Application of real-time PCR for total airborne bacterial assessment : comparison with epifluorescence microscopy and culture-dependent methods

Traditional culture-dependent methods to quantify and identify airborne microorganisms are limited by factors such as short-duration sampling times and inability to count nonculturableor non-viable bacteria. Consequently, the quantitative assessment of bioaerosols is often underestimated. Use of the real-time quantitative polymerase chain reaction (Q-PCR) to quantify bacteria in environmental samples presents an alternative method, which should overcome this problem. The aim of this study was to evaluate the performance of a real-time Q-PCR assay as a simple and reliable way to quantify the airborne bacterial load within poultry houses and sewage treatment plants, in comparison with epifluorescencemicroscopy and culture-dependent methods. The estimates of bacterial load that we obtained from real-time PCR and epifluorescence methods, are comparable, however, our analysis of sewage treatment plants indicate these methods give values 270-290 fold greater than those obtained by the ''impaction on nutrient agar'' method. The culture-dependent method of air impaction on nutrient agar was also inadequate in poultry houses, as was the impinger-culture method, which gave a bacterial load estimate 32-fold lower than obtained by Q-PCR. Real-time quantitative PCR thus proves to be a reliable, discerning, and simple method that could be used to estimate airborne bacterial load in a broad variety of other environments expected to carry high numbers of airborne bacteria. [Authors]

Relationship between motion of coronary arteries and diaphragm during free breathing: lessons from real-time MR imaging.

OBJECTIVE: Diaphragmatic navigators are frequently used in free-breathing coronary MR angiography, either to gate or prospectively correct slice position or both. For such approaches, a constant relationship between coronary and diaphragmatic displacement throughout the respiratory cycle is assumed. The purpose of this study was to evaluate the relationship between diaphragmatic and coronary artery motion during free breathing. SUBJECTS AND METHODS: A real-time echoplanar MR imaging sequence was used in 12 healthy volunteers to obtain 30 successive images each (one per cardiac cycle) that included the left main coronary artery and the domes of both hemidiaphragms. The coronary artery and diaphragm positions (relative to isocenter) were determined and analyzed for effective diaphragmatic gating windows of 3, 5, and 7 mm (diaphragmatic excursions of 0-3, 0-5, and 0-7 mm from the end-expiratory position, respectively). RESULTS: Although the mean slope correlating the displacement of the right diaphragm and the left main coronary artery was approximately 0.6 for all diaphragmatic gating windows, we also found great variability among individual volunteers. Linear regression slopes varied from 0.17 to 0.93, and r2 values varied from .04 to .87. CONCLUSION: Wide individual variability exists in the relationship between coronary and diaphragmatic respiratory motion during free breathing. Accordingly, coronary MR angiographic approaches that use diaphragmatic navigator position for prospective slice correction may benefit from patient-specific correction factors. Alternatively, coronary MR angiography may benefit from a more direct assessment of the respiratory displacement of the heart and coronary arteries, using left ventricular navigators.

L’Impacte real de l’Enfocament Basat en els Drets Humans com a eina per a la transformació político-social d’una regió

Aquest estudi pretén donar resposta a la hipòtesi de si l’enfocament basat en drets humans (EBDH) suposa un nou paradigma de treball dins el sistema de cooperació internacional per al desenvolupament per a la veritable transformació socio-política d’una regió. L’estudi en cap cas se centra només en els drets humans, sinó en l’EBDH com una metodologia de treball dins l’àmbit de la cooperació internacional al desenvolupament la qual vol donar resposta a l’objectiu de reduir la pobresa i combatre la injustícia social.

Sistemes encastats en temps real

Resum En l’actualitat, els sistemes electrònics de processament de dades són cada cop més significatius dins del sector industrial. Són moltes les necessitats que sorgeixen en el món dels sistemes d’autentificació, de l’electrònica aeronàutica, d’equips d’emmagatzemament de dades, de telecomunicacions, etc. Aquestes necessitats tecnològiques exigeixen ser controlades per un sistema fiable, robust, totalment dependent amb els esdeveniments externs i que compleixi correctament les restriccions temporals imposades per tal de que realitzi el seu propòsit d’una manera eficient. Aquí és on entren en joc els sistemes encastats en temps real, els quals ofereixen una gran fiabilitat, disponibilitat, una ràpida resposta als esdeveniments externs del sistema, una alta garantia de funcionament i una àmplia possibilitat d’aplicacions. Aquest projecte està pensat per a fer una introducció al món dels sistemes encastats, com també explicar el funcionament del sistema operatiu en temps real FreeRTOS; el qual utilitza com a mètode de programació l’ús de tasques independents entre elles. Donarem una visió de les seves característiques de funcionament, com organitza tasques mitjançant un scheduler i uns exemples per a poder dissenyar-hi aplicacions.

Virtualització de servidors. Avantatges. Implantació en una PIME real

 En la societat d’avui dia, les empreses depenen en gran part dels seus recursos informàtics. La seva capacitat de supervivència i innovació en el mercat actual, on la competitivitat és cada dia més forta, passa per una infraestructura informàtica que els permeti, no només desplegar i implantar ordinadors i servidors de manera ràpida i eficient sinó que també les protegeixi contra parades del sistema informàtic, problemes amb servidors, caigudes o desastres físics de hardware.Per evitar aquests problemes informàtics susceptibles de poder parar el funcionament d’una empresa es va començar a treballar en el camp de la virtualització informàtica amb l’objectiu de poder trobar solucions a aquests problemes a la vegada que s’aprofitaven els recursos de hardware existents d’una manera més òptim a i eficient, reduint així també el cost de la infraestructura informàtica.L’objectiu principal d’aquest treball és veure en primer pla la conversió d’una empresa real amb una infraestructura informàtica del tipus un servidor físic -una funció cap a una infraestructura virtual del tipus un servidor físic -varis servidors virtual -vàries funcions. Analitzarem l’estat actual de l’empresa, servidors i funcions, adquirirem el hardware necessari i farem la conversió de tots els seus servidors cap a una nova infraestructura virtual.Faig especial atenció a les explicacions de perquè utilitzo una opció i no un altre i també procuro sempre donar vàries opcions. Igualment remarco en quadres verds observacions a tenir en compte complementàries al que estic explicant en aquell moment, i en quadres vermells temes en els que s’ha de posar especial atenció en el moment en que es fan. Finalment, un cop feta la conversió, veurem els molts avantatges que ens ha reportat aquesta tecnologia a nivell de fiabilitat, estabilitat, capacitat de tolerància a errades, capacitat de ràpid desplegament de noves màquines, capacitat de recuperació del sistema i aprofitament de recursos físics.

Evapotranspiração real de uma cultura de laranja em produção num Latossolo Vermelho-Amarelo

A disponibilidade de água e de nutrientes no solo em épocas e quantidades apropriadas, associada às condições edáficas e climáticas, é fator de indiscutível relevância à produção das culturas. O objetivo do presente trabalho foi avaliar a evapotranspiração real de uma cultura de laranja, analisar sua variabilidade e verificar a influência deste processo de saída de água do solo na produtividade da cultura. O experimento foi realizado no município de Piracicaba (SP), num Latossolo Vermelho-Amarelo distrófico argissólico, e a evapotranspiração real foi avaliada pelo método do balanço de água no solo em 40 pontos correspondentes a 40 plantas de duas linhas adjacentes com 20 plantas cada. A cultura de laranja, plantada num espaçamento de 7 x 4 m (7 m entre linhas por 4 m entre plantas na linha), tinha 11 anos de idade. Em cada ponto, localizado na projeção da copa (2 m do tronco) de cada uma das 40 plantas ao longo da linha, foram instalados um tubo de Al até à profundidade de 1,2 m para acesso de uma sonda de nêutrons e três tensiômetros às profundidades de 1,0, 1,1 e 1,2 m. A variação da armazenagem de água no solo foi determinada a partir de leituras semanais da sonda de nêutrons. A precipitação pluvial, medida por meio de um pluviômetro com aquisição automática de dados, foi considerada normal para o período monitorado. A drenagem interna e a ascensão capilar foram estimadas pela equação de Darcy-Buckingham, a partir de leituras diárias dos três tensiômetros e da condutividade hidráulica determinada na profundidade de controle (1,1 m) em função do potencial mátrico pelo método do perfil instantâneo. A irrigação e os deflúvios foram inexistentes e a evapotranspiração real da cultura foi calculada pela diferença entre a variação de armazenagem e a soma algébrica dos outros processos avaliados. O balanço foi efetuado durante um ciclo anual da cultura de laranja, dividido em 14 períodos. Nesses 14 períodos de monitoramento, a evapotranspiração real foi analisada com base nos métodos da estatística descritiva e exploratória de dados, obtendo-se um coeficiente de variação médio desses 14 períodos de 16 %. Foi de 1.271 mm a evapotranspiração real anual desta comunidade de plantas cujos valores diários variaram de 0,4 a 8,4 mm. A eficiência de utilização da água ou a razão entre a produtividade e a evapotranspiração real por planta durante o período monitorado variou de 1,57 a 4,52 kg m-3; verificou-se que as plantas com maior produção estavam entre aquelas com maior evapotranspiração real.

Summary of Real Estate Assessment, 2009

The most important aspect of property taxation is the concept that all property should be valued for tax purposes on a uniform basis so that the actual property tax burden can be distributed equitably among individual property owners. One of the most widely used and accepted methods of determining relative levels and uniformity of assessments is the assessment/sales ratio study. Such a study, in its most fundamental analysis, is the comparison of the assessed value of an individual property to its sale price. For example, a property assessed at $12,000 which sold for $26,000 would have an assessment/sales ratio of 46% ($12,000 ÷ $26,000). The purpose of this study is to provide assessment/sales ratio information that may be utilized by property tax administrators, local assessing officials, and interested taxpayers in examining the relative levels and uniformity of assessments throughout the State of Iowa. After further refinement, the study is one factor considered by the Director of Revenue in the biennial equalization of assessments.

Summary of Real Estate Assessment, 2008

The most important aspect of property taxation is the concept that all property should be valued for tax purposes on a uniform basis so that the actual property tax burden can be distributed equitably among individual property owners. One of the most widely used and accepted methods of determining relative levels and uniformity of assessments is the assessment/sales ratio study. Such a study, in its most fundamental analysis, is the comparison of the assessed value of an individual property to its sale price. For example, a property assessed at $12,000 which sold for $26,000 would have an assessment/sales ratio of 46% ($12,000 ÷ $26,000). The purpose of this study is to provide assessment/sales ratio information that may be utilized by property tax administrators, local assessing officials, and interested taxpayers in examining the relative levels and uniformity of assessments throughout the State of Iowa. After further refinement, the study is one factor considered by the Director of Revenue in the biennial equalization of assessments.

Analog circuit for real-time computation of respiratory mechanical impedance in sleep studies

The aim of this work was to develop a low-cost circuit for real-time analog computation of the respiratory mechanical impedance in sleep studies. The practical performance of the circuit was tested in six patients with obstructive sleep apnea. The impedance signal provided by the analog circuit was compared with the impedance calculated simultaneously with a conventional computerized system. We concluded that the low-cost analog circuit developed could be a useful tool for facilitating the real-time assessment of airway obstruction in routine sleep studies.

Ownership structure and innovation: Is there a real link?

This work focuses on the study of the relationship between ownership and control structure of the company and its innovative activity. Its aim consists of analysing the role that may be played by determinants within the company related to ownership structure when the decision to incur research and development activities is taken as well as on the output of this innovate process. Among these determinants we may think of issues such as who owns the firm and how the control of decision-making is distributed, the nature of this control and the level of concentration of ownership, among others. The study is carried out for the year 2001 using a representative sample of Spanish manufacturing industries.

New evidence of the real interest rate parity for OECD countries using panel unit root tests with breaks

This paper tests for real interest parity (RIRP) among the nineteen major OECD countries over the period 1978:Q2-1998:Q4. The econometric methods applied consist of combining the use of several unit root or stationarity tests designed for panels valid under cross-section dependence and presence of multiple structural breaks. Our results strongly support the fulfilment of the weak version of the RIRP for the studied period once dependence and structural breaks are accounted for.

Integración económica y localización industrial. Cataluña, la fábrica de España: ¿cuándo y por qué?

En este artículo se analizan los determinantes de la localización de la actividad industrial en España durante la segunda mitad del siglo XIX. El objetivo es estudiar la existencia de cambios en los factores explicativos de la localización, con el fin de identificar los efectos de la integración económica sobre la geografía industrial española. Para hacerlo, en primer lugar se analiza la literatura histórica existente. En segundo lugar, se describen las teorías que explican la localización de l'actividad. En tercer lugar, se realiza un análisis empírico de la localización y la concentración de la industria española al siglo XIX. A continuación, se propone un estudio econométrico de los determinantes de la localización industrial en dos cortes temporales, 1856 y 1893. Los resultados son consistentes con las teorías del comercio. Durante el decurso de la segunda mitad del siglo XIX, España paso a ser una economía integrada, la movilidad del trabajo y del capital se vio favorecida y como resultado, las diferencias relativas en las dotaciones de factores perdieron importancia en la explicación de las pautas de especialización industrial. Por el contrario, la integración económica favoreció la significación relativa de las economías de escala o de la proximidad a los mercados como elementos explicativos de la localización de la industria, favoreciendo, de esta manera, la aglomeración de la actividad industrial

New evidence of the real interest rate parity for OECD countries using panel unit root tests with breaks

This paper tests for real interest parity (RIRP) among the nineteen major OECD countries over the period 1978:Q2-1998:Q4. The econometric methods applied consist of combining the use of several unit root or stationarity tests designed for panels valid under cross-section dependence and presence of multiple structural breaks. Our results strongly support the fulfilment of the weak version of the RIRP for the studied period once dependence and structural breaks are accounted for.