Complete protection against P. berghei malaria upon heterologous prime/boost immunization against circumsporozoite protein employing Salmonella type III secretion system and Bordetella adenylate cyclase toxoid

Sterile immunity against malaria can be achieved by the induction of IFNgamma-producing CD8(+) T cells that target infected hepatocytes presenting epitopes of the circumsporozoite protein (CSP). In the present study we evaluate the protective efficacy of a heterologous prime/boost immunization protocol based on the delivery of the CD8(+) epitope of Plasmodium berghei CSP into the MHC class I presentation pathway, by either a type III secretion system of live recombinant Salmonella and/or by direct translocation of a recombinant Bordetella adenylate cyclase toxoid fusion (ACT-CSP) into the cytosol of professional antigen-presenting cells (APCs). A single intraperitoneal application of the recombinant ACT-CSP toxoid, as well as a single oral immunization with the Salmonella vaccine, induced a specific CD8(+) T cell response, which however conferred only a partial protection on mice against a subsequent sporozoite challenge. In contrast, a heterologous prime/boost vaccination with the live Salmonella followed by ACT-CSP led to a significant enhancement of the CSP-specific T cell response and induced complete protection in all vaccinated mice.

Application of active and passive suspension techniques to improve high speed ground vehicle performance - phase II. Final report.

Transportation Department, Office of University Research, Washington, D.C.

Evaluation of passive belts for different size occupants. Final report supplement.

National Highway Traffic Safety Administration, Washington, D.C.

Immunization of adults : a call to action.

Mode of access: Internet.

Public attitudes toward passive restraint systems. Summary report.

National Highway Traffic Safety Administration, Washington, D.C.

An Evaluation handbook for health education programs in immunization /

Mode of access: Internet.

The effects of restraining boundaries on the passive resistance of sand; results of a series of tests with a medium-scale testing apparatus. Report submitted to the Office of Naval Research, Dept. of the Navy,

"Project No. Nr-081-117. Contract No. Nonr-100 9(00)"

Public attitudes toward passive restraints. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Enhancements to passive warning devices at railroad-highway grade crossings.

Texas Department of Transportation, Austin

Railroad grade crossing passive signing study. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.

A comparative study of four passive building energy simulations : DOE-2.1, BLAST SUNCAT-2.4, DEROB-III /

"Work Performed Under Contract No. EG-77-C-01-4042."

International passive architectural survey ; announcement of results /

"Prepared Under Task No. 6324.40."

State Department : serious problems in the anthrax vaccine immunization program : report to congressional requesters /

"GAO-01-21."