Mapping of a gene coding for a major late structural polypeptide on the vaccinia virus genome.

Cell-free translation of total RNA isolated from vaccinia virus-infected cells late in infection results in a complex mixture of polypeptides. A monospecific antibody directed against one of the major structural proteins of the virus particle immunoprecipitated a single polypeptide with a molecular weight of 11,000 (11K) from this mixture. Immunoprecipitation was therefore used to identify the structural polypeptide among the in vitro translation products of RNA purified by hybridization selection to restriction fragments of the vaccinia virus genome. This allowed us to map the mRNA coding for the 11K polypeptide to the extreme left-hand end of the HindIII E fragment. Detailed transcriptional mapping of this region of the genome by nuclease S1 analysis revealed the presence of a late RNA transcribed from the rightward-reading strand. Its 5' end mapped at ca. 130 base pairs to the left of the HindIII site at the junction between the HindIII F and E fragments. The map position of this RNA coincided precisely with the map position of the late message coding for the 11K polypeptide.

Liver, Gastrointestinal, and Cardiac Toxicity in Intermediate Hepatocellular Carcinoma Treated With PRECISION TACE With Drug-Eluting Beads: Results From the PRECISION V Randomized Trial.

OBJECTIVE. The purpose of our study was to evaluate hepatic, gastrointestinal, and cardiac toxicity after PRECISION transarterial chemoembolization (TACE) with drug-eluting beads (DEB) versus conventional TACE with doxorubicin in the treatment of intermediate-stage hepatocellular carcinoma (HCC).SUBJECTS AND METHODS. Two hundred twelve patients (185 men and 27 women; mean age, 67 years) were randomized to TACE with DEB or conventional TACE. The majority of patients (67% in both groups) presented in a more advanced stage. Safety was measured by rate of adverse events (Southwest Oncology Group criteria) and changes in laboratory parameters. Cardiotoxicity was assessed with left ventricular ejection fraction (LVEF) mainly on MRI or echocardiography.RESULTS. The mean maximum postchemoembolization alanine transaminase increase in the DEB group was 50% less than in the conventional TACE group (p < 0.001) and 41% less in respect to aspartate transaminase (p < 0.001). End-of-study values returned to approximately baseline levels but with greater variability in conventional TACE patients. Treatment-emergent adverse events in the hepatobiliary system organ class occurred in 16.1% of DEB group patients compared with 25% of conventional TACE patients. There were fewer liver toxicity events in the DEB group. There was a small but statistically significant difference in mean change from baseline in LVEF between the two groups of 4 percentage points for the conventional TACE group (95% CI, 0.71-7.3; p = 0.018).CONCLUSION. PRECISION TACE with DEB loaded with doxorubicin offers a safe therapy option for intermediate-stage HCC, even in patients with more advanced liver disease.

Hacia una formación europea en educación social

En el marco de la EEES, en el que se hace hincapié en la dimensión internacional de los estudios universitarios, el uso de las tecnologías de la comunicación y la autonomía del trabajo del estudiante, diversos centros dedicados a la formación de Educadores sociales, junto con empresas del sector socioeducativo se reúnen para pensar las bases de lo que podría ser una formación europea en Educación Social. En un proceso de más de cinco años de trabajo se ha consolidado un proyecto de formación y de intercambio entre estudiantes profesores y profesionales de la educación social con el objetivo de favorecer la apertura intercultural y la capacidad de trabajar en contextos interprofesionales, en una red internacional. Un proyecto que se inicia con una propuesta formativa centrada en unas prácticas de un mínimo de tres meses en un país extranjero con unos complementos de formación, evoluciona hacia otras formas más flexibles de intercambio en las que se incorporan profesores y profesionales que acompañan a estudiantes en prácticas.

Some aspects of the neuronal cytoskeleton in development.

The review is focused on developmental aspects of the neuronal cytoskeleton, its molecular composition and the intracellular distribution of its elements. It includes a survey of the molecular properties of several cytoskeletal proteins such as tubulins, microtubule-associated proteins, neurofilament subunits, actins and brain spectrins. Furthermore it is addressed how microtubules, neurofilaments, microfilaments and the spectrin-based membrane cytoskeleton are involved in the generation of the neuronal cytoarchitecture, and how changes in the molecular composition of the cytoskeleton during the differentiation process of a neuron may correlate with cell function.

El trabajo en equipo, la planificación y la concertación de servicios en la práctica profesional

Con el título “El trabajo en equipo, la planificación y concertación de servicios en la práctica profesional” se presenta la experiencia formativa que se desarrolla en el segundo curso de la Diplomatura de Educación Social con la intención de que los estudiantes investiguen y reflexionen sobre la práctica de la planificación socioeducativa en un contexto de concurso público para la concertación de un servicio.

El Medio educativo como generador de satisfacciones vitales para favorecer un envejecimiento activo en un programa universitario de mayores .

Desarrollar nuevas innovaciones educativas en un Programa Universitario para mayores (PUGG) que favorezca un envejecimiento activo y significativo en los mayores.

Simulación de concurso público como innovación docente

La presente experiencia se ubica en el marco de prácticum (espacio de grupo reducido de estudiantes y teórico-práctico) de la diplomatura de educación social de la Facultad de Educación Social y Trabajo Social Pere Tarrés (Universidad Ramon Llull). La propuesta se hizo de manera simultánea en cada uno de los 5 grupos que conforman todo el segundo curso de esta titulación. La propuesta presentada constituía uno de los trabajos de esta asignatura. El estudiante realizó también un trabajo individual que no describiremos. El encargo consistía en la búsqueda y diseño de instrumentos para la gestión socioeducativa de una casa de acogida para mujeres en situación de maltrato con el fin de optimizar el funcionamiento del dispositivo. El encargo de estas herramientas se hace bajo un supuesto generalista para este tipo de dispositivos sociales, teniendo en cuenta que pueden haber otras herramientas pero que, en todo caso, éstas son las fundamentales o las más utilizadas. Las herramientas que se pidieron fueron las siguientes: Proyecto Educativo,Reglamento de Régimen Interno, Proyecto Educativo Individualizado, Evaluación y Memoria, Programas, Protocolos (entrada y salida), Herramientas de coordinación, y Expediente Educandos.

Creatividad y relación interpersonal en el proceso de evaluación

Un planteamiento inadecuado de una actividad de evaluación propició un cambio radical en la manera de plantear la relación pedagógica aplicada a la evaluación por parte del profesor de la asignatura

Determining the best population-level alcohol consumption model and its impact on estimates of alcohol-attributable harms.

BACKGROUND: The goals of our study are to determine the most appropriate model for alcohol consumption as an exposure for burden of disease, to analyze the effect of the chosen alcohol consumption distribution on the estimation of the alcohol Population- Attributable Fractions (PAFs), and to characterize the chosen alcohol consumption distribution by exploring if there is a global relationship within the distribution. METHODS: To identify the best model, the Log-Normal, Gamma, and Weibull prevalence distributions were examined using data from 41 surveys from Gender, Alcohol and Culture: An International Study (GENACIS) and from the European Comparative Alcohol Study. To assess the effect of these distributions on the estimated alcohol PAFs, we calculated the alcohol PAF for diabetes, breast cancer, and pancreatitis using the three above-named distributions and using the more traditional approach based on categories. The relationship between the mean and the standard deviation from the Gamma distribution was estimated using data from 851 datasets for 66 countries from GENACIS and from the STEPwise approach to Surveillance from the World Health Organization. RESULTS: The Log-Normal distribution provided a poor fit for the survey data, with Gamma and Weibull distributions providing better fits. Additionally, our analyses showed that there were no marked differences for the alcohol PAF estimates based on the Gamma or Weibull distributions compared to PAFs based on categorical alcohol consumption estimates. The standard deviation of the alcohol distribution was highly dependent on the mean, with a unit increase in alcohol consumption associated with a unit increase in the mean of 1.258 (95% CI: 1.223 to 1.293) (R2 = 0.9207) for women and 1.171 (95% CI: 1.144 to 1.197) (R2 = 0. 9474) for men. CONCLUSIONS: Although the Gamma distribution and the Weibull distribution provided similar results, the Gamma distribution is recommended to model alcohol consumption from population surveys due to its fit, flexibility, and the ease with which it can be modified. The results showed that a large degree of variance of the standard deviation of the alcohol consumption Gamma distribution was explained by the mean alcohol consumption, allowing for alcohol consumption to be modeled through a Gamma distribution using only average consumption.

In vivo localisation of radiolabelled monoclonal antibody in human gliomas.

F(ab')2-fragments of the anti-melanoma monoclonal antibody MeI-14 were labelled with 123I for external scanning and with 125I for tissue measurement of radioactivity and injected intravenously into patients scheduled for surgical resection of a glioma. The paired-label study was performed by injecting simultaneously 131I-labelled control (F(ab')2-fragments. The patients were scanned by computerised tomoscintigraphy. After surgery, the activities of 125I and 131I were counted in tumour and normal tissues. The results indicate that there was a low but definite uptake of the antibody in the tumour due to its specificity. The external detection was difficult because of accumulation of antibody fragments in the skull.

Activismo digital y social como innovación metodológica universitària

En la actualidad son numerosos los medios de interacción personal individual o entre organizaciones. La oferta de plataformas es prácticamente incontable y la oferta de contenidos es prácticamente infinita. La experiencia que presentamos pretende que los estudiantes universitarios tengan una experiencia de comunicación mediante las nuevas tecnologías con la finalidad de poder generar un trabajo colaborativo que posibilite, en parte, el cambio social.