Activation mechanism of pre-phenoloxidase in lobster and shrimp

Phenoloxidase in shrimp and lobster is shown to exist in latent form which could be activated by trypsin and by an endogenous enzyme with tryptic activity. On Sephadex G-100 gel, three isoenzymes, differing in molecular weights, were isolated from naturally activated lobster shell extracts. Mechanism of activation of ptephenoloxidase involving limited proteolysis by the activating enzyme to form isoenzymes has been proposed.

Sampling plans for pre-packed fish and fish products

An outline is given of a sampling plan for prepacked fish products based on the net weight of the container, which may be used to make the inspection of fish and fish products easier and more effective.

Rate of filtration by Perna viridis pre-exposed to heavy metals

Perna viridis exposed to different concentrations of copper and zinc for varying periods and then the rate of filtration estimated under metal free culture conditions. The concentrations under which the animals were maintained before the experiments were 0.025 to 0.150 p.p.m. of zinc and 0.005 to 0.08 p.p.m. of copper.

Effect of pre-processing iced storage on deteriorative changes in lipids of silver pomfrets stored at -18°C

Silver pomfret (Pampus argenteus) was frozen in the fresh condition as well as after holding in ice for one, two and four days. Evaluation of changes in the quality of these samples during storage at -18°C has shown that shelf-life decreased sharply if the pre-freezing iced storage was more than one day. The shelf-life of one day iced, two day iced and four day iced frozen samples were 32, 20 and 16 weeks respectively. No correlation was observed between the peroxide value and the organoleptic detection of rancid flavour. Levels of free fatty acids were more in the samples frozen after storage in ice for one day than in all the other samples.

A pre-impoundment study of swamps in the Kainji Lake Basin

This paper gives an account of the wet season swamps in the River Niger valley within tbe area now submerged by the Kainji Lake. Their ecology was studied with respect to the soils, plant cover, water chemistry and plankton concentrations. Their value to the fishery in the river basin was discussed.

Impact of lubricant composition on low-speed pre-ignition

One of the limits on the maximum fuel efficiency benefit to be gained from turbocharged, downsized gasoline engines is the occurrence of pre-ignitions at low engine speed. These pre-ignitions may lead to high pressures and extreme knock (megaknock or superknock) which can cause severe engine damage. Though the mechanism leading to megaknock is not completely resolved, pre-ignitions are thought to arise from local autoignition of areas in the cylinder which are rich in low ignition delay "contaminants" such as engine oil and/or heavy ends of gasoline. These contaminants are introduced to the combustion chamber at various points in the engine cycle (e.g. entering from the top land crevice during blow-down or washed from the cylinder walls during DI wall impingement). This paper presents results from tests in which model "contaminants", consisting of engine lubricant base stocks, base stocks mixed with fuel and base stocks mixed with one or more additives were injected directly into a test engine to determine their propensity to ignite. The ignition tendency was found to be lower for less reactive base stocks and for base stocks mixed with certain additives. Further, when small amounts of fuel were mixed with relatively non-ignitive lubricant base stocks the ignition tendency was found to increase significantly. These results may guide development of new lubricants which could be used to reduce megaknock in downsized engines. Copyright © 2014 SAE International.

PURIFICATION AND CHARACTERIZATION OF A KINASE SPECIFIC FOR THE SERINE-RICH AND ARGININE-RICH PRE-MESSENGER-RNA SPLICING FACTORS

Members of the SR family of pre-mRNA splicing factors are phosphoproteins that share a phosphoepitope specifically recognized by monoclonal antibody (mAb) 104. Recent studies have indicated that phosphorylation may regulate the activity and the intracellular localization of these splicing factors. Here, we report the purification and kinetic properties of SR protein kinase 1 (SRPK1), a kinase specific for SR family members. We demonstrate that the kinase specifically recognizes the SR domain, which contains serine/arginine repeats. Previous studies have shown that dephosphorylated SR proteins did not react with mAb 104 and migrated faster in SDS gels than SR proteins from mammalian cells. We show that SRPK1 restores both mobility and mAB 104 reactivity to a SR protein SF2/ASF (splicing factor 2/alternative splicing factor) produced in bacteria, suggesting that SRPK1 is responsible for the generation of the mAb 104-specific phosphoepitope in vivo. Finally, we have correlated the effects of mutagenesis in the SR domain of SF2/ASF on splicing with those on phosphorylation of the protein by SRPK1, suggesting that phosphorylation of SR proteins is required for splicing.

Electron irradiation-induced defects in InP pre-annealed at high temperature

Electron irradiation-induced deep level defects have been studied in InP which has undergone high-temperature annealing in phosphorus and iron phosphide ambients, respectively. In contrast to a high concentration of irradiation-induced defects in as-grown and phosphorus ambient annealed InP, InP pre-annealed in iron phosphide ambient has a very low concentration of defects. The phenomenon has been explained in terms of a faster recombination of radiation-induced defects in the annealed InP. The radiation-induced defects in the annealed InP have been compared and studied. (c) 2006 Elsevier Ltd. All rights reserved.

Silicon nanowires grown on a pre-annealed Si substrate

Polycrystalline Si nanowires (poly SiNWS) were successfully synthesized by plasma-enhanced chemical vapor deposition (PECVD) at 440degreesC using silane as the Si source and Au as the catalyst. The diameters of Si nanowires range from 15 to 100nm. The growth process indicates that to fabricate SiNWS by PECVD, pre-annealing at high temperature is necessary. A few interesting nanowires with Au nanoclusters uniformly distributed in the body of the wire were also produced by this technique. (C) 2002 Elsevier Science B.V. All rights reserved.