931 resultados para height of instrument


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The effectiveness of ventilation flows is considered from the perspective of buoyancy (or heat) removal from a space. This perspective is distinct from the standard in which the effectiveness is based on the concentrations of a neutrally buoyant contaminant/passive tracer. Three new measures of effectiveness are proposed based on the ability of a flow to flush buoyancy from a ventilated space. These measures provide estimates of instantaneous and time-averaged effectiveness for the entire space, and local effectiveness at any height of interest. From a generalisation of the latter, a vertical profile of effectiveness is defined. These measures enable quantitative comparisons to be made between different flows and they are applicable when there is a difference in density (as is typical due to temperature differences) between the interior environment and the replacement air. Applications, therefore, include natural ventilation, hybrid ventilation and a range of forced ventilation flows. Finally, we demonstrate how the ventilation effectiveness of a room may be assessed from simple traces of temperature versus time. © 2006 Elsevier Ltd. All rights reserved.

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The airflow and thermal stratification produced by a localised heat source located at floor level in a closed room is of considerable practical interest and is commonly referred to as a 'filling box'. In rooms with low aspect ratios H/R ≲ 1 (room height H to characteristic horizontal dimension R) the thermal plume spreads laterally on reaching the ceiling and a descending horizontal 'front' forms separating a stably stratified, warm upper region from cooler air below. The stratification is well predicted for H/R ≲ 1 by the original filling box model of Baines and Turner (J. Fluid. Mech. 37 (1968) 51). This model represents a somewhat idealised situation of a plume rising from a point source of buoyancy alone-in particular the momentum flux at the source is zero. In practical situations, real sources of heating and cooling in a ventilation system often include initial fluxes of both buoyancy and momentum, e.g. where a heating system vents warm air into a space. This paper describes laboratory experiments to determine the dependence of the 'front' formation and stratification on the source momentum and buoyancy fluxes of a single source, and on the location and relative strengths of two sources from which momentum and buoyancy fluxes were supplied separately. For a single source with a non-zero input of momentum, the rate of descent of the front is more rapid than for the case of zero source momentum flux and increases with increasing momentum input. Increasing the source momentum flux effectively increases the height of the enclosure, and leads to enhanced overturning motions and finally to complete mixing for highly momentum-driven flows. Stratified flows may be maintained by reducing the aspect ratio of the enclosure. At these low aspect ratios different long-time behaviour is observed depending on the nature of the heat input. A constant heat flux always produces a stratified interior at large times. On the other hand, a constant temperature supply ultimately produces a well-mixed space at the supply temperature. For separate sources of momentum and buoyancy, the developing stratification is shown to be strongly dependent on the separation of the sources and their relative strengths. Even at small separation distances the stratification initially exhibits horizontal inhomogeneity with localised regions of warm fluid (from the buoyancy source) and cool fluid. This inhomogeneity is less pronounced as the strength of one source is increased relative to the other. Regardless of the strengths of the sources, a constant buoyancy flux source dominates after sufficiently large times, although the strength of the momentum source determines whether the enclosure is initially well mixed (strong momentum source) or stably stratified (weak momentum source). © 2001 Elsevier Science Ltd. All rights reserved.

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Size self-scaling effect in stacked InAs/In0.52Al0.48As nanowires on InP substrates is revealed, i.e., the base width and height of the InAs nanowires have clear proportional dependence on thickness of the InAlAs spacer layer used in different samples. The photoluminescence wavelength from different samples, which varies between 1.3 and 1.9 mum, is also found closely correlated to the size self-scaling effect. This phenomenon can be well explained in the context of formation mechanism and growth features of the InAs/InAlAs nanowire arrays. The finding illustrates a degree of freedom to control the structural and optical properties of strained self-organized nanostructures. (C) 2004 American Institute of Physics.

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Self-organized InAs quantum wires (QWRs) were fabricated on the step edges of the GaAs (331)A surface by molecular beam epitaxy. The lateral size of InAs QWRs was saturated by the terrace width (i.e., 90 nm) while the size along the step lines increased with the increasing thicknesses of the InAs layers, up to 1100 nm. The height of InAs QWRs varied from 7.9 nm to 13 nm. The evolution of the morphology of InAs QWRs was attributed to the diffusion anisotropy of In adatoms.

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The theoretical method to design negative refractive index metamaterials by single negative permittivity metamaterials is presented. By designing the electric and magnetic response metamaterials separately, the complexity of the design work can be simplified a lot. For the magnetic response metamaterials, the metallic post structure is adopted. Varying the height of the post, the response wavelength can be adjusted linearly. For electric metamaterials, wire-mesh structure is adopted. The effective material parameters, including refractive index, impedance, permittivity and permeability are given. Such a structure has negative refractive index during a broad frequency band and easy to design.

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The structure of micro-LEDs was optimized designed. Optical, electrical and thermal characteristics of micro-LEDs were improved. The optimized design make micro-LEDs suitable for high-power device. The light extraction efficiency of micro-LEDs was analyzed by the means of ray tracing. The results shows that increasing the inclination angle of sidewall and height of mesa, and reducing the absorption of p and n electrode can enhance the light extraction efficiency of micro-LEDs. Furthermore, the total light output power can be boosted by increasing the density of micro-structures on the device. The high-power flip-chip micro-LEDs were fabricated, which has higher quantum efficiency than conventional BALED's. When the number of microstructure in micro-LEDs was increased by 57%, the light output power was enhanced 24%. Light output power is 82.88mW at the current of 350mA and saturation current is up to 800mA, all of these are better than BALED which was fabricated in the same epitaxial wafer. The IN characteristics of micro-LEDs are almost identical to BALED.

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Pt/AlGaN/AlN/GaN Schottky diodes have been fabricated and characterized for H-2 sensing. Platinum (Pt) with a thickness of 20nm was evaporated on the sample to form the Schottky contact. The ohmic contact, formed by evaporated Ti/Al/Ni/Au metals, was subsequently annealed by a rapid thermal treatment at 860 degrees C for 30 s in N-2 ambience. Both the forward and reverse current of the device increased greatly when exposed to H-2 gas. The sensor's responses under different hydrogen concentrations from 500ppm to 10% H-2 in N-2 at 300K were investigated. A shift of 0.45V at 297K is obtained at a fixed forward current for switching from N-2 to 10% H-2 in N-2. Time response of the sensor at a fixed bias of 0.5 V was also measured. The turn-on response of the device was rapid, while the recovery of the sensor at N-2 atmosphere was rather slow. But it recovered quickly when the device was exposed to the air. The decrease in the barrier height of the diode was calculated to be about 160meV upon introduction of 10% H-2 into the ambient. The sensitivity of the sensor is also calculated. Some thermodynamics analyses have been done according to the Langmuir isotherm equation.

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It is shown that transmission and reflection group delay times in an asymmetric single quantum barrier are greatly enhanced by the transmission resonance when the energy of incident particles is larger than the height of the barrier. The resonant transmission group delay is of the order of the quasibound state lifetime in the barrier region. The reflection group delay can be either positive or negative, depending on the relative height of the potential energies on the two sides of the barrier. Its magnitude is much larger than the quasibound state lifetime. These predictions have been observed in microwave experiments. (c) 2005 Elsevier B.V. All rights reserved.

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A two-dimensional atomic scattering theory is developed for scattering of electrons by a circularly symmetric quantum structure in the two-dimensional electron gas. It is found that the scattering cross section oscillates as a function of ka where k is the electron wave vector and a is the radius of the cylindrical potential barrier. If there is a quantum well inside the potential barrier, there appears a series of sharp resonant-tunneling peaks superposed on the original scattering-cross-section curves. The width of the resonant-tunneling peak depends sensitively on the thickness, the height of the potential barrier, and the electron energy.

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A diffractive microlens with a cascade focal plane along the main optical axis of the device is fabricated using a low-cost technique mainly including single mask ultraviolet (UV) photolithography and dual-step KOH:H2O etching. Based on the evolutionary behavior of converse pyramid-shaped microholes (CPSMs) preshaped over a {100}-oriented silicon wafer in KOH etchant, the first-step KOH etching is performed to transfer initial square micro-openings in a SiO2 film grown by plasma enhanced chemical vapor deposition (PECVD) and patterned by single mask UV-photolithography, into CPSMs with needed dimension. After completely removing a thinned SiO2 mask, basic annular phase steps with a relatively steep sidewall and scheduled height can be shaped in the overlapped etching region between the neighboring silicon concave-arc microstructures evolved from CPSMs through the second-step KOH etching. Morphological measurements demonstrate a desirable surface of the silicon microlens with a roughness in nanometer scale and the feature height of the phase steps formed in the submicrometer range. Conventional optics measurements of the plastic diffractive microlens obtained by further hot embossing the fine microrelief phase map over the nickel mask made through a common electrochemical method indicate a highly efficient cascaded focusing performance.

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混农季节性放牧(agropastoral transhumance)通过作物种植和畜牧生产相结合的方式对不同海拔高度带上的资源进行相互补充利用,在亚洲兴都库什地区、青藏高原、横断山、东部及南部非洲、南美安第斯地区等具有悠久的历史。这种传统的生计系统几千年以来一直是居住在该地区的人类社会和自然生态系统相互作用的主要形式之一。这种传统的资源利用方式与山地自然植被以及特殊的山地人类文化和社会特征具有密切的协同演变关系。认识和理解这一关系,是山地生态学和人类学的核心科学问题之一。近年来,山地生态系统的多重功能性及动态演变对山区社会经济可持续发展的重要意义受到人们的不断关注。本文通过对云南省德钦县的12个自然村的混农季节性放牧以及对云南德钦、四川壤塘等山地植被格局特别是高海拔地带植被格局的的详细调查,探讨青藏高原东缘地区混农季节性放牧的主要特征、系统构成及相互关系,及其在全球变化、经济全球化和市场化及现代化过程中的变化趋势,分析混农季节性放牧与高山林线格局及生态系统的互动关系,旨在探讨山地地区人类活动与自然生态系统之间的互动关系,从而为山区社会经济可持续发展、环境建设和生物多样性保护等国家战略提供理论依据。 调查结果表明,混农季节性放牧是一种适应青藏高原东部高山峡谷地区环境因子及自然资源呈明显的垂直分布、资源数量稀少而时空分布异质性极高的生存环境的一种传统经济形式。这种传统的畜牧业的主要生产目的仍然是提供当地基本生存所需的产品,饲养牲口的种类和数量取决于农户的当地需求并且受资源的限制,因而维持在比较低的水平的。分布在不同海拔高度的放牧资源在一年中被牲口利用的时间也不同,互为补充,共同构成混农季节性放牧的资源基础。根据各社区永久居住点的位置和该村的土地资源特别是牧草地资源的分布范围,牲口迁移的距离和格局有较大的差异。。天然牧场仍然是最主要的畜牧业生产资源。混农季节性放牧中的农业系统和牧业系统互为补充,共同构成调查地区完整的的生计系统,农耕活动为放牧活动提供精饲料如粮食等和冬季饲料如秸秆, 其数量往往成为家庭畜牧业生产规模的主要决定因子之一。 通过对牲口数量和结构、牲口的时空迁移格局、牧业活动在整个经济活动中的相对重要性以及牧业活动和作物种植的关系方面的研究分析,混农季节性放牧在近几十年发生了深刻的变化。主要表现在牲口数量总体下降,牲口组成发生变化,牲口移动性降低、牧业活动的经济重要性下降以及牧业活动和种植活动之间的相互依存度降低等。上述变化的根本驱动力是发生在当地、地区及全球尺度上的环境、政治、社会经济、技术和文化等的变化,从而造成当地群众畜牧生产目标、土地利用和劳动力的分布等发生了变化。当地生计系统发生的改变可能会带来对方面而深刻的政治、社会经济、文化和生态影响。 混农季节性放牧这种古老的传统生计策略面临着许多挑战,如冬季饲料短缺、草场退化、缺乏市场竞争力、经济重要性降低、对年轻人缺乏吸引力、国家缺乏专门的政策指导等。与此同时,经济全球化、市场经济、新技术的应用、替代生计机会的增加、国家对于山地生态系统的作用的重新定位等也为传统生计系统转型、实现社会与生态共赢创造了机遇。 混农季节性放牧活动对亚高山及树线交错带生态系统系统的互动方式主要体现在以下几个方面:(1)牲口啃食、践踏等影响森林群落更新,改变森林群落的组成和结构,从而影响森林群落的演替进程和植被格局。林线边缘是搭建夏棚的首选地点,因此林线及树线交错地带就成了牲口活动的主要场所之一;(2)利用火烧开辟、维持和改良高山牧场; 3)在亚高山火灾迹地的放牧活动能够阻止火烧迹地的顺向演替; 4)牧民在林线附近获取建材和薪材等活动影响高山林线附近森林的结构和功能。 在调查区域,梅里雪山、白马雪山、甲午雪山的林线海拔高度在4200-4300m之间; 四川雅江、理塘一线,林线位置多在4300-4400m;四川壤塘二林场一带的林线主体在4100-4200m,在个别地区达到4300m; 在贡嘎山的南坡和东坡一带,林线位置在3600-3700m;而在四川松潘一带,林线位置主体在3700-3800米左右。树线高度的分布趋势和林线一致。混农季节性放牧及其有关人类利用活动使研究地区很多地方高山林线降低、树线交错带宽变窄或消失。在研究地区,总体情况是,阳坡和半阳坡(南坡、西南坡等)的林线和树线比阴坡和半阴坡(北坡、东北坡等)低,变化幅度达20-200m。这种差异主要是为了开辟牧场而人为清除了南向坡自然林线及其以上的植被从而使林线位置下降所致。在南坡自然林线保留得比较好的地方,林线和树线依然可以达到甚至超过北坡林线和树线的高度。放牧活动抑制了高山林线带火烧迹地的天然更新,从而使林线位置保持在目前的位置。 放牧活动对高山林线带森林群落更新的影响是显著的。自然林线内的乔木个体密度特别是新生苗和幼苗的密度大大高于非自然林线。没有放牧的自然林线及树线交错带内的I级个体(新生苗)密度达到725-2917株/公顷,而与之相对的处理样地内I级个体的密度只有0-228株/公顷;II级个体(高度10-50cm)也表现出类似的趋势,在没有放牧的自然林线及树线交错带样方内,其密度达到550-5208株/,而在放牧处理样方内只有14-321株/公顷。在非自然林线带样地内,在有正常放牧的样地内,完全缺乏I级个体。 从相对比例来看,没有放牧的样方内的I、II级个体在全部个体中所占的比例显著高于有放牧活动的样方。放牧使林线交错带的乔木幼苗数量显著减少,从而影响林线及树线交错带森林群落的天然更新过程。林线和树线交错带的灌木对乔木幼苗具有重要的保护作用,能够为树线树种如冷杉等幼苗的定居体提供有利的微气候环境,同时保护苗免受牲口的啃食和践踏。火烧以后接着进行放牧能够100%地抑制高山林线带的幼苗更新。 高山牧场放牧强度降低、使用时间缩短而低海拔地带放牧强度增加是研究地区混农季节性放牧系统的一个显著变化。这种变化也必然会引起各海拔带上的生态系统的变化。放牧强度的降低、生产性用火的停止将导致原来通过人工火烧而降低并通过进一步的火烧和放牧活动来维持的林线及其以上地带的灌木盖度和高度的增加,从而为林线森林群落的扩张创造条件。 青藏高原东部高山峡谷地区是我国重要的山地生态系统,在我国的生物多样性保护、生态环境建设、社会经济可持续发展战略中具有举足轻重的作用。正确认识人类特别是当地传统的生计系统与生态环境系统的互动关系是实现上述战略目标的前提。决策者必须以综合、系统的的视角协调促进社会经济可持续发展、保护生物及文化多样性和维持人、牲口和生态系统之间的平衡的多重目标。 Agropastoral transhumance, which makes a complementary exploitation of the natural resources at different altitudinal belts through a combination of migratory animal husbandry and crop cultivation, has a long history in Hindu-Kush Himalaya, Tibet Plateau, Hengduan Ranges, eastern and southern Africa and the Andes region of south America.For millennia, this traditional livelihood strategy has been one of the main forms of interaction between human societies inhabiting in these regions and their natural ecocystems. A close co-evolutionary relationship has been developed between this indigenous resources management systems and the mountain vegetation systems on the one hand and a unique set of cultural values and social features on the other. Understanding this relationship has been one of the core scientific issues in mountain ecology and anthropology. In recent years, the importance of the multiple functions of the mountain ecosystems and their dynamic changes in the sustainable socio-economic development of the mountain regions has gained increasing attention. This paper, which is based on a detailed study on the agropastoral practices of the 12 natural villages in Deqin County of Yunnan, and the mountainnn vegetation patterns in Deqin of Yunnan and Rangtang County of Sichuan, intends to reveal the major characteristics, system composition and the inter-relations of the subsystems of the agropastoral transhumance in Eastern Tibetan Plateau as well as the trends of changes of the system within the context of global changes, economic globalization and modernity process of China and analyze the relations between agropastoral transhumance and alpine ecosystem, ao as to understand the interactions between human activities and natural ecosystems of the mountains and provide theoretical basis for the national strategies in eocioeconomic development, environmental reconstruction and biodiversity conservation in the mountain regions. Results of the survey indicate that agropastoral transhumance in the investigated area is a traditional economic form that is highly adapted to the eastern Tibet Plateau where the topography features high peaks and deep gorges and where the highly variable environmental parameters and scanty natural resources exhibit a distinct vertical spectrum of distribution and great temporal and spatial heterogeneity. The main objective of pastoral management is still aimed at the production of basic goods and services of local people and thus the type and size of animals raised for each household mainly depend on local needs and are limited by the availability of natural resources. The scale of production is relatively low. Pastoral resources at different altidudinal belts are complementarily used at different seasons of a year and thus form the resources basis for agropastoral production of the study area. Migration distances and patterns vary with the location of the permanent settlements, the elevational distribution range of the resources of the villages concerned. Natural pastures (rangelands) are the main fodder resources and sumplement feedings only account for less than 5% of the total fodder consumption. Crop cultivation and pastoral activities support each other to form a complete livelihood system. The ability of the farmig lands (crop cultivation) to provide the pastoral activities with concentrates and sumplements often becomes a main factor limiting the scale of livestock production at household level. Agropastoral transhumance is experiencing drastic changes in recent decades as is reflected in the size and composition of animals, the seasonal migration pattern, the relative importance of pastoralism in the household economy and the interplays of agricultural and pastoral elements of the system. In general, there is a decline in animal population and mobility, a shift in animal composition to meet new needs arising from changed macro-economic situation, a decrease in the relative importance in the household economy and an increasing decoupling of agro&pastoral relations. The fundamental divers of these changes can be traced to environmental, social, economic, technological and cultural changes from local to global levels and such changes have further caused local changes in livestock management objectives, land use and distribution of labor forces. Changes in local livelihood systems could have profound political, socioeconomic, cultural and ecological conseuquences. Agropastoral transhumance, as an age-old traditional livelihood strategy, is facing multifacet challenges, such as winter fodder shortage, rangeland degradation, lack of market competitiveness, decrease in economic importance, lack of appreciation among the young generation and adequate policies from the government. At the same time, economic globalization, market economy, intrdoctution of new technologies, increase of alternative income generating opportunities and the national re-oreitation of policies on mountain ecosystems have all brought about new opportunities for the transformation of the traditional livelihood system and the synchronized development of local society and the environment. Agropastoral transhumance interacts with the ecosystems at the timberline and treeline ecotone mainly through the following aspects: 1)Animal browsing and stamping affect the regeneration process of the forest communities and alters the composition and structure of the forest which in turn affect the succession process and vegetation pattern of the forest communities. Forest edges are the priority locations for summer houses and therefore the timeline and treeline area becomes the major venues of aninal activities; (2)herders create, maintain and improve pastures through burning that remove the forest communities at the timeline and treeline ecotone; 3)immediate grazing on the fire sites can significantly prevent the fire sites from perogressive succession; and 4)herders harvesting of construction timber and firewoods affects the structure and functions of the forest communities at the timberline and treeline zone. Timberline position in the survey region shows geographical variations. It is around 4200-4300m in Meilixueshan, Baimaxueshan and Jiawuxueshan in Northwest of Yunnan and rises to 4300-4400m in Yajiang County and Litang County of Sichuan. In Rangtang of Sichuan, it is between 4100-4200m, though reaching 4300m in localized sites. In the southern and eastern slopes of Gongga Mountain, the timberline is only between 3600m and 3700m and in Songpan County at the upper reach of the Minjiang River the timberline is around 3700-3800m.Treeline pattern follows similar trend. In many places, agropastoral transhumance and related human activities have lowered the timberline and treeline and narrowed or removed the treeline ecotone. In the area of survey, generally speaking, timberlines and treelines are lower on the southern slopes than on the northern slopes, with a difference between 20 and 200m. This is mainly because that the use of fires to crerate pastures has removed the forest vegetation at the previous timberline and above. In fact, in many places, well-preserved forests on the south slopes have even high timberline position that the corresponding northern slopes. At subalpine zone, grazing activities could have prohibited the natural regeneration of many forest fire sites and maintained the forest position at the present level. Grazing has a significant impact on the regernation process of forest communities at the timberline zone. Natural timberline and treeline ecotone has much higher density of treeline species individuals especially the emergents and seedlings than the timberlines that are maintained by human activities. In natural timberline and treelien ecotone without grazing interference, the density of the I Class seedlings (less than 10cm in height) ranges 725-2917 /hm2; while that in the treatment plots (with grazing disturbance) is only 0-228//hm2;II Class seedlings (10-50cm)exhibit similar density trends, reaching 550-5208//hm2 in natural timberline without grazing but only 14-321//hm2 in the plots with grazing treatment. In the man-created timberlines, there is no I Class seedling at all in plots with normal grazing activities. In relative terms, in plots without grazing activities, the propotion of I Class and II Class seedlings is much higher than that in plots with grazing. Grazing activities have significantly reduced the number of seedlings in the timberline ane treeline ecotone, and thus affect the natural regeneration process of the forests. Shrubs at the timberline and treeline ecotone can effectively protect the seedlings from severe climate and animal tramping, thus increasing the survival rate of the seedlings. Grazing following fires can completely inhibit forest regeneration process at timberline. Changes in agropastoral transhumance will have great impact on the timberline and treeline pattern of the studied area. The decrease in grazing intensity on alpine pastrues and the cessation of the use of fires will result an increase in the cover and height of shrubs above the present human-maintained treeline, which will create further condition for the expansion of timberline forest communities. Eastern Tibet Plateau harbors some most important mountain ecosystems of China that are of vital importance to the country’s strategy in biodiversity conservation, environmental construction and sustainable sociaoeconomic development. A proper knowledge of the interactions between traditional livelihood systems and the ecosystems in the region is a precondition to the realization of the above strategic goals. Therefore, the decision-makers must have a holistic and systemic perspective so as to integrate the multiple objectives of promoting sustainable socioeconomic development, conserving biological and cultural diversity and maintaining the balances among people, animal population and the ecosystems.

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日益增强的旅游活动干扰正成为九寨沟世界自然遗产的有效保护与持续管理不可回避的挑战,已成为当前区域生态保护与经济发展的焦点问题之一。阐明相关旅游的干扰活动对核心景区植物物种组成、多样性与结构的影响是九寨沟有效保护与持续管理的必要前提和基础,然而至今少有研究。选择九寨沟与旅游活动相关的九个景点、公路建设地段以及退耕地还林地,详细调查了林下植被结构、物种组成与多样性,比较了相关旅游活动干扰与基本未受干扰地段的差异。目的是阐明九寨沟核心景区旅游干扰条件下植物多样性与群落结构及其特点,揭示旅游干扰与植被结构和生物多样的相互关系,评估九寨沟旅游管理的有效性,探索减免旅游干扰影响的对策与措施。初步结论如下。 1.旅游活动引起九寨沟核心景区植物组成和群落结构特征改变显著。栈道及公路附近许多耐荫喜湿的敏感种局部消失,而早熟禾、车前草、委陵菜等耐干旱、耐践踏、繁殖能力强的植物种群扩大;外来物种频繁出现并已少量侵入干扰相对较轻的林分深处;林下群落以草本植物为优势,灌木与苔藓植物的频度、盖度、高度以及灌木密度均有所下降。可见,大量的游人活动和景区公路建设已对九寨沟丰富的乡土植物构成极大威胁,导致其生物多样性降低。而九寨沟农耕地退耕有利于九寨沟生态环境和生物多样性保护,正逐步向稳定群落演替。 2.干扰强度的差异导致九寨沟植物所受影响的程度不同。栈道附近植物受影响的程度与游人活动频率有关,活动频率高的地段干扰强度大,对植物的影响程度重,反之植物所受的影响较轻。强度干扰地段,耐荫喜湿的物种少见,伴生植物优势地位突出,生物多样性明显降低,植物群落结构特征改变也极为显著;干扰较轻的地段,伴生物种少量出现,植物群落结构变化不明显,生物多样性略为降低,部分地区结构参数值和多样性指数有所升高。公路修建是一种强度干扰,它导致附近植物种类极为单一,草本优势种异常突出,多数植物生活力低下、生长更新能力差。 3.不同植物类群受干扰影响的程度不同。草本及苔藓植物的种类组成和多样性指数受干扰影响较大,灌木和苔藓植物的结构受干扰影响较大,苔藓植物对干扰影响最为敏感。 综合分析表明,九寨沟核心景区的管理虽然比较规范,但目前核心区热点景点段的管理仍然不够,旅游活动驱动了林下植被退化明显、物种组成显著变化、生物多样性衰退、非乡土喜光耐旱种群扩大。导致九寨沟核心区旅游活动与生物多样性保护目标尖锐冲突,进一步约束旅游活动带来的干扰,强化管理,开展林下植被恢复与非乡土喜光耐旱种群调控是九寨沟自然遗产地保护一项紧迫任务。 The increasing tourism disturbance is an unavoidable challenge to effective conservation and sustained tourist management of Jiuzhaigou Nature Reserve. It has become one of the focal problems of regional ecological protect and economic development. It is important to clarify effects of tourism disturbance on plant species composition, diversity and community structure in kernel spot for effective conservation and sustained tourist management in Jiuzhaigou, China. However, there were little studies about this yet. The study investigated the vegetation structure, species composition and diversity at nine sight spot, road area and four abandoned farmlands connecting with tourism, and compared the differences between disturbed area and undisturbed area. The purpose of the this study is clarifying the plant diversity and community structure and characteristics of the disturbed area in kernel spot of Jiuzhaigou, discovering the relation between vegetation structure and biodiversity, evaluating the effect of tourist management and exploring the measure decreasing tourist disturbance. Our results are following: 1. Tourism disturbance caused a significant change in species composition and structure of plant communities in kernel Spot of Jiuzhaigou. In the vicinity of plank and road, some native shade-tolerant or hygrophilous plants had disappeared, accompanying with the population expansion of some xerophilous and disturbance-resisting species such as Poa sp. Plantago depressa, Potentiila multicaulis and some exotic and synanthropic species. Herbs were domaint species, while frequency coverage and height of shrubs and bryophytes, and shrub density decreased. In indicated that tourist activities and build of road had adversely affected on native plant species, and led to decline in biodiversity of Jiuzhaigou Nature Reserve. Abandoned farmlands maybe conduced to entironment and biodiversity conservation. 2. Nearby the plank, influency variable of tourism disturbance on plant was alosely and positively correlated with disturbance intensity. There was companion plant species and lacked shape-loving species in heavy disturbed areas, which caused decrease in biodiversity and significant change in community structure in these places. On the contrary, in the slightly disturbed areas, some companion speices displayed and biodiversity decreased slightly, but no significant change in community structure in these areas. The biuld of road is a heavy disturbance form, which led to increase of herb species and to decrease in vitality and regeneration capacity. 3. The intensity of tourism disturbance on plant depended on plant groups. Tourism interference significantly influenced species composition and diversity index of herb and bryophyte; it also significantly influenced community structure; the bryophyte was more sensitive to tourism disturbance. Our result indicated that the management in kernel spot of Jiuzhaigou is relatively reasonable, but not adequate. Tourism speed the degradation of the vegetation under woodland, the change of the species composition, the decreaing of the biodiversity and the expanding of the exotic sunloving plant populations. The contradiction between tourism and conserving biodiversity is increasing, so enforcing management, regenerating the vegetation under woodland and adjusting the exotic sunloving arid-tolerent plant populations is a pressing work to protect the Jiuzhaigou natural legacy.

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本文以青藏高原东部的高山草甸为研究对象,设置早融、中间及晚融三个融雪部位,采用实验室测量、野外测量、野外样方调查相结合的 方法,从个体、种群和群落的水平上比较研究了高山雪场植物在同一雪场样地中不同融雪梯度上的特征变异及适应,结果表明: 从早融到晚融的梯度上,随着融雪时间的逐渐推迟,表土日温差降低,冻融交替的强度减弱,土壤水份逐渐增加,总N、总P、总K 以及 可溶性的N、P 和pH 变化不明显,土壤有机质及可溶性的K 和Ca 逐渐降低。冻融交替强度上的差异以及土壤水分差异被认为是融雪梯度上 影响植物生长的主要原因。 从早融到晚融的梯度上,伴随着生态因子的改变,几种常见植物的个体特征也发生相应的变化。首先,物候期推迟。植物开始生长的时间 一般要推迟将近二十天,但同一种植物在不同的融雪部位上的衰老期趋于一致,这预示着在晚融部位同一植物的生长期要缩短。其次,个体生 长特性发生改变。黑褐穗苔草(Carex atrofusca subsp. minor (Boott) T.Koyama)和西北黄芪(Astragalus fenzelianus Pet.-Stib.)的个体生长(株高、单株叶数、单叶面积和地上生物量)表现为逐渐增加的趋势;斑唇马先蒿(Pedicularis longiflora Rudolph var. tubiformis (Klotz.) Tsoong)和川西小黄菊(Pyrethrum tatsienense (Bur. et Franch.) Ling ex Shih.)则表现为逐渐降低的趋势;长叶火绒草(Leontopodium longifolium Ling)在融雪梯度上的变化趋势不明显。再次,从繁殖特性来看,大卫马先蒿(Pedicularis davidii var. pentodon Tsoong)的单株花数、单花种子数、种子千粒重及种子萌发率随融雪的推迟呈现为逐渐增加的趋势;圆穗蓼(Polygonum macrophyllum D.Don)的种子(小坚果)千粒重和萌发率也表现为逐渐增加,其余繁殖特征变化不明显。 在种群层次上,几个常见物种的分布格局随着融雪的推迟都发生一定的变化,基本上表现为从早融的集群分布到中间或晚融部位的随机分布。物种间的联结性也发生较大的变化,由早融部位的总体上的正关联逐步过度到晚融部位上的总体上的负关联。特定种对间的联结性也发生较大的变化。恶劣环境条件(如剧烈的冻融交替)的影响以及对恶劣条件适应被认为是分布格局及种间联结性发生变化的主要原因。 在群落层次上,物种多样性的变化表现为单峰曲线的格局,即在中间部位多样性最高。早融部位强烈的冻融交替和晚融部位缩短的生长季是早融及晚融部位物种多样性不高的重要原因。几乎所有的只出现在一个融雪部位(雪深级别)上的物种都发生在中间融雪部位。这说明,中等的雪深更有利于许多高山植物的存活,而过浅过深的积雪都不利于植物的生存。另外,相距较近的融雪梯度之间的物种相似性较大,而相距较远的梯度之间物种的替代率较高,物种的相似性较小。在群落的生物量方面,地上生物量随融雪的推迟而升高,地下生物量随融雪的推迟而下降,地上与地下生物量之总和随着融雪的推迟而下降,地下生物量与地上生物量之比随着融雪的推迟而下降。早融部位的地上生物量主要集中于地上0-10cm 的范围内,表明在早融部位植物地上部分有变矮的趋势;早融部位的地下生物量在土壤各深度分布相对较均一,而晚融部位地下生物量则主要集中于地下0-10cm 的范围内。生物量的变化趋势主要与雪场中各部位的土壤水分含量及地表日温度差异有关,是植物适应特定环境的结果。 To detect the plants’ responses to snow-cover gradients in an alpine meadow of eastern Tibetan plateau, laboratory method and field sample plot method were employed, and three gradeients (early-, medium and late-melting)were established in a natural snowbed. The measurements were carried out for two years and was done on three levels——individual, population and community. The results are shown as follows : From early- to late-melting gradients, daily ground temperature difference between day and night decreased, amplitude of freeze-thaw alternation weakened, soil organic matter contents and soluble K and Ca decreased, while soil water content increased. Total N, total P, total K,pH soluble N and soluble P kept constant from early- to late-melting portions. Among these factors, the changes of intense freeze-thaw alternation and soil water contents were considered as main factors affecting plants’ growth. From early- to late-melting portions, all phenological phases postponed, e.g. phase of plant emergence postponed almost twenty days. However, the same species’ individuals at different portions withered in step, which implied that the individuals at late-melting portion possessed shorter growing season length. Along the same gradient, both Carex atrofusca subsp. minor (Boott) T. Koyama and Astragalus fenzelianus Pet.-Stib. increased their individual growth, whereas Pedicularis longiflora Rudolph var. tubiformis (Klotz.) Tsoong and Pyrethrum tatsienense (Bur. et Franch.) Ling ex Shih. decreased their individual growth. Unlike the four plants mentioned above, Leontopodium longifolium L. did not show any evident change. As to reproductive charateristics, the flowers per individual, the number of seeds per flower, the thousand seed weight and the seed germination rate of Pedicularis davidii var. pentodon showed an increasing trend; and Polygonum macrophyllum D.Don also increased its thousand seed weight and seed germination rate along the same gradient. However, the other reproductive charateristics of Polygonum macrophyllum D.Don did not change significantly. At population level, the distribution pattern of several selected species changed from cluster pattern to random pattern as the snowmelt postponed. Overall association among the species changed from positive to negative along the same gradient. Further, interspecific association also changed evidently. Adverse circumstances such as intense freeze-thaw alternation were considered as primary factors resulting in changes of population distribution pattern and interspecific association. At the level of community, species diversity showed a pattern of a unimodal trend, i.e. the highest diversity occurred at medium snow depth,perhaps because of intense freeze-thaw alternation at early-melting portions and the shortest growing season at late-melting portions. Almost all species that only appeared at one snowmelt portion occurred at medium portion, indicating that medium snow depth was more suitable for many species’ survival. Species replacement from one snowmelt portion to its neighboring portion seldom took place. However, while distance between two portions became farther, species replacement between the two portions occurred more frequently. As for biomass, aboveground biomass increased from early- to late-melting portions, whereas belowground biomass, total biomass and the ratio of belowground to aboveground all decreased along the same snow gradient. A majority of aboveground biomass distributed in a height range of 0-10 cm, suggesting that height of plants inhabiting early-melting portion be shorter compared with other portions. In addition, belowground biomass at early-melting portion was evenly distributed at different soil depth in comparison with aboveground biomass, whereas belowground biomass at late-melting portion concentrated 0-10cm soil layer below ground. The changing trend of biomass was also related to two factors. One was soil water content, and the other topsoil temperature difference between day and night.

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黄龙世界自然遗产地岷江冷杉林(Abies faxoniana)生境类型多样,群落结构复杂,群落植物种类组成多样性丰富。揭示不同生境的生物多样性及其差异是认识生物多样性格局、形成及维持机制的前提和进行多样性保育的基础。本文采用样方法对黄龙钙化滩生境、阴坡非钙化生境及半阳坡非钙化生境的岷江冷杉原始林植物群落结构及植物多样性进行了研究。结果表明: 黄龙岷江冷杉林具有明显的复层异龄结构,垂直结构明显,乔木、灌木、草本、苔藓层次分明。共发现高等植物386 种,其中维管植物46 科103 属163 种,苔藓植38 科83 属物223 种。各层片结构及物种组成如下: (1)钙化滩生境、阴坡非钙化生境、半阳坡非钙化生境分别发现乔木18 种、13种、8 种。乔木层均可分为两个亚层,第一亚层优势种均为岷江冷杉,第二亚层主要为岷江冷杉异龄树或其它大高位芽物种。钙化滩生境第一亚层除优势种岷江冷杉外混生有巴山冷杉(Abies fargesii)、粗枝云杉(Picea asperata)以及阔叶树种白桦(Betula platyphylla)等,第二亚层主要为岷江冷杉异龄树;阴坡非钙化生境第一亚层除优势种岷江冷杉外间有巴山冷杉和白桦,第二亚层物种主要为川滇长尾槭(Acer caudatum var. prattii);半阳坡非钙化生境第一亚层除优势种岷江冷杉外混生有巴山冷杉,第二亚层主要为岷江冷杉异龄树。依乔木层优势种的差异,钙化滩生境及半阳坡非钙化生境为岷江冷杉纯林,阴坡非钙化生境为岷江冷杉-川滇长尾槭混交林。不同生境乔木层郁闭度、乔木密度、树高结构、直径结构均存在差异。 (2)钙化滩生境发现灌木41 种,平均盖度为18.49±1.72(%),平均高度为52.12±4.45(cm),优势种为直穗小檗(Berberis dasystachya);阴坡非钙化生境发现灌木30 种,平均盖度为29.33±2.56 (%),平均高度为119.55±8.01 (cm),优势种为箭竹 (Fargesia spathacea) 、唐古特忍冬(Lonicera tangutica) 和袋花忍冬(Lonicera saccata);半阳坡非钙化生境发现灌木29 种,平均盖度为31.35±1.93 (%),平均高度为107.55±4.24 (cm),优势种为箭竹(Fargesia spathacea)。不同生境灌木层结构和物种组成多样性差异显著,钙化滩生境的灌木盖度、高度总体上较非钙化的坡地生境低, 钙化滩生境灌木以小型叶的落叶灌木为主,沟两侧非钙化的坡地生境上则发育了丰富箭竹。 (3)钙化滩生境发现草本46 种,平均盖度为7.18±0.79 (%),平均高度为5.04±0.26(cm),以山酢浆草(Oxalis griffithii)为优势种;阴坡非钙化生境发现草本物种71 种,平均盖度达29.04±2.31(%),平均高度为9.08±0.52(cm),以钝叶楼梯草(Elatostema obtusum)、山酢浆草为优势种;半阳坡非钙化生境草本物种50 种,平均盖度为以8.79±0.82(%),平均高度为7.67±0.43 (cm),以扇叶铁线蕨(Adiantum flabellulatum)、双花堇菜(Viola biflora)、华中蛾眉蕨(Lunathyrium shennongense)、山酢浆草为优势种。阴坡非钙化生境草本层片发育良好,多样性最为丰富,盖度和物种丰富度均显著高于钙化滩生境和半阳坡非钙化生境。 (4)钙化滩生境发现苔藓物种140 种,平均盖度达84.25±1.30 (%),以仰叶星塔藓(Hylocomiastrum umbratum) 等大型藓类为优势种;阴坡非钙化生境发现苔藓物种115 种,平均盖度为79.29±1.64 (%),以刺叶提灯藓(Mnium spinosum)、大羽藓(Thuidium cymbifolium)、毛尖燕尾藓(Bryhnia trichomitra)等个体较小的物种为优势种;半阳坡非钙化生境发现苔藓物种91 种,平均盖度为60.64±1.93 (%),也以刺叶提灯藓为优势种。 (5)钙化滩生境、阴坡非钙化生境、半阳坡非钙化生境的物种数分别为234 种、221 种、175 种。乔木层的Shannon-Wiener 指数分别为0.75 ±0.12、1.87±0.12、1.78±0.07(灌木层,0.44±0.08、1.71± 0.15、2.49±0.06;草本层,0.33±0.13、1.31±0.15 、2.15±0.08; 苔藓层1.30±0.11、2.08±0.04、1.73±0.11,);Pielou 均匀度指数分别为0.45±0.05、0.29±0.06、0.28±0.08(灌木层,0.75±0.03、0.68±0.05、0.52±0.06;草本层,0.68±0.02、0.77±0.02、0.74±0.02;苔藓层,0.40±0.03、0.63±0.02、0.52±0.03);Simpson's 优势度指数分别为0.63±0.06、0.78±0.04、0.83±0.07(灌木层,0.21±0.03、0.28±0.05、0.45±0.06;草本层,0.25±0.02、0.12±0.01、0.17±0.01;苔藓层,0.45±0.04、0.18±0.01、0.31±0.04)。三种生境间乔木层、草本层的Sorenson 群落相似性系数较低, 灌木层、苔藓层的的Sorenson 群落相似性系数较高。 综上所述,黄龙岷江冷杉林的群落结构、植物多样性在三种生境间存在差异性,这将意味着我们在进行黄龙世界自然遗产地的森林经营管理时要较多地关注岷江冷山林群落在不同生境中的差异性。 There were multiplex habitat types, complicated community structure and abundant species composition in the Huanglong World Natural Heritage Site. Uncovering the differences of biodiversity among different habitats was a precondition to understand the distribution, formation and sustaining mechanism of the biodiversity, and the foundation of biodiversity conservation. In the present study, using plenty of quadrants, we investigated the community structure and the biodiversity of the primitive Abies faxoniana forest in different habitats (travertine bottomland, semi-sunny-slope non-calcified habitat and shady-slope non-calcified habitat) in the Huanglong World Natural Heritage Site. The main results are as follows: All the primitive Abies faxoniana forests in the three habitats were uneven-aged with obvious vertical structure including tree layer, shrub layer, herb layer and bryophyte layer. A total of 386 higher plants including 163 vascular plant species (103 generic, 46 families) and 223 bryophyte species (83 generic, 38 families) were investigated. The structure and species composition of each layer are as follows: (1) There were 18, 13 and 8 tree species in travertine bottomland, shady-slope non-calcified habitat and semi-sunny-slope non-calcified habitat, respectively. The tree layers in all habitats can be divided into two clear sub-layers. The upper tree layers were dominated by Abies faxoniana, and the lower tree layers were dominated by uneven-aged Abies faxoniana or other phanerophytes species. There were Abies fargesii , Picea asperata and Betula platyphylla besides the dominated species (Abies faxoniana) in the upper tree layer in travertine bottomland, and the lower tree layers were dominated by uneven-aged Abies faxoniana; There were Abies fargesii and Betula platyphylla besides the dominated species (Abies faxoniana) in the upper tree layer in shady-slope non-calcified habitat, and the lower tree layers were dominated by Acer caudatum var. prattii; There was Abies fargesii besides the dominated species (Abies faxoniana) in the upper tree layer semi-sunny-slope non-calcified habitat, and the lower tree layers were dominated by uneven-aged Abies faxoniana. According to composition percentage of dominate species in tree layer, both the forest in travertine bottomland and in semi-sunny-slope non-calcified habitat could be ranked as pure forest, and the forest in shady-slope non-calcified habitat could be ranked as mingled forest. There were significant differences in crown density, plant density, height structure and diameter structure among the three habitats. (2) A total of 41 shrub species (average coverage 18.49±1.72%; average height 52.12±4.45 ㎝)were found in travertine bottomland, and the dominate species was Berberis dasystachya; A total of 30 shrub species (average coverage 29.33±2.56 %;average height 119.55±8.01 ㎝)were found in shady-slope non-calcified habitat, and the dominate species was Fargesia spathacea, Lonicera tangutica and Lonicera saccata. A total of 29 shrub species (average coverage 31.35±1.93%; average height 107.55±4.24 ㎝) were found in semi-sunny-slope non-calcified habitat, and the dominate species was Fargesia spathacea. There were significant differences in structure and species diversity of the shrub layers among the three habitats. The coverage and height of shrub had lower value in travertine bottomland than in two non-calcified habitats. Moreover, travertine bottomland was dominated by deciduous shrub species with microphyll and non-calcified habitats developed abundant Fargesia spathacea species. (3) A total of 46 herb species (average coverage 7.18±0.79%;average height 5.04±0.26 ㎝)were found in travertine bottomland, and the dominate species was Oxalis griffithii; A total of 71 herb species (average coverage 29.04±2.31%;average height 9.08±0.52 ㎝)were found in shady-slope non-calcified habitat, and the dominate species was Elatostema obtusum and Oxalis griffithii. A total of 50 herb species (average coverage 8.79±0.82%;average height 7.67±0.43 ㎝) were found in semi-sunny-slope non-calcified habitat, and the dominate species was Adiantum flabellulatum, Viola biflora, Lunathyrium shennongense and Oxalis griffithii. Herb layers developed well in shady-slope non-calcified habitat and had the higher species richness and coverage than travertine bottomland and semi-sunny-slope non-calcified habitat. (4) A total of 140 bryophyte species (average coverage 84.25±1.30%)were found in travertine bottomland, and the dominate species was big bryophyte species such as Hylocomiastrum umbratum and so on; A total of 115 bryophyte species (average coverage 79.29±1.64%)were found in shady-slope non-calcified habitat, and the dominate species was small bryophyte species such as Mnium spinosum, Thuidium cymbifolium, Bryhnia trichomitra and so on. A total of 91 bryophyte species (average coverage 60.64±1.93%) were found in semi-sunny-slope non-calcified habitat, and the dominate species was Mnium spinosum. (5) There were 234, 221 and 175 plant species in travertine bottomland, shady-slope non-calcified habitat and semi-sunny-slope non-calcified habitat, respectively. Shannon-Wiener index of the tree layer was 0.75 ±0.12, 1.87±0.12 and 1.78±0.07 (the shrub layer, 0.44±0.08, 1.71± 0.15 and 2.49±0.06; the herb layer, 0.33±0.13, 1.31±0.15 and 2.15±0.08; the bryophyte layer, 1.30±0.11, 2.08±0.04 and 1.73±0.11.) for the three habitats, respectively; Pielou index of the tree layer was 0.45±0.05, 0.29±0.06 and 0.28±0.08 (the shrub layer, 0.75±0.03, 0.68±0.05 and 0.52±0.06; the herb layer, 0.68±0.02, 0.77±0.02 and 0.74±0.02; the bryophyte layer, 0.40±0.03, 0.63±0.02 and 0.52±0.03.) for the three habitats, respectively. Simpson's index of the tree layer was 0.63±0.06, 0.78±0.04 and 0.83±0.07 (the shrub layer, 0.21±0.03、0.28±0.05、0.45±0.06; the herb layer, 0.25±0.02, 0.12±0.01 and 0.17±0.01; the bryophyte layer, 0.45±0.04, 0.18±0.01 and 0.31±0.04.) for the three habitats, respectively. There were low Sorenson index both in the tree layer and in the herb layer among the three habitats, whereas, high Sorenson index occurred both in the shrub layer and in the bryophyte layer. To sum up, there were differences both in community structure and plant diversity among the three different habitats, which means that we should pay more attention to habitats heterogeneities of the primitive Abies faxoniana forest when we take action to manage the forest in the Huanglong World Natural Heritage Site.

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小麦条锈病(Puccinia striiformis f. sp. tritici)是世界性小麦病害,可导致受害小麦减产30%以上,甚至绝收。小麦条锈病在我国西南、华北麦区危害严重,四川麦区是小麦条锈病发病最重的地区之一,每年因条锈病流行造成小麦产量损失巨大。利用抗条锈病品种是控制该病害最安全、经济的有效途径,因此挖掘利用抗病新基因,开展抗病遗传基础研究是当前育种工作中面临的重要任务。 偏凸山羊草(Aegilops ventricosa,DDMvMv,2n=28)是一年生草本植物,起源于地中海西部沿岸地区,具有对小麦白粉病、锈病等高抗或免疫、耐盐、抗寒、蛋白质含量高等优良性状,是小麦遗传育种很好的种质资源。本研究以高抗条锈病的小麦—偏凸山羊草6Mv/6B代换系(Moisson 6Mv/6B)为材料,对其含有的带条锈病抗性基因的偏凸山羊草6Mv染色体在四川小麦背景中的传递情况、与小麦—簇毛麦双端体附加系所具有的白粉病抗性的聚合以及对Moisson 6Mv/6B进行电离辐射诱变筛选抗条锈病的小麦—偏凸山羊草易位系三个方面进行了研究。取得的主要研究结果如下: 1. Moisson 6Mv/6B与高感条锈病的四川地区普通小麦品种绵阳26、绵阳93-124和SW3243的杂种F1与其普通小麦亲本分别作为父、母本回交,通过对其BC1和F2的结实率、根尖细胞有丝分裂中期染色体的观察以及对条锈病抗性的鉴定,发现含6Mv染色体的F1植株作母本时的回交结实率(83.10%)普遍高于含6Mv染色体的F1植株作父本(48.61%),结实率与普通小麦基因型密切相关(χ2=34.15>>χ20.05=5.99(df=2));6Mv染色体在三种四川小麦中通过雌、雄配子传递的传递方式与其传递率间没有显著相关性,其传递率与普通小麦基因型呈显著相关性(χ2=6.42>χ20.05=5.99(df=2))。 2. Moisson 6Mv/6B与高抗白粉病的小麦—簇毛麦双端体附加系Pana(2n=42+2t)正反杂交,希望在聚合两者抗性的同时观察不同受体背景下的抗性反应。对Moisson 6Mv/6B和Pana正反杂交的结实率、杂交后代的农艺性状进行观察,并对杂交后代进行基因组荧光原位杂交(GISH)分析及条锈病和白粉病的抗性鉴定。结果表明Moisson 6Mv/6B作母本时杂交结实率(80.56%)高于Pana作母本时(58.33%),结实率与杂交方式间紧密相关(χ2=4.96>χ20.05=3.84(df=1));Moisson 6Mv/6B和Pana杂交后代株高比最高亲本高约10cm,成熟期也较两亲本提前两个星期左右;正反杂交后代中具有偏凸山羊草6Mv染色体的植株具有条锈病抗性,具有簇毛麦端体的植株具有白粉病抗性,同时筛选到4株含有偏凸山羊草和簇毛麦遗传物质并对条锈病和白粉病兼抗的材料,证明来自偏凸山羊草6Mv染色体的条锈病抗性与来自簇毛麦端体的白粉病抗性已经聚合在一起,且没有产生相互抑制的作用,暗示通过这两个抗性基因的聚合是完全能获得兼抗条锈病和白粉病的小麦新种质。 3. 对Moisson 6Mv/6B在减数分裂时期的成株进行总剂量为6Gy、辐射频率为120rad/min的60Co-γ射线辐射,对辐射植株自交后代进行农艺性状及根尖细胞有丝分裂中期染色体形态观察和条锈病抗性鉴定。结果为辐射植株自交结实率为2.22%,根尖细胞有丝分裂中期的染色体存在明显碎片,辐射自交后代植株对条锈病具有成株期抗性。 小麦—偏凸山羊草6Mv/6B代换系对条锈病抗性稳定,是培育条锈病抗性品种的良好供体。本研究证明在四川小麦背景中要利用该品种抗性,在结实数满足需要时,可将其作父本,亦可作母本,但关键是要选择好一个优良的受体基因型;同时其条锈病抗性与来自簇毛麦的白粉病抗性没有相互抑制作用,可将两者抗性有效聚合用于小麦育种中。 Wheat stripe rust (Puccinia striiformis f. sp. Tritici) is a worldwide disease of wheat, and could lead to victims of 30 percent or even total destruction of wheat production. Wheat stripe rust harms badly in China's southwest and North China. Sichuan province is one of the regions damaged by wheat stripe rust heavily. The use of resistant varieties is the most secure and economical way to control the wheat stripe rust. Therefore, it is essential to identify new disease-resistant genes and genetically research of disease resistance. Aegilops ventricosa (DDMvMv, 2n = 28) is an annual herbaceous plant, originating in the coastal areas of the western Mediterranean, with good characters such as resistance of wheat powdery, rust, salt, cold and high protein content. It is a good germplasm resource. In this study, the wheat- Aegilops ventricosa 6Mv/6B substitution line Moisson 6Mv/6B (highly resistant to the wheat stripe rust) was used to study on the transmission of chromosome 6Mv of Aegilops ventricosa in different genetic background of Sichuan wheat varieties, hybridization with wheat- Haynaldia villosa ditelosomic addition line Pana (highly resistant to the powdery mildew) and screening of wheat- Aegilops ventricosa translocation line by exposuring Moisson 6Mv/6B under ionizing radiation. The main results are as following: 1. Moisson 6Mv/6B was crossed with Sichuan wheat varieties mianyang26, mianyang93-124 and SW3243 (highly susceptible to stripe rust), respectively. Their F1 hybrids were further backcrossed as male and female to corresponding wheat varieties. The seed-setting rate, chromosomes confirmation in the mitotic metaphase of root tip cells, and resistance to stripe rust of the subsequent BC1 and F2 plants were investigated. The average seed-setting rate of backcross via 6Mv as female donor (83.10%) was higher than that of backcross via 6Mv as male donor (48.61%), suggesting that the seed-setting rate was associated with the wheat genotypes(χ2=34.15>>χ20.05=5.99(df=2)). In all analyzed populations, transmission frequencies of chromosome 6Mv were not correlated with the ways of 6Mv through male or through female. However, transmission frequencies of chromosome 6Mv were significantly correlated with Sichuan wheat genotypes(χ2=6.42>χ20.05=5.99(df=2)). 2. To aggregating the resistances to stripe rust and powdery mildew, as well as research on the resistance reactions in different genetic background, Moisson 6Mv/6B was reciprocally hybrided with the wheat- Haynaldia villosa ditelosomic addition line Pana (highly resistant to the powdery mildew). The seed-setting rate, agronomic characters, genomic in situ hybridization (GISH) of hybrid progenies,and resistances to stripe rust and powdery mildew were investigated. The results showed that the seed-setting rate of hybridization via Moisson 6Mv/6B as female donor (80.56%) was significant higher than that via Pana as female donor (58.33%). The seed-setting rate was associated with the hybrid methods (χ2 = 4.96> χ20.05 = 3.84 (df = 1)). The plant height of hybrid progenies was about 10 cm higher than Pana, the parent with maximum height. And the maturity of hybrid progenies was about two weeks earlier than that of the parents. In the hybrid progenies, the plants with the 6Mv chromosome have the resistance to stripe rust and the plants with the telosome from Haynaldia villosa have the resistance to powdery mildew. It was found that four plants with both the 6Mv chromosome and the telosome from Haynaldia villosa were resistant to stripe rust and powdery mildew. It indicated that the resistance to stripe rust and powdery mildew aggregated, and no mutual inhibition was found. It implied that the aggregation of the two resistance genes was able to provide the new wheat germplasm with the resistances to stripe rust and powdery mildew. 3. Moisson 6Mv/6B was irradiated with 60Co-γ rays of 6Gy (120rad/min) during meiosis. The agronomic characters and chromosomes confirmation in the mitotic metaphase of root tip cells,as well as resistance to stripe rust were investigated. The seed-setting rate of irradiated plants was only 2.22%. The chromosomes in mitotic metaphase had clear fragments. The resistance to stripe rust of progeny of irradiated plants was the adult-plant resistance. The wheat- Aegilops ventricosa 6Mv/6B substitution line is a good stripe rust resistance donor for its stabile resistance. Our study demonstrated that the key for use the resistance is to choose a good receptor. There is no difference between Moisson 6Mv/6B be the female and be the male if the seed number meets the requirement. At the same time, the stripe rust resistance of Moisson 6Mv/6B did not have the mutual inhibition with the powdery mildew resistance from Haynaldia villosa. It is able to aggregate the two resistances for wheat breeding.