Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong

Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.

Bacterial roles in the formation of high-molecular-weight dissolved organic matter in estuarine and coastal waters: Evidence from lipids and the compound-specific isotopic ratios

High-molecular-weight dissolved organic matter (HMW-DOM, > 1,000 Daltons) is actively involved in the global biogeochemical cycling of many elements, but its carbon sources and detailed formation pathways are still not well understood. In this study, we measured bulk stable carbon and nitrogen isotopic ratios, lipid composition, and compound-specific carbon isotopic ratios of HMW-DOM samples collected from four U.S. estuaries (Boston Harbor/Massachusetts Bay, Delaware/Chesapeake Bay, San Diego Bay, and San Francisco Bay). Analytical results show (1) a fraction of HMW-DOM (lipid associated) in estuarine and coastal waters is derived from bacteria and phytoplankton; (2) this fraction of HMW-DOM is formed by various release processes of bacterial membrane components and bacterial reworking of phytoplankton-derived material; (3) this fraction of HMW-DOM is generally present in all samples from different coastal systems despite variable organic matter inputs and environmental conditions, suggesting an important bacterial role in HMW-DOM formation.

A produção de vinhos em regiões tropicais: anais.

Características dos solos agrícolas da região semi-árida do Vale do São Francisco. Bases de dados georreferenciadas para a seleção de áreas vitícolas do Vale do Submédio São Francisco . O clima vitícola do Submédio São Francisco e o zoneamento dos períodos de produção de uvas para elaboração de vinhos. Variabilidade espacial do clima no Vale do Submédio São Francisco com vistas ao zoneamento mesoclimático . Zonificación de terroir aplicado a zonas sin tradición vitícola: el caso del Valle Submedio del Río San Francisco (Pernambuco, Brasil). Comportamiento fisiológico de la vid en climas cálidos y en particular durante el período de maduración de la uva. Técnicas de produção vitícola com ciclos sucessivos em condições tropicais. Análise dos atuais sistemas de produção de uvas para vinho no Vale do São Francisco. Introdução e avaliação de novas cultivares para vinho no Vale do São Francisco. Manejo de água na cultura da videira. Problemas fitossanitários na viticultura do Vale do São Francisco . Problemas entomológicos na viticultura do Vale do São Francisco. Selección de cultivares y desarrollo de nuevos viños de calidad en climas cálidos: comportamiento agronómico y enológico en la región de jerez, España. Estabilidade da matéria corante dos vinhos de regiões de clima quente. Peculiaridades de la vinificación de uvas producidas en climas cálidos. Diferenciação da composição fenólica de vinhos portugueses induzida por colagens protéicas. Características das uvas do Vale do São Francisco sob o ponto de vista enológico. Características analíticas e sensoriais de vinhos produzidos no Vale do Submédio São Francisco, Brasil. Desafios e prioridades para o desenvolvimento científico e tecnológico da produção de vinhos de qualidade em zonas tropicais.

Impact of Early Postnatal Androgen Exposure on Voice Development

Background: the impact of early postnatal androgen exposure on female laryngeal tissue may depend on certain characteristics of this exposure. We assessed the impact of the dose, duration, and timing of early androgen exposure on the vocal development of female subjects who had been treated for adrenocortical tumor (ACT) in childhood.Methods: the long-term effects of androgen exposure on the fundamental vocal frequency (F0), vocal pitch, and final height and the presence of virilizing signs were examined in 9 adult (age, 18.4 to 33.5 years) and 10 adolescent (13.6 to 17.8 years) female ACT patients. We also compared the current values with values obtained 0.9 years to 7.4 years after these subjects had undergone ACT surgery, a period during which they had shown normal androgen levels.Results: of the 19 subjects, 17 (89%) had been diagnosed with ACT before 4 years of age, 1 (5%) at 8.16 years, and 1 (5%) at 10.75 years. Androgen exposure (2 to 30 months) was sufficiently strong to cause pubic hair growth in all subjects and clitoromegaly in 74% (14/19) of the subjects, but did not reduce their height from the target value. Although androgen exposure induced a remarkable reduction in F0 (132 Hz) and moderate pitch virilization in 1 subject and partial F0 virilization, resulting in F0 of 165 and 169 Hz, in 2 subjects, the majority had normal F0 ranging from 189 to 245 Hz.Conclusions: Female laryngeal tissue is less sensitive to androgen exposure between birth and adrenarche than during other periods. Differential larynx sensitivity to androgen exposure in childhood and F0 irreversibility in adulthood are age-, concentration-, duration-, and timing-dependent events that may also be affected by exposure to inhibitory or stimulatory hormones. Further studies are required to better characterize each of these factors.

Teaching by Example in Food Assembly by Robot

T. G. Williams, J.J. Rowland, Lee M.H. and M.J. Neal Teaching by Example in Food Assembly by Robot, Proc. 2000 IEEE Int. Conf. On Robotics and Automation, San Francisco, April 2000, pp3247-52.

Interpreting Analytical Spectra with Evolutionary Computation

Rowland, J.J. (2002) Interpreting Analytical Spectra with Evolutionary Computation. In: Fogel, G.B. and Corne, D.W. (eds), Evolutionary Computation in Bioinformatics. Morgan Kaufmann, San Francisco, pp 341--365, ISBN 1-55860-797-8

Teatro historico de las Iglesias del Reyno de Aragon : tomo primero preliminar [-IX]

En Tomo V. 2 h. de grabados calcográficos representando monedas de Huesca.

Diagnóstico de materias primas vegetales para la producción artesanal en el Alto Putumayo, Valle de Sibundoy.

38 hojas : ilustraciones, fotografías.

Cuaderno de diseño de las asesorías de tejeduría en lana y carpintería en madera Valle de Sibundoy - Putumayo : etapa de producción : carpintería en chonta y guadua Mocoa - Putumayo : Etapa de desarrollo de producto.

91 hojas.

Informe de avance de asesoría en diseño familias vinculadas al programa de desarrollo alternativo-PDA.

40 hojas : ilustraciones, fotografías.

Diagnóstico de materias primas vegetales para la producción artesanal en el Alto Putumato, Valle de Sibundoy.

39 hojas : ilustraciones, fotografías.