Linear-to-Circular Polarization Reflector With Transmission Band

A new class of polarizing surface is proposed that in a given frequency band can reflect incident linearly polarized waves with circular polarization (CP) while at other frequencies is transparent allowing incident waves to transmit unaffected. The proposed structure consists of two parallel anisotropic frequency selective surfaces (FSSs) that independently interact with TE or TM waves, respectively. The FSSs are designed to, respectively, transmit TE and TM waves within the same transmission frequency range, so that the combined structure is transparent to all polarizations in this band. Likewise, the two arrays are designed to, respectively, reflect TE and TM incident waves in a common reflection band, so that all polarizations are fully reflected in this range; if the separation of the two arrays is such that the TE and TM components of an incident wave polarized at slant 45° experience a 90° phase shift, reflection will occur in CP. The concept and performance limitations are theoretically investigated using transmission line theory as well as full wave results. The predicted performance is validated by means of experimental results on a fabricated prototype. The proposed structure is pertinent for employment as a quasi-optical diplexer in CP dual-band systems such as reflector antennas.

Mutant p53 expression in fallopian tube epithelium drives cell migration

Ovarian cancer is the fifth leading cause of cancer death among US women. Evidence supports the hypothesis that high-grade serous ovarian cancers (HGSC) may originate in the distal end of the fallopian tube. Although a heterogeneous disease, 96% of HGSC contain mutations in p53. In addition, the "p53 signature," or overexpression of p53 protein (usually associated with mutation), is a potential precursor lesion of fallopian tube derived HGSC suggesting an essential role for p53 mutation in early serous tumorigenesis. To further clarify p53-mutation dependent effects on cells, murine oviductal epithelial cells (MOE) were stably transfected with a construct encoding for the R273H DNA binding domain mutation in p53, the most common mutation in HGSC. Mutation in p53 was not sufficient to transform MOE cells but did significantly increase cell migration. A similar p53 mutation in murine ovarian surface epithelium (MOSE), another potential progenitor cell for serous cancer, was not sufficient to transform the cells nor change migration suggesting tissue specific effects of p53 mutation. Microarray data confirmed expression changes of pro-migratory genes in p53(R273H) MOE compared to parental cells, which could be reversed by suppressing Slug expression. Combining p53(R273H) with KRAS(G12V) activation caused transformation of MOE into high-grade sarcomatoid carcinoma when xenografted into nude mice. Elucidating the specific role of p53(R273H) in the fallopian tube will improve understanding of changes at the earliest stage of transformation. This information can help develop chemopreventative strategies to prevent the accumulation of additional mutations and reverse progression of the "p53 signature" thereby, improving survival rates.

Tumorigenesis and peritoneal colonization from fallopian tube epithelium

Ovarian cancer is the most lethal gynecological malignancy, primarily because its origin and initiation factors are unknown. A secretory murine oviductal epithelial (MOE) model was generated to address the hypothesis that the fallopian tube is an origin for high-grade serous cancer. MOE cells were stably altered to express mutation in p53, silence PTEN, activate AKT, and amplify KRAS alone and in combination, to define if this cell type gives rise to tumors and what genetic alterations are required to drive malignancy. Cell lines were characterized in vitro and allografted into mice. Silencing PTEN formed high-grade carcinoma with wide spread tumor explants including metastasis into the ovary. Addition of p53 mutation to PTEN silencing did not enhance this phenotype, whereas addition of KRAS mutation reduced survival. Interestingly, PTEN silencing and KRAS mutation originating from ovarian surface epithelium generated endometrioid carcinoma, suggesting that different cellular origins with identical genetic manipulations can give rise to distinct cancer histotypes. Defining the roles of specific signaling modifications in tumorigenesis from the fallopian tube/oviduct is essential for early detection and development of targeted therapeutics. Further, syngeneic MOE allografts provide an ideal model for pre-clinical testing in an in vivo environment with an intact immune system.

Three-dimensional modeling of the human fallopian tube fimbriae

OBJECTIVE: Ovarian cancer is the most lethal gynecological malignancy that affects women. Recent data suggests that the disease may originate in the fallopian fimbriae; however, the anatomical origin of ovarian carcinogenesis remains unclear. This is largely driven by our lack of knowledge regarding the structure and function of normal fimbriae and the relative paucity of models that accurately recapitulate the in vivo fallopian tube. Therefore, a human three-dimensional (3D) culture system was developed to examine the role of the fallopian fimbriae in serous tumorigenesis.

METHODS: Alginate matrix was utilized to support human fallopian fimbriae ex vivo. Fimbriae were cultured with factors hypothesized to contribute to carcinogenesis, namely; H2O2 (1mM) a mimetic of oxidative stress, insulin (5μg/ml) to stimulate glycolysis, and estradiol (E2, 10nM) which peaks before ovulation. Cultures were evaluated for changes in proliferation and p53 expression, criteria utilized to identify potential precursor lesions. Further, secretory factors were assessed after treatment with E2 to identify if steroid signaling induces a pro-tumorigenic microenvironment.

RESULTS: 3D fimbriae cultures maintained normal tissue architecture up to 7days, retaining both epithelial subtypes. Treatment of cultures with H2O2 or insulin significantly induced proliferation. However, p53 stabilization was unaffected by any particular treatment, although it was induced by ex vivo culturing. Moreover, E2-alone treatment significantly induced its canonical target PR and expression of IL8, a factor linked to poor outcome.

CONCLUSIONS: 3D alginate cultures of human fallopian fimbriae provide an important microphysiological model, which can be further utilized to investigate serous tumorigenesis originating from the fallopian tube.

An analytical solution for solid stresses in a silo with an inner tube

This paper presents an analytical solution for the solid stresses in a silo with an internal tube. The research was conducted to support the design of a group of full scale silos with large inner concrete tubes. The silos were blasted and formed out of solid rock underground for storing iron ore pellets. Each of these silos is 40m in diameter and has a 10m diameter concrete tube with five levels of openings constructed at the centre of each rock silo. A large scale model was constructed to investigate the stress regime for the stored pellets and to evaluate the solids flow pattern and the loading on the concrete tube. This paper focuses on the development of an analytical solution for stresses in the iron ore pellets in the silo and the effect of the central tube on the stress regimes. The solution is verified using finite element analysis before being applied to analyse stresses in the solid in the full scale silo and the effect of the size of the tube.

In-Situ, Simultaneous Irradiation and Monitoring of a Photocatalysed Organic Oxidation Reaction in a TiO2-coated NMR Tube

The semiconductor photocatalysed (SPC) oxidation of toluene is performed inside an NMR spectrometer and the reaction monitored simultaneously in-situ, using a fibre optic probe/diffuser to provide the UV light to activate the titania photocatalyst coating on the inside of the NMR tube. Such a system has great potential for the simple rapid screening of a wide range of SPC mediated organic reactions.

Circular polarisation frequency selective surface operating in Ku and Ka band

A double layer circular polarization (CP) frequency selective surface (FSS) for use as a dual-band quasi-optical diplexer suitable for deployment in reflector antenna systems is described. The FSS was designed to reflect Ku band signals (11.7–12.75 GHz) while transmitting Ka band signals (17.3–20.2 GHz) and conserving CP in each of these bands. The simulated/measured reflection loss over the Ku band was less than 0.05/0.1 dB for both TE and TM polarizations, while the simulated/measured axial ratio was less than 0.2/0.75 dB. Over the Ka band, the simulated/measured transmission loss for both polarizations was below 0.25/0.4 dB and the simulated/measured axial ratio was less than 0.25/0.75 dB. To the best of our knowledge, this is the first report of a metallo-dielectric FSS that simultaneously operates in CP for an oblique angle of incidence in both Ku and Ka bands.