APPLICATIONS OF RAPID SCAN SPECTROELECTROCHEMISTRY - DETERMINATION OF HETEROGENEOUS ELECTRON-TRANSFER RATE-CONSTANT OF BILIVERDIN OXIDATION

The rapid scan spectrometer was used to determine the heterogeneous electron transfer rate parameters for the oxidation of Biliverdin in DMF by single potential step thin layer spectroelectrochemical techniques and yielded an average formal heterogeneous electron transfer rate constant K(s, h)0' = 2.45 (+/-0.12) x 10(-4) cm s-1, electrochemical transfer coefficient alpha = 0.694+/-0.008. The oxidation process of Biliverdin was also studied and the formal potential E0 = 0.637 V (vs. Ag/AgCl) was obtained.

Na_3AlF_6-LiF-RE_2O_3体系表面张力的研究

用最大气泡压力法,采用三因子一次回归正交设计,研究了Na_3AlF_6-LiF-RE_2O_3体系的表面张力,得出表面张力(Y)与RE_2O_3含量(c_1)、LiF含量(c_2)及溶体温度(t)的回归方程如下:Y=260.78+4.088c_1+0.073c_2+0.557c_1c_2-0.136t-0.00483c_1t-0.00050c_2t。

A WOUND-TYPE LITHIUM POLYANILINE SECONDARY CELL

A wound-type cell with a polyaniline (PAn) positive electrode, a LiClO4-propylene carbonate (PC) electrolyte, and a lithium foil negative electrode has been constructed. The two electrodes are separated by a polypropylene separator. The PAn is deposited on carbon felt from a HClO4 solution containing aniline by galvanostatic or potentiostatic electrolysis. Using cyclic voltammetry charge/discharge cycles and charge/retention tests, the following results have been obtained: (i) reversibility of the charge/discharge reaction of the PAn electrode is very good; (ii) more than 50 charge/discharge cycles at 80% charge/discharge efficiency and 260 W h kg-1 discharge energy density can be achieved at 50 mA between 2 and 4 V; (iii) the open-circuit voltage and the capacity retention of the battery after storage at open-circuit for 60 days are 3.4 V and 33%, respectively.

AN ALGEBRAIC METHOD TO ESTIMATE GENERAL TYPE OF PHASES OF STRUCTURE FACTORS

The algebraic formulas of 1.5 and 2.5 rank are given for four space groups P2(1), Pn, Pna2(1), P2(1)2(1)2(1). It is better that the results of applying them to estimating general type of phases for four correspondent crystal structures. And a method of transforming algebraic formulas from 1.5(2.5) rank is proposed.

取代基效应与乙烯-α-烯烃共聚物的序列结构——5.乙烯-乙烯醇共聚物

本文应用取代基效应(SCS)方法研究了乙烯-乙烯醇(EVA)共聚物,得到了羟基(-OH)在两种不同溶剂中的SCS参数:在苯酚+重水(80/20W/W)中参数为S_1=42.77±0.08ppm,S_2=7.155±0.06ppm,S_3(s)=-4.08±0.02ppm,S_3(t)=-3.09±0.02ppm,S_4=0.48±0.03ppm,S_5=0.26±0.05ppm,在以邻二氯苯为溶剂时参数为S_1=44.97±0.61ppm,S_2=7.40±0.00ppm,S_3(s)=-4.51±0.17ppm,S_3(t)=-3.13±0.00ppm,S_4=0.63±0.04ppm,S_5=0.36±0.00ppm,同时利用所得到的SCS参数对该共聚物的~(13)C NMR谱进行了归属。

一般型相角的代数估算方法

在晶体结构测定的直接法相角问题研究中,迄今为止人们研究得最多的仍是特殊类型相角(0,π型,±π/2型),尤其那些符合结构半不变量要求的0,π型相角更是人们注意的焦点.而一般型相角由于取值范围广(0—360°), 数量多,研究中涉及较多的理论和实际问题,故通常认为估算此类相角将有更大困难.本文利用我们在±π/2相角研究中已经导出的1.5和2.5阶代数式,较好地实现了一般型相角的估算。

SYNTHESIS AND MOLECULAR-STRUCTURE OF THE 5-COORDINATE ZIRCONIUM COMPLEX (CH3C5H4)2ZRCL(ALPHA-C10H7COO)

Bis(methylcyclopentadienyl)chloro(alpha-naphthoyloxy)zirconium, (CH3C5H4)2Zr Cl(alpha-C10H7COO), has been synthesized by the reaction between (MeCp)2ZrCl2 and equimolar sodium alpha-naphthoate in toluene at room temperature. The molecular structure of the title complex was determined by X-ray diffraction. The molecules have a five-coordinate bent metallocene structure in which the alpha-naphthoxyligand is bidentate [Zr-O, 2.317(2), 2.260(2) angstrom]. The remaining distances are [Zr-Cl, 2.521(1) angstrom; Zr-C, 480-2, 565 angstrom; Zr-cent-CH3C5H4 2.217 and 2.230 angstrom].

氮丙啶衍生物的稳定性和固化反应机理的~13C-NMR研究

本文用~(13)C-NMR方法研究了间苯二甲酰二(2-甲基氮丙啶)和癸二酰二(2-甲基氮丙啶)的稳定性和固化反应机理。在常温下,这两种化合物均可缓慢重排为(口恶)唑啉,也可同空气中水发生水解反应。在重排和固化反应过程中,它们的N-CH和N-CH_2键断裂情况是不同的。

激光引发Cl_2和CH_2Cl_2对Cu的化学刻蚀

本文利用氩离子激光引发的化学反应,对铜片进行刻蚀研究,给出了刻蚀速率与激光能量和工作气体压强的关系曲线,得到 Cu—Cl_2体系的光刻能量阀值低于 Cu—CH_2Cl_2体系的结果并讨论了这一差别的原因。

Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)

RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 mu g g(-1) fresh weight) and high quality (A (260/280) ratio 1.96 +/- 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.

A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica

A simple method was developed for extracting DNA from brown algae Laminaria japonica, which possess large amounts of acidic polysaccharides. Firstly, the sporophyte were washed by eliminating polysaccaride buffer to remove the polysaccharides and then ground in liquid nitrogen. Secondly, the powders were treated with lysing buffer. Thirdly, KAc was used to eliminate the remaining acidic polysaccharides. The extracted DNA was purified using a chloroform-isoamyl alcohol ( 24: 1 v/v), and precipitated in cold isopropanol. The yield was from 18.7 to 37.5 mu g g(-1) (wet weight) and the purity of total DNA was determined spectrophotometrically as the ratio of A(260)/A(280), which was about 1.7 - 1.9. The extracted DNA was of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion. This method is a reproducible, simple, and rapid technique for routine DNA extraction from sporophyte in Laminaria japonica. Furthermore, the low cost of this method makes it attractive for large-scale studies.

An efficient method for DNA isolation from red algae

A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation.

Molecular cloning and characterization of proliferating cell nuclear antigen (PCNA) from Chinese shrimp Fenneropenaeus chinensis

The proliferating cell nuclear antigen gene was cloned from Fenneropenaeus chinensis (FcPCNA). The full-length cDNA sequence of FcPCNA encodes 260 amino acids showing high identity with PCNAs reported in other species. FcPCNA expressed especially high in proliferating tissues of shrimp such as haematopoietic tissue (HPT) and ovary. In order to understand the response of HPT to bacteria and virus challenge, mRNA level of FcPCNA in HPT was analyzed after shrimp were challenged by Vibrio anguillarum and white spot syndrome virus (WSSV). FcPCNA expression in HPT of shrimp was responsive to WSSV and Vibrio challenge, but different expression profiles were obtained after challenge by these two pathogens. The data provide additional information to understand the defense mechanisms of shrimp against virus and bacteria. (c) 2008 Elsevier Inc. All rights reserved.

Evidences of the intertidal red alga Grateloupia turuturu in turning Vibrio parahaemolyticus into non-culturable state in the presence of light

Grateloupia turuturu, previously known as Grateloupia doryphora, has been widely reported to be an invasive algal species. There are no studies to relate the impact of its existence on its surrounding environment. In this paper, we present our results to show that about 70% of individuals collected from the field could turn Vibrio parahaemolyticus into non-culturable state on both selective (TCBS) and non-selective (2216E) culture medium in 24 h in the presence of light in live algal culture. Total bacteria counts on TCBS and 2216E plates dropped from the initial 565 (174) and 1192 (60) cfu ml(-1) respectively to zero in 24 h. This effect disappeared when the alga was grown in darkness. The same effect was not found in two other intertidal macroalgae Laminaria japonica and Palmaria palmata. Further tests showed that the settlement ability of bacteria in seawater was impaired significantly in the presence of this alga in comparison with three other algal species. (c) 2006 Elsevier B.V. All rights reserved.