Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae

A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (German Bight) were examined: Laminaria saccharina (Phaeophyta), Chondrus crispus (Rhodophyta), and Ulva lactuca (Chlorophyta). In L. saccharina and C. crispus, the adenylate energy charge (AEC) was determined in different thallus regions. AEC varied in relation to tissue age and function. Higher AEC values typically occurred in thallus regions with meristematic activity. Furthermore, L. saccharina and U. lactuca were exposed to UV-A and elevated UV-B radiation. The AEC was calculated and the maximal quantum yield of photosystem II (Fv/Fm) was determined as indicators for UV stress. In both species, the AEC remained at high values (0.72 ± 0.04), while Fv/Fm dropped rapidly. The results show that the photosynthesis of the phaeophyte is more resistant to UV radiation than the chlorophyte.

3He/4He ratios of pore gases in pore water and chemical composition of sediments at DSDP Sites 87-583 and 87-584

We have measured the 3He/4He and 20Ne/4He ratios of gases dissolved in the pore water in sediments at two sites in the Nankai Trough (Site 583) and the Japan Trench (Site 584). The 3He/4He and 20Ne/4He ratios vary from 0.215 * 10**-6 to 1.23 * 10**-6 and from 50 * 10**-3 to 2700 * 10**-3, respectively. These values can be explained by mixing two components, one from the atmosphere and one with a 3He/4He ratio of (0.2 to 0.3) * 10**-6. The latter component may be derived from the ocean crust near the subduction zone.

(Table 1) Deuterium ratios, chloride content and hydrate volume of pore water and hydrate meltwater from ODP Site 164-996

Site 996 is located above the Blake Diapir where numerous indications of vertical fluid migration and the presence of hydrate existed prior to Ocean Drilling Program (ODP) Leg 164. Direct sampling of hydrates and visual observations of hydrate-filled veins that could be traced 30-40 cm along cores suggest a connection between fluid migration and hydrate formation. The composition of pore water squeezed from sediment cores showed large variations due to melting of hydrate during core recovery and influence of saline water from the evaporitic diapir below. Analysis of water released during hydrate decomposition experiments showed that the recovered hydrates contained significant amounts of pore water. Solutions of the transport equations for deuterium (d2H) and chloride (Cl-) were used to determine maximum (d2H) and minimum (Cl-) in situ concentrations of these species. Minimum in situ concentrations of hydrate were estimated by combining these results with Cl- and d2H values measured on hydrate meltwaters and pore waters obtained by squeezing of sediments, by the means of a method based on analysis of distances in the two-dimensional Cl- d2H space. The computed Cl- and d2H distribution indicates that the minimum hydrate amount solutions are representative of the actual hydrate amount. The highest and mean hydrate concentrations estimates from our model are 31% and 10% of the pore space, respectively. These concentrations agree well with visual core observations, supporting the validity of the model assumptions. The minimum in situ Cl- concentrations were used to constrain the rates of upward fluid migration. Simulation of all available data gave a mean flow rate of 0.35 m/k.y. (range: 0.125-0.5 m/k.y.).

Movable-bed laboratory experiments comparing radiation stress and energy flux factor as predictors of longshore transport rate /

"April 1981."

Surfactant effects on pesticide photochemistry in water and soil /

Grant no. R-802959.

Compendium of ERT surface water and sediment sampling procedures : interim final.

Mode of access: Internet.

Sorption properties of sediments and energy-related pollutants /

Mode of access: Internet.

Handbook for sampling and sample preservation of water and wastewater.

Mode of access: Internet.

Management of the Tongass National Forest [microform] : hearings before the Subcommittee on Forests, Family Farms, and Energy of the Committee on Agriculture, House of Representatives, One Hundredth Congress, second session, on H.R. 1516, May 25 and 26, 1988.

"Serial no. 100-86."

H.R. 4226--Renewable Energy and Energy Conservation Commercialization and Development Act [microform] : hearing before the Subcommittee on Energy Research and Development of the Committee on Science, Space, and Technology, House of Representatives, One Hundredth Congress, second session, June 30, 1988.

"No. 126."

Timber sales on national forests [microform] : hearings before the Subcommittee on Forests, Family Farms, and Energy of the Committee on Agriculture, House of Representatives, One Hundredth Congress, first session, June 17 and 18, 1987.

"Serial no. 100-47."

Exchange certain national forest system lands in the Targhee National Forest [microform] : hearing before the Subcommittee on Forests, Family Farms, and Energy of the Committee on Agriculture, House of Representatives, One Hundredth Congress, second session, on H.R. 4028, April 16, 1988; Driggs, ID and April 27, 1988.

"Serial no. 100-88."

Middle Atlantic Interstate Forest Fire Protection Compact to include the state of Ohio; land conveyance in Henderson, TN; and release a revisionary interest of the United States in certain land located in Oktibbeha County, MS [microform] : hearings before the Subcommittee on Forests, Family Farms, and Energy of the Committee on Agriculture, House of Representatives, One Hundredth Congress, second session, on H.R. 2756, H.R. 2835, and H.R. 4724, May 4, 1988 ... September 22, 1988 ....

"Serial no. 100-89."

Colonia Water and Sewage Service Act [microform] : hearing before the Subcommittee on Conservation, Credit, and Rural Development of the Committee on Agriculture, House of Representatives, One Hundredth Congress, second session, on H.R.3524, September 22, 1988.

"Serial no. 100-114."

Review of the drug law enforcement activities of the Forest Service; and the Omnibus Public Lands Act of 1987 [microform] : hearing before the Subcommittee on Forests, Family Farms, and Energy of the Committee on Agriculture, House of Representatives, One Hundredth Congress, second session, on H.R. 3680, July 12, 1988.

"Serial no. 100-92."