Desenvolvimento de uma prova de imunoadsorção enzimática para detecção de anticorpos contra Babesia bovis

Uma prova de imunoadsorção enzimática (ELISA) para detecção de anticorpos contra Babesia bovis foi desenvolvida e avaliada em comparação à imunofluorescência indireta (IFI). A sensibilidade e especificidade do ELISA, determinadas pela análise de 100 soros positivos de bovinos infectados experimentalmente com B. bovis e 108 soros negativos colhidos de bovinos livres de infecção por este hemoparasito, foram de 98,0% e 98,1%, respectivamente. Os valores preditivos positivo e negativo foram, respectivamente, 98,0% e 98,1% e a precisão do teste foi de 98,1%. Não foram detectadas reações cruzadas com 80 soros de bezerros experimentalmente inoculados com Babesia bigemina. O ELISA foi comparado à IFI usando 110 soros de rebanhos de área de estabilidade endêmica e 168 soros de rebanhos de áreas de instabilidade endêmica. em ambos os casos, houve concordância significativa (P=0,631 e 0,4725, respectivamente) entre os resultados demonstrados pelos dois testes. em um estudo epidemiológico realizado com o ELISA na região do Pantanal de Mato Grosso do Sul, com 1.365 soros de bovinos, 83,9% foram positivos para anticorpos contra B. bovis, caracterizando a região estudada como endemicamente estável.

Evaluation of an ELISA for detection of antibodies to Babesia bigemina in cattle and it's application in an epidemiological survey in Brazil

Um ensaio de imunoadsorção enzimática (ELISA) baseado em antígeno bruto foi avaliado na detecção de anticorpos contra Babesia bigemina. A sensibilidade e a especificidade do teste foram de 98,0% e 99,0%, respectivamente. Concordando com a alta especificidade do teste, não foram verificadas reações cruzadas com soros de bezerros inoculados três vezes com 10(7) merozoítos de Babesia bovis. Com relação à comparação do ELISA com a imunofluorescência indireta (IFAT) na detecção de anticorpos contra B. bigemina em bezerros experimentalmente infectados com cinco isolados brasileiros geograficamente distintos deste hemoparasito, o IFAT foi capaz de detectar anticorpos um dia antes do ELISA na maioria dos soros dos animais. Houve uma boa concordância entre os resultados encontrados no ELISA e no IFAT com soros de bovinos de região de estabilidade enzoótica (k=0.61). No entanto, não houve concordância entre os testes sorológicos com soros de animais de área de instabilidade enzoótica (k=0.33). O ELISA foi empregado em um inquérito epidemiológico com 1.367 soros de quatro municípios do Pantanal de Mato Grosso do Sul e caracterizou esta região como uma área de estabilidade enzoótica, uma vez que as prevalências variaram de 87,7 a 98,9%. Dessa forma, este ELISA, que apresentou alta sensibilidade, especificidade e desempenho similar ao IFAT, pode ser utilizado no diagnóstico sorológico de B. bigemina.

Levantamento fitossociológico das comunidades de plantas infestantes em áreas de produção de arroz irrigado cultivado sob diferentes sistemas de manejo

O presente trabalho de pesquisa teve como objetivo identificar a composição florística de comunidades de plantas daninhas presentes em áreas agrícolas de várzea, manejadas em diferentes sistemas. O trabalho foi desenvolvido em áreas de produção de arroz irrigado das cooperativas: Cooperativas Mista Rural Vale do Javaés e Cooperativa Agroindustrial Rio Formoso, em Formoso do Araguaia-TO. Foram separadas três áreas de 1 ha, sendo: 1 - área sem rotação de culturas (arroz/pousio) há mais de cinco anos; 2 - área com rotação de culturas (arroz/soja) há mais de cinco anos; 3 - área com rotação de culturas (arroz/melancia) há mais de dois anos. Para caracterização e estudo fitossociológico da comunidade infestante foi utilizado, como unidade amostral, um quadro (1,0 x 1,0 m), lançado aleatoriamente dentro da área de estudo (método do quadrado inventário), por meio de um caminhamento em ziguezague. Na área sem rotação, foram identificadas 8 famílias e 16 espécies, destacando-se a família Poaceae com maior número espécies; Fimbristylis miliacea (Cyperaceae) foi a espécie com o maior índice de importância relativa (84,46%). Na área com rotação arroz/soja, foram identificadas 8 famílias e 12 espécies, destacando-se as famílias Poaceae e Cyperaceae com maior número espécies; Cyperus esculentus (Cyperaceae) foi a espécie com o maior índice de importância relativa (91,4%). Na área com rotação arroz/melancia foram identificadas seis famílias e oito espécies, destacando-se as famílias Euphorbiaceae e Lamiaceae com maior número espécies; Physalis angulata (Solanaceae) foi a espécie com o maior índice de importância relativa (153,1%), seguida por Eclipta alba (Compositae) e Hyptis lophanta (Lamiaceae), com 40,45 e 37,6%, respectivamente.

Association of midazolam with ketamine in giant Amazon river turtles Podocnemis expansa breed in captivity

OBJETIVO: Avaliar os efeitos de duas associações anestésicas em tartarugas da Amazônia em (Podocnemis expansa). MÉTODOS: Vinte P. expansa, hígidas, de ambos os sexos, com massa corporal entre 1,0 e 1,5 kg, de um criatório comercial localizado no vale do rio Araguaia, Goiás, Brasil, foram distribuídas em dois grupos (G1 n=10 e G2 n=10). Cada grupo recebeu um protocolo sendo: P1 = midazolam (2 mg/kg IM) com cetamina (20 mg/kg IM) e P2 = midazolam (2 mg/kg IM) com cetamina (60 mg/kg IM), aplicados nos grupos G1 e G2, respectivamente. Os fármacos foram aplicados no membro torácico esquerdo. Os parâmetros clínicos avaliados foram: locomoção, relaxamento muscular, resposta aos estímulos dolorosos nos membros torácico direito e pelvinos e freqüência cardíaca. Essas avaliações foram feitas no tempo 0 (imediatamente após a injeção) e nos tempos 5, 10, 20, 30, 45, 60, 90, 120, 150 e 180 minutos após as injeções. RESULTADOS: O G2 apresentou maior freqüência cardíaca que o G1 e imobilização mais rápida e prolongada. CONCLUSÃO: As sedações obtidas por esses protocolos (P1 e P2) foram satisfatórias, sendo possível a contenção farmacológica para a coleta de amostras biológicas e exame físico em P. expansa.

Anticorpos contra Toxoplasma gondii em estudantes de medicina veterinária de Campo Grande, MS, Brasil

Amostras de soro obtidas de estudantes do curso de Medicina Veteriná ria da Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Campo Grande, MS, Brasil, foram examinadas para a presença de anticorpos contra Toxoplasma gondii. Dos 145 soros testados, 44 (30,34%) foram positivos na hemaglutinação, com título igual ou superior a 1:16. Não foram observadas associações entre as caracterí sticas epidemiológicas examinadas, tais como hábitos alimentares (ingestão de carne bovina crua ou malpassada, vegetais crus/não lavados, produtos lácteos não pasteurizados) ou contato constante com cães e a presença de anticorpos contra T. gondii, exceto pelo percentual significativamente maior de estudantes soropositivos que relataram ter contato freqüente com gatos (P=0,03).

Jatrowedione, a lathyrane diterpene from Jatropha weddelliana

3-Acetylaleuritolic acid, sitosterol and a novel lathyrane diterpene, jatrowedione, have been isolated from the roots of Jatropha weddelliana. The elucidation of the structure of the latter was accomplished by detailed NMR investigation, and the relative configuration was established by difference NOE experiments. (C) 1998 Published by Elsevier B.V. Ltd. All rights reserved.

Radiative properties of the skin and haircoat of cattle and other animals

Radiative properties (reflectance rho, transmittance tau, and absorptance alpha) were determined for wavelengths from 300 to 850 nm in the haircoat and the skin of water buffalo, deer (Pantanal deer, Blastocerus dichotomus), and cattle of the Holstein, Simmental, Canchim, Brangus, and Nelore breeds. The results showed that white hairs have higher rho (0.60 to 0.67) than the other coat colors, but the gray coats (mixed white and dark hair) of the Nelore cattle presented higher rho than that of the white coats of the European breeds at wavelengths lower than 600 nm. The light gray colored skin of the Canchim cattle had higher rho (0. 66) than the non-pigmented skin of Holstein (0.53). Red skins presented rho values higher than those of dark gray and black skins. Buffalo skin (dark gray) presented an average rho of 0.23+/-0.02 and alpha of 0.77+/-0.02. The red haircoat of the deer presented rho lower (0.37) than that of cattle of the same color (0.58). However, there was little difference between deer and cattle with respect to reflectance and absorptance of the skin. As for the spectral transmittance of the skin, it was very low and about the same for both species, until 600 nm. In the range 600 to 850 nm, the tau values for cattle rose to 0. 17, while those for deer increased only to 0.12.

A formação de trabalhadores brasileiros: a experiência do Colégio Isabel

Colegio Isabel was a project that aimed to "civilize" the indigenous children who lived in the Vale do Araguaia region, Goias Province, to educate national workers and, progressively, incorporate the lands owned by the Indians in the productive process. Colegio Isabel allowed its students a marginal integration in the society, assigning them functions that the so called "civilized" people considered degrading. The egress students lived an ambiguous and marginal life, regarding the original ethnic groups and the adopted society.

Persistence and stability of cichlid assemblages in neotropical floodplain lagoons

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Frugivory by the fish Brycon hilarii (Characidae) in western Brazil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Othonella araguaiana (Bivalvia, Megadesmidae) from the Corumbatai Formation (Midlle [Middle] Permian), Eastern Margin of the Parana Basin: systematic, evolutionary and biostratigraphic significances.

In this study, the occurrence of Othonella araguaiana Mendes, a rare bivalve species is reported for the first time in the Pinzonella illusa biozone, Middle Permian Corumbatai Formation, in the State of São Paulo. This species was originally described in coeval rocks of the Estrada Nova Formation (= Corumbatai) from the Alto Araguaia and Alto Garcas regions, State of Mato Grosso. The specimens of O. araguaiana were found in the base of a bioclastic sandstone bed, a proximal tempestite, in the middle of the Corumbatai Formation, in the city of Rio Claro, São Paulo State. The silicified shells and internal molds are well preserved, showing impressions of muscle scars and other internal anatomic characters (e.g., hinge), never illustrated by previous authors. In his original description, Mendes (1963) called attention to the similarity between O. araguaiana and Terraia aequilateralis, a common veneroid of the Corumbatai Formation. Conversely, Runnegar and Newell (1971) suggested that O. araguaiana belongs to Megadesmidae, being a junior synonym of Plesiocyprinella carinata (the commonest megadesmid of the Passa Dois Group). Our study indicates that O. araguaiana is indeed a megadesmid, but is distinct from the P. carinata. The new occurrence of O. araguaiana demonstrates that a) the paleobiogeographic distribution of this species is wider than previously thought (that it was restricted to the northern part of Parana Basin, Mato Grosso State); b) the molluscan fauna of the Corumbatai Formation (P. illusa biozone) in the State of São Paulo is more diverse and dominated by megadesmids; and c) the composition of the molluscan fauna of the Corumbatai Formation in Alto GarYas, State of Mato Grosso, is essentially the same as that of the P. illusa biozone of the eastern margin of the Parana Basin.

Modelo para o estudo da dinâmica evolutiva dos aspectos fisiográficos dos pantanais

This paper studies the applicability of a model to the evolutive dynamics of Pantanal physiography. The Miranda-Aquidauana Pantanal has developed as a pre-existent fluvial plain in the beginning, from Paraguay river, whose ancient marks, recognized by weathering alteration volumes, show components that make possible its relative age determination and consequently permit the comprehension of the ancient and recent dynamic evolutions, that are responsible for the present physiographic form.

Dinâmica da ocupação do solo bacia do Rio Itiquira, Mato Grosso, Brasil

The study area corresponds to the basin of the Itiquira river high course, totalling 5,361km2. In this area a study of the environmental dynamics was made, applying SIG techniques and satellite images of the years 1966, 1985 and 1996. In 1966 the areas of natural vegetation (forests and groves) occupied 90.64% of the total of the area, which was diminished to 60.57% in 1985 and to only 36.66% in 1996. In this process, 289,382ha of a total of 485,928ha of natural vegetal covering had been lost. At the same time, the agrarian surfaces (agriculture and pasture) that occupied only 0.52% of the total area in 1966, increased to 34.89% in 1985 and to 59.04% in 1996. In 30 years there was an increase of 313,725ha of cultivated lands, corresponding to about 113 times the land occupation of the year of 1966 (2,798ha). The areas classified as urban show a gradual increase since 1966, from 39ha in 1996 to 58ha in 1985, and to 178ha in 1996. The other classes of soil use and occupation (reforestement areas, uncovered and affected by fire and humid areas) added were smaller than 4,27% in 1996.

U-Pb geochronology of the southern Brasília belt (SE-Brazil): Sedimentary provenance, Neoproterozoic orogeny and assembly of West Gondwana

The Brasília belt borders the western margin of the São Francisco Craton and records the history of ocean opening and closing related to the formation of West Gondwana. This study reports new U-Pb data from the southern sector of the belt in order to provide temporal limits for the deposition and ages of provenance of sediments accumulated in passive margin successions around the south and southwestern margins of the São Francisco Craton, and date the orogenic events leading to the amalgamation of West Gondwana. Ages of detrital zircons (by ID-TIMS and LA-MC-ICPMS) were obtained from metasedimentary units of the passive margin of the São Francisco Craton from the main tectonic domains of the belt: the internal allochthons (Araxá Group in the Áraxá and Passos Nappes), the external allochthons (Canastra Group, Serra da Boa Esperança Metasedimentary Sequence and Andrelândia Group) and the autochthonous or Cratonic Domain (Andrelândia Group). The patterns of provenance ages for these units are uniform and are characterised as follows: Archean- Paleoproterozoic ages (3.4-3.3, 3.1-2.7, and 2.5-2.4Ga); Paleoproterozoic ages attributed to the Transamazonian event (2.3-1.9Ga, with a peak at ca. 2.15Ga) and to the ca. 1.75Ga Espinhaço rifting of the São Francisco Craton; ages between 1.6 and 1.2Ga, with a peak at 1.3Ga, revealing an unexpected variety of Mesoproterozoic sources, still undetected in the São Francisco Craton; and ages between 0.9 and 1.0Ga related to the rifting event that led to the individualisation of the São Francisco paleo-continent and formation of its passive margins. An amphibolite intercalation in the Araxá Group yields a rutile age of ca. 0.9Ga and documents the occurrence of mafic magmatism coeval with sedimentation in the marginal basin. Detrital zircons from the autochthonous and parautochthonous Andrelândia Group, deposited on the southern margin of the São Francisco Craton, yielded a provenance pattern similar to that of the allochthonous units. This result implies that 1.6-1.2Ga source rocks must be present in the São Francisco Craton. They could be located either in the cratonic area, which is mostly covered by the Neoproterozoic epicontinental deposits of the Bambuí Group, or in the outer paleo-continental margin, buried under the allochthonous units of the Brasília belt. Crustal melting and generation of syntectonic crustal granites and migmatisation at ca. 630Ma mark the orogenic event that started with westward subduction of the São Francisco plate and ended with continental collision against the Paraná block (and Goiás terrane). Continuing collision led to the exhumation and cooling of the Araxá and Passos metamorphic nappes, as indicated by monazite ages of ca. 605Ma and mark the final stages of tectonometamorphic activity in the southern Brasília belt. Whilst continent-continent collision was proceeding on the western margin of the São Francisco Craton along the southern Brasília belt, eastward subduction in the East was generating the 634-599Ma Rio Negro magmatic arc which collided with the eastern São Francisco margin at 595-560Ma, much later than in the Brasília belt. Thus, the tectonic effects of the Ribeira belt reached the southernmost sector of the Brasília belt creating a zone of superposition. The thermal front of this event affected the proximal Andrelândia Group at ca. 588Ma, as indicated by monazite age. The participation of the Amazonian craton in the assembly of western Gondwana occurred at 545-500Ma in the Paraguay belt and ca. 500Ma in the Araguaia belt. This, together with the results presented in this work lead to the conclusion that the collision between the Paraná block and Goiás terrane with the São Francisco Craton along the Brasília belt preceded the accretion of the Amazonian craton by 50-100 million years. © 2003 Elsevier B.V. All rights reserved.

Tolerância de perdas de solo na bacia do rio Itiquira, Mato Grosso, Brasil

The geographic area of the present study corresponds to the basin of the Itiquira river high course (Figure 1), in the portion that extends from the tributaries of its source, in the east of the plateau Correntes/Itiquira, in the neighbourhoods the city of Alto Garç as, to the scarp of the São Jerônimo mountain range, toward the west of Itiquira, in the state of Mato Grosso, totalizing 5,361 km 2. The area is placed in the eastern part of the Alto Paraguai basin, in the western portion of the Paraná Sedimentary Basin plateau. Through GIS techniques, it was possible to asses total soil losses from the Itiquira river basin, considering the years of 1966, 1985 and 1996, being based on the Universal Soil Loss Equation. Thus, in 1966 the basin lost 201,546.94 ton of soil, with an average loss of 0.37 ton/ha/year. Considering that the total area of the Itiquira river basin is of 536,100 ha, while in 1985 the soil losses had passed to 1,760,833.40 ton, with an increase of approximately 8.5 times. The average of soil losses in 1985 was of 3.28 ton/ha/year. In 1996 the basin lost 1,662,043.24 ton, with a reduction of only 9.4% in relation to 1985 but, in relation the 1966, the increase continued in the order of 8 times. The average losses per hectare in this year was in the order of 3.10 ton/ha/year (Chart 1). The map of potential of the laminar erosion for 1966 (Figure 3), shows to the highest values in small areas, situated in the northeast of the area, in Alto Garças, with values between 10 the 20 ton/ha/year and some spots in sources of the Itiquira and Ariranha rivers, with values between 1 the 5 ton/ha/year. In a general way, however, the area presents low soil loss for laminar erosion in this year, with inferior values to 1 ton/ha/year. The higher class of erosion, over 10 ton/ha/year, occupied 2,947 ha in 1966. In the year of 1985 (Figure 4), the erosive process spread over the entire studied area, and the class of erosion over of 10 ton/ha/year, already started to occupy 78,437 ha, implying an increase of approximately 27 times in 19 years. A strong increment in the erosive process was noticed in the western part of the area, to along the BR-163 road, exactly where great areas of natural vegetation (open pasture) had been transformed in culture and pasture areas. In the north-eastern part of the area it was also noticed an increment in the erosive process in agreement with the increase of culture areas and reduction of the natural areas, but it was not of so intense form as in the western portion of the area. In the year of 1996 (Figure 5), the class of erosion over of 10 ton/ha/year had diminished for a total of 53.499 ha noticing a retraction of the erosive process in the western part of the area, alongside the BR-163 road. On the other hand, it occurred a strong increment in the northern part of the area, in the neighbourhoods of the city of Alto Garças, alongside the BR-364 road and part of the MT-040 road. In a general way, in the outskirts of the city of Itiquira, in the central part of the area, it was verified an increase of the amount of zones with erosion between 0-1 ton/ha/year, passing to the immediately superior class, of 1-3 ton/ha/year; scarce data of hydrosedimentology in the UHE Itiquira (1999), shows good agreement with the values gotten for the EUPS (Chart 2). Based on the hydric classification proposal for FAO (1967) (Chart 3), it is noticed that areas with high degree of erosion (> 50) in the analysed area are very restricted, occupying 493 ha in 1985 and 332 ha in 1996 (Chart 4). In 1996 appeared as isolated spots in the north of Itiquira and Alto Garças, however beyond limits of the Itiquira river basin. These areas require special cares in its use as agricultural areas. Aiming at identifying and indicating the areas of potential risk of erosion and that need implementation of conservation practices, it was elaborated the map of limit of tolerance to the soil losses. In 1966 (Figure 6), areas with soil losses over of the tolerable were restricted to small spots located in the eastern part of the area, occupying 0.43% of the total of the area; already in 1985 (Figure 7), this percentage passed to 5.86%, spreading for all the area; in 1996 (Figure 8) it is observed a fast reduction of the areas with soil losses over of the tolerance limit, passing 5.43% of the total of the area. All the areas with losses over of the tolerable value must be considered as risk areas and were done in these areas studies for implementation of conservation practices.