Ca-Sr-Cu-O系中若干化合物的组成和结构的研究

研究了Ca-Sr-Cu-O三元金属复合氧化物体系,发现了两个新化合物Sr_3Cu_5O_(8+x)和CaSrCu_3·O_(5+x),两者都属正交晶系,前者a=3.950,b=11479,c=13.420 A.后者a=6.489,b=11.280,c=12.240A.文中给出了这两个化合物和SrCuO_2、Sr_2CuO_3的XRD谱.用碘量法测定了化合物中的氧含量.此外还发现了结构与SrCuO_2相似的固溶体Sr_(0.5)Ca_(0.5)CuO_2,也测定了它的XRD谱.

立规聚甲基丙烯酸热表征研究

本文对不同立构的聚甲基丙烯酸(PMAA)进行了热表征。实验结果表明,在30℃～200℃内未出现玻璃化转变。间同PMAA分别在-245℃和-280℃发生脱水反应和脱羧,而全同PMAA只在-185℃观察到脱水反应。这种热稳定性的明显差别与不同立构PMAA链上相邻羧酸距离有关。实验数据证实PMAA脱水反应为无规反应,服从一级动力学反应方程。脱水温度和间同立构含量成正比,脱水反应活化能间同PMAA比全同PMAA高～7Kcal/mol。

Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation

Insulin-like growth factor-binding protein (IGFBP)-3 is the major insulin-like growth factor (IGF) carrier protein in the bloodstream. IGFBP-3 prolongs the half-life of circulating IGFs and prevents their potential hypo-glycemic effect. IGFBP-3 is also expressed in many peripheral tissues in fetal and adult stages. In vitro, IGFBP-3 can inhibit or potentiate IGF actions and even possesses IGF-independent activities, suggesting that local IGFBP-3 may also have paracrine/autocrine function(s). The in vivo function of IGFBP-3, however, is unclear. In this study, we elucidate the developmental role of IGFBP-3 using the zebrafish model. IGFBP-3 mRNA expression is first detected in the migrating cranial neural crest cells and subsequently in pharyngeal arches in zebrafish embryos. IGFBP-3 mRNA is also persistently expressed in the developing inner ears. To determine the role of IGFBP-3 in these tissues, we ablated the IGFBP-3 gene product using morpholino-modified antisense oligonucleotides (MOs). The IGFBP-3 knocked down embryos had delayed pharyngeal skeleton morphogenesis and greatly reduced pharyngeal cartilage differentiation. Knockdown of IGFBP-3 also significantly decreased inner ear size and disrupted hair cell differentiation and semicircular canal formation. Furthermore, reintroduction of a MO-resistant form of IGFBP-3 "rescued" the MO-induced defects. These findings suggest that IGFBP-3 plays an important role in regulating pharyngeal cartilage and inner car development and growth in zebrafish.

Responses of vegetative gametophytes of Undaria pinnatifida to high irradiance in the process of gametogenesis

The population of Undaria pinnatifida in its ecologic niche sustains itself in high temperature summer in the form of vegetative gametophytes, the haploid stage in its heteromorphic life cycle. Gametogenesis initiates when seawater temperature drops below the threshold levels in autumn in the northern hemisphere. Given that the temperature may fall into the appropriate range for gametogenesis, the level of irradiance determines the final destiny of a gametophytic cell, either undergoing vegetative cell division or initiating gametogenesis. In elucidating how vegetatively propagated gametophytes cope with changes of irradiance in gametogenesis, we carried out a series of culture experiments and found that a direct exposure to irradiance as high as 270 mu mol photons m(-2) s(-1) was lethal to dim-light (7-10 mu mol photons m(-2) s(-1)) adapted male and female gametophytes. This lethal effect was linearly corelated with the exposure time. However, dim-light adapted vegetative gametophytes were shown to be able tolerate as high as 420 mu mol photons m(-2) s(-1) if the irradiance was steadily increased from dim light levels (7-10 mu mol photons m(-2) s(-1)) to 90, 180 and finally 420 mu mol photons m(-2) s(-1), respectively, at a minimum of 1-3 h intervals. Percentage of female gametophytic cells that turned into oogonia and were eventually fertilized was significantly higher if cultured at higher but not lethal irradiances. Findings of this investigation help to understand the dynamic changes of population size of sporophytic plants under different light climates at different site-specific ecologic niches. It may help to establish specific technical details of manipulation of light during mass production of seedlings by use of vegetatively propagated gametophytes.

Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)

RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 mu g g(-1) fresh weight) and high quality (A (260/280) ratio 1.96 +/- 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.

One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata

Phycoerythrins have been widely used in food, cosmetics., immunodiagnostics and analytical reagents. An efficient one-step chromatography method for purification of R-phycoerythrins from Polysiphonia urceolata was described in this paper. Pure R-phycoerythrin was obtained with an absorbance ratio A(565)/A(280) of 5.6 and a high recovery yield of 67-33%, using a DEAE-Sepharose Fast Flow chromatography with a gradient elution of pH, alternative to common gradient elution of ionic strength. The absorption spectrum of R-phycoerythrin was characterized with three absorbance maxima at 565, 539 and 498 mum, respectively and the fluorescence emission spectrum at room temperature was measured to be 580nm. The results of native-PAGE. and SDS-PAGE showed no contamination by other proteins in the phycoerythrin solution. which suggests an efficient method for the separation and purification of R-phycoerythrins from Polysiphonia urceolata. (C) 2004 Elsevier B.V. All rights reserved.

A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica

A simple method was developed for extracting DNA from brown algae Laminaria japonica, which possess large amounts of acidic polysaccharides. Firstly, the sporophyte were washed by eliminating polysaccaride buffer to remove the polysaccharides and then ground in liquid nitrogen. Secondly, the powders were treated with lysing buffer. Thirdly, KAc was used to eliminate the remaining acidic polysaccharides. The extracted DNA was purified using a chloroform-isoamyl alcohol ( 24: 1 v/v), and precipitated in cold isopropanol. The yield was from 18.7 to 37.5 mu g g(-1) (wet weight) and the purity of total DNA was determined spectrophotometrically as the ratio of A(260)/A(280), which was about 1.7 - 1.9. The extracted DNA was of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion. This method is a reproducible, simple, and rapid technique for routine DNA extraction from sporophyte in Laminaria japonica. Furthermore, the low cost of this method makes it attractive for large-scale studies.

An efficient method for DNA isolation from red algae

A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation.

Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography

C-phycocyanin was purified on a large scale by a combination of expanded bed adsorption, anion-exchange chromatography and hydroxyapatite chromatography from inferior Spirulina platensis that cannot be used for human consumption. First, phycobiliproteins were extracted by a simple, scaleable method and then were recovered by Phenyl-Sepharose chromatography in an expanded bed column. The purity (the A(620)/A(280) ratio) of C-phycocyanin isolated with STREAMLINE (TM) Column was up to 2.87, and the yield was as high as 31 mg/g of dried S. platensis. After the first step, we used conventional anion-exchange chromatography for the purification steps, with a yield of 7.7 mg/g of dried S. platensis at a purity greater than 3.2 and with an A(620)/A(650) index higher than 5.0. The fractions from anion-exchange chromatography with a level of purity that did not conform to the above standard were subjected to hydroxyapatite chromatography, with a C-PC yield of 4.45 mg/g of dried S. platensis with a purity greater than 3.2. The protein from both purification methods showed one absolute absorption peak at 620 nm and a fluorescence maximum at 650 nm, which is consistent with the typical spectrum of C-phycocyanin. SDS-PAGE gave two bands corresponding to 21 and 18 kDa. In-gel digestion and LC-ESI-MS showed that the protein is C-phycocyanin. (c) 2006 Elsevier B.V. All rights reserved.

Axial growth of hexactinellid spicules: Formation of cone-like structural units in the giant basal spicules of the hexactinellid Monorhaphis

The glass sponge Monorhaphis chuni (Porifera: Hexactinellida) forms the largest bio-silica structures on Earth; their giant basal spicules reach sizes of up to 3 m and diameters of 8.5 mm. Previously, it had been shown that the thickness growth proceeds by appositional layering of individual lamellae; however, the mechanism for the longitudinal growth remained unstudied. Now we show, that the surface of the spicules have towards the tip serrated relief structures that are consistent in size and form with the protrusions on the surface of the spicules. These protrusions fit into the collagen net that surrounds the spicules. The widths of the individual lamellae do not show a pronounced size tendency. The apical elongation of the spicule proceeds by piling up cone-like structural units formed from silica. As a support of the assumption that in the extracellular space silicatein(-like) molecules exist that associate with the external surface of the respective spicule immunogold electron microscopic analyses were performed. With the primmorph system from Suberites domuncula we show that silicatein(-like) molecules assemble as string- and net-like arrangements around the spicules. At their tips the silicatein(-like) molecules are initially stacked and at a later stay also organized into net-like structures. Silicatein(-like) molecules have been extracted from the giant basal spicule of Monorhaphis. Applying the SDS-PAGE technique it could be shown that silicatein molecules associate to dimers and trimers. Higher complexes (filaments) are formed from silicatein(-like) molecules, as can be visualized by electron microscopy (SEM). In the presence of ortho-silicate these filaments become covered with 30-60 nm long small rod-like/cuboid particles of silica. From these data we conclude that the apical elongation of the spicules of Monorhaphis proceeds by piling up cone-like silica structural units, whose synthesis is mediated by silicatein(-like) molecules. (C) 2008 Elsevier Inc. All rights reserved.

Fine sediment resuspension dynamics in Moreton Bay

A comprehensive field study has been undertaken to investigate sediment resuspension dynamics in the Moreton Bay, a large semi-enclosed bay situated in South East Queensland, Australia. An instrumented tripod, which housed three current meters, three OBS sensors and one underwater video camera, was used to collect the field data on tides, currents, waves and suspended sediment concentrations at four sites (Sites 1, 2, 4, and 5) in the bay. Site I was located at the main entrance, Site 2 at the central bay in deep water, and Sites 4 and 5 at two small bays in shallow water. The bed sediment was fine sand (d(50) = 0. 2 mm) at Site 1, and cohesive sediment at the other three sites. Based on the collected field data, it is found that the dominant driving forces for sediment resuspension are a combination of ocean swell and tidal currents at Site 1, tidal currents at Site 2, and wind-waves at Sites 4 and 5. The critical bed shear stress for cohesive sediment resuspension is determined as 0. 079 Pa in unidirectional flow at Site 2, and 0. 076 Pa in wave-induced oscillatory flow at Site 5.

蓝藻信号转导系统的比较基因组学分析及重要基因功能的验证

蓝藻经过漫长的进化，在地球上具有极其广范的分布，而且对环境胁迫具有极强的耐受性，这其中信号转导系统起了至关重要的作用。蓝藻信号转导系统主要包括二元信号转导系统和丝氨酸/苏氨酸激酶。蓝藻全基因组测序工作的完成为蓝藻信号转导系统的研究奠定了基础。目前国外对蓝藻信号转导系统的研究主要集中在对单个蓝藻的单个信号系统的功能研究。本论文首先采用比较基因组学的方法对蓝藻中的两大信号转导系统从分布、结构及进化等角度进行了系统的分析，进而对其中重要信号转导基因的功能进行了验证。 通过比较基因组学分析发现蓝藻中的信号转导系统的分布、结构特征与物种的生理、生态特征之间关系密切；蓝藻中丝氨酸/苏氨酸激酶具有与真核型激酶类似的催化方式；在信号转导系统的进化过程中存在着基因复制、缺失、附属功能域的获得及随机重排等复杂的进化现象；且二元信号转导系统中相互作用的组氨酸激酶与反应调控蛋白之间并不是完全同步的进化关系，可能有着不同的进化历程。该研究建立了蓝藻信号转导系统中的基因-结构-功能的框架结构，为其功能的研究奠定了基础。 聚球藻PCC7942和集胞藻PCC6803是单细胞的淡水蓝藻，具有天然的外源DNA转化系统，是蓝藻分子遗传学研究的模式生物。通过基因突变的方法对这两株蓝藻中二元信号转导系统的部分重要反应调控蛋白同源基因rre28和syn7942_0095的功能进行了验证，发现高度保守的同源序列在这两株蓝藻中起着不同的作用，且一个组氨酸激酶可能与不同的反应调控蛋白相互作用，说明同源序列的功能在进化的过程中已经发生了分化。 目前对蓝藻信号转导系统中丝氨酸/苏氨酸激酶的功能研究较少。通过基因突变及表达差异分析发现集胞藻PCC6803中的丝氨酸/苏氨酸激酶SpkG参与高盐胁迫的信号传递。通过高盐胁迫条件下SpkG对整个转录图谱影响的研究，发现了60个差异表达基因，涉及转运、能量传递、蛋白加工修饰和信号转导等多个生理过程。该研究首次通过实验验证了蓝藻中的丝氨酸/苏氨酸激酶对环境胁迫的响应，并首次发现由二元信号转导系统和丝氨酸/苏氨酸激酶共同参与的对逆境胁迫的调控网络。 蓝藻兼具细菌和植物的特点，蓝藻成熟的转化体系也为真核生物基因功能的研究提供了新的模式宿主。蓝藻信号转导系统中激酶功能的研究为我们进一步研究真核生物激酶的功能提供借鉴，且对蓝藻信号转导的研究将对植物的抗逆胁迫研究提供重要的理论依据。

菲律宾蛤仔（Ruditapes philippinarum）对胶州湾生态环境影响的现场研究

本文对我国胶州湾养殖的主要滤食性贝类菲律宾蛤仔的生理生态学进行了系统研究，通过资料收集、现场调查、定点连续观测、现场模拟实验等综合性方法探讨了菲律宾蛤仔养殖对胶州湾生态环境的影响。主要系统调查了不同季节胶州湾自然沉积物中C、N和P的变化；研究了菲律宾蛤仔生物沉积作用的周年变化；同时还研究了不同月份菲律宾蛤仔的耗氧率，排泄率的变化。结果如下: 1、综述了滤食性贝类通过滤食、生物沉积和呼吸排泄等作用对海区环境的影响,系统评述了埋栖性贝类生物沉积的测定方法及其生态效应。国际上已有不少研究专门报道了贝类在海区现场的生物沉积作用，而在我国，关于埋栖性贝类生物沉积特征的研究较少。 2、在胶州湾红岛附近养殖海区，从2003年10月到2004年7月以及2005年8月到2005年12月对菲律宾蛤仔生物沉积进行了现场测定。大、中和小规格菲律宾蛤仔生物沉积速率范围分别为121.6～1527.4mg/ind·d，49.5～902.1mg/ind·d，14.2～653.9mg/ind·d。海底自然沉积物OM分别为3.1％和5.7±1.1％。而大、中和小规格菲律宾蛤仔生物沉积物的OM分别为6.2±1.2％、5.9±1.3％和6.1±1.4％。大、中和小菲律宾蛤仔生物沉积物TP含量分别为399±26ppm，372±16ppm，345±15ppm。大、中和小规格蛤子生物沉积物中的OP分别为80±17ppm，92±12ppm和102±10ppm，明显高于对照沉积物的OP。生物沉积物中的OP/TP要明显高于对照沉积物，前者为23～25％，而后者仅为20％。菲律宾蛤仔生物沉积速率呈明显季节性变化，其与软体干重呈异速方程关系，a值的变化范围为0.85～4.50（平均为2.32）。菲律宾蛤仔贝肉的OC和ON含量分别为40.80±7.59％和的10.26±2.19％。贝壳的OM、TP、OP、OC和ON含量分别为3.28±0.47%、109.2±16.6ppm、64±22.9ppm、12.21±0.30％和0.19±0.05％。 3、不同规格菲律宾蛤仔的代谢率呈明显季节变化。7月份，单位个体菲律宾蛤仔耗氧率最高为1.72 g/ind•h；8月份，排氨率和排磷率达到最高，分别为2.44μmol/ind•h、0.58μmol/ind•h。菲律宾蛤仔的O:N在10.7~31.9范围内，O:N和N:P具有明显的季节变化，8月份最大。 4、研究了本湾底质环境特征，测定了自然沉积物的OM、OC、ON、TP和OP的含量。胶州湾站位沉积物的有机质和C、N、P含量季节变化不大，有机质含量大约为3.5％，OC和ON含量大约为0.7％和0.06％。TP含量为285ppm左右，OP含量大约为50ppm。养殖海区沉积物有机质和C、N、P的含量明显高于非养殖海区沉积物含量，其C/N、C/OP和OP/TP一般也比非养殖海区站位高。 夏季，胶州湾菲律宾蛤仔养殖区（平均密度按600ind/m2计算）单位面积（m2）的生物沉积速率平均为176g/m2•d，对于整个海湾，将有1.2万吨的悬浮颗粒物通过贝类排粪作用沉积到海底。通过菲律宾蛤仔的呼吸作用，单位面积（m2）的耗氧量为6.67gO2/m2•d；同时从海底向水体释放大约为16.7μmolN /m2•d和3.3μmolP/m2•d的溶解态N和P；对于整个海湾，菲律宾蛤仔将耗掉水体中的467T/d氧气，同时向水体中释放16.4T/d氨氮和7.2T/d无机磷。对于半封闭的胶州湾，大规模菲律宾蛤仔的滩涂养殖其产生的生物沉积物聚集于海底可能会对海区底部的物理化学和生物环境产生很大影响；而氧的消耗、氨和无机磷的排泄，会对水层中的物理化学环境和营养盐循环产生很大影响。从而由此推测，菲律宾蛤仔高密度大规模的养殖，在整个胶州湾水层－底栖系统耦合作用中可能起着很重要的作用。

雨生红球藻诱变育种及突变株的筛选

虾青素是良好的着色剂，具有超强的抗氧化、捕获自由基、抗癌变，抗紫外线辐射、增强免疫等功能，在医疗、水产、食品和化妆品等生产领域有着广泛的市场。雨生红球藻（Haematococcus pluvialis）是一种单细胞的绿藻，在逆境条件下可积累高达4%干重的虾青素，是目前已知的天然虾青素含量最高的生物资源，规模化培养雨生红球藻是获取天然虾青素的最佳途径。 目前实验室研究和规模化培养使用的藻株多为出发株，存在一些缺陷：养殖过程中易被其它微藻、变形虫、原生动物、细菌等污染；只适合低温培养，28℃以上生长受抑制；生长相对较慢，培养周期长；尽管雨生红球藻是已知的天然虾青素含量最高的生物资源，但其含量仍然需要提高以适合规模化生产。因此筛选出温度适应范围广、生长速率快、虾青素含量高的雨生红球藻株一直是努力的方向。 本研究选用化学诱变剂甲基磺酸乙酯（EMS）和叠氮钠(NaN3)以及物理诱变剂紫外线（UV)分别处理雨生红球藻，测定各种诱变剂的致死率，及对红球藻生长和虾青素积累的影响，根据实验结果确定合适的诱变剂量和诱变时间，通过初筛、复筛、再次诱变等分离、筛选出生长速度快、虾青素含量高的单一藻株并进一步利用高压液相色谱等方法进行鉴定。 实验结果表明：浓度为0.2％-1.0％EMS处理4h，雨生红球藻H2的死亡率7%-35%；紫外线照射2min-10min，雨生红球藻H0的死亡率10%-98％；叠氮钠30mg/L－270 mg/L处理24h，雨生红球藻H0的死亡率为20％-100％。各种诱变剂诱变结束后立即观察发现死亡细胞的形态相似但存活细胞相态有所不同，死亡细胞呈浅绿色，轮廓模糊，形状不规则，质壁间隙消失，胞内有白色空泡；EMS处理后存活细胞，呈圆球状，部分失去鞭毛和前端突起，游动缓慢，处理后第二天，细胞即恢复游动和分裂，分裂方式与正常细胞相似。紫外线和叠氮钠处理后存活细胞壁加厚，呈深绿色，圆球状，部分失去鞭毛和突起，严重抑制细胞的游动和分裂，出现不动细胞，培养一段时间后才出现分裂细胞，游动的和不动的存活细胞主要是采用不对称无性繁殖，这与正常细胞的不对称无性繁殖有所不同，正常细胞中不对称无性繁殖的母细胞多为不动细胞。紫外线和叠氮钠处理后部分细胞还产生大量的小孢子（配子？），直径较小1-4m，在母细胞内不停摆动，四、五天后逐渐脱离母细胞。这和以前描述的有性繁殖很相似，但由于没观察到后期孢子配对过程和缺乏小孢子的DNA含量和倍性的有关数据，不能确定观察到的小孢子现象为雨生红球藻的有性繁殖。 各种诱变剂处理后，各组存活藻株的生长和虾青素积累情况同对照组相比发生了变化：EMS实验中0.4％EMS组生长速率提高24％，其它组的细胞生长速率有所降低。各组单细胞虾青素含量和积累速率均高于对照组，其中0.4％和0.8％处理组单细胞内虾青素含量分别提高了76％和90％，积累速率分别提高了69％和115％；紫外线实验中4min、6min和10min组生长速率分别提高20％、17％和35%，各组藻细胞虾青素积累速率有不同程度的提高，其中2min组和10min组虾青素积累速率提高幅度最大分别为32%和31%，单细胞内虾青素含量除10min处理组提高48%，其它各组均有不同幅度的降低；叠氮钠实验中60mg/L、120mg/L和180mg/L组细胞生长速率分别提高了13％、20％和9％，各组藻细胞内虾青素含量和虾青素积累速率得到明显的提高。其中60 mg/L和180 mg/L组单细胞内虾青素含量提高幅度最大，分别提高75%和140%。180mg/L和240mg/L组虾青素积累速率提高幅度最大，分别为280％和250％。 诱变后通过固体平板分离，得到一系列单一藻株，其中0.4%浓度EMS处理雨生红球藻H2 4h后分离到H2-419在生物量上比H2高出20％,但虾青素含量提高幅度不大。用UV对突变株H2-419再次诱变得到较稳定的藻株H2-419-4，培养五个月后与H2相比生物总量提高68%，单细胞内虾青素含量提高28％，经高压液相色谱分析证明H2-419-4类胡萝卜素提取物中主要成分仍然为虾青素，和出发株H2相比色素成分没有发生变化，只是各成分比例有所不同。 还分析研究了其它单一藻株，但或是稳定性较差，或虾青素含量高而生长慢。如雨生红球藻H2经0.02％EMS诱变十天，分离得到的E-L-22和出发株相比虾青素产量提高40％，但生物总量上降低了27％；5min紫外线照射雨生红球藻H0后，分离得到的UV-5-16和UV-5-14在生物总量分别提高7％和36％。叠氮钠200mg/L处理24h分离得到的藻株N-2-42生物总量提高43％，但藻株N-2-43生物总量比出发株降低8％。

海带蓝光受体及相关基因的研究

本研究中运用四种不用的方法提取海带孢子体RNA，通过对比分析，确定了采用CTAB提取液[2% (w/v) CTAB,100 mM Tris-HC1 (pH 8.0), 50 mM EDTA, pH 7.5, 2 M NaCl, 50 mM DTT]提取RNA的方法，该方法的主要改进之处： (1) 高浓度（50 mM）的 DTT作为防止多酚氧化的还原剂加入提取液中；(2) 1/4 体积的乙醇和 1/9 体积的3 M 醋酸钾（pH 4.8）用来沉淀去除多糖；(3) 1/10倍体积的3 mol/L醋酸钠（pH 5.0）和两倍体积的乙醇选择性沉淀RNA；(4) -80℃沉淀30 min沉淀RNA。CTAB改进法提取的海带孢子体RNA质量好（A260/280 =1.96±0.05)，产量高（68 μg/g），分离的RNA可用于RT-PCR反应，扩增相关基因片段。该方法提取海带配子体RNA效果也很好。 根据拟南芥(Arabidopsis thaliana)、 莱茵衣藻(Chlamydomonas reinhardtii) 、铁线蕨(Adiantum capillus-veneris)、转板藻(Mougeotia scalaris)和无隔藻(Vaucheria frigida)等的蓝光受体蛋白保守序列，设计一系列简并引物，进行RT-PCR扩增，筛选并克隆测序了4条序列片段，通过比对分析，所得序列与已知的蓝光受体基因序列不一致。在所获得的序列中，一条783 bp的序列与肌醇-1-磷酸合成酶基因的片段有较高的相似性，通过RACE法克隆出该基因的3’末端，5’末端克隆没有成功，全长序列有待深入分析。