RecA-mediated strand exchange traverses substitutional heterologies more easily than deletions or insertions.


Autoria(s): Bucka A.; Stasiak A.
Data(s)

01/06/2001

Resumo

RecA protein in bacteria and its eukaryotic homolog Rad51 protein are responsible for initiation of strand exchange between homologous DNA molecules. This process is crucial for homologous recombination, the repair of certain types of DNA damage and for the reinitiation of DNA replication on collapsed replication forks. We show here, using two different types of in vitro assays, that in the absence of ATP hydrolysis RecA-mediated strand exchange traverses small substitutional heterologies between the interacting DNAs, whereas small deletions or insertions block the ongoing strand exchange. We discuss evolutionary implications of RecA selectivity against insertions and deletions and propose a molecular mechanism by which RecA can exert this selectivity.

Identificador

http://serval.unil.ch/?id=serval:BIB_769B9002A891

isbn:1362-4962[electronic], 0305-1048[linking]

pmid:11410652

doi:10.1093/nar/29.12.2464

isiid:000169616100004

Idioma(s)

en

Fonte

Nucleic Acids Research, vol. 29, no. 12, pp. 2464-2470

Palavras-Chave #Adenosine Triphosphate/metabolism; Adenylyl Imidodiphosphate/metabolism; Artifacts; DNA Repair/genetics; DNA Replication/genetics; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Hydrolysis; Models, Biological; Mutagenesis, Insertional/genetics; Nucleic Acid Conformation; Oligodeoxyribonucleotides/chemistry; Oligodeoxyribonucleotides/genetics; Rec A Recombinases/metabolism; Recombination, Genetic/genetics; Sequence Deletion/genetics; Substrate Specificity
Tipo

info:eu-repo/semantics/article

article