Cys mutants in functional regions of Sticholysin I clarify the participation of these residues in pore formation

Experimental evidence shows that the mechanism of pore formation by actinoporins is a multistep process, involving binding of the water-soluble monomer to the membrane and subsequent oligomerization on the membrane surface, leading to the formation of a functional pore. However, as for other eukaryotic pore-forming toxins, the molecular details of the mechanism of membrane insertion and oligomerization are not clear. In order to obtain further insight with regard to the structure-function relationship in sticholysins, we designed and produced three cysteine mutants of recombinant sticholysin I (rStI) in relevant functional regions for membrane interaction: StI E2C and StI F15C (in the N-terminal region) and StI R52C (in the membrane binding site). The conformational characterization derived from fluorescence and CD spectroscopic studies of StI E2C, StI F15C and StI R52C suggests that replacement of these residues by Cys in rStI did not noticeably change the conformation of the protein. The substitution by Cys of Arg(52) in the phosphocholine-binding site, provoked noticeable changes in rStI permeabilizing activity; however, the substitutions in the N-terminal region (Glu(2), Phe(15)) did not modify the toxin`s permeabilizing ability. The presence of a dimerized population stabilized by a disulfide bond in the StI E2C mutant showed higher pore-forming activity than when the protein is in the monomeric state, suggesting that sticholysins pre-ensembled at the N-terminal region could facilitate pore formation. (C) 2011 Elsevier Ltd. All rights reserved.

Mechanistic study of the addition reaction of TeCl(4) to alkynes: Participation of TeCl(3) centered-radical

The mechanism of the addition reaction of TeCl(4) to alkynes was indirectly established by the detection of TeCl(3) centered radicals using EPR spin trapping, ESI-MS and ESI-MS/MS characterization. Crown Copyright (C) 2008 Published by Elsevier B. V. All rights reserved.

Citizens and Contemporary Science Ways to dialogue in science centre contexts.

The current paper presents a study conducted at At-Bristol Science Centre, UK. It is a front-end evaluation for the “Live Science Zone” at At-Bristol, which will be built during the autumn of 2004. It will provide a facility for programmed events and shows, non-programmed investigative activities and the choice of passive or active exploration of current scientific topics. The main aim of the study is to determine characteristics of what kind of techniques to use in the Live Science Zone. The objectives are to explore what has already been done at At-Bristol, and what has been done at other science centres, and to identify successful devices. The secondary aim is mapping what sorts of topics that visitors are actually interested in debating. The methods used in the study are deep qualitative interviews with professionals working within the field of science communication in Europe and North America, and questionnaires answered by visitors to At-Bristol. The results show that there are some gaps between the intentions of the professionals and the opinions of the visitors, in terms of opportunities and willingness for dialogue in science centre activities. The most popular issue was Future and the most popular device was Film.

A comparative study of immigrants’ political participation in Sweden and the Netherlands

This study deals with immigrants’ political participation in Sweden and the Netherlands. Scholars have recognized low level of political participation of immigrants in Sweden compared to the Netherlands. The main goal of this study is to analyze the institutional influence, mainly from political parties over immigrants’ motivation for active electoral participation. The modified actor-context model uses here as the main theoretical framework. In addition, social capital theory employs to analyze immigrants’ voluntary organizational membership. This study confirms that, Swedish immigrants have the lower participation rate in the political sphere, at lest to a certain extent, than its counterparts the Dutch immigrants. This study also confirms the argument that contextual factors can influence actor’s motivations in integration-oriented action, and similarly it validates the necessity of enlargement of the actor-context model.

Muslims' participation in Ethiopian Civil Society: findings from field research in Addis Ababa

This thesis is an investigation into the Ethiopian Civil Society, with a focus on Muslims’participation and activities. This research is the result of a series of interviews carried on in AddisAbaba during my staying there thank to a scholarship from Pavia University.Chapter One is a general introduction of the study, presenting the object, the methodology anduse of sources as well as the state of the current research of the topics covered by this research.Chapter Two is a framework chapter about Islām in Ethiopia offering an historical perspective aswell as focusing on its characteristics and current developments. Chapter Three deals withEthiopian Civil Society characteristics and with its legal framework. Chapter Four constitutes thecore of this research: in it, I collected the findings of my research describing the presence ofMuslims into Ethiopian Civil Society. I analyzed the activities and characteristics of the differentorganizations and associations that I met in Addis Ababa, their self-representation concerningtheir being related with Islām and their opinions on Muslims’ marginalization and lack of nonpoliticizationin Ethiopia. A set of conclusions constitutes the last section of the thesis.

Patient participation : what it is and what it is not

In general, patient participation is regarded as being informed and partaking in decision making regarding one’s care and treatment. This interpretation is common in legislation throughout the Western world and corresponding documents guiding health care professionals, as well as in scientific studies. Even though this understanding of the word participation can be traced to a growing emphasis on individuals’ autonomy in society and to certain dictionary defi nitions, there are other ways of understanding participation from a semantic point of view, and no trace of patients’ descriptions of what it is to participate can be found in these definitions. Hence, the aim of this dissertation was to understand patients’ experience of the phenomenon of patient participation. An additional aim was to understand patients’ experience of non-participation and to describe the conditions for patient participation and non-participation, in order to understand the prerequisites for patient participation. The dissertation comprises four papers. The philosophical ideas of Ricoeur provided a basis for the studies: how communication can present ways to understand and explain experiences of phenomena through phenomenological hermeneutics. The first and second studies involved a group of patients living with chronic heart failure. For the fi rst study, 10 patients were interviewed, with a narrative approach, about their experience of participation and non-participation, as defi ned by the participants. For the second study, 11 visits by three patients at a nurse-led outpatient clinic were observed, and consecutive interviews were performed with the patients and the nurses, investigating what they experience as patient participation and non-participation. A triangulation of data was performed to analyse the occurrence of the phenomena in the observed visits. For paper 3 and 4, a questionnaire was developed and distributed among a diverse group of people who had recent experience of being patients. The questionnaire comprised respondent’s description of what patient participation is, using items based on findings in Study 1, along with open-ended questions for additional aspects and general issues regarding situations in which the respondent had experienced patient participation and/or non-participation. The findings show additional aspects to patient participation: patient participation is being provided with information and knowledge in order for one to comprehend one’s body, disease, and treatment and to be able to take self-care actions based on the context and one’s values. Participation was also found to include providing the information and knowledge one has about the experience of illness and symptoms and of one’s situation. Participation occurs when being listened to and being recognised as an individual and a partner in the health care team. Non-participation, on the other hand, occurs when one is regarded as a symptom, a problem to be solved. To avoid non-participation, the information provided needs to be based on the individual’s need and with recognition of the patient’s knowledge and context. In conclusion, patient participation needs to be reconsidered in health care regulations and in clinical settings: patients’ defi nitions of participation, found to be close to the dictionaries’ description of sharing, should be recognised and opportunities provided for sharing knowledge and experience in two-way-communication.

Enforcement in environmental law: an economic analysis of citizen suits

Previous to 1970, state and federal agencies held exclusive enforcement responsibilities over the violation of pollution control standards. However, recognizing that the government had neither the time nor resources to provide full enforcement, Congress created citizen suits. Citizen suits, first amended to the Clean Air Act in 1970, authorize citizens to act as private attorney generals and to sue polluters for violating the terms of their operating permits. Since that time, Congress has included citizen suits in 13 other federal statutes. The citizen suit phenomenon is sufficiently new that little is known about it. However, we do know that citizen suits have increased rapidly since the early 1980's. Between 1982 and 1986 the number of citizen suits jumped from 41 to 266. Obviously, they are becoming a widely used method of enforcing the environmental statutes. This paper will provide a detailed description, analysis and evaluation of citizen suits. It will begin with an introduction and will then move on to provide some historic and descriptive background on such issues as how citizen suit powers are delegated, what limitations are placed on the citizens, what parties are on each side of the suit, what citizens can enforce against, and the types of remedies available. The following section of the paper will provide an economic analysis of citizen suits. It will begin with a discussion of non-profit organizations, especially non-profit environmental organizations, detailing the economic factors which instigate their creation and activities. Three models will be developed to investigate the evolution and effects of citizen suits. The first model will provide an analysis of the demand for citizen suits from the point of view of a potential litigator showing how varying remedies, limitations and reimbursement procedures can effect both the level and types of activities undertaken. The second model shows how firm behavior could be expected to respond to citizen suits. Finally, a third model will look specifically at the issue of efficiency to determine whether the introduction of citizen enforcement leads to greater or lesser economic efficiency in pollution control. The database on which the analysis rests consists of 1205 cases compiled by the author. For the purposes of this project this list of citizen suit cases and their attributes were computerized and used to test a series of hypotheses derived from three original economic models. The database includes information regarding plaintiffs, defendants date notice and/or complaint was filed and statutes involved in the claim. The analysis focuses on six federal environmental statutes (Clean Water Act} Resource Conservation and Recovery Act, Comprehensive Environmental Response Compensation and Liability Act, Clean Air Act, Toxic Substances Control Act, and Safe Drinking Water Act) because the majority of citizen suits have occurred under these statutes.

A Serious Gaming Approach For Serious Stakeholder Participation

Serious games are a category of games which are designed for a specific purpose other than for pure entertainment. It is not a new concept but serious games using real data, coupled with real time modelling and combining model results with social and economic factors opens up a new paradigm for active stakeholder participation. DHI and UNEP-DHI Centre initiated a project called Aqua Republica where a virtual world is developed which allows participants to develop a river basin and visualise the consequences of their decisions. The aim of this project is to raise awareness of the interconnectivity of water and educate on integrated water resources management. Aqua Republica combines a game layer with a water allocation model, MIKE BASIN, to create an interactive, realistic virtual environment where players play the role of a catchment manager of an undeveloped river catchment. Their main objective is to develop the river catchment to be as prosperous as it can be. To achieve that, they will need to generate a good economy in the catchment to provide the funds needed for development, have a steady food supply for their population and enough energy and water for the catchment. Through these actions by the player, a meaningful play is established to engage players and to educate them about the complex relationships between developmental actions in a river basin and the natural environment as well as their consequences. The game layer also consists of a reward system to encourage learning. People can play and replay the game, get rewarded from performing the right principles and penalised from failures in the game. This abstract will explain the concept of the game and how it has been used in a stakeholder participation environment.

Participação Infantil: experiência de pesquisa realizada em organização social na Zona Oeste do Rio de Janeiro

Este trabalho se propõe a ser um fio na teia de reflexões que tecem o debate sobre a participação infantil a partir do ponto de vista das crianças em co-operação com adultos. Sua trama se compõe de campos distintos e autônomos - infância, direito social e participação -, que se cruzam e inter-relacionam, investigados no âmbito de uma experiência realizada na organização social Fundação Xuxa Meneghel, que tem como eixo central de suas ações a promoção e a proteção de crianças e adolescentes que vivem em situação de vulnerabilidade social na Zona Oeste do município do Rio de Janeiro. Trata-se de uma micro-experiência, que pretende compreender o ser criança em relação aos seus direitos humanos e sociais, observando sua influência nas práticas sociais presentes na nossa cultura. O direito à participação infantil - entendida como ferramenta para a auto-proteção e a promoção das crianças como sujeitos plenos exercitando a cidadania - se materializou numa investigação em que as concepções metodológicas convencionais foram se re-construindo por meio das vozes infantis em diálogo com seus pares e com os adultos. As crianças apresentaram seus pontos de vista e as significações que atribuem ao seu entorno e a suas possibilidades e limites de participar de forma genuína nas suas principais instâncias de socialização: família, escola e comunidade. A experiência se constitui numa provocação do olhar para as identidades e as culturas das crianças que dela participaram, para suas vivências expressas por representações de ações e emoções contextualizadas no tempo e no espaço, numa tentativa de compor com elas - e ampliando para todas as crianças da instituição - uma compreensão do que significa o direito de participar, ensinado e apreendido em perspectiva social e cultural.

Desenvolvimento local, cidadania e arranjos produtivos locais: um estudo no estado do Rio de Janeiro

The aim of the present study was to verify how the participation and interaction of the local government public and private instittutions and civil society, might promote sustainable development, taking as a reference the Local Productive Arrangements (LPA) of Nova Friburgo and its Region. This work was conducted after a theoretical discussion concerning local development, citizenship, local development with citizenship and local productive arrangements. Afterwards, semi-structured interviews were realized with 32 citizens from five cities that were part of the Fashion Development Council, using the discourse analysis to data treatment. It was verified that the productive arrangement in the Centro-Norte Fluminense region of the Rio de Janeiro State has one of its main competitive advantages the pool of institutions, that articulate and support the underwear fashion sector. However, the APL presents many other characteristics making impossible to classify it as a cluster or an industrial district since there is still a need for cooperation among institutions and undertakers. In addition, there is no integration between the government instances and civil society in relation to the LPA development. So, it is concluded that there is a need for more union and dialogue among all of these involved actors, to overcome ¿bottle-necks¿ in the sector of underwear fashion in the region, such as informality of a huge number of companies and the low qualification of employees and undertakers.

Administração pública federal: propostas para o fortalecimento do controle sobre os atos praticados e o respectivo combate à corrupção

The characteristics of the Brazilian historic context, under which the predominant social relations have developed, have led to a process of income concentration and to the political power of the dominant classes. The slavery abolishment hasn't guaranteed the people the rights secured to privileged citizens in general. Such practices were observed during historic process as the low level literacy shown by the census of 1920, the political domination of oligarchies and the military coup, all as determining factors in process of political power concentration. The social indicators and the corruption are extremely unfavorable to our country, but we wonder if that happens only in underdeveloped societies. It is possible that even the American society, even the most developed societies, under the democratic capitalism, can suffer negative consequences of some corruption in the capitalism system. Our observations have led to the perception that all democratic society must be regulated by the State in order to preserve the stability of the system. It has also been observed that it is necessary more effective popular participation in order to neutralize economic groups¿ pressure. It has also become evident the necessity of reduction of commissioned office in the federal public administration. And, finally, it is fundamental to propose an amendment to the construction that allows the Public Ministry to have access to any bank, fiscal or telephonic information of anyone that is in office: It should be called "The Law of Moral Transparency". Those proposals will only be possible if there is massive popular participation and we hope that they express our people¿s will in order to appose to those who act only to obtain private benefits.

Institucionalização de práticas participativas na administração pública: caminho para democratização do poder local

This dissertation discuss the institutionalization of citizen participation in local political administration and the democratization of this administration. We take as the object of our study Niterói OP" (sharing? budget). We study the citizens projects and its implementation., focusing our attention on personal conflicts and power disputs. Our work is based on the following concepts: democracy, descentralization, citizenship, local political power and social? capital?. We verify the different procedures used by Niterói "OP" coordination in order to integrate the citizens in this process. The main concern of our work is the degree of citizen participation in local political power, therefore, we shall not discuss in details the budget itself."

Os tribunais de contas e o controle social : a proposta de criação de uma ouvidoria para o Tribunal de Contas do Estado do Rio de Janeiro e sua importância no processo democrático fluminense

The aim of this dissertation is to study the contribution given by the Brazilian court of accounts, with the creation of the ombudsman on those institutions, to the exercise of the social control made by its citizens. Being considered one of the key inventions in the field of the external control of the public management, the ombudsman of the court of accounts is the most important means of control the society may use over the public management, as well as the place where the society and court of accounts might debate and get to an agreement concerning on how to spend the public asset in a way that will benefit the citizens. In order to fulfill the aim of the dissertation, the concepts of citizenship, particularly the deliberative citizenship, were recaptured, as well as the means of control in the public management and the role of the court of accounts as a participant in the external control of the public accounts. Lastly, some of the 18 ombudsman linked to Brazilian courts of account are presented in the dissertation. The Ombudsman of the Courts of Accounts of the states of Paraná and Pernambuco are emphasized once it is understood that they are in the path of transcending the concept of popular participation to the popular sovereignty, in which the debate with the society might, in a near future, decide possibly the course of the audits of those entities.

Orçamento participativo e a formação para a cidadania

Nossa pesquisa pretende descrever a trajetória do Orçamento Participativo desde seu surgimento, em 1988, até 2000, através de seu processo de implantação e desenvolvimento que vem garantindo uma formação para a cidadania a partir de uma prática de participação política junto às populações integrantes do espaço social de Porto Alegre. Esse sistema estratégico de administração dos recursos públicos constituiu-se num exemplo da participação popular no espaço social. Tomou-se mesmo um instrumento símbolo da cidade, pois suas técnicas contribuem para esboçar um novo perfil da "formação para a cidadania". Nossa metodologia, partindo do objetivo de investigar a natureza da formação para a cidadania proporcionado pelo Orçamento Participativo, utilizou como técnicas de procedimento pesquisas documentais e bibliográficas, além de contatos diretos com vários agentes. Os instrumentos de pesquisa empregados nas coletas de dados, análise e interpretação, seguiram as seguintes técnicas: entrevistas junto aos conselheiros populares regionais e aos delegados das comissões temáticas.(representantes eleitos no processo do Orçamento Participativo); pesquisa documental e análise de conteúdo. As categorias de análise trabalhadas foram: o Orçamento Participativo, a formação para a cidadania e o habitus político (como um conhecimento adquirido, um haver, e/ou capital que indicam uma disposição incorporada, quase postural). Tal pesquisa é inédita devido à utilização do pressuposto teóricometodológico do sociólogo francês Pierre Bourdieu. Seus resultados têm demonstrado que garantir, em nível municipal, a transferência do poder de decisão dos investimentos públicos aos cidadãos de Porto Alegre proporciona um processo de politização cada vez mais amplo através da participação, autonomia e conseqüente formação de um novo habitus, rumo à auto gestão. Considerando que o processo de formação oportunizado pelo Orçamento Participativo garante uma valorização dos agentes sociais, este desencadeia um desabrochar do cidadão. A formação para a cidadania portanto, proporcionada por essa prática em mais de dez anos consecutivos, vem obtendo um reconhecimento internacional de destaque no cenário político do final do século xx.