Extraction of polymeric galactoglucomannans from spruce wood by pressurized hot water

The major type of non-cellulosic polysaccharides (hemicelluloses) in softwoods, the partly acetylated galactoglucomannans (GGMs), which comprise about 15% of spruce wood, have attracted growing interest because of their potential to become high-value products with applications in many areas. The main objective of this work was to explore the possibilities to extract galactoglucomannans in native, polymeric form in high yield from spruce wood with pressurised hot-water, and to obtain a deeper understanding of the process chemistry involved. Spruce (Picea abies) chips and ground wood particles were extracted using an accelerated solvent extractor (ASE) in the temperature range 160 – 180°C. Detailed chemical analyses were done on both the water extracts and the wood residues. As much as 80 – 90% of the GGMs in spruce wood, i.e. about 13% based on the original wood, could be extracted from ground spruce wood with pure water at 170 – 180°C with an extraction time of 60 min. GGMs comprised about 75% of the extracted carbohydrates and about 60% of the total dissolved solids. Other substances in the water extracts were xylans, arabinogalactans, pectins, lignin and acetic acid. The yields from chips were only about 60% of that from ground wood. Both the GGMs and other non-cellulosic polysaccharides were extensively hydrolysed at severe extraction conditions when pH dropped to the level of 3.5. Addition of sodium bicarbonate increased the yields of polymeric GGMs at low additions, 2.5 – 5 mM, where the end pH remained around 3.9. However, at higher addition levels the yields decreased, mainly because the acetyl groups in GGMs were split off, leading to a low solubility of GGMs. Extraction with buffered water in the pH range 3.8 – 4.4 gave similar yields as with plain water, but gave a higher yield of polymeric GGMs. Moreover, at these pH levels the hydrolysis of acetyl groups in GGMs was significantly inhibited. It was concluded that hot-water extraction of polymeric GGMs in good yields (up to 8% of wood) demands appropriate control of pH, in a narrow range about 4. These results were supported by a study of hydrolysis of GGM at constant pH in the range of 3.8 – 4.2 where a kinetic model for degradation of GGM was developed. The influence of wood particle size on hot-water extraction was studied with particles in the range of 0.1 – 2 mm. The smallest particles (< 0.1 mm) gave 20 – 40% higher total yield than the coarsest particles (1.25 – 2 mm). The difference was greatest at short extraction times. The results indicated that extraction of GGMs and other polysaccharides is limited mainly by the mass transfer in the fibre wall, and for coarse wood particles also in the wood matrix. Spruce sapwood, heartwood and thermomechnical pulp were also compared, but only small differences in yields and composition of extracts were found. Two methods for isolation and purification of polymeric GGMs, i.e. membrane filtration and precipitation in ethanol-water, were compared. Filtration through a series of membranes with different pore sizes separated GGMs of different molar masses, from polymers to oligomers. Polysaccharides with molar mass higher than 4 kDa were precipitated in ethanol-water. GGMs comprised about 80% of the precipitated polysaccharides. Other polysaccharides were mainly arabinoglucuronoxylans and pectins. The ethanol-precipitated GGMs were by 13C NMR spectroscopy verified to be very similar to GGMs extracted from spruce wood in low yield at a much lower temperature, 90°C. The obtained large body of experimental data could be utilised for further kinetic and economic calculations to optimise technical hot-water extractionof softwoods.

Ohjelmistoradion hyödyntäminen tiedonsiirron tutkimisessa LVDC-verkossa

Työssä tutkitaan tiedonsiirtoa eri modulaatioilla, bittinopeuksilla ja amplitudin voimakkuuksilla ja tuloksia tarkastellaan Bit Error Ration avulla. Signaaleja siirrettiiin myös koodattuna ja vertailtiin koodauksen etuja ja haittoja verrattuna koodaamattomaan tietoon. Datavirta kulkee AXMK-kaapelissa, joko tasasähkön mukana, tai maadoituskaapelissa. Tuloksissa havaittiin, että suurempi bittinopeus ei kasvattanut häviöiden määrää. Koodauksen käyttö toisaalta vähenti bittivirheiden määrää.

Modeling, optimizing and simulating robot calibration with accuracy improvement

This work describes techniques for modeling, optimizing and simulating calibration processes of robots using off-line programming. The identification of geometric parameters of the nominal kinematic model is optimized using techniques of numerical optimization of the mathematical model. The simulation of the actual robot and the measurement system is achieved by introducing random errors representing their physical behavior and its statistical repeatability. An evaluation of the corrected nominal kinematic model brings about a clear perception of the influence of distinct variables involved in the process for a suitable planning, and indicates a considerable accuracy improvement when the optimized model is compared to the non-optimized one.

Model for subchannel friction factors and flow redistribution in wire-wrapped rod bundles

The fuel element of LMFBR consists of a bundle of rods wrapped with an helical wire as spacer, surrounded by an hexagonal duct. In the present work, a semi-empirical model is developed to calculate bundle average and subchannel based friction factors and flow redistribution. The obtained results were compared to experimental data and they were considered satisfactory for wide range of geometrical parameters.

Parameter optimization of AC rectangular wave outputs for aluminum cold wire GTAW

The objective of this work was to optimize the parameter setup for GTAW of aluminum using an AC rectangular wave output and continuous feeding. A series of welds was carried-out in an industrial joint, with variation of the negative and positive current amplitude, the negative and positive duration time, the travel speed and the feeding speed. Another series was carried out to investigate the isolate effect of the negative duration time and travel speed. Bead geometry aspects were assessed, such as reinforcement, penetration, incomplete fusion and joint wall bridging. The results showed that currents at both polarities are remarkably more significant than the respective duration times. It was also shown that there is a straight relationship between welding speed and feeding speed and this relationship must be followed for obtaining sound beads. A very short positive duration time is enough for arc stability achievement and when the negative duration time is longer than 5 ms its effect on geometry appears. The possibility of optimizing the parameter selection, despite the high inter-correlation amongst them, was demonstrate through a computer program. An approach to reduce the number of variables in this process is also presented.

Static pressure and wall shear stress distributions in air flow in a seven wire-wrapped rod bundle

An experimental investigation is performed in a turbulent flow in a seven wire-wrapped rod bundle, mounted in an open air facility. Static pressure distributions are measured on central and peripheral rods. By using a Preston tube, the wall shear stress profiles are experimentally obtained along the perimeter of the rods. The geometric parameters of the test section are P/D=1.20 and H/D=15. The measuring section is located at L/D=40 from the air inlet. It is observed that the dimensionless static pressure and wall shear stress profiles are nearly independent of the Reynolds number and strongly dependent of the wire-spacer position, with abrupt variations of the parameters in the neighborhood of the wires.

Experimental validation of a 3-D vision-based measurement system applied to robot calibration

One of the problems that slows the development of off-line programming is the low static and dynamic positioning accuracy of robots. Robot calibration improves the positioning accuracy and can also be used as a diagnostic tool in robot production and maintenance. A large number of robot measurement systems are now available commercially. Yet, there is a dearth of systems that are portable, accurate and low cost. In this work a measurement system that can fill this gap in local calibration is presented. The measurement system consists of a single CCD camera mounted on the robot tool flange with a wide angle lens, and uses space resection models to measure the end-effector pose relative to a world coordinate system, considering radial distortions. Scale factors and image center are obtained with innovative techniques, making use of a multiview approach. The target plate consists of a grid of white dots impressed on a black photographic paper, and mounted on the sides of a 90-degree angle plate. Results show that the achieved average accuracy varies from 0.2mm to 0.4mm, at distances from the target from 600mm to 1000mm respectively, with different camera orientations.

Hot tool temperature analysis though infrared images

Textile manufacture occupies a prominent place in the national economy. Because of its importance researches have been made on the development of new materials, equipment and methods used in the production process. The cutting of textiles starts in the basic stage, to be followed by the process of the making of clothes and other articles. In the hot cutting of fabric, one of the variables of great importance in the control of the process is the contact temperature between the tool and the fabric. This work presents a technique for the measurement of the temperature based on the processing of infrared images. With this purpose, it was developed a system which is composed of an infrared camera, a framegrabber PC board and a software which analyses the punctual temperature in the cut area enabling the operator to achieve the necessary control of other variables involved in the process.

On-site calibration of a phase fraction meter by an inverse technique

The formal calibration procedure of a phase fraction meter is based on registering the outputs resulting from imposed phase fractions at known flow regimes. This can be straightforwardly done in laboratory conditions, but is rarely the case in industrial conditions, and particularly for on-site applications. Thus, there is a clear need for less restrictive calibration methods regarding to the prior knowledge of the complete set of inlet conditions. A new procedure is proposed in this work for the on-site construction of the calibration curve from total flown mass values of the homogeneous dispersed phase. The solution is obtained by minimizing a convenient error functional, assembled with data from redundant tests to handle the intrinsic ill-conditioned nature of the problem. Numerical simulations performed for increasing error levels demonstrate that acceptable calibration curves can be reconstructed, even from total mass measured within a precision of up to 2%. Consequently, the method can readily be applied, especially in on-site calibration problems in which classical procedures fail due to the impossibility of having a strict control of all the input/output parameters.

Dual light barrier method for six degrees of freedom tool center point calibration of an industrial robot

Tool center point calibration is a known problem in industrial robotics. The major focus of academic research is to enhance the accuracy and repeatability of next generation robots. However, operators of currently available robots are working within the limits of the robot´s repeatability and require calibration methods suitable for these basic applications. This study was conducted in association with Stresstech Oy, which provides solutions for manufacturing quality control. Their sensor, based on the Barkhausen noise effect, requires accurate positioning. The accuracy requirement admits a tool center point calibration problem if measurements are executed with an industrial robot. Multiple possibilities are available in the market for automatic tool center point calibration. Manufacturers provide customized calibrators to most robot types and tools. With the handmade sensors and multiple robot types that Stresstech uses, this would require great deal of labor. This thesis introduces a calibration method that is suitable for all robots which have two digital input ports free. It functions with the traditional method of using a light barrier to detect the tool in the robot coordinate system. However, this method utilizes two parallel light barriers to simultaneously measure and detect the center axis of the tool. Rotations about two axes are defined with the center axis. The last rotation about the Z-axis is calculated for tools that have different width of X- and Y-axes. The results indicate that this method is suitable for calibrating the geometric tool center point of a Barkhausen noise sensor. In the repeatability tests, a standard deviation inside robot repeatability was acquired. The Barkhausen noise signal was also evaluated after recalibration and the results indicate correct calibration. However, future studies should be conducted using a more accurate manipulator, since the method employs the robot itself as a measuring device.

Vi åt fisken och jag gillar det. Tempus i finska gymnasisters skrivna berättelser

Tässä tutkielmassa tarkoituksena on tutkia aikamuotojen käyttöä suomenkielisten lukiolaisten ruotsiksi kirjoittamissa kertomuksissa. Lukiolaisten tekstit on arvioitu yhteisen eurooppalaisen viitekehyksen kielitaidon tasoasteikon mukaan, joten tutkimuksen päätavoitteena on vertailla eroja eri taitotasojen välillä. Tutkimuksessa mukana olevat taito-tasot ovat A1, A2 ja B1. Lisäksi tarkoituksena on vertailla suomalaisten lukiolaisten aikamuotojen käyttöä verrokkiryhmänä toimivien samanikäisten ruotsalaisten lukiolaisten aikamuotojen käyttöön. Edelleen analyysiosassa selvitetään, noudattavatko suomen- ja ruotsinkieliset lukiolaiset teksteissään tempusharmoniaa. Tutkimuksen materiaali on osa projektia Toisen kielen oppimisen polut (Topling). Tämän tutkielman materiaalina käytetään yhteensä 130 suomenkielisen lukiolaisen kirjoittamaa tarinaa sekä verrokkimateriaalina 31 ruotsalaisen lukiolaisen kirjoittamaa tarinaa. Suomenkielisten lukiolaisten kirjoitelmat on kerätty pitkittäistutkimuksena kahdessa osassa, ensimmäiset lukion kurssilla 1 tai 2 ja toiset kurssilla 3. Topling-projektin puitteissa kerätyt kirjoitelmat on arvioitu Eurooppalaisen viitekehyksen taitotasoasteikkojen mukaan. Tutkimusmetodit ovat pääasiassa kvantitatiivisia. Tulokset esitellään taulukoi-en muodossa frekvenssein ja prosentein. Lisäksi käytetään x²-testiä eri taitotasojen välisten erojen selvittämiseksi. Tuloksia tarkastellaan ryhmätasolla. Kvalitatiivisia metodeja käytetään osittain tarkasteltaessa ruotsalaisten lukiolaisten aikamuotojen käyttöä sekä tempusharmoniaa analysoitaessa. Tuloksista käy ilmi, että imperfekti oli käytetyin ja preesens toiseksi käytetyin aikamuoto koko materiaalissa. Imperfektiä käytettiin enemmän taitotasolla B1 kuin alemmilla taitotasoilla, kun taas preesens oli yleisempi alemmilla tasoilla. Muiden aikamuotojen (perfekti, pluskvamperfekti, futuuri ja mennen ajan futuuri) osuus materiaalissa oli melko pieni. Tutkimus osoitti, että lukiolaiset käyttivät eri aikamuotoja melko hyvin. Koko materiaalissa oikeaa aikamuotoa käytettiin 82,5 %:ssa tapauksista. Tasolla B1 oikeellisuusprosentti oli kaikkein korkein. Materiaalissa imperfektiä käytettiin liian vähän, kun taas preesensiä käytettiin liikaa. Toisin sanoen lukiolaiset yksinkertaistivat tekstejään käyttämällä helpompia preesens-muotoja vaikeampien imperfektimuotojen sijasta. Tutkimuksen perusteella ei voida tehdä yleistyksiä monimutkaisempien aikamuotojen (perfekti, pluskvamperfekti, futuuri ja menneen ajan futuuri) käytöstä, sillä niiden osuus materiaalissa oli vähäinen. Mitä tulee infiniittisiin verbimuotoihin, infinitiiveihin ja supiineihin, käytettiin niitä useammin aikamuotoon taivutettujen verbien sijasta kuin päinvastoin. Ruotsalaiset lukiolaiset käyttivät kertomuksissaan eri aikamuotoja monipuolisemmin kuin suomenkieliset ikätoverinsa. Lisäksi tutkimuksessa kävi ilmi, että sekä suomalaisilla että ruotsalaisilla lukiolaisilla oli vaikeuksia noudattaa tempusharmoniaa.

A 37-kb restriction map of the human immunoglobulin lambda variable locus, VB cluster, harboring four functional genes and two non-coding Vl sequences

The human immunoglobulin lambda variable locus (IGLV) is mapped at chromosome 22 band q11.1-q11.2. The 30 functional germline v-lambda genes sequenced untill now have been subgrouped into 10 families (Vl1 to Vl10). The number of Vl genes has been estimated at approximately 70. This locus is formed by three gene clusters (VA, VB and VC) that encompass the variable coding genes (V) responsible for the synthesis of lambda-type Ig light chains, and the Jl-Cl cluster with the joining segments and the constant genes. Recently the entire variable lambda gene locus was mapped by contig methodology and its one- megabase DNA totally sequenced. All the known functional V-lambda genes and pseudogenes were located. We screened a human genomic DNA cosmid library and isolated a clone with an insert of 37 kb (cosmid 8.3) encompassing four functional genes (IGLV7S1, IGLV1S1, IGLV1S2 and IGLV5a), a pseudogene (VlA) and a vestigial sequence (vg1) to study in detail the positions of the restriction sites surrounding the Vl genes. We generated a high resolution restriction map, locating 31 restriction sites in 37 kb of the VB cluster, a region rich in functional Vl genes. This mapping information opens the perspective for further RFLP studies and sequencing

Imp. Caes. Carolo VI Augusto pio victori gentis suae sideri felicissimo chorographiam hanc exhibentem primar. regionum Habspurgicar. situm positumq. ... pietatis supplex dicat provincia Brisgoia.

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