Helsinki 31/6 -28

Kirje 31.6.1928

Kirjailija Arvo Salo 2/5 1972 Helsinki

Kirje 2.5.1972

Helsinki 10.8.1940

Kirje

Helsinki 8.3.1932

Kirje

Helsinki 28.5.1925

Kirje

Helsinki 26.11.1924

Kirje

RecA-mediated strand exchange traverses substitutional heterologies more easily than deletions or insertions.

RecA protein in bacteria and its eukaryotic homolog Rad51 protein are responsible for initiation of strand exchange between homologous DNA molecules. This process is crucial for homologous recombination, the repair of certain types of DNA damage and for the reinitiation of DNA replication on collapsed replication forks. We show here, using two different types of in vitro assays, that in the absence of ATP hydrolysis RecA-mediated strand exchange traverses small substitutional heterologies between the interacting DNAs, whereas small deletions or insertions block the ongoing strand exchange. We discuss evolutionary implications of RecA selectivity against insertions and deletions and propose a molecular mechanism by which RecA can exert this selectivity.

Helsinki 5.7.1925

Kirje

Helsinki 15.6.1925

Kirje

Helsinki 7/3 1940

Kirje 7.3.1949

[Helsinki] 7 p:nä syyskuuta 1942

Kirje 7.9.1942