Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae


Autoria(s): Hünken, Mark; Karsten, Ulf; Wiencke, Christian
Data(s)

16/07/2005

Resumo

A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (German Bight) were examined: Laminaria saccharina (Phaeophyta), Chondrus crispus (Rhodophyta), and Ulva lactuca (Chlorophyta). In L. saccharina and C. crispus, the adenylate energy charge (AEC) was determined in different thallus regions. AEC varied in relation to tissue age and function. Higher AEC values typically occurred in thallus regions with meristematic activity. Furthermore, L. saccharina and U. lactuca were exposed to UV-A and elevated UV-B radiation. The AEC was calculated and the maximal quantum yield of photosystem II (Fv/Fm) was determined as indicators for UV stress. In both species, the AEC remained at high values (0.72 ± 0.04), while Fv/Fm dropped rapidly. The results show that the photosynthesis of the phaeophyte is more resistant to UV radiation than the chlorophyte.

Formato

application/zip, 2 datasets

Identificador

https://doi.pangaea.de/10.1594/PANGAEA.848162

doi:10.1594/PANGAEA.848162

Idioma(s)

en

Publicador

PANGAEA

Direitos

CC-BY: Creative Commons Attribution 3.0 Unported

Access constraints: unrestricted

Fonte

Supplement to: Hünken, Mark; Karsten, Ulf; Wiencke, Christian (2005): Determination of the adenylate energy charge (AEC) as a tool to determine the physiological status of macroalgal tissues after UV exposure. Phycologia, 44(3), 249-253, doi:10.2216/0031-8884(2005)44[249:DOTAEC]2.0.CO;2

Palavras-Chave #Adenosine 5-Triphosphate; Adenosine 5-Triphosphate, standard deviation; Adenosine diphosphate; Adenosine diphosphate, standard deviation; Adenosine monophosphate; Adenosine monophosphate, standard deviation; Adenylate energy charge ratio; Adenylate energy charge ratio, standard deviation; Adenylates; Adenylates, total; Adenylates, total, standard deviation; Adenylates std dev; ADP; ADP std dev; AEC ratio; AEC ratio std dev; AMP; AMP std dev; ATP; ATP std dev; DFG-Schwerpunktprogramm 1158 - Antarktisforschung; DFG-SPP1158; N; Sample amount; Sample type; Samp type; Species; thallus region; Time; Time in hours; Treatm; Treatment
Tipo

Dataset