Molecular confirmation of ovine herpesvirus 2-induced malignant catarrhal fever lesions in cattle from Rio Grande do Norte, Brazil

Molecular findings that confirmed the participation of ovine herpesvirus 2 (OVH-2) in the lesions that were consistent with those observed in malignant catarrhal fever of cattle are described. Three mixed-breed cattle from Rio Grande do Norte state demonstrated clinical manifestations that included mucopurulent nasal discharge, corneal opacity and motor incoordination. Routine necropsy examination demonstrated ulcerations and hemorrhage of the oral cavity, corneal opacity, and lymph node enlargement. Significant histopathological findings included widespread necrotizing vasculitis, non-suppurative meningoencephalitis, lymphocytic interstitial nephritis and hepatitis, and thrombosis. PCR assay performed on DNA extracted from kidney and mesenteric lymph node of one animal amplified a product of 423 base pairs corresponding to a target sequence within the ovine herpesvirus 2 (OVH-2) tegument protein gene. Direct sequencing of the PCR products, from extracted DNA of the kidney and mesenteric lymph node of one cow, amplified the partial nucleotide sequences (423 base pairs) of OVH-2 tegument protein gene. Blast analysis confirmed that these sequences have 98-100% identity with similar OVH-2 sequences deposited in GenBank. Phylogenetic analyses, based on the deduced amino acid sequences, demonstrated that the strain of OVH-2 circulating in ruminants from the Brazilian states of Rio Grande do Norte and Minas Gerais are similar to that identified in other geographical locations. These findings confirmed the active participation of OVH-2 in the classical manifestations of sheep associated malignant catarrhal fever.

Ocupaçâo, valorizaçâo e comércio de terras no norte do Rio Grande do Sul - séculos 19 e 20

This article focuses on some aspects of the agrarian history of Soledade, a town in the north of the state of Rio Grande do Sul, southern Brazil. Our aim is to understand how this area was in many ways affected by the Land Law of 1850, particularly in economic and social terms. Population growth, arrival of European immigrants and increasing privatization of the commons contributed for the increasing of land prices in the region during the second half of the 19 th century. Understanding this situation as a great opportunity to make money, some landowners, directly or through a colonization company, allotted and sold the land they had previously acquired through the Land Law of 1850

Ocupaçâo, valorizaçâo e comércio de terras no norte do Rio Grande do Sul - séculos 19 e 20

This article focuses on some aspects of the agrarian history of Soledade, a town in the north of the state of Rio Grande do Sul, southern Brazil. Our aim is to understand how this area was in many ways affected by the Land Law of 1850, particularly in economic and social terms. Population growth, arrival of European immigrants and increasing privatization of the commons contributed for the increasing of land prices in the region during the second half of the 19 th century. Understanding this situation as a great opportunity to make money, some landowners, directly or through a colonization company, allotted and sold the land they had previously acquired through the Land Law of 1850

Ocupaçâo, valorizaçâo e comércio de terras no norte do Rio Grande do Sul - séculos 19 e 20

This article focuses on some aspects of the agrarian history of Soledade, a town in the north of the state of Rio Grande do Sul, southern Brazil. Our aim is to understand how this area was in many ways affected by the Land Law of 1850, particularly in economic and social terms. Population growth, arrival of European immigrants and increasing privatization of the commons contributed for the increasing of land prices in the region during the second half of the 19 th century. Understanding this situation as a great opportunity to make money, some landowners, directly or through a colonization company, allotted and sold the land they had previously acquired through the Land Law of 1850

Limites constitucionais do decreto regulamentar na criação e extinção de órgãos e cargos públicos no âmbito do Estado do Rio Grande do Norte / Vladimir da Rocha França. --

Inclui bibliografia.