Nanoflagellates (mixotrophs, heterotrophs and autotrophs) in the oligotrophic eastern Mediterranean

The vertical distribution (0 to 100 m) and abundance of nanoflagellates were examined in the oligotrophic Aegean Sea (east Mediterranean) in early spring (south basin) and late summer (north and south basins) of 1997 in the framework of the MATER project (Mass Transfer and Ecosystem Response). Different trophic types of nanoflagellates (mixotrophic, heterotrophic, and phototrophic) were identified based on the possession of chloroplasts and the consumption of Fluorescently Labelled Minicells (FLM). Bacterial production (leucine method) was compared with bacterivory estimated from FLM consumption. We found that mixotrophic nanoflagellates played a small role as bacterivores relative to heterotrophic nanoflagellates and total bacterivory roughly balanced bacterial production. In early spring with cool (14.2°C) well-mixed water columns, flagellate concentrations were lowest, phototrophic flagellates were the dominant group and concentrations varied little with depth. Average concentrations of mixotrophs, heterotrophs and autotrophs were 0.07, 0.34, and 0.64 x 103 cells/ml, respectively. Bacterial production in the 0 to 100 m layer averaged about 0.74 µg C/l/d. Estimated nanoflagellate bacterivory from FLM ingestion accounted for 40% of bacterial production with mixotrophic nanoflagellates consuming 5% of bacterial production. In late summer, total nanoflagellate concentrations were higher. Average concentrations of mixotrophs, heterotrophs and autotrophs were 0.09, 1.14, and 0.66 x 103 cells/ml, respectively, in the southern basin and 0.09, 1.1, and 0.98 x 103 cells/ml, respectively, in the northern basin. In September, bacterial production for both basins roughly balanced estimated nanoflagellate consumption. Similar to the March estimates, mixotrophic nanoflagellates accounted for about 5% of nanoflagellate bacterivory. In a nutrient enrichment experiment in March, treatments including phosphorus resulted in increased bacterial production and reductions in identifiable mixotrophs.

Carbon sources in the North Sea evaluated by means of carbon isotope tracers

A multitracer approach is applied to assess the impact of boundary fluxes (e.g., benthic input from sedi- ments or lateral inputs from the coastline) on the acid-base buffering capacity, and overall biogeochemistry, of the North Sea. Analyses of both basin-wide observations in the North Sea and transects through tidal basins at the North-Frisian coastline, reveal that surface distributions of the d13C signature of dissolved inorganic carbon (DIC) are predominantly controlled by a balance between biological production and respiration. In particular, variability in metabolic DIC throughout stations in the well-mixed southern North Sea indi- cates the presence of an external carbon source, which is traced to the European continental coastline using naturally occurring radium isotopes (224Ra and 228Ra). 228Ra is also shown to be a highly effective tracer of North Sea total alkalinity (AT) compared to the more conventional use of salinity. Coastal inputs of meta- bolic DIC and AT are calculated on a basin-wide scale, and ratios of these inputs suggest denitrification as a primary metabolic pathway for their formation. The AT input paralleling the metabolic DIC release prevents a significant decline in pH as compared to aerobic (i.e., unbuffered) release of metabolic DIC. Finally, long- term pH trends mimic those of riverine nitrate loading, highlighting the importance of coastal AT production via denitrification in regulating pH in the southern North Sea.

Diurnal fluctuations in seawater pH influence the response of a calcifying macroalga to ocean acidification

Coastal ecosystems that are characterized by kelp forests encounter daily pH fluctuations, driven by photosynthesis and respiration, which are larger than pH changes owing to ocean acidification (OA) projected for surface ocean waters by 2100. We investigated whether mimicry of biologically mediated diurnal shifts in pH-based for the first time on pH time-series measurements within a kelp forest-would offset or amplify the negative effects of OA on calcifiers. In a 40-day laboratory experiment, the calcifying coralline macroalga, Arthrocardia corymbosa, was exposed to two mean pH treatments (8.05 or 7.65). For each mean, two experimental pH manipulations were applied. In one treatment, pH was held constant. In the second treatment, pH was manipulated around the mean (as a step-function), 0.4 pH units higher during daylight and 0.4 units lower during darkness to approximate diurnal fluctuations in a kelp forest. In all cases, growth rates were lower at a reduced mean pH, and fluctuations in pH acted additively to further reduce growth. Photosynthesis, recruitment and elemental composition did not change with pH, but ?(13)C increased at lower mean pH. Including environmental heterogeneity in experimental design will assist with a more accurate assessment of the responses of calcifiers to OA.

Distribution of organic-walled dinoflagellate cysts in surface sediments of the Benguela upwelling system

To obtain insight in the relationship between the spatial distribution of organic-walled dinoflagellate cysts (dinocysts) and local environmental conditions, fifty-eight surface sediment samples from the coastal shelf off SW Africa were investigated on their dinocyst content with special focus on the two main river systems and the active upwelling that characterise this region. To avoid possible overprint by species-selective preservation, samples have been selected mainly from shelf sites where high sedimentation rates and/or low bottom water oxygen concentrations prevail. Multivariate ordination analyses have been carried out to investigate the relationship between the distribution patterns of individual species to environmental parameters of the upper water column and sediment transport processes. The main oceanographical variables at the surface (temperature, salinity, nutrients chlorophyll-a) in the region show onshore-offshore gradients. This pattern is reflected in the dinocyst associations with high relative abundances of heterotrophic dinocyst species in neritic regions characterised by high chlorophyll-aand low salinity conditions in surface waters. Phototrophic dinocyst species, notably Operculodinium centrocarpum, dominate in the more oceanic area. Differences in the distribution of phototrophic dinocyst species can be related to sea surface salinity and sea surface temperature gradients and to a lesser extent to chlorophyll-a concentrations. Apart from longitudinal gradients the dinocyst distribution clearly reflects regional environmental features. Six groups of species can be distinguished, characteristic for (1) coastal regions (cysts of Polykrikos kofoidii and Selenopemphix quanta), (2) the vicinity of active upwelling (Brigantedinium spp., Echinidinium aculeatum, Echinidinium spp. and Echinidinium transparantum), (3) river mouths (Lejeunecysta oliva, cysts of Protoperidinium americanum, Selenopemphix nephroides and Votadinium calvum), (4) slope and open ocean sediments (Dalella chathamense, Impagidinium patulum and Operculodinium centrocarpum, (5) the southern Benguela region (south of 24°S) (Spiniferites ramosus) and (6) the northern Benguela region (north of 24°S) (Nematosphaeropsis labyrinthus and Pyxidinopsis reticulata). No indication of overprint of the palaeo-ecological signal by lateral transport of allochthonous species could be observed.

Response of the calcifying coccolithophore Emiliania huxleyi to low pH/high pCO2: from physiology to molecular level, 2011

The emergence of ocean acidification as a significant threat to calcifying organisms in marine ecosystems creates a pressing need to understand the physiological and molecular mechanisms by which calcification is affected by environmental parameters. We report here, for the first time, changes in gene expression induced by variations in pH/pCO2 in the widespread and abundant coccolithophore Emiliania huxleyi. Batch cultures were subjected to increased partial pressure of CO2 (pCO2; i.e. decreased pH), and the changes in expression of four functional gene classes directly or indirectly related to calcification were investigated. Increased pCO2 did not affect the calcification rate and only carbonic anhydrase transcripts exhibited a significant down-regulation. Our observation that elevated pCO2 induces only limited changes in the transcription of several transporters of calcium and bicarbonate gives new significant elements to understand cellular mechanisms underlying the early response of E. huxleyi to CO2-driven ocean acidification.

Seawater carbonate chemistry, fertilization and early development of the coral eating crown-of-thorns starfish, Acanthaster planci in a laboratory experiment

Outbreaks of crown-of-thorns starfish (COTS), Acanthaster planci, contribute to major declines of coral reef ecosystems throughout the Indo-Pacific. As the oceans warm and decrease in pH due to increased anthropogenic CO2 production, coral reefs are also susceptible to bleaching, disease and reduced calcification. The impacts of ocean acidification and warming may be exacerbated by COTS predation, but it is not known how this major predator will fare in a changing ocean. Because larval success is a key driver of population outbreaks, we investigated the sensitivities of larval A. planci to increased temperature (2-4 °C above ambient) and acidification (0.3-0.5 pH units below ambient) in flow-through cross-factorial experiments (3 temperature × 3 pH/pCO2 levels). There was no effect of increased temperature or acidification on fertilization or very early development. Larvae reared in the optimal temperature (28 °C) were the largest across all pH treatments. Development to advanced larva was negatively affected by the high temperature treatment (30 °C) and by both experimental pH levels (pH 7.6, 7.8). Thus, planktonic life stages of A. planci may be negatively impacted by near-future global change. Increased temperature and reduced pH had an additive negative effect on reducing larval size. The 30 °C treatment exceeded larval tolerance regardless of pH. As 30 °C sea surface temperatures may become the norm in low latitude tropical regions, poleward migration of A. planci may be expected as they follow optimal isotherms. In the absence of acclimation or adaptation, declines in low latitude populations may occur. Poleward migration will be facilitated by strong western boundary currents, with possible negative flow-on effects on high latitude coral reefs. The contrasting responses of the larvae of A. planci and those of its coral prey to ocean acidification and warming are considered in context with potential future change in tropical reef ecosystems.

Calcium carbonate vein compositions from drill sites in the Atlantic and Pacific

Chemical (Sr, Mg) and isotopic (d18O, 87Sr/86Sr) compositions of calcium carbonate veins (CCV) in the oceanic basement were determined to reconstruct changes in Sr/Ca and Mg/Ca of seawater in the Cenozoic. We examined CCV from ten basement drill sites in the Atlantic and Pacific, ranging in age between 165 and 2.3 Ma. Six of these sites are from cold ridge flanks in basement <46 Ma, which provide direct information about seawater composition. CCV of these young sites were dated, using the Sr isotopic evolution of seawater. For the other sites, temperature-corrections were applied to correct for seawater-basement exchange processes. The combined data show that a period of constant/low Sr/Ca (4.46 - 6.22 mmol/mol) and Mg/Ca (1.12 - 2.03 mol/mol) between 165 and 30 Ma was followed by a steady increase in Mg/Ca ratios by a factor of three to modern ocean composition. Mg/Ca - Sr/Ca relations suggest that variations in hydrothermal fluxes and riverine input are likely causes driving the seawater compositional changes. However, additional forcing may be involved in explaining the timing and magnitude of changes. A plausible scenario is intensified carbonate production due to increased alkalinity input to the oceans from silicate weathering, which in turn is a result of subduction-zone recycling of CO2 from pelagic carbonate formed after the Cretaceous slow-down in ocean crust production rate.

Boundaries of firms and catching up by latecomers in global production networks : the case of a Mexican auto-parts manufacturer

For manufacturing firms in developing countries, there are high barriers to entry and to catching up with competitors in their global production networks (GPNs). This paper examines the case of a Mexican auto-parts manufacturer that succeeded in catching up in the automotive GPN. The author proposes that the door to GPNs is open thanks to frequent changes in the boundaries of firms, and also stresses the importance of the necessary conditions that generate opportunities, including institutional settings that facilitate market entry and catching up, and capability building by firms hopeful of entry.

Cadena de valor de papas nativas (Solanum andigenum sp.) en la provincia de Jauja, Perú

La Asamblea General de la ONU, a solicitud del gobierno peruano, declara en el año 2008 el Año Internacional de la Papa, (AIP). Desde el año 2005, el gobierno peruano ha puesto en marcha estrategias en torno a la importancia de la papa, como la declaración del 30 de Mayo como el Día Nacional de la Papa. El año 2014 es declarado por la FAO, (Food and Agriculture Organization of the United Nations), Año Internacional de la Agricultura Familiar, con un enfoque compartido por esta Tesis Doctoral en el apoyo a la familia campesina peruana. El gobierno peruano utiliza las cadenas de valor como una estrategia de promoción de desarrollo sostenible, que ha permitido contribuir a la inclusión social y económica de productores pobres de zonas alto andinas, como las localizadas en la mancomunidad municipal del Yacus, (MMY), provincia de Jauja, departamento de Junín. Esta estrategia, en la que el mercado incorpora a los pequeños agricultores de las zonas altas de los Andes, (que disponen de recursos económicos muy bajos), en procesos productivos rentables, implica una serie de cambios a realizar, como la transformación de los patrones de producción tradicional hacia aquellos productos o servicios que tienen demanda en el mercado, o la variación de la mentalidad del agricultor pequeño hacia una concepción empresarial de su producción. (Fabián, 2013). Por otra parte, la sostenibilidad de las cadenas de valor depende del eslabón más débil, lo que obliga a conocer la situación de todos los eslabones para poder integrar y reforzar la cadena. Se requiere un sistema de transparencia adecuado que facilite el flujo de la información entre los distintos eslabones. (Briz et al., 2012). Además, el establecimiento de la cadena de valor debe hacerse con cuidado, ya que la eficacia y supervivencia de las empresas están cada vez más ligadas a la cadena de valor a la que pertenece y a la coordinación de la misma. (Briz, 2011). En esta situación, adquiere importancia el estudio de la cadena de valor de la papa nativa, para una vez establecidas sus características, poder determinar la viabilidad o no de una cadena de valor de este producto que repercuta parte de la riqueza generada en los pequeños agricultores alto andinos, e incluso, de su extensión hasta España. Existen estudios sobre las papas nativas, realizadas por diferentes universidades de distintos países, e incluso, de diferentes continentes. Sin embargo, la mayoría de los estudios se centran en la mejora de la producción de la papa nativa. La novedad de la investigación realizada en la presente Tesis Doctoral radica en el estudio de la viabilidad de la comercialización de la papa nativa, mediante el establecimiento de una cadena de valor que se inicie en la provincia de Jauja, Perú, y finalice tanto en los mercados peruanos de las regiones de Junín y Lima, como en España. El objetivo planteado en esta investigación es la mejora de las condiciones económicas y sociales de las comunidades agrícolas de la provincia de Jauja en el Perú, así como fomentar su desarrollo tecnológico e industrial, mediante el fomento de la cadena de valor de la papa nativa y sus derivados. Se establecen como objetivos específicos la caracterización de los eslabones de la cadena de valor de la papa nativa y sus derivados en la provincia de Jauja del Perú y en España, de manera que se determine el valor agregado en los mismos; el fortalecimiento de las organizaciones de productores de papas nativas para la comercialización de sus producciones y para el fomento de la cultura empresarial; y el desarrollo de una cadena de comercialización papas nativas y sus derivados con origen en la Provincia de Jauja, Perú, y que finalice en España, con la venta al consumidor español. Para alcanzar estos objetivos la metodología utilizada es la cadena de valor agroalimentaria, utilizando como herramientas de análisis el análisis DAFO de la cadena de valor de la papa nativa. Las fuentes de información primarias utilizadas proceden en parte del proyecto de cooperación de UPM, “Mejora de la cadena de valor de la patata andina como impulso al desarrollo rural. Caso de tres Comunidades Campesinas en la Provincia de Jauja del Perú”, en el que participó el doctorando, y en parte proceden de la batería de encuestas específicamente diseñadas para los diferentes eslabones de la cadena de valor de la papa nativa. Las fuentes de información secundarias proceden de artículos académicos publicados, de artículos publicados por revistas especializadas del sector y de informes realizados por diferentes instituciones gubernamentales, tanto españolas como peruanas. Las conclusiones de la investigación son las siguientes. La creación de la mancomunidad del Yacus ha beneficiado a los pequeños agricultores. Estos consiguen mejores condiciones de venta y mejores precios para sus productos, lo que repercute en la mejora de sus condiciones de vida. Estas mejoras en las condiciones de venta de los productos se deben a su pertenencia a una cadena de valor de papa nativa que está funcionando de forma eficaz. Las empresas consideradas para constituir la cadena de valor han mostrado interés por formar parte de ella: los campesinos para obtener mejores precios por sus productos y unas mejores condiciones de venta; los distribuidores para asegurarse una calidad determinada de unas variedades fijas de papa nativa; la industria transformadora por disponer de un suministro de producto adecuado al derivado de papa nativa correspondiente, (hojuelas, tunta, etc.); las empresas exportadoras para tener suministro garantizado de los productos que ellos requieren en los volúmenes adecuados. Es una situación ventajosa para todas las empresas participantes. A pesar de trabajar con un producto tradicional, la cadena de valor de la papa nativa presenta innovación en los productos comercializados, tanto en la papa nativa fresca como en sus derivados, en los formatos de los productos, en la red de distribución, en las instituciones peruanas y en el consumidor final. Se percibe una demanda de papa nativa y de sus productos derivados en aquellos países donde existen comunidades de latinoamericanos que han emigrado de sus países de origen. España está entre los países que han acogido a un importante número de personas de origen latinoamericano. A pesar de la fuerte crisis económica sufrida por España, que ha llevado consigo la vuelta a sus países de origen de parte de su comunidad latinoamericana, el tamaño de esta población sigue siendo importante. Esta población demanda productos originarios de sus propios países, y los consumirían de forma frecuente si los precios son adecuados a su capacidad de consumo. El precio de venta de la papa nativa y sus derivados en España es de gran importancia. La importación de estos productos desde Perú hace que este eleve a niveles que le resta competitividad, en especial en la papa fresca. Se aconseja la búsqueda de empresas que puedan adaptar la producción de la papa fresca de forma local, y mantener para los derivados la exportación directa a España. Las preferencias de los consumidores peruanos y españoles en cuanto a formatos y marcas se refieren no son coincidentes. De las encuestas realizadas, se concluye que no puede seguirse la misma estrategia de marketing en ambos países, debiéndose diferenciar los formatos de los paquetes de la papa nativa y de sus derivados en España y en Perú, para así lograr llegar a los consumidores potenciales de ambos países. ABSTRACT At the request of the Peruvian government, the UN General Assembly declared the International Year of the Potato in 2008. Since 2005, the Peruvian government has implemented strategies around the importance of the potato, as the declaration of the 30th of May as the National Day of the Potato. FAO (Food and Agriculture Organization of the United Nations) has declared 2014 as the International Year of Family Farming, with an approach shared by this Ph.D. dissertation about the Peruvian peasant family. The Peruvian government uses value chains as a strategy to promote sustainable development, which has allowed to contribute to the social and economic inclusion of poor farmers in the high Andean regions as those located in the municipal commonwealth of Yacus (MMY) province of Jauja, department of Junín. This strategy, which incorporates small farmers in the high Andean regions, (who have very low income), to the market with profitable production processes, implies a number of changes that should take place, such as changing patterns of traditional production to those products or services that are in demand in the market, or changes in the mentality of the small farmer into a concept of production business. (Fabián, 2013). Moreover, the sustainability of value chains depends on the weakest link, which demands a knowledge of the status of all the links, in order to integrate and strengthen the chain. It is required an adequate transparency to facilitate the flow of information between the various actors. (Briz et al., 2012). Furthermore, the establishment of the value chain should be done carefully, since the effectiveness and the survival of the businesses are increasingly linked to the value chain where the firm is included and to its coordination. (Briz, 2011). In this situation, it becomes important to study the value chain of the native potato, once we establish its features, to be able to determine the feasibility or not of a value chain of this product, which has an impact of the generated wealth in small farms of the high Andean regions, and even the extension of this value chain to Spain. There are studies on native potatoes, made by different universities in several countries and even in more than one continent. However, most studies focus on improving the production of native potato. The originality of the research conducted in this Ph.D. dissertation is the study of the feasibility of commercialization of native potato, by the creation of a value chain that starts in the province of Jauja, Perú, and ends both in Peruvian markets in the region of Lima, and in Spain. The main goal of this research is to improve the economic and social conditions of farming communities in the province of Jauja in Perú, while promoting its technological and industrial development, by the establishment of a value chain of the native potato and derivatives. The specific objectives of the research are the characterization of the links in the value chain of the native potato and its derivatives in the province of Jauja, (Perú) and in Spain, in order to determine the added value; the strengthening of organizations of native potato producers, to commercialize their products and the promotion of enterprise culture; and the development of a chain to market native potato and its derivatives, with its origin in the province of Jauja, (Perú), and its end in Spain, with the sale to the Spanish consumer. In order to achieve these objectives, the used methodology is the agrifood value chain, using as a tool to analysis it the SWOT analysis of the value chain of the native potato. The primary sources of information used in the research come partly from UPM cooperation project, "Improving the value chain of Andean potato as a boost to rural development. Case Three Rural Communities in the Province of Jauja, (Perú)", in which the Ph.D. student was involved, and partly from the surveys, which were specifically designed for the different links of the value chain of the native potato. The secondary sources of information come from academic articles, from articles published by magazines of the industry, and from reports of several government institutions, both Spanish and Peruvian. The conclusions of the research are as follows. The creation of the commonwealth of Yacus has benefited small farmers. They get better sales conditions and better prices for their products, which results in the improvement of their living conditions. These improvements are due to a value chain of native potato which is working effectively. All the firms invited to constitute the value chain have shown interest in being part of it: the farmers to get better prices for their products and better sale conditions; the distributors to ensure a certain quality of fixed varieties of native potato, the processing industry in order to have an adequate supply of product to the corresponding derivative of native potato (chips, “tunta”, etc.); exporting firms to have a guaranteed supply of the products that they require with the right volumes. It's a win-win situation for all participating companies. Despite being a traditional product, the value chain of the native potato presents innovation in marketed products, (both fresh native potato and its derivatives), in the formats of products, in the distribution network, in Peruvian institutions and in relation with the consumer. There is a perceived demand of native potato and its products in countries where communities of Latin Americans have settled down. Spain is among the countries that have received a significant number of people from Latin America. Despite the strong economic crisis suffered by Spain, which has lead to a return to their home countries of part of the Latin American community, the size of this population is still considerable. This population demands products from their own countries, and they frequently consume them if the prices are suitable to their standard of living. The selling price of the native potato and its derivatives in Spain is of great importance. The import of these products from Perú makes the prices rise to levels that reduce competitiveness, especially in fresh native potatoes. It is advised to look for companies which can adapt the fresh potato production in our country, and keep direct export to Spain for the derivatives products. The preferences of Peruvian and Spanish consumers in terms of formats and brands are not the same. The surveys concluded that the same marketing strategy cannot be followed in both countries. Packet formats of native potato and its derivatives should be differentiated in Spain and Perú, in order to reach the potential consumers of both countries.

Can seed production and restricted dispersal limit recruitment in Pinus pinaster Aiton from the Spanish Northern Plateau?

Natural regeneration faces increasing difficulties in dry forests from the Mediterranean basin, including for normally well-regenerating species such as maritime pine (Pinus pinaster Aiton). In this paper, we studied female fertility, seed dispersal and spread rates in P. pinaster from the Spanish Northern Plateau, where natural regeneration failure is a main concern for forest managers. For this purpose we periodically collected data from seed traps and trees located at two core locations across several years. We found significant variation in interannual cone production, with the best seed trees being the same across years. In addition, we found highly skewed distributions of female reproductive effort and large fertility differences across stands located few kilometres away. Annual seed dispersal kernels fitted lognormal or 2Dt models depending on the stand analysed, with median dispersal distances between 14 and 25 m. Kernels fitted for maximum dispersal periods showed an outstanding intraseasonal variation of median dispersal distances, from 10 to 54 m, in association to variable patterns of rainfall and maximum wind speed. The amount of seed produced appeared to be enough to guarantee the natural regeneration of the stands during the typical 20-year regeneration period. Colonisation simulations concluded that Mediterranean maritime pine has a notable dispersion capacity, which is strongly influenced by levels of fecundity and, especially, by the number and frequency of long-distance dispersal events. The latter play a key role in tree dispersion processes through enlarging the occupied area and fostering the invasion of abandoned crop land.

Toxicity of ZnO nanoparticles, ZnO bulk and ZnCl2 on earthworms in a spiked natural soil and toxicological effects of leachates on aquatic organisms

The present study assessed the uptake and toxicity of ZnO nanoparticles (NPs), ZnO bulk, and ZnCl2 salt in earthworms in spiked agricultural soils. In addition, the toxicity of aqueous extracts to Daphnia magna and Chlorella vulgaris was analyzed to determine the risk of these soils to the aquatic compartment. We then investigated the distribution of Zn in soil fractions to interpret the nature of toxicity. Neither mortality nor differences in earthworm body weight were observed compared with the control. The most sensitive end point was reproduction. ZnCl2 was notably toxic in eliminating the production of cocoons. The effects induced by ZnO-NPs and bulk ZnO on fecundity were similar and lower than those of the salt. In contrast to ZnO bulk, ZnO-NPs adversely affected fertility. The internal concentrations of Zn in earthworms in the NP group were greater than those in the salt and bulk groups, although bioconcentration factors were consistently <1. No relationship was found between toxicity and internal Zn amounts in earthworms. The results from the sequential extraction of soil showed that ZnCl2 displayed the highest availability compared with both ZnO. Zn distribution was consistent with the greatest toxicity showed by the salt but not with Zn body concentrations. The soil extracts from both ZnO-NPs and bulk ZnO did not show effects on aquatic organisms (Daphnia and algae) after short-term exposure. However, ZnCl2 extracts (total and 0.45-μm filtered) were toxic to Daphnia.

Visual attention and perception models for assessing quality in 2D and 3D stereoscopic video

La medida de calidad de vídeo sigue siendo necesaria para definir los criterios que caracterizan una señal que cumpla los requisitos de visionado impuestos por el usuario. Las nuevas tecnologías, como el vídeo 3D estereoscópico o formatos más allá de la alta definición, imponen nuevos criterios que deben ser analizadas para obtener la mayor satisfacción posible del usuario. Entre los problemas detectados durante el desarrollo de esta tesis doctoral se han determinado fenómenos que afectan a distintas fases de la cadena de producción audiovisual y tipo de contenido variado. En primer lugar, el proceso de generación de contenidos debe encontrarse controlado mediante parámetros que eviten que se produzca el disconfort visual y, consecuentemente, fatiga visual, especialmente en lo relativo a contenidos de 3D estereoscópico, tanto de animación como de acción real. Por otro lado, la medida de calidad relativa a la fase de compresión de vídeo emplea métricas que en ocasiones no se encuentran adaptadas a la percepción del usuario. El empleo de modelos psicovisuales y diagramas de atención visual permitirían ponderar las áreas de la imagen de manera que se preste mayor importancia a los píxeles que el usuario enfocará con mayor probabilidad. Estos dos bloques se relacionan a través de la definición del término saliencia. Saliencia es la capacidad del sistema visual para caracterizar una imagen visualizada ponderando las áreas que más atractivas resultan al ojo humano. La saliencia en generación de contenidos estereoscópicos se refiere principalmente a la profundidad simulada mediante la ilusión óptica, medida en términos de distancia del objeto virtual al ojo humano. Sin embargo, en vídeo bidimensional, la saliencia no se basa en la profundidad, sino en otros elementos adicionales, como el movimiento, el nivel de detalle, la posición de los píxeles o la aparición de caras, que serán los factores básicos que compondrán el modelo de atención visual desarrollado. Con el objetivo de detectar las características de una secuencia de vídeo estereoscópico que, con mayor probabilidad, pueden generar disconfort visual, se consultó la extensa literatura relativa a este tema y se realizaron unas pruebas subjetivas preliminares con usuarios. De esta forma, se llegó a la conclusión de que se producía disconfort en los casos en que se producía un cambio abrupto en la distribución de profundidades simuladas de la imagen, aparte de otras degradaciones como la denominada “violación de ventana”. A través de nuevas pruebas subjetivas centradas en analizar estos efectos con diferentes distribuciones de profundidades, se trataron de concretar los parámetros que definían esta imagen. Los resultados de las pruebas demuestran que los cambios abruptos en imágenes se producen en entornos con movimientos y disparidades negativas elevadas que producen interferencias en los procesos de acomodación y vergencia del ojo humano, así como una necesidad en el aumento de los tiempos de enfoque del cristalino. En la mejora de las métricas de calidad a través de modelos que se adaptan al sistema visual humano, se realizaron también pruebas subjetivas que ayudaron a determinar la importancia de cada uno de los factores a la hora de enmascarar una determinada degradación. Los resultados demuestran una ligera mejora en los resultados obtenidos al aplicar máscaras de ponderación y atención visual, los cuales aproximan los parámetros de calidad objetiva a la respuesta del ojo humano. ABSTRACT Video quality assessment is still a necessary tool for defining the criteria to characterize a signal with the viewing requirements imposed by the final user. New technologies, such as 3D stereoscopic video and formats of HD and beyond HD oblige to develop new analysis of video features for obtaining the highest user’s satisfaction. Among the problems detected during the process of this doctoral thesis, it has been determined that some phenomena affect to different phases in the audiovisual production chain, apart from the type of content. On first instance, the generation of contents process should be enough controlled through parameters that avoid the occurrence of visual discomfort in observer’s eye, and consequently, visual fatigue. It is especially necessary controlling sequences of stereoscopic 3D, with both animation and live-action contents. On the other hand, video quality assessment, related to compression processes, should be improved because some objective metrics are adapted to user’s perception. The use of psychovisual models and visual attention diagrams allow the weighting of image regions of interest, giving more importance to the areas which the user will focus most probably. These two work fields are related together through the definition of the term saliency. Saliency is the capacity of human visual system for characterizing an image, highlighting the areas which result more attractive to the human eye. Saliency in generation of 3DTV contents refers mainly to the simulated depth of the optic illusion, i.e. the distance from the virtual object to the human eye. On the other hand, saliency is not based on virtual depth, but on other features, such as motion, level of detail, position of pixels in the frame or face detection, which are the basic features that are part of the developed visual attention model, as demonstrated with tests. Extensive literature involving visual comfort assessment was looked up, and the development of new preliminary subjective assessment with users was performed, in order to detect the features that increase the probability of discomfort to occur. With this methodology, the conclusions drawn confirmed that one common source of visual discomfort was when an abrupt change of disparity happened in video transitions, apart from other degradations, such as window violation. New quality assessment was performed to quantify the distribution of disparities over different sequences. The results confirmed that abrupt changes in negative parallax environment produce accommodation-vergence mismatches derived from the increasing time for human crystalline to focus the virtual objects. On the other side, for developing metrics that adapt to human visual system, additional subjective tests were developed to determine the importance of each factor, which masks a concrete distortion. Results demonstrated slight improvement after applying visual attention to objective metrics. This process of weighing pixels approximates the quality results to human eye’s response.