Sexual transmission of human T-cell lymphotropic virus type 1

Human T-cell lymphotropic virus type 1 (HTLV-1) is endemic in many parts of the world and is primarily transmitted through sexual intercourse or from mother to child. Sexual transmission occurs more efficiently from men to women than women to men and might be enhanced by sexually transmitted diseases that cause ulcers and result in mucosal ruptures, such as syphilis, herpes simplex type 2 (HSV-2), and chancroid. Other sexually transmitted diseases might result in the recruitment of inflammatory cells and could increase the risk of HTLV-1 acquisition and transmission. Additionally, factors that are associated with higher transmission risks include the presence of antibodies against the viral oncoprotein Tax (anti-Tax), a higher proviral load in peripheral blood lymphocytes, and increased cervicovaginal or seminal secretions. Seminal fluid has been reported to increase HTLV replication and transmission, whereas male circumcision and neutralizing antibodies might have a protective effect. Recently, free virions were discovered in plasma, which reveals a possible new mode of HTLV replication. It is unclear how this discovery might affect the routes of HTLV transmission, particularly sexual transmission, because HTLV transmission rates are significantly higher from men to women than women to men.

Invasive aspergillosis in a user of inhaled cocaine: rhinosinusitis with bone and cartilage destruction

Aspergillosis is an infection caused by saprophytic fungi of the genus Aspergillus, which typically occurs in immunosuppressed individuals, but has also been reported in immunocompetent patients. The main routes of entry are the respiratory tract, skin, cornea, and ear, and the infection may be localized or disseminated by contiguity or vascular invasion. We report a severe case of rhinosinusitis with cutaneous involvement, caused by invasive aspergillosis, in an immunocompetent user of inhaled cocaine. Invasive aspergillosis related to cocaine abuse has not yet been reported in the literature. After itraconazole treatment and surgical debridement, complete clinical remission was achieved. Nasal reconstruction with a skin graft over a silicone prosthesis resulted in a satisfactory esthetic outcome.

Toxoplasma gondii infection: seroprevalence and associated risk factors among primary schoolchildren in Lagos City, Southern Nigeria

INTRODUCTION: Toxoplasma gondii infection has been described as the most widespread zoonotic infection of humans and other animals. Information concerning T. gondii infection among schoolchildren is unavailable in Lagos City, Nigeria. METHODS: This cross-sectional study investigated the seroprevalence and risk factors associated with T. gondii infection among primary schoolchildren (PSC) from a community located in the center of Lagos, southern Nigeria, from November 2013 to March 2014. A total of 382 PSC were screened for the presence of sera anti-T. gondii antibodies using a latex agglutination test (TOXO Test-MT, Tokyo, Japan). A cutoff titer of ≥ 1:32 was considered positive, while titers ≥ 1:1,024 indicated high responders. Questionnaires were also used to obtain data on possible risk factors from parents/guardians. RESULTS: The overall seroprevalence was 24% (91/382), and 83.5% (76/91) of seropositive PSC were classified as high responders. Among the risk factors tested, including contact with cats and soil, consumption of raw meat and vegetables, and drinking unboiled water, none showed statistical significance after multivariate adjustment. No associations were observed among age, gender, body mass index (BMI), and parents' occupation/educational level. CONCLUSIONS: The findings in this study show evidence of active infection, and hence, there is need for urgent preventive measures in this city. Further investigation is required to clarify the transmission routes. Policy makers also need to initiate prevention and control programs to protect pregnant women and immunocompromised patients in particular because they are more severely affected by T. gondii infection.

Chlamydia trachomatis serotype A infections in the Amazon region of Brazil: prevalence, entry and dissemination

INTRODUCTION: Chlamydia infection is associated with debilitating human diseases including trachoma, pneumonia, coronary heart disease and urogenital diseases. Serotypes of C. trachomatis show a fair correlation with the group of diseases they cause, and their distribution follows a well-described geographic pattern. Serotype A, a trachoma-associated strain, is known for its limited dissemination in the Middle East and Northern Africa. However, knowledge on the spread of bacteria from the genus Chlamydia as well as the distribution of serotypes in Brazil is quite limited. METHODS: Blood samples of 1,710 individuals from ten human population groups in the Amazon region of Brazil were examined for antibodies to Chlamydia using indirect immunofluorescence and microimmunofluorescence assays. RESULTS: The prevalence of antibodies to Chlamydia ranged from 23.9% (Wayana-Apalai) to 90.7% (Awa-Guaja) with a mean prevalence of 50.2%. Seroreactivity was detected to C. pneumoniae and to all serotypes of C. trachomatis tested; furthermore, we report clear evidence of the as-yet-undescribed occurrence of serotype A of C. trachomatis. CONCLUSIONS: Specific seroreactivity not only accounts for the large extent of dissemination of C. trachomatis in the Amazon region of Brazil but also shows an expanded area of occurrence of serotype A outside the epidemiological settings previously described. Furthermore, these data suggest possible routes of Chlamydia introduction into the Amazon region from the massive human migration that occurred during the 1,700s.

First report of schistosomiasis on Serrambi beach, Ipojuca, State of Pernambuco

Abstract: INTRODUCTION : The expansion of schistosomiasis to previously unaffected areas is being monitored by identifying new cases and georeferencing outbreaks of vector snails. METHODS : In 2014, the Laboratório de Esquistossomose began an epidemiological survey in Serrambi and registered 2,574 people living there. RESULTS : Of these subjects, 1,414 (54.9%) underwent feces examination and 63 (4.5%) were diagnosed with Schistosoma mansoni infection. At this locality, seven breeding sites each were identified for Biomphalaria straminea and Biomphalaria glabrata. At two sites, B. glabrata were shedding cercariae. CONCLUSIONS : Implementing preventive measures is necessary to avoid the establishment of schistosomiasis in yet another tourist locality, Pernambuco.

Musées et casinos dans une ville "patrimoine mondial": authenticité et hyperréalité, deux formes culturelles de l'espace urbain de Macao

Texto submetido para publicação em 2011.

A maritimidade portuguesa : a extensão da plataforma continental

As potencialidades da plataforma continental estendida são enormes e variadas, desde as que podem ser obtidas através de sectores tradicionais – como portos e marinas ou turismo náutico – até as que advirão de novos sectores como a exploração dos fundos do mar ou a energia das ondas, entre outras. Com efeito, devido ao alargamento resultante das negociações nas Nações Unidas, é praticamente garantido que Portugal passe a controlar um espaço marítimo, acrescido de 2.1 milhões de km2, isto é, vinte vezes a extensão da sua superfície terrestre. A maritimidade de Portugal constitui um traço profundamente marcado na sua história, bem como nas suas tradicionais práticas económicas, sociais e simbólicas. Na verdade, os últimos anos testemunharam a criação de um amplo consenso quanto ao papel determinante que o desenvolvimento costeiro, a superfície e o comprimento dos limites do “Mar Português” terá no futuro do país. Da articulação entre o Território Nacional, o Oceano Atlântico e o Continente Europeu, associada à confluência das vias de comunicação marítimas que ligam a Europa à América do Norte e do Sul configurar-e-ão os aspectos essenciais do designado “Espaço Estratégico de Interesse Nacional Permanente”. A área geográfica prioritária para o desenvolvimento das acções militares de Defesa Nacional e dos interesses vitais permanentes inclui assim – para além do Território Nacional – o Espaço Interterritorial, o Mar Territorial (MT), o espaço aéreo sob responsabilidade nacional e a Zona Económica Exclusiva (ZEE). O País é titular de soberania no território continental e nos arquipélagos da Madeira dos Açores, para além de estar comprometido com alguns Estados da CPLP, que têm no Atlântico Sul uma importante e poderosa presença. Pela geografia e pela definição dos interesses dos Estados e dos grandes espaços em que estão incluídos, Portugal está na articulação da segurança do Atlântico Norte com a segurança do Atlântico Sul. Esta realidade acarreta novos desafios para a Defesa Nacional, assumindo como preocupações acrescidas o terrorismo, o tráfico e a pirataria marítimas, entre outras.

Engineering bio-based polymers using alternative solvents and processes

The work presented in this thesis explores novel routes for the processing of bio-based polymers, developing a sustainable approach based on the use of alternative solvents such as supercritical carbon dioxide (scCO2), ionic liquids (ILs) and deep eutectic solvents (DES). The feasibility to produce polymeric foams via supercritical fluid (SCF) foaming, combined with these solvents was assessed, in order to replace conventional foaming techniques that use toxic and harmful solvents. A polymer processing methodology is presented, based on SCF foaming and using scCO2 as a foaming agent. The SCF foaming of different starch based polymeric blends was performed, namely starch/poly(lactic acid) (SPLA) and starch/poly(ε-caprolactone) (SPCL). The foaming process is based on the fact that CO2 molecules can dissolve in the polymer, changing their mechanical properties and after suitable depressurization, are able to create a foamed (porous) material. In these polymer blends, CO2 presents limited solubility and in order to enhance the foaming effect, two different imidazolium based ILs (IBILs) were combined with this process, by doping the blends with IL. The use of ILs proved useful and improved the foaming effect in these starch-based polymer blends. Infrared spectroscopy (FTIR-ATR) proved the existence of interactions between the polymer blend SPLA and ILs, which in turn diminish the forces that hold the polymeric structure. This is directly related with the ability of ILs to dissolve more CO2. This is also clear from the sorption experiments results, where the obtained apparent sorption coefficients in presence of IL are higher compared to the ones of the blend SPLA without IL. The doping of SPCL with ILs was also performed. The foaming of the blend was achieved and resulted in porous materials with conductivity values close to the ones of pure ILs. This can open doors to applications as self-supported conductive materials. A different type of solvents were also used in the previously presented processing method. If different applications of the bio-based polymers are envisaged, replacing ILs must be considered, especially due to the poor sustainability of some ILs and the fact that there is not a well-established toxicity profile. In this work natural DES – NADES – were the solvents of choice. They present some advantages relatively to ILs since they are easy to produce, cheaper, biodegradable and often biocompatible, mainly due to the fact that they are composed of primary metabolites such as sugars, carboxylic acids and amino-acids. NADES were prepared and their physicochemical properties were assessed, namely the thermal behavior, conductivity, density, viscosity and polarity. With this study, it became clear that these properties can vary with the composition of NADES, as well as with their initial water content. The use of NADES in the SCF foaming of SPCL, acting as foaming agent, was also performed and proved successful. The SPCL structure obtained after SCF foaming presented enhanced characteristics (such as porosity) when compared with the ones obtained using ILs as foaming enhancers. DES constituted by therapeutic compounds (THEDES) were also prepared. The combination of choline chloride-mandelic acid, and menthol-ibuprofen, resulted in THEDES with thermal behavior very distinct from the one of their components. The foaming of SPCL with THEDES was successful, and the impregnation of THEDES in SPCL matrices via SCF foaming was successful, and a controlled release system was obtained in the case of menthol-ibuprofen THEDES.

Depois de Orpheu : 1916-1935

O objetivo deste trabalho era verificar como, nos jornais e revistas, o Primeiro Modernismo tinha sido acolhido. Depois do escândalo de Orpheu e da forma agressiva como os seus colaboradores tinham sido recebidos, cabia verificar se tinha havido, ou não, uma evolução na análise da sua obra ao longo dos anos, de 1916 a 1935, tanto mais que as vidas de cada um destes tinha evoluído de forma diferenciada. As mortes prematuras de uns, a subida a postos de influência e poder, ou a longevidade de outros foram fatores que poderiam ter condicionado a evolução da crítica jornalística. Por um lado, os críticos poderiam ter alterado a sua visão face a estes criadores, podendo o tempo ter funcionado a favor destes. O que em 1915 era estranho e bizarro poderia em 1935 ser inédito e original. Por outro lado, a conjuntura política poderia condicionar não só a criação artística, mas também o seu enquadramento social. Em 1915, a guerra entre monárquicos e republicanos foi marcada pelas referências a Orpheu. Mais tarde com a ditadura militar e a ascensão do Estado Novo poderão os nossos artistas voltar a ser alvo de referências políticas. A verdade é que tal não acontece. O poder político menospreza-os, apesar de nalguns casos terem uma participação ativa nas suas fileiras. A verdade é que os autores mais importantes e significativos foram, pouco a pouco, e com o passar dos anos, integrados na sociedade literária e começaram a ser vistos como criadores a ter em conta, senão mesmo de referência. O seu aparecimento em jornais e revistas na qualidade de escritores, jornalistas, artistas plásticos ou mesmo críticos fez com que estivessem sob os holofotes da imprensa e como tal sujeitos a uma análise permanente. E esta foi-se alterando com o passar do tempo e com o reconhecimento dos mesmos. Hoje ninguém duvida do seu valor, mas entre 1915 (data da publicação de Orpheu e 1935 (ano da morte de Pessoa) todos eram vistos como loucos, jovens com alguma habilidade para a escrita, promessas do panorama literário e finalmente, em alguns casos, motivos de orgulho para o nosso património artístico. Só uma leitura dos periódicos mais relevantes permitiria estudar a receção do nosso Primeiro Modernismo. E foi esse o objetivo desta tese.

Electric vehicle assistant based in driver profile

This paper presents the outcomes of a research work consisting in the development of an Electric Vehicle Assistant (EVA), which creates and stores a driver profile where are contained the driving behaviours related with the EV energy consumption, the EV battery charging information, and the performed routes. This is an application for mobile devices that is able to passively track the driver behaviour and to access several information related with the EV in real time. It is also proposed a range prediction approach based on probability to take into account unpredictable effects of personal driving style, traffic or weather.

Non emergency patients transport - a mixed integer linear programming

This work presents a model and a heuristic to solve the non-emergency patients transport (NEPT) service issues given the new rules recently established in Portugal. The model follows the same principle of the Team Orienteering Problem by selecting the patients to be included in the routes attending the maximum reduction in costs when compared with individual transportation. This model establishes the best sets of patients to be transported together. The model was implemented in AMPL and a compact formulation was solved using NEOS Server. A heuristic procedure based on iteratively solving problems with one vehicle was presented, and this heuristic provides good results in terms of accuracy and computation time.

The non-emergency patient transport modelled as a team orienteering problem

This work presents an improved model to solve the non-emergency patients transport (NEPT) service issues given the new rules recently established in Portugal. The model follows the same principle of the Team Orienteering Problem by selecting the patients to be included in the routes attending the maximum reduction in costs when compared with individual transportation. This model establishes the best sets of patients to be transported together. The model was implemented in AMPL and a compact formulation was solved using NEOS Server. A heuristic procedure based on iteratively solving Orienteering Problems is presented, and this heuristic provides good results in terms of accuracy and computation time. Euclidean instances as well as asymmetric real data gathered from Google maps were used, and the model has a promising performance mainly with asymmetric cost matrices.

A decision support system for a waste collection vehicle routing problem

The selective collection of municipal solid waste for recycling is a very complex and expensive process, where a major issue is to perform cost-efficient waste collection routes. Despite the abundance of commercially available software for fleet management, they often lack the capability to deal properly with sequencing problems and dynamic revision of plans and schedules during process execution. Our approach to achieve better solutions for the waste collection process is to model it as a vehicle routing problem, more specifically as a team orienteering problem where capacity constraints on the vehicles are considered, as well as time windows for the waste collection points and for the vehicles. The final model is called capacitated team orienteering problem with double time windows (CTOPdTW).We developed a genetic algorithm to solve routing problems in waste collection modelled as a CTOPdTW. The results achieved suggest possible reductions of logistic costs in selective waste collection.

Documentação pedagógica: estratégia de escuta e resposta a diferentes interesses em interação

Relatório de estágio de mestrado em Educação Pré-Escolar

Interacting and making personalized recommendations of places of interest to tourists

Advances in Intelligent Systems and Computing, 353