphorylation of I kappa B alpha were almost suppressed in diabetic rats. Reduced expression of inducible nitric oxide synthase (iNOS, 32%) and cyclooxygenase-2 (COX-2, 46%) in the lung homogenates was also observed. The BAL concentration of prostaglandin (PG)-E(2), nitric oxide (NO) and interleukin (IL)-6 was reduced in diabetic rats (74%, 44% and 65%, respectively), whereas the cytokine-induced neutrophil chemoattractant (CINC)-2 concentration was not different from the control animals. Treatment of diabetic rats with insulin completely or partially restored all of these parameters. This protocol of insulin treatment only partially reduced the blood glucose levels. Conclusion: The data presented show that insulin regulates MAPK, PI3K, PKC and NF-kappa B pathways, the expression of the inducible enzymes iNOS and COX-2, and the levels of NO, PGE(2) and IL-6 in the early phase of allergic lung inflammation in diabetic rats. It is suggested that insulin is required for optimal transduction of the intracellular signals that follow allergic stimulation. Copyright (C) 2010 S. Karger AG, Basel

Expression and purification of human respiratory syncytial virus recombinant fusion protein

The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The structural quality of the recombinant fusion protein and the estimation of its secondary structure were obtained by circular dichroism. Structural models of the fusion protein presented 46% of the helices in agreement with the spectra by circular dichroism analysis. There are only few studies that succeeded in expressing the HRSV fusion protein in bacteria. This is a report on human fusion protein expression in E. call and structure analysis, representing a step forward in the development of fusion protein F inhibitors and the production of antibodies. (c) 2008 Elsevier Inc. All rights reserved.

Robust tori in a double-waved Hamiltonian model

A Hamiltonian system perturbed by two waves with particular wave numbers can present robust tori, which are barriers created by the vanishing of the perturbed Hamiltonian at some defined positions. When robust tori exist, any trajectory in phase space passing close to them is blocked by emergent invariant curves that prevent the chaotic transport. Our results indicate that the considered particular solution for the two waves Hamiltonian model shows plenty of robust tori blocking radial transport. (C) 2010 Elsevier B.V. All rights reserved.

Plasma confinement in tokamaks with robust torus

We present a non-linear symplectic map that describes the alterations of the magnetic field lines inside the tokamak plasma due to the presence of a robust torus (RT) at the plasma edge. This RT prevents the magnetic field lines from reaching the tokamak wall and reduces, in its vicinity, the islands and invariant curve destruction due to resonant perturbations. The map describes the equilibrium magnetic field lines perturbed by resonances created by ergodic magnetic limiters (EMLs). We present the results obtained for twist and non-twist mappings derived for monotonic and non-monotonic plasma current density radial profiles, respectively. Our results indicate that the RT implementation would decrease the field line transport at the tokamak plasma edge. (C) 2010 Elsevier B.V. All rights reserved.

psi ` to J/psi ratio measurements in PHENIX at RHIC

The ratio of the psi` over the J psi production cross section in the dielectron channel has been measured in root s = 200 GeV p + p collisions with the PHENIX detector at RHIC. The analysis is based on fitting of the dielectron invariant mass spectra in the area around the J psi and psi` signals in order to extract a ratio psi` over J psi of 0.019 +/- 0.005 (stat) +/- 0.002 (sys) and a fractional feed-down contribution to J psi from psi` of 8.6 +/- 2.5%.

Light-Emitting Diode Therapy in Chemotherapy-Induced Mucositis

Background and Objective: Mucositis is the most common oral complication of cancer chemotherapy, which causes pain on mastication and swallowing, impairs patients` ability to eat and take oral drugs and may determine interruption of the treatment. The aim of this study was to evaluate the effect of light-emitting diode (LED) therapy on chemotherapy-induced mucositis in hamsters. Study Design/Materials and Methods: Animals of both experimental (Group 1; n = 32) and positive control (Group II; n = 32) groups received intraperitoneal injections of 5-fluorouracil on days 0 and 2. All animals had their right and left cheek pouch irritated by superficial scratching on days 3 and 4. In Group I, LED irradiation (630 nm +/- 10 nm, 160 mW, 12 J/cm(2)) was applied during 37.5 seconds at days 3, 4, 6, 8, 10, 12, and 14. In Group II, mucositis was induced, but LED therapy was not performed. The oral mucosa was photographed from day 4 to 14 at 2-day intervals. Photographs were randomly scored according to the severity of induced mucositis (0 to 5). In the negative control group (Group III; n = 6), no mucositis was induced. Biopsies of the cheek pouches of 8 animals (Group I and Group II) were surgically obtained on days 5, 9, 13 and 15 and processed for histological examination. Results: The statistical analysis showed significant differences between irradiated and non-irradiated groups (P < 0.05). However, muscular degeneration was observed in 18% of the samples of Group I. Conclusion: It may be concluded that the LED therapy protocol established for this in vivo study was effective in reducing the severity of oral mucositis, although the oral lesions were not completely prevented. Lasers Surg. Med. 40:625-633, 2008. (c) 2008Wiley-Liss, Inc.

A theoretical and mass spectrometry study of the fragmentation of mycosporine-like amino acids

In the present study, the mycosporine-like amino acids (MAAs) were isolated from the marine red alga Gracilaria tenuistipitata and analysed by high-resolution accurate-mass sequential mass spectrometry (MSn). In addition to the proposed fragmentation mechanism based on the MSn analysis, it is clearly demonstrated that the elimination of mass 15 is a radical processes taking place at the methoxyl substituent of the double bond. This characteristic loss of a methyl radical was studied by theoretical calculations and the homolytic cleavage of the O-C bond is suggested to be dependent on the bond weakening. The protonation site of the MAAs was indicated by analysis of the Fukui functions and the relative Gibbs energies of the several possible protonated forms. (C) 2008 Elsevier B.V. All rights reserved.

Biobased composites from tannin-phenolic polymers reinforced with coir fibers

Tannin-phenolic polymers prepared using tannin, a macromolecule obtained from natural sources, were used in the preparation of composites reinforced with coir fibers. The composites based on tannin-phenolic polymers (50% (w/w) of tannin as substitute of the phenol) were prepared using the coir fibers as reinforcement (30-70% (w/w), 3.0-6.0 cm, randomly distributed). The Izod impact strength of the composites showed an improvement in this property due to the incorporation of coir fibers in the tannin-phenolic matrices. The SEM images showed excellent adhesion at the fiber/matrix interface. The coir fiber had bundles regularly spaced, which enhanced the diffusion of the resin into the fiber. In addition, the high lignin content of this fiber results in a high concentration of aromatic rings, which increased the compatibility with the matrix. The values of the diffusion coefficient of water, determined using Fick`s laws, show that there was no correlation between the fiber percentage and the water diffusion. The DMTA curves showed that the storage moduli of the composites reinforced with coir fibers were considerably higher than that of the thermoset, and the increase in the proportion of fibers led to a proportional increase in the storage moduli of these materials. The biobased composites obtained have potential for non-structural applications, such as in the internal parts of automotives vehicles. To our knowledge, this is the first study on this kind of biobased composites. (C) 2010 Elsevier B.V. All rights reserved.

Estudo da viabilidade técnica para a Bolívia de painéis de gesso reforçados com fibra de vidro para paredes internas não portantes

Na construção civil, nos países em desenvolvimento como a Bolívia, continua-se usando técnicas e materiais na execução de paredes, similar a décadas passadas. Os painéis de gesso reforçados com fibra de vidro são uma alternativa de simplificação e agilização na execução das paredes internas não portantes. A presente dissertação objetiva mostrar a viabilidade técnica e econômica dos painéis para paredes internas, na Bolívia; para tanto realizou-se uma avaliação dos recursos disponíveis (gesso), com relação às reservas, produção e custos. A técnica adotada na produção dos painéis é a de pré-mistura, que permite a moldagem dos painéis manual ou mecanicamente com equipamentos simples e de baixo custo. O estudo do compósito de gesso reforçado com fibra de vidro (GRG) foi realizado com gesso brasileiro e com gesso boliviano, verificando-se o seu comportamento mecânico com diferentes teores de fibra de vidro. Verificou-se aumento da resistência a tração na flexão e diminuição da resistência a compressão com o aumento do teor de fibra de vidro. A avaliação do comportamento mecânico dos painéis foi feita através dos ensaios de impacto (corpo mole e corpo duro). Nos ensaios de corpo mole, verificou-se aumento da resistência com o aumento do teor de fibra de vidro. Os teores de 0,75% e 1,5% se mostraram suficientes para que os painéis de GRG satisfaçam os requisitos de desempenho para divisórias internas respectivamente para o gesso brasileiro e boliviano. O comportamento dos painéis nos ensaios de impacto de corpo duro foi satisfatório, tanto para painéis com fibra como para painéis sem fibra de vidro. No estudo preliminar de custos, verificou-se uma redução em torno de 36% para os painéis de GRG em relação às paredes intemas tradicionalmente usadas na Bolívia.

Análise da indústria de televisão a cabo no Brasil : estrutura competitiva e evolução

A indústria de TVa cabo está num caminho convergente com o setor de telecomunicações por força dos avanços tecnológicos relacionados à informática. As redes das operadoras de cabo têm a possibilidade de se transformarem num elemento central na evolução da sociedade da informação. Essa dissertação investiga a estrutura competitiva atual da indústria de TV a cabo brasileira. Para isso, lança mão dos conceitos de Michael E. Porter (1980). Além disso, são descritos possíveis desdobramentos na evolução da estrutura do setor, na visão de especialistas da indústria.

Responsabilidade corporativa: entre o social e o regulado estudo de um setor da siderurgia brasileira

Este trabalho teve como objetivo analisar as dimensões de responsabilidade corporativa "regulada" e a responsabilidade corporativa "social", explorando o conceito de responsabilidade social em relação às práticas de responsabilidade social e regulada, bem como sua aplicação no ramo siderúrgico brasileiro, especificamente nas seguintes empresas: Companhia Siderúrgica Nacional, CSN e Companhia Siderúrgica Paulista, COSIP A. Em linhas gerais, os resultados chamaram a atenção para o fato de que, em grande parte, o que se vem chamando de responsabilidade social, não passa do cumprimento às determinações legais. Dessa forma, entender a responsabilidade que as empresas têm para o desenvolvimento de uma sociedade requer verificar em que medida as organizações estão contribuindo para um desenvolvimento econômico e social, que leve em conta noções de sustentabilidade e que vão além das determinações pautadas pela legislação vigente. No tratamento dos dados coletados nas entrevistas utilizou-se análise do discurso como uma forma de entender as relações intertextuais e interdiscursivas, ou seja, compreender as disfuncionalidades entre o que se diz e o que se faz. Além disso, serviram de suporte para análise pesquisas documental e bibliográfica do tema em questão.

Modelagem de previsões econômicas em cenários prospectivos

A estabilidade econômica que o Brasil atualmente experimenta permite resgatar a tradição de construção e análises prospectivas de médio e longo prazos. Isto remete à necessidade de se pensar mais profundamente acerca da possível evolução da economia brasileira, sendo mister o conhecimento de ferramentas de previsão. O objetivo deste trabalho é contribuir com este propósito. Avanços significativos têm sido observados na modelagem de previsões macro econômicas. Para se avaliar como os modelos de previsão estarão se desenvolvendo no futuro próximo, é necessário entender a evolução das abordagens dos modelos de previsão não-estruturais e estruturais. Como dependem da teoria econômica, os modelos estruturais ganham importância e descaso na medida em que a teoria evolui e cai em desuso. A marca registrada das previsões macroeconômicas nos próximos anos será o casamento das melhores abordagens estruturais e não-estruturais, facilitadas por avanços em técnicas numéricas e de simulação. Para ilustrar o trabalho de forma empírica, três cenários para a economia brasileira foram desenvolvidos, utilizando-se como base o arcabouço teórico resumido em uma das seções deste trabalho. Cenários potencialmente têm um valor muito grande na gestão de uma empresa ou de uma instituição. Cenários são a oitava ferramenta gerencial mais utilizada nas empresas em todo o mundo1 e esta dissertação é mais uma contribuição para este rico campo.

Essays on structural transformation, growth and education

This thesis aims to study the impact of structural change on the trajectory of development of emerging economies. More speci cally, we seek to understand how the reallocation of labor from less productive sectors of the economy (e.g., agriculture) to more productive sectors (e.g., industry and services) contributed to the growth of labor productivity in these economies. The thesis is divided into three chapters, besides the introduction. The rst chapter studies the relationship between structural change and economic development in Latin American economies. While the process of reallocation of labor was important to the dynamics of productivity in the period of convergence of these economies, low productivity in some sectors of the economy explained most of the reduction in productivity in the most recent period. In the second chapter, I study the main determinants of growth of the Chinese economy between 1980 and 2005. I show that the increased ow of trade and strong productivity growth in the agricultural sector contributed signi -cantly to China s development in the period. In the third chapter, I study the apparent contradiction between increased levels of schooling and reduction of per capita income in African economies compared to the U.S. economy. The main conclusion is that reducing educational costs explain the retreat of the education di¤erential between African economies and the United States.