Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites

Fluorescent proteins have proven to be important tools for in vitro live imaging of parasites and for imaging of parasites within the living host by intravital microscopy. We observed that a red fluorescent transgenic malaria parasite of rodents, Plasmodium berghei-RedStar, is suitable for in vitro live imaging experiments but bleaches rapidly upon illumination in intravital imaging experiments using mice. We have therefore generated two additional transgenic parasite lines expressing the novel red fluorescent proteins tdTomato and mCherry, which have been reported to be much more photostable than first- and second-generation red fluorescent proteins including RedStar. We have compared all three red fluorescent parasite lines for their use in in vitro live and intravital imaging of P. berghei blood and liver parasite stages, using both confocal and wide-field microscopy. While tdTomato bleached almost as rapidly as RedStar, mCherry showed improved photostability and was bright in all experiments performed.

Results of a joint U.S.A./U.S.S.R. hydrodynamic and transport modeling project /

"February 1979."

Development and verification of a three-dimensional numerical hydrodynamic, salinity, and temperature model of Chesapeake Bay.

Authors: B.H. Johnson, R.E. Heath, B.B. Hsieh, K.W. Kim, H.L. Butler.

User's guide for a three-dimensional numerical hydrodynamic, salinity, and temperature model of Chesapeake Bay /

Authors: Billy H. Johnson, Ronald E. Heath, Bernard B. Hsieh, Keu W. Kim, H. Lee Butler.

Lower Green Bay hydrodynamic and mass transport : numerical model study /

"December 1987."

Imidazole and its derivatives /

Includes bibliographies and index.

Asymptotic estimation of errors and derivatives for the numerical solution of ordinary differential equations,

"Supported in part by contract US AEC AT(11-1)2383."

A study of the efficiency of Illiac IV in hydrodynamic calculations /

Includes bibliographies.

Hydrodynamic damping and "added mass" for flexible offshore platforms /

"October 1976."

Review of three classic problems in hydrodynamic stability.

"Supported by Office of Naval Research [under Contract N6-ONR-27135]"

Dictionary of organic compounds; the constitution and physical and chemical properties of the principal carbon compounds and their derivatives, together with the relevant literature references.

Accompanied by "Supplement ... [to vol. 2-3]" (2 v.) Published: New York, Oxford University Press, 1943.

Dictionary of organic compounds; the constitution and physical and chemical properties of the principal carbon compounds and their derivatives, together with the relevant literature references.

Errata slips inserted.

A list of Christian names, their derivatives, nicknames and equivalents in several foreign languages.

Mode of access: Internet.

An Anglo-Saxon grammar, and derivatives;

Mode of access: Internet.