Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites


Autoria(s): Graewe, Stefanie; Retzlaff, Silke; Struck, Nicole; Janse, Chris J; Heussler, Volker
Data(s)

01/06/2009

Resumo

Fluorescent proteins have proven to be important tools for in vitro live imaging of parasites and for imaging of parasites within the living host by intravital microscopy. We observed that a red fluorescent transgenic malaria parasite of rodents, Plasmodium berghei-RedStar, is suitable for in vitro live imaging experiments but bleaches rapidly upon illumination in intravital imaging experiments using mice. We have therefore generated two additional transgenic parasite lines expressing the novel red fluorescent proteins tdTomato and mCherry, which have been reported to be much more photostable than first- and second-generation red fluorescent proteins including RedStar. We have compared all three red fluorescent parasite lines for their use in in vitro live and intravital imaging of P. berghei blood and liver parasite stages, using both confocal and wide-field microscopy. While tdTomato bleached almost as rapidly as RedStar, mCherry showed improved photostability and was bright in all experiments performed.

Formato

application/pdf

Identificador

http://boris.unibe.ch/84086/1/895_ftp.pdf

Graewe, Stefanie; Retzlaff, Silke; Struck, Nicole; Janse, Chris J; Heussler, Volker (2009). Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites. Biotechnology journal, 4(6), pp. 895-902. Wiley 10.1002/biot.200900035 <http://dx.doi.org/10.1002/biot.200900035>

doi:10.7892/boris.84086

info:doi:10.1002/biot.200900035

info:pmid:19492329

urn:issn:1860-6768

Idioma(s)

eng

Publicador

Wiley

Relação

http://boris.unibe.ch/84086/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Graewe, Stefanie; Retzlaff, Silke; Struck, Nicole; Janse, Chris J; Heussler, Volker (2009). Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites. Biotechnology journal, 4(6), pp. 895-902. Wiley 10.1002/biot.200900035 <http://dx.doi.org/10.1002/biot.200900035>

Palavras-Chave #570 Life sciences; biology
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed