Análisis de los cambios epigenéticos en la metilación del DNA inducidos por la diabetes y sus posibles mecanismos

Los cambios epigenéticos son responsables de la aparición de muchas patologías humanas y sus causas son debido a factores ambientales como genéticos. Se ha descrito en enfermedades crónicas como la Diabetes Mellitus tipo 2 (T2DM) que se caracteriza por los estados de hiperglucemia y el incremento en el estrés oxidativo que conlleva a complicaciones micro y macro vasculares, asociado a una desmetilación global del genoma. Nuestra hipótesis corresponde a que los órganos diana son afectados por las alteraciones como la metilación e hidroximetilación como consecuencia del estrés oxidativo que luego repercuten en la persistencia de la enfermedad. Métodos: A partir de sangre periférica se analizaron los cambios globales en la metilación del DNA que son afectados por el estado metabólico de 60 individuos (40 pacientes, 20 controles sanos). Por técnicas de cuantificación se compararon los resultados obtenidos con los de la expresión de las enzimas involucradas. Por último, se realizó un estudio de microarreglos de metilación del DNA y de expresión obtenidos de la base de datos GEO para así comparar los resultados con nuestros datos experimentales. Resultados: Los pacientes diabéticos con pobre control metabólico presentaron mayores niveles de metilación que el grupo control y no se encontró alteración en las enzimas involucradas en este proceso. Los resultados fueron concordantes con el estudio de microarreglos. Conclusión: Los estudios experimentales y de microarreglos demostraron que la metilación es tejido específico y que existe una mayor oxidación en pacientes. Por ello proponemos una vía alterna de desmetilación no enzimática, basada en la oxidación directa de los grupos metilos generados por los estados oxidativos característicos de esta enfermedad.

Alternativas de integración de ecosistemas estratégicos a la planificación urbana. Estudio de caso: el crecimiento urbano sobre la función eco-sistémica del corredor natural Usaquén – Suba. Periodo 2003– 2016

Los modelos de gestión urbana a nivel global, incorporan en la planeación y en el desarrollo de los entornos urbanos, al suelo natural de soporte como estructura fundamental; debido principalmente a sus funciones ecosistémicas y a los bienes ambientales que provee, incluyendo la biodiversidad y la conservación de entornos con calidad paisajística, entre otros. Teniendo en cuenta lo anterior, el objetivo principal de esta investigación, consiste en hacer un seguimiento y caracterización del ecosistema estratégico del corredor biológico borde norte en la ciudad de Bogotá, con el fin de identificar y dar visibilidad a los aspectos críticos, que son necesarios a tener en cuenta en la política e instrumentos de planeación, para el tratamiento de ésta área. En el desarrollo de esta investigación, se aplicaron las nociones metodológicas de la ecología urbana, dando inicio con una fase de diagnóstico y caracterización de la situación actual, seguido de la formulación de alternativas, entre las que se destacan, la restauración ecológica. En el diagnóstico se pudo identificar que, si bien existe disponibilidad de agua y de cobertura vegetal en las condiciones de suelo, también está presente una gran presión inmobiliaria en el sector, lo cual, ha promovido la alteración de los predios, haciendo urgente un tratamiento integral de restauración ecológica de éste corredor, que permita la recuperación de la función ecosistémica, aportando beneficios a la ciudad.

OntoSPARES: da linguagem natural às ontologias. Contributos para a classificação automática de dados históricos (séc. XVI-XVIII)

O processamento de linguagem natural e as ontologias são ferramentas cuja interação permite uma melhor compreensão dos dados armazenados. Este trabalho, ao associar estas duas áreas aos elementos disponíveis numa base de dados prosopográfica, tornou possível identificar e classificar relacionamentos entre setores de ocupação na forma como eram designados na época, setores de atividade num formato mais próximo do de hoje e o estatuto social que essas incumbências tinham na sociedade coeva. Os dados utilizados são sobretudo de membros do Santo Ofício – do século XVI ao século XVIII. Para atingir este objetivo utilizaram-se algumas descrições textuais de ocorrências da época e outras pouco estruturadas, disponíveis no repositório SPARES. A aplicação de processamento de linguagem natural (remoção de stopwords e aplicação de stemming), conjugada com a construção de duas ontologias, tornou possível classificar esses dados, permitindo consultas mais eficazes. Ao contribuir para a classificação automática de dados históricos, propõem-se metodologias que podem ser aplicadas em dados de qualquer outra área do conhecimento, especialmente as que lidam com as variáveis de tempo e espaço de forma mais intensa; Abstract: OntoSPARES: from natural language to ontologies Contributions to the automatic classification of historical data (16th-18th centuries) The interaction between the natural language processing and ontologies are tools allowing a better understanding of the data stored. This work, by combining these two areas to the elements available in a prosopographic database, has made possible to identify and classify relationships between occupations of many individuals (in general Holy Office members of the 16th-18th centuries). To achieve this goal the data used was gathered in SPARES repository, including some textual descriptions of the time occurrences. They are all few structured. The application of natural language processing (stopwords removal and stemming application), combined with the construction of two ontologies, made possible to classify those data, allowing a more effective search. By contributing to the automatic classification of historical data, this thesis proposes methodologies that can be applied to data from any other field of knowledge, specially data dealing with time and space variables.

Urban public policies: towards the improvement of landscape ecological system. The case-study of the city of Évora, Portugal.

The Charter of European Planning 2013 presents a Vision for the future of European cities and regions, highlighting the sustainability of cities and the preservation of urban ecosystems, integrating the man-made environment with the natural ecosystems and contribute to the well-being and quality of life of their inhabitants and other stakeholders. Thus, urban public policies are crucial to the improvement of the landscape ecological system, achievable by city planning and design. The paper aims to analyse if public urban policies in Portugal have been integrating strategies and/or guidelines to enhance the ecological system of the landscape. Then, which new perspectives are possible, framed by the recently approved law Bases of Public Policy of Soils, Land Management and Urban Planning (2014). This new law, in contrast with the previous ones, don’t allow reserving land to urbanize, in municipal master plans. Moreover, it is possible to revert land classified for urban purposes in those plans into rustic soils (when it is not yet infra-structured or built). It allows creating new planning and design dynamics, convert several areas and including them in the urban ecological structure, essential to the enhancement of landscape ecological system. This is a filed of work where landscape architecture has huge responsibilities, by associating and harmonize man-made environment with natural systems, enlightening sustainability consistent with conservation and improvement of Nature while contributing to the well-being and quality of life of Man. A sustainability that is ethical, aesthetic, ecological and cultural. The study is supported by a case study – the city of Évora. The ultimate goal is to propose measures to promote larger and better integration of ecological component in urban public policies, framed by the new territorial management law, taking into account and highlighting the specificities of the landscape system – Man and Nature – at the local level.

From Landscape to Garden-with Wildflowers Meadows

Since the middle age wildflower meadows are used to bring the flowers from the natural and rural areas into urban landscapes. Wildflowers meadows can improve the quality of Green Infrastructures as they increase biodiversity. However, after a few centuries of given flowers a principal part, lawns started to be used in every kind of places leading to a green obsession. Nowadays, urban lawns are cover more than 70% of urban green spaces all over the world. Frequently design options aren’t environmental or economical friendly, and lawns are this example. Lawns are green deserts with low biodiversity, and unsustainable. Wildflower meadows are an alternative to lawns, more sustainable, less resources consumer and much more biodiverse. In the regions with a Mediterranean climate water is a limit factor, especial in summer.

“ The Creation of an Urban Landscape” in Adaptive reuse,the modern movement toward the future, 14th international conference proceedings, Do_co.mo.mo international, Lisboa ( indexed by SCOPUS)

The headquarters and park of the Calouste Gulbenkian Foundation in Lisbon represent the first modern Portuguese environment with an outstanding relation between exterior and interior as a spatial continuum . As such, the project refused the more common conceptual attitude of interior plus exterior. This unitary view revealed a clear understanding of the proposed site for the project and what could have been Calouste Sarkis Gulbenkian (1869-1955) expectations, while concretizing Modern Movement ideals regarding landscape architecture and architecture. Through its design the park mediates the relation between the buildings’ super-structures, the urban context, and the human scale, while generating a unifying system established by the complicity between natural and synthetic materials. From the first moment of the project’s design process, this complicity resulted in a set of strategies that met programmatic

“The Landscape of wheat - A landscape of power” pp: 66-70

Portugal in the end of the 19 th century was characterized by a huge economic and political instability. This situation led to the publication of the Hunger Law (1899), which is identified as the critical breaking point since it imposed a political ideology over a vernacular landscape. The Alentejo (south of Portugal), between (1889-1929) emerges as a paradigmatic landscapes where an abrupt transformation take place: an ancient landscape with the loss of their ecological memory was lost and a false identities was created. The diversity and ecological richness of the vernacular landscape gave way to the monotony of the cereal and the ecological and social system that for centuries had built up and evolved was abruptly compromised. To analyze and understand the important transformation that happened in the ancestral countryside of Alentejo, the research was based on the concept of landscape as a system, and that the landscape represents the relationship between the natural system and the cultural.

Landslide activity in contrasting land-cover settings constrained by multi-temporal inventorying

This doctoral thesis focuses on the study of historical shallow landslide activity over time in response to anthropogenic forcing on land use, through the compilation of multi-temporal landslide inventories. The study areas, located in contrasting settings and characterized by different history of land-cover changes, include the Sillaro River basin (Italy) and the Tsitika and Eve River basins (coastal British Columbia). The Sillaro River basin belongs to clay-dominated settings, characterized by extensive badland development, and dominated by earth slides and earthflows. Here, forest removal began in the Roman period and has been followed by agricultural land abandonment and natural revegetation in recent time. By contrast, the Tsitika-Eve River basins are characterized by granitic and basaltic lithologies, and dominated by debris slides, debris flows and debris avalanches. In this setting, anthropogenic impacts started in 1960’s and have involved logging operation. The thesis begins with an introductory chapter, followed by a methodological section, where a multi-temporal mapping approach is proposed and tested at four landslide sites of the Sillaro River basin. Results, in terms of inventory completeness in time and space, are compared against the existing region-wide Emilia-Romagna inventory. This approach is then applied at the Sillaro River basin scale, where the multi-temporal inventory obtained is used to investigate the landslide activity in relation to historical land cover changes across geologic domains and in relation to hydro-meteorological forcing. Then, the impact of timber harvesting and road construction on landslide activity and sediment transfer in the Tsitika-Eve River basins is investigated, with a focus on the controls that interactions between landscape morphometry and cutblock location may have on landslide size-frequency relations. The thesis ends with a summary of the main findings and discusses advantages and limitations associated with the compilation of multi-temporal inventories in the two settings during different periods of human-driven, land-cover dynamics.

Impact of gentrification processes in rural landscapes: a methodology for landscape assessment towards sustainable rural development

With an increasing demand for rural resources and land, new challenges are approaching affecting and restructuring the European countryside. While creating opportunities for rural living, it has also opened a discussion on rural gentrification risks. The concept of rural gentrification encircles the influx of new residents leading to an economic upgrade of an area making it unaffordable for local inhabitants to stay in. Rural gentrification occurs in areas perceived as attractive. Paradoxically, in-migrants re-shape their surrounding landscape. Rural gentrification may not only cause displacement of people but also landscape values. Thus, this research aims to understand the twofold role of landscape in rural gentrification theory: as a possible driver to attract residents and as a product shaped by its residents. To understand the potential gentrifiers’ decision process, this research has provided a collection of drivers behind in-migration. Moreover, essential indicators of rural gentrification have been collected from previous studies. Yet, the available indicators do not contain measures to understand related landscape changes. To fill this gap, after analysing established landscape assessment methodologies, evaluating the relevance for assessing gentrification, a new Landscape Assessment approach is proposed. This method introduces a novel approach to capture landscape change caused by gentrification through a historical depth. The measures to study gentrification was applied on Gotland, Sweden. The study showed a population stagnating while the number of properties increased, and housing prices raised. These factors are not indicating positive growth but risks of gentrification. Then, the research applied the proposed Landscape Assessment method for areas exposed to gentrification. Results suggest that landscape change takes place on a local scale and could over time endanger key characteristics. The methodology contributes to a discussion on grasping nuances within the rural context. It has also proven useful for indicating accumulative changes, which is necessary in managing landscape values.

Patterns of long-term settlement and land exploitation in the region of the Nile First Cataract (Egypt)

The First Cataract region (Egypt) has always played a crucial role as a border area and a crossroads for cultures and people living in adjacent landscapes. The region has its central point in the modern city of Aswan, but it extends up to the Kom Ombo Plain in the north and reaches the Bab el-Kalabsha in the south. Its eastern and western limits cannot be defined with the same precision, given that they are located in deserts. This research focused on the landscape analysis of the region intended as a complex entanglement of archaeological evidence in a geographical and natural environment whose changes impacted and, simultaneously, were influenced by human activities. Settlement patterns and land use can give interesting information on how these relationships worked from a diachronic perspective and how they shaped the region’s characteristics. To understand the links between the human presence and its evidence and the landscape of the First Cataract region, the integration of various datasets was needed, from historical and archaeological ones to the remote sensing observation of large areas. An area corresponding to ca. 18.000 km2 has been selected for this research. The chronological framework has been chosen to cover a considerable period, from the beginning of the 5th millennium BCE to the 7th century AD. Multi-temporality and multifunctionality appear as two essential aspects when the archaeological evidence of the First Cataract region is considered in its geographical and topographical setting as a general context for settlement patterns and resource exploitation analyses. A combination of remote sensing data and topographical materials has been integrated with archaeological evidence to obtain information about resource exploitation strategies and settlement adaptation from a diachronic perspective.

Brazilian Natural Fiber (jute) As Raw Material For Activated Carbon Production.

Jute fiber is the second most common natural cellulose fiber worldwide, especially in recent years, due to its excellent physical, chemical and structural properties. The objective of this paper was to investigate: the thermal degradation of in natura jute fiber, and the production and characterization of the generated activated carbon. The production consisted of carbonization of the jute fiber and activation with steam. During the activation step the amorphous carbon produced in the initial carbonization step reacted with oxidizing gas, forming new pores and opening closed pores, which enhanced the adsorptive capacity of the activated carbon. N2 gas adsorption at 77K was used in order to evaluate the effect of the carbonization and activation steps. The results of the adsorption indicate the possibility of producing a porous material with a combination of microporous and mesoporous structure, depending on the parameters used in the processes, with resulting specific surface area around 470 m2.g-1. The thermal analysis indicates that above 600°C there is no significant mass loss.

Comparing Near-infrared Conventional Diffuse Reflectance Spectroscopy And Hyperspectral Imaging For Determination Of The Bulk Properties Of Solid Samples By Multivariate Regression: Determination Of Mooney Viscosity And Plasticity Indices Of Natural Rubber.

Conventional reflectance spectroscopy (NIRS) and hyperspectral imaging (HI) in the near-infrared region (1000-2500 nm) are evaluated and compared, using, as the case study, the determination of relevant properties related to the quality of natural rubber. Mooney viscosity (MV) and plasticity indices (PI) (PI0 - original plasticity, PI30 - plasticity after accelerated aging, and PRI - the plasticity retention index after accelerated aging) of rubber were determined using multivariate regression models. Two hundred and eighty six samples of rubber were measured using conventional and hyperspectral near-infrared imaging reflectance instruments in the range of 1000-2500 nm. The sample set was split into regression (n = 191) and external validation (n = 95) sub-sets. Three instruments were employed for data acquisition: a line scanning hyperspectral camera and two conventional FT-NIR spectrometers. Sample heterogeneity was evaluated using hyperspectral images obtained with a resolution of 150 × 150 μm and principal component analysis. The probed sample area (5 cm(2); 24,000 pixels) to achieve representativeness was found to be equivalent to the average of 6 spectra for a 1 cm diameter probing circular window of one FT-NIR instrument. The other spectrophotometer can probe the whole sample in only one measurement. The results show that the rubber properties can be determined with very similar accuracy and precision by Partial Least Square (PLS) regression models regardless of whether HI-NIR or conventional FT-NIR produce the spectral datasets. The best Root Mean Square Errors of Prediction (RMSEPs) of external validation for MV, PI0, PI30, and PRI were 4.3, 1.8, 3.4, and 5.3%, respectively. Though the quantitative results provided by the three instruments can be considered equivalent, the hyperspectral imaging instrument presents a number of advantages, being about 6 times faster than conventional bulk spectrometers, producing robust spectral data by ensuring sample representativeness, and minimizing the effect of the presence of contaminants.

Classificação de água de coco processada e natural por meio de HCA, PCA e teores de íons metálicos determinados por ICP OES

Inductively Coupled Plasma Optical Emission Spectrometry was used to determine Ca, Mg, Mn, Fe, Zn and Cu in samples of processed and natural coconut water. The sample preparation consisted in a filtration step followed by a dilution. The analysis was made employing optimized instrumental parameters and the results were evaluated using methods of Pattern Recognition. The data showed common concentration values for the analytes present in processed and natural samples. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) indicated that the samples of different kinds were statistically different when the concentrations of all the analytes were considered simultaneously.

Antimycobacterial and cytotoxicity activity of synthetic and natural compounds

Antimycobacterial and cytotoxicity activity of synthetic and natural compounds. Secondary metabolites from Curvularia eragrostidis and Drechslera dematioidea, Clusia sp. floral resin, alkaloids from Pilocarpus alatus, salicylideneanilines, piperidine amides, the amine 1-cinnamylpiperazine and chiral pyridinium salts were assayed on Mycobacterium tuberculosis H37Rv. N-(salicylidene)-2-hydroxyaniline was the most effective compound with a minimal inhibitory concentration (MIC) of 8 µmol/L. Dihydrocurvularin was moderately effective with a MIC of 40 µmol/L. Clusia sp. floral resin and a gallocatechin-epigallocatechin mixture showed MIC of 0.02 g/L and 38 µmol/L, respectively. The cytotoxicity was evaluated for N-(salicylidene)-2-hydroxyaniline, curvularin, dihydrocurvularin and Clusia sp. floral resin, and the selectivity indexes were > 125, 0.47, 0.75 and 5, respectively.

Tree structure and richness in an Atlantic Forest fragment: distance from anthropogenic and natural edges

Approximately 7.2% of the Atlantic rainforest remains in Brazil, with only 16% of this forest remaining in the State of Rio de Janeiro, all of it distributed in fragments. This forest fragmentation can produce biotic and abiotic differences between edges and the fragment interior. In this study, we compared the structure and richness of tree communities in three habitats - an anthropogenic edge (AE), a natural edge (NE) and the fragment interior (FI) - of a fragment of Atlantic forest in the State of Rio de Janeiro, Brazil (22°50'S and 42°28'W). One thousand and seventy-six trees with a diameter at breast height > 4.8 cm, belonging to 132 morphospecies and 39 families, were sampled in a total study area of 0.75 ha. NE had the greatest basal area and the trees in this habitat had the greatest diameter:height allometric coefficient, whereas AE had a lower richness and greater variation in the height of the first tree branch. Tree density, diameter, height and the proportion of standing dead trees did not differ among the habitats. There was marked heterogeneity among replicates within each habitat. These results indicate that the forest interior and the fragment edges (natural or anthropogenic) do not differ markedly considering the studied parameters. Other factors, such as the age from the edge, type of matrix and proximity of gaps, may play a more important role in plant community structure than the proximity from edges.